LOCUS       NM_001430               5155 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens endothelial PAS domain protein 1 (EPAS1), mRNA.
ACCESSION   NM_001430
VERSION     NM_001430.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Messe,M., Bernhard,C., Foppolo,S., Thomas,L., Marchand,P.,
            Herold-Mende,C., Idbaih,A., Kessler,H., Etienne-Selloum,N.,
            Ochoa,C., Tambar,U.K., Elati,M., Laquerriere,P., Entz-Werle,N.,
            Martin,S., Reita,D. and Dontenwill,M.
  TITLE     Hypoxia-driven heterogeneous expression of alpha5 integrin in
            glioblastoma stem cells is linked to HIF-2alpha
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (8), 167471 (2024)
   PUBMED   39154793
  REMARK    GeneRIF: Hypoxia-driven heterogeneous expression of alpha5 integrin
            in glioblastoma stem cells is linked to HIF-2alpha.
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Zarychta,J., Kowalczyk,A., Slowik,K., Przywara,D., Petniak,A.,
            Kondracka,A., Wojtowicz-Marzec,M., Slyk-Gulewska,P.,
            Kwasniewska,A., Kocki,J. and Gil-Kulik,P.
  TITLE     Pilot Study on the Effect of Patient Condition and Clinical
            Parameters on Hypoxia-Induced Factor Expression: HIF1A, EPAS1 and
            HIF3A in Human Colostrum Cells
  JOURNAL   Int J Mol Sci 25 (20), 11042 (2024)
   PUBMED   39456823
  REMARK    GeneRIF: Pilot Study on the Effect of Patient Condition and
            Clinical Parameters on Hypoxia-Induced Factor Expression: HIF1A,
            EPAS1 and HIF3A in Human Colostrum Cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5155)
  AUTHORS   Ohgaki,R., Hirase,Y., Xu,M., Okanishi,H. and Kanai,Y.
  TITLE     LAT1 expression in colorectal cancer cells is unresponsive to
            HIF-1/2alpha accumulation under experimental hypoxia
  JOURNAL   Sci Rep 14 (1), 19635 (2024)
   PUBMED   39179631
  REMARK    GeneRIF: LAT1 expression in colorectal cancer cells is unresponsive
            to HIF-1/2alpha accumulation under experimental hypoxia.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5155)
  AUTHORS   Shen,J., Lai,Y., Lu,Y., Liu,Y., Zhang,J., Wu,Y., Pan,Y., Chen,H.,
            Gao,Q., Wei,Q., Chen,Y., Ye,J., Lin,Y., Liu,B., Jiang,J. and Nan,J.
  TITLE     NRF2-HIF2alpha Signaling Attenuates Endothelial Cell Senescence and
            Maintains Intercellular Junctions in Diabetes
  JOURNAL   Int J Biol Sci 20 (10), 4055-4073 (2024)
   PUBMED   39113713
  REMARK    GeneRIF: NRF2-HIF2alpha Signaling Attenuates Endothelial Cell
            Senescence and Maintains Intercellular Junctions in Diabetes.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5155)
  AUTHORS   Bacigalupa,Z.A., Arner,E.N., Vlach,L.M., Wolf,M.M., Brown,W.A.,
            Krystofiak,E.S., Ye,X., Hongo,R.A., Landis,M., Amason,E.K.,
            Beckermann,K.E., Rathmell,W.K. and Rathmell,J.C.
  TITLE     HIF-2alpha expression and metabolic signaling require ACSS2 in
            clear cell renal cell carcinoma
  JOURNAL   J Clin Invest 134 (12), e164249 (2024)
   PUBMED   38941296
  REMARK    GeneRIF: HIF-2alpha expression and metabolic signaling require
            ACSS2 in clear cell renal cell carcinoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5155)
  AUTHORS   Cockman,M.E., Masson,N., Mole,D.R., Jaakkola,P., Chang,G.W.,
            Clifford,S.C., Maher,E.R., Pugh,C.W., Ratcliffe,P.J. and
            Maxwell,P.H.
  TITLE     Hypoxia inducible factor-alpha binding and ubiquitylation by the
            von Hippel-Lindau tumor suppressor protein
  JOURNAL   J Biol Chem 275 (33), 25733-25741 (2000)
   PUBMED   10823831
REFERENCE   7  (bases 1 to 5155)
  AUTHORS   Ema,M., Hirota,K., Mimura,J., Abe,H., Yodoi,J., Sogawa,K.,
            Poellinger,L. and Fujii-Kuriyama,Y.
  TITLE     Molecular mechanisms of transcription activation by HLF and
            HIF1alpha in response to hypoxia: their stabilization and redox
            signal-induced interaction with CBP/p300
  JOURNAL   EMBO J 18 (7), 1905-1914 (1999)
   PUBMED   10202154
REFERENCE   8  (bases 1 to 5155)
  AUTHORS   Hogenesch,J.B., Gu,Y.Z., Jain,S. and Bradfield,C.A.
  TITLE     The basic-helix-loop-helix-PAS orphan MOP3 forms transcriptionally
            active complexes with circadian and hypoxia factors
  JOURNAL   Proc Natl Acad Sci U S A 95 (10), 5474-5479 (1998)
   PUBMED   9576906
REFERENCE   9  (bases 1 to 5155)
  AUTHORS   Hogenesch,J.B., Chan,W.K., Jackiw,V.H., Brown,R.C., Gu,Y.Z.,
            Pray-Grant,M., Perdew,G.H. and Bradfield,C.A.
  TITLE     Characterization of a subset of the basic-helix-loop-helix-PAS
            superfamily that interacts with components of the dioxin signaling
            pathway
  JOURNAL   J Biol Chem 272 (13), 8581-8593 (1997)
   PUBMED   9079689
REFERENCE   10 (bases 1 to 5155)
  AUTHORS   Tian,H., McKnight,S.L. and Russell,D.W.
  TITLE     Endothelial PAS domain protein 1 (EPAS1), a transcription factor
            selectively expressed in endothelial cells
  JOURNAL   Genes Dev 11 (1), 72-82 (1997)
   PUBMED   9000051
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC016696.8, CD109908.1,
            BC051338.1 and AK123845.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001430.4.
            
            Summary: This gene encodes a transcription factor involved in the
            induction of genes regulated by oxygen, which is induced as oxygen
            levels fall. The encoded protein contains a basic-helix-loop-helix
            domain protein dimerization domain as well as a domain found in
            proteins in signal transduction pathways which respond to oxygen
            levels. Mutations in this gene are associated with erythrocytosis
            familial type 4. [provided by RefSeq, Nov 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.156330.1,
                                           SRR1660803.67820.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263734.5/ ENSP00000263734.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AC016696.8         81227-81243
            18-531              CD109908.1         50-563
            532-3656            BC051338.1         342-3466
            3657-4270           AK123845.1         2125-2738
            4271-5155           BC051338.1         4079-4963
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p21"
     gene            1..5155
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /note="endothelial PAS domain protein 1"
                     /db_xref="GeneID:2034"
                     /db_xref="HGNC:HGNC:3374"
                     /db_xref="MIM:603349"
     exon            1..531
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             506..3118
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /note="HIF-1alpha-like factor; member of PAS protein 2;
                     hypoxia-inducible factor 2 alpha;
                     basic-helix-loop-helix-PAS protein MOP2; EPAS-1;
                     HIF2-alpha; HIF-2-alpha; HIF-1-alpha-like factor; PAS
                     domain-containing protein 2; class E basic
                     helix-loop-helix protein 73"
                     /codon_start=1
                     /product="endothelial PAS domain-containing protein 1"
                     /protein_id="NP_001421.2"
                     /db_xref="CCDS:CCDS1825.1"
                     /db_xref="GeneID:2034"
                     /db_xref="HGNC:HGNC:3374"
                     /db_xref="MIM:603349"
                     /translation="MTADKEKKRSSSERRKEKSRDAARCRRSKETEVFYELAHELPLP
                     HSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEAEADQQMDNLYLKALEGFIAVV
                     TQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLSLKNGSGFGKKSK
                     DMSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVKVYNNCPPHNSLCGYKEPL
                     LSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRITELIGYHPEELLGRS
                     AYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQP
                     QCIMCVNYVLSEIEKNDVVFSMDQTESLFKPHLMAMNSIFDSSGKGAVSEKSNFLFTK
                     LKEEPEELAQLAPTPGDAIISLDFGNQNFEESSAYGKAILPPSQPWATELRSHSTQSE
                     AGSLPAFTVPQAAAPGSTTPSATSSSSSCSTPNSPEDYYTSLDNDLKIEVIEKLFAMD
                     TEAKDQCSTQTDFNELDLETLAPYIPMDGEDFQLSPICPEERLLAENPQSTPQHCFSA
                     MTNIFQPLAPVAPHSPFLLDKFQQQLESKKTEPEHRPMSSIFFDAGSKASLPPCCGQA
                     STPLSSMGGRSNTQWPPDPPLHFGPTKWAVGDQRTEFLGAAPLGPPVSPPHVSTFKTR
                     SAKGFGARGPDVLSPAMVALSNKLKLKRQLEYEEQAFQDLSGGDPPGGSTSHLMWKRM
                     KNLRGGSCPLMPDKPLSANVPNDKFTQNPMRGLGHPLRHLPLPQPPSAISPGENSKSR
                     FPPQCYATQYQDYSLSSAHKVSGMASRLLGPSFESYLLPELTRYDCEVNVPVLGSSTL
                     LQGGDLLRALDQAT"
     exon            532..722
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     exon            723..874
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     exon            875..959
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1078
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1284
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1391
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1539
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1754
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1948
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2059
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..2550
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2551..2677
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2678..2792
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2793..2966
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2967..5155
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5136..5141
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /note="hexamer: ATTAAA"
     polyA_site      5155
                     /gene="EPAS1"
                     /gene_synonym="bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2"
                     /note="major polyA site"
ORIGIN      
        1 acactcgcga gcggaccgcc acacgggtcc ggtgcccgct gcgcttccgc cccagcgctc
       61 ctgaggcggc cgtacaatcc tcggcagtgt cctgagactg tatggtcagc tcagcccggc
      121 ctccgactcc ttccgactcc cagcattcga gccacttttt tttttctttg aaaactcaga
      181 aaagtgactc cttttccagg gaaaaaggaa cttgggttcc cttctctccg tcctcttttc
      241 gggtctgaca gcctccaccc actccttccc cggaccccgc ctccgcgcgc aggttcctcc
      301 cagtcacctt tctccacccc cgcccccgca cctagcccgc cgcgcgccac cttccacctg
      361 actgcgcggg gcgctcggga cctgcgcgca cctcggacct tcaccacccg cccgggccgc
      421 ggggagcgga cgagggccac agccccccac ccgccaggga gcccaggtgc tcggcgtctg
      481 aacgtctcaa agggccacag cgacaatgac agctgacaag gagaagaaaa ggagtagctc
      541 ggagaggagg aaggagaagt cccgggatgc tgcgcggtgc cggcggagca aggagacgga
      601 ggtgttctat gagctggccc atgagctgcc tctgccccac agtgtgagct cccatctgga
      661 caaggcctcc atcatgcgac tggcaatcag cttcctgcga acacacaagc tcctctcctc
      721 agtttgctct gaaaacgagt ccgaagccga agctgaccag cagatggaca acttgtacct
      781 gaaagccttg gagggtttca ttgccgtggt gacccaagat ggcgacatga tctttctgtc
      841 agaaaacatc agcaagttca tgggacttac acaggtggag ctaacaggac atagtatctt
      901 tgacttcact catccctgcg accatgagga gattcgtgag aacctgagtc tcaaaaatgg
      961 ctctggtttt gggaaaaaaa gcaaagacat gtccacagag cgggacttct tcatgaggat
     1021 gaagtgcacg gtcaccaaca gaggccgtac tgtcaacctc aagtcagcca cctggaaggt
     1081 cttgcactgc acgggccagg tgaaagtcta caacaactgc cctcctcaca atagtctgtg
     1141 tggctacaag gagcccctgc tgtcctgcct catcatcatg tgtgaaccaa tccagcaccc
     1201 atcccacatg gacatccccc tggatagcaa gaccttcctg agccgccaca gcatggacat
     1261 gaagttcacc tactgtgatg acagaatcac agaactgatt ggttaccacc ctgaggagct
     1321 gcttggccgc tcagcctatg aattctacca tgcgctagac tccgagaaca tgaccaagag
     1381 tcaccagaac ttgtgcacca agggtcaggt agtaagtggc cagtaccgga tgctcgcaaa
     1441 gcatgggggc tacgtgtggc tggagaccca ggggacggtc atctacaacc ctcgcaacct
     1501 gcagccccag tgcatcatgt gtgtcaacta cgtcctgagt gagattgaga agaatgacgt
     1561 ggtgttctcc atggaccaga ctgaatccct gttcaagccc cacctgatgg ccatgaacag
     1621 catctttgat agcagtggca agggggctgt gtctgagaag agtaacttcc tattcaccaa
     1681 gctaaaggag gagcccgagg agctggccca gctggctccc accccaggag acgccatcat
     1741 ctctctggat ttcgggaatc agaacttcga ggagtcctca gcctatggca aggccatcct
     1801 gcccccgagc cagccatggg ccacggagtt gaggagccac agcacccaga gcgaggctgg
     1861 gagcctgcct gccttcaccg tgccccaggc agctgccccg ggcagcacca cccccagtgc
     1921 caccagcagc agcagcagct gctccacgcc caatagccct gaagactatt acacatcttt
     1981 ggataacgac ctgaagattg aagtgattga gaagctcttc gccatggaca cagaggccaa
     2041 ggaccaatgc agtacccaga cggatttcaa tgagctggac ttggagacac tggcacccta
     2101 tatccccatg gacggggaag acttccagct aagccccatc tgccccgagg agcggctctt
     2161 ggcggagaac ccacagtcca ccccccagca ctgcttcagt gccatgacaa acatcttcca
     2221 gccactggcc cctgtagccc cgcacagtcc cttcctcctg gacaagtttc agcagcagct
     2281 ggagagcaag aagacagagc ccgagcaccg gcccatgtcc tccatcttct ttgatgccgg
     2341 aagcaaagca tccctgccac cgtgctgtgg ccaggccagc acccctctct cttccatggg
     2401 gggcagatcc aatacccagt ggcccccaga tccaccatta cattttgggc ccacaaagtg
     2461 ggccgtcggg gatcagcgca cagagttctt gggagcagcg ccgttggggc cccctgtctc
     2521 tccaccccat gtctccacct tcaagacaag gtctgcaaag ggttttgggg ctcgaggccc
     2581 agacgtgctg agtccggcca tggtagccct ctccaacaag ctgaagctga agcgacagct
     2641 ggagtatgaa gagcaagcct tccaggacct gagcgggggg gacccacctg gtggcagcac
     2701 ctcacatttg atgtggaaac ggatgaagaa cctcaggggt gggagctgcc ctttgatgcc
     2761 ggacaagcca ctgagcgcaa atgtacccaa tgataagttc acccaaaacc ccatgagggg
     2821 cctgggccat cccctgagac atctgccgct gccacagcct ccatctgcca tcagtcccgg
     2881 ggagaacagc aagagcaggt tccccccaca gtgctacgcc acccagtacc aggactacag
     2941 cctgtcgtca gcccacaagg tgtcaggcat ggcaagccgg ctgctcgggc cctcatttga
     3001 gtcctacctg ctgcccgaac tgaccagata tgactgtgag gtgaacgtgc ccgtgctggg
     3061 aagctccacg ctcctgcaag gaggggacct cctcagagcc ctggaccagg ccacctgagc
     3121 caggccttct acctgggcag cacctctgcc gacgccgtcc caccagcttc actctctccg
     3181 tctgtttttg caactaggta tttctaacgc cagcacacta tttacaagat ggacttacct
     3241 ggcagacttg cccaggtcac caagcagtgg cctttttctg agatgctcac tttattatcc
     3301 ctatttttaa agtacacaat tgttttacct gttctgaaat gttcttaaat tttgtaggat
     3361 ttttttcctc cccaccttca atgacttcta atttatatta tccataggtt tctctccctc
     3421 cttctccttc tcacacacaa ctgtccatac taacaagttt ggtgcatgtc tgttcttctg
     3481 tagggagaag ctttagcttc attttactaa aaagattcct cgttattgtt gttgccaaag
     3541 agaaacaaaa atgattttgc tttccaagct tggtttgtgg cgtctccctc gcagagccct
     3601 tctcgtttct tttttaaact aatcaccata ttgtaaattt cagggttttt ttttttttgt
     3661 ttaagctgac tctttgctct aattttggaa aaaaagaaat gtgaagggtc aactccaacg
     3721 tatgtggtta tctgtgaaag ttgcacagcg tggcttttcc taaactggtg tttttccccc
     3781 gcatttggtg gattttttat tattattcaa aaacataact gagtttttta aaagaggaga
     3841 aaatttatat ctgggttaag tgtttatcat atatatgggt actttgtaat atctaaaaac
     3901 ttagaaacgg aaatggaatc ctgctcacaa aatcacttta agatcttttc gaagctgtta
     3961 atttttctta gtgttgtgga cactgcagac ttgtccagtg ctcccacggc ctgtacggac
     4021 actgtggaag gcctccctct gtcggctttt tgccatctgt gatatgccat aggtgtgaca
     4081 atccgagcag tggagtcatt cagcgggagc actgcgcgct atcccctcac attctctatg
     4141 tactatgtat gtatgtatta ttattattgc tgccaagagg gtctgatggc acgttgtggg
     4201 gtcggggggt ggggcgggga agtgctctaa cttttcttaa ggttttgttg ctagcccttc
     4261 aagtgcactg agctatgtga ctcggatggt ctttcacacg gcacatttgg acatttccag
     4321 aactaccatg agatggttta gacgggaatt catgcaaatg aggggtcaaa aatggtatag
     4381 tgaccccgtc cacgtcctcc aagctcacga ccttggagcc ccgtggagct ggactgagga
     4441 ggaggctgca cagcgggaga gcagctggtc cagaccagcc ctgcagcccc cactcagccg
     4501 gcagccagat ggccccgcaa ggcctccagg gatggcccct agccacaggc cctggctgag
     4561 gtctctgggt cggtcagtga catgtaggta ggaagcactg aaaatagtgt tcccagagca
     4621 ctttgcaact ccctgggtaa gagggacgac acctctggtt tttcaatacc aattacatgg
     4681 aacttttctg taatgggtac aatgaagaag tttctaaaaa cacacacaaa gcacattggg
     4741 ccaactattt agtaagcccg gatagactta ttgccaaaaa caaaaaatag ctttcaaaag
     4801 aaatttaagt tctatgagaa attccttagt catggtgttg cgtaaatcat attttagctg
     4861 cacggcatta ccccacacag ggtggcagaa cttgaagggt tactgacgtg taaatgctgg
     4921 tatttgattt cctgtgtgtg ttgccctggc attaagggca ttttaccctt gcagttttac
     4981 taaaacactg aaaaatattc caagcttcat attaacccta cctgtcaacg taacgatttc
     5041 atgaacgtta ttatattgtc gaattcctac tgacaacatt ataactgtat gggagcttaa
     5101 ctttataagg aaatgtattt tgacactggt atcttattaa agtattctga tccta
//
