LOCUS       NM_001033113            2130 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens ectonucleoside triphosphate diphosphohydrolase 8
            (ENTPD8), transcript variant 1, mRNA.
ACCESSION   NM_001033113
VERSION     NM_001033113.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Munkonda,M.N., Kauffenstein,G., Kukulski,F., Levesque,S.A.,
            Legendre,C., Pelletier,J., Lavoie,E.G., Lecka,J. and Sevigny,J.
  TITLE     Inhibition of human and mouse plasma membrane bound NTPDases by P2
            receptor antagonists
  JOURNAL   Biochem Pharmacol 74 (10), 1524-1534 (2007)
   PUBMED   17727821
REFERENCE   3  (bases 1 to 2130)
  AUTHORS   Fausther,M., Lecka,J., Kukulski,F., Levesque,S.A., Pelletier,J.,
            Zimmermann,H., Dranoff,J.A. and Sevigny,J.
  TITLE     Cloning, purification, and identification of the liver canalicular
            ecto-ATPase as NTPDase8
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 292 (3), G785-G795 (2007)
   PUBMED   17095758
  REMARK    GeneRIF: NTPDase8 is the canalicular ecto-ATPase/ATPDase and is
            responsible for the main hepatic NTPDase activity.
REFERENCE   4  (bases 1 to 2130)
  AUTHORS   Knowles,A.F. and Li,C.
  TITLE     Molecular cloning and characterization of expressed human
            ecto-nucleoside triphosphate diphosphohydrolase 8 (E-NTPDase 8) and
            its soluble extracellular domain
  JOURNAL   Biochemistry 45 (23), 7323-7333 (2006)
   PUBMED   16752921
REFERENCE   5  (bases 1 to 2130)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX322799.22.
            
            On Aug 22, 2019 this sequence version replaced NM_001033113.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.2862998.1,
                                           SRR11853562.32674.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968540,
                                           SAMEA1968832 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371506.7/ ENSP00000360561.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                BX322799.22        137955-138026       c
            73-218              BX322799.22        134829-134974       c
            219-336             BX322799.22        134636-134753       c
            337-487             BX322799.22        133828-133978       c
            488-647             BX322799.22        133538-133697       c
            648-878             BX322799.22        133190-133420       c
            879-1142            BX322799.22        132682-132945       c
            1143-1253           BX322799.22        132388-132498       c
            1254-1388           BX322799.22        131873-132007       c
            1389-2130           BX322799.22        131033-131774       c
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.3"
     gene            1..2130
                     /gene="ENTPD8"
                     /gene_synonym="E-NTPDase; GLSR2492; NTPDase-8; UNQ2492"
                     /note="ectonucleoside triphosphate diphosphohydrolase 8"
                     /db_xref="GeneID:377841"
                     /db_xref="HGNC:HGNC:24860"
                     /db_xref="MIM:616748"
     exon            1..72
                     /gene="ENTPD8"
                     /gene_synonym="E-NTPDase; GLSR2492; NTPDase-8; UNQ2492"
                     /inference="alignment:Splign:2.1.0"
     exon            73..218
                     /gene="ENTPD8"
                     /gene_synonym="E-NTPDase; GLSR2492; NTPDase-8; UNQ2492"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..1580
                     /gene="ENTPD8"
                     /gene_synonym="E-NTPDase; GLSR2492; NTPDase-8; UNQ2492"
                     /EC_number="3.6.1.5"
                     /note="isoform 1 is encoded by transcript variant 1; liver
                     ecto-ATP-diphosphohydrolase; E-NTPDase 8"
                     /codon_start=1
                     /product="ectonucleoside triphosphate diphosphohydrolase 8
                     isoform 1"
                     /protein_id="NP_001028285.1"
                     /db_xref="CCDS:CCDS43913.1"
                     /db_xref="GeneID:377841"
                     /db_xref="HGNC:HGNC:24860"
                     /db_xref="MIM:616748"
                     /translation="MGLSRKEQVFLALLGASGVSGLTALILLLVEATSVLLPTDIKFG
                     IVFDAGSSHTSLFLYQWLANKENGTGVVSQALACQVEGPGISSYTSNAAQAGESLQGC
                     LEEALVLIPEAQHRKTPTFLGATAGMRLLSRKNSSQARDIFAAVTQVLGRSPVDFWGA
                     ELLAGQAEGAFGWITVNYGLGTLVKYSFTGEWIQPPEEMLVGALDMGGASTQITFVPG
                     GPILDKSTQADFRLYGSDYSVYTHSYLCFGRDQMLSRLLVGLVQSRPAALLRHPCYLS
                     GYQTTLALGPLYESPCVHATPPLSLPQNLTVEGTGNPGACVSAIRELFNFSSCQGQED
                     CAFDGVYQPPLRGQFYAFSNFYYTFHFLNLTSRQPLSTVNATIWEFCQRPWKLVEASY
                     PGQDRWLRDYCASGLYILTLLHEGYGFSEETWPSLEFRKQAGGVDIGWTLGYMLNLTG
                     MIPADAPAQWRAESYGVWVAKVVFMVLALVAVVGAALVQLFWLQD"
     exon            219..336
                     /gene="ENTPD8"
                     /gene_synonym="E-NTPDase; GLSR2492; NTPDase-8; UNQ2492"
                     /inference="alignment:Splign:2.1.0"
     exon            337..487
                     /gene="ENTPD8"
                     /gene_synonym="E-NTPDase; GLSR2492; NTPDase-8; UNQ2492"
                     /inference="alignment:Splign:2.1.0"
     exon            488..647
                     /gene="ENTPD8"
                     /gene_synonym="E-NTPDase; GLSR2492; NTPDase-8; UNQ2492"
                     /inference="alignment:Splign:2.1.0"
     exon            648..878
                     /gene="ENTPD8"
                     /gene_synonym="E-NTPDase; GLSR2492; NTPDase-8; UNQ2492"
                     /inference="alignment:Splign:2.1.0"
     exon            879..1142
                     /gene="ENTPD8"
                     /gene_synonym="E-NTPDase; GLSR2492; NTPDase-8; UNQ2492"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1253
                     /gene="ENTPD8"
                     /gene_synonym="E-NTPDase; GLSR2492; NTPDase-8; UNQ2492"
                     /inference="alignment:Splign:2.1.0"
     exon            1254..1388
                     /gene="ENTPD8"
                     /gene_synonym="E-NTPDase; GLSR2492; NTPDase-8; UNQ2492"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..2130
                     /gene="ENTPD8"
                     /gene_synonym="E-NTPDase; GLSR2492; NTPDase-8; UNQ2492"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2107..2112
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENTPD8"
                     /gene_synonym="E-NTPDase; GLSR2492; NTPDase-8; UNQ2492"
                     /note="hexamer: ATTAAA"
     polyA_site      2130
                     /gene="ENTPD8"
                     /gene_synonym="E-NTPDase; GLSR2492; NTPDase-8; UNQ2492"
                     /note="major polyA site"
ORIGIN      
        1 aggtgcgcgg acacagaagc gtctaagcac agcttcctcc ttgccgctcc gggaagtggg
       61 cagccagccc aggaaccagt accacctgca ccatggggct gtcccggaag gagcaggtct
      121 tcttggccct gctgggggcc tcgggggtct caggcctcac ggcactcatt ctcctcctgg
      181 tggaggccac cagcgtgctc ctgcccacag acatcaagtt tgggatcgtg tttgatgcgg
      241 gctcctccca cacgtccctc ttcctgtatc agtggctggc gaacaaggag aatggcacgg
      301 gtgtggtcag ccaggccctg gcctgccagg tggaagggcc tggaatctcc tcctacactt
      361 ctaatgctgc acaggctggt gagagcctgc agggctgctt ggaggaggcg ctggtgctga
      421 tcccagaggc ccagcatcgg aaaacaccca cgttcctggg ggccacggct ggcatgaggt
      481 tgctcagccg gaagaacagc tctcaggcca gggacatctt tgcagcagtc acccaggtcc
      541 tgggccggtc tcccgtggac ttttggggtg ccgagctcct ggccgggcag gccgaaggtg
      601 cctttggttg gatcactgtc aactacggct tggggacgct ggtcaagtac tccttcactg
      661 gagaatggat ccagcctccg gaggagatgc tggtgggtgc cctggacatg ggaggggcct
      721 ccacccagat cacgttcgtg cctgggggcc ccatcttgga caagagcacc caggccgatt
      781 ttcgcctcta cggctccgac tacagcgtct acactcacag ctacctgtgc tttggacggg
      841 accagatgct gagcaggctc ctcgtggggc tggtacagag ccgcccggct gccctgctcc
      901 gtcacccgtg ctacctcagc ggctaccaga ccacactggc cctgggcccg ctgtatgagt
      961 caccctgtgt ccacgccacg cccccgctga gcctccccca gaacctcaca gttgaaggga
     1021 caggcaaccc tggagcctgc gtctcagcca tccgggaact tttcaacttc tccagctgcc
     1081 agggccagga ggactgcgcc tttgacgggg tctaccagcc cccgctgcgg ggccagttct
     1141 atgccttctc caacttctac tacaccttcc acttcctgaa cctcacctcc aggcagcccc
     1201 tgagcacggt caacgccacc atctgggagt tttgccagag gccctggaaa ctggtggagg
     1261 ccagctaccc tgggcaggac cgctggctgc gggactactg tgcctcaggc ctgtacatcc
     1321 tcaccctcct gcacgagggc tacgggttca gcgaggagac ctggcccagc ctcgagttcc
     1381 gaaagcaggc gggcggtgtg gacattggct ggacactggg ctacatgctg aacctgaccg
     1441 ggatgatccc ggccgatgcg ccggctcagt ggcgggcaga gagctacggc gtctgggtgg
     1501 ccaaagtggt gttcatggtg ctggccctgg tggcggtggt gggggctgcc ttggtccagc
     1561 tcttctggtt gcaggactag tgggaaggcg gaggtgggcc cccacagagc ccacaggcag
     1621 ctgcgtcccg gatgctggag gcttcctgag ccctgagcgc cgtggggcct tgctctgtgg
     1681 ctctgcccac ggtcaggtga cagccacctc cagggcaccg tcagggtggt gctggccaca
     1741 gaggctgcat gacctcccct cccggcgtcc ctcccccaac ctccttccgc aactgggctt
     1801 ccagggccgt aggtgccttt ctgcacacag gccgccagga ctcgtggtgt ctccaggctg
     1861 tgtgactgca gggccacatg ctgcctgcaa acagggcaag accacggagg cacaggggtc
     1921 ctgctcctga tggggcctca ggaggggcgg agaggggtgg aagggaggga gctgccccac
     1981 ctggaccccc gctctccctg ctgttgtctg agcagatgga tggagtccag gcctgggggc
     2041 ttctgctggg ccagcccggc ctcccacacc cacttggagg gtgagactgc agtgggggtt
     2101 gtttttatta aaagcatcat ggacacagca
//
