LOCUS       NM_001248               2916 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ectonucleoside triphosphate diphosphohydrolase 3
            (ENTPD3), transcript variant 2, mRNA.
ACCESSION   NM_001248
VERSION     NM_001248.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Noronha-Matos,J.B., Pinto-Cardoso,R., Bessa-Andres,C.,
            Magalhaes-Cardoso,M.T., Ferreirinha,F., Costa,M.A., Marinhas,J.,
            Freitas,R., Lemos,R., Vilaca,A., Oliveira,A., Pelletier,J.,
            Sevigny,J. and Correia-de-Sa,P.
  TITLE     Silencing NTPDase3 activity rehabilitates the osteogenic commitment
            of post-menopausal stem cell bone progenitors
  JOURNAL   Stem Cell Res Ther 14 (1), 97 (2023)
   PUBMED   37076930
  REMARK    GeneRIF: Silencing NTPDase3 activity rehabilitates the osteogenic
            commitment of post-menopausal stem cell bone progenitors.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Docherty,F.M., Riemondy,K.A., Castro-Gutierrez,R., Dwulet,J.M.,
            Shilleh,A.H., Hansen,M.S., Williams,S.P.M., Armitage,L.H.,
            Santostefano,K.E., Wallet,M.A., Mathews,C.E., Triolo,T.M.,
            Benninger,R.K.P. and Russ,H.A.
  TITLE     ENTPD3 Marks Mature Stem Cell-Derived beta-Cells Formed by
            Self-Aggregation In Vitro
  JOURNAL   Diabetes 70 (11), 2554-2567 (2021)
   PUBMED   34380694
  REMARK    GeneRIF: ENTPD3 Marks Mature Stem Cell-Derived beta-Cells Formed by
            Self-Aggregation In Vitro.
REFERENCE   3  (bases 1 to 2916)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2916)
  AUTHORS   Feldbrugge,L., Moss,A.C., Yee,E.U., Csizmadia,E., Mitsuhashi,S.,
            Longhi,M.S., Sandhu,B., Stephan,H., Wu,Y., Cheifetz,A.S.,
            Muller,C.E., Sevigny,J., Robson,S.C. and Jiang,Z.G.
  TITLE     Expression of Ecto-nucleoside Triphosphate Diphosphohydrolases-2
            and -3 in the Enteric Nervous System Affects Inflammation in
            Experimental Colitis and Crohn's Disease
  JOURNAL   J Crohns Colitis 11 (9), 1113-1123 (2017)
   PUBMED   28472257
  REMARK    GeneRIF: NTPDase2 and -3 are ecto-enzymes expressed in the enteric
            nervous system. Both enzymes confer protection against gut
            inflammation in experimental colitis and exhibit alterations in
            Crohn's disease. These observations suggest that purinergic
            signalling modulated by E-NTPDases governs neuro-immune
            interactions that are relevant in Crohn's disease.
REFERENCE   5  (bases 1 to 2916)
  AUTHORS   Roszek,K., Porowinska,D., Bajek,A., Holysz,M. and Czarnecka,J.
  TITLE     Chondrogenic Differentiation of Human Mesenchymal Stem Cells
            Results in Substantial Changes of Ecto-Nucleotides Metabolism
  JOURNAL   J Cell Biochem 116 (12), 2915-2923 (2015)
   PUBMED   26018728
  REMARK    GeneRIF: Despite the increased level of NTPDase1 and NTPDase3 mRNA
            expression in chondrogenically induced MSCs, their activity toward
            ATP remains quite low.
REFERENCE   6  (bases 1 to 2916)
  AUTHORS   Yang,F., Hicks-Berger,C.A., Smith,T.M. and Kirley,T.L.
  TITLE     Site-directed mutagenesis of human nucleoside triphosphate
            diphosphohydrolase 3: the importance of residues in the apyrase
            conserved regions
  JOURNAL   Biochemistry 40 (13), 3943-3950 (2001)
   PUBMED   11300774
REFERENCE   7  (bases 1 to 2916)
  AUTHORS   Smith,T.M., Lewis Carl,S.A. and Kirley,T.L.
  TITLE     Mutagenesis of two conserved tryptophan residues of the E-type
            ATPases: inactivation and conversion of an ecto-apyrase to an
            ecto-NTPase
  JOURNAL   Biochemistry 38 (18), 5849-5857 (1999)
   PUBMED   10231536
REFERENCE   8  (bases 1 to 2916)
  AUTHORS   Smith,T.M. and Kirley,T.L.
  TITLE     Cloning, sequencing, and expression of a human brain ecto-apyrase
            related to both the ecto-ATPases and CD39 ecto-apyrases1
  JOURNAL   Biochim Biophys Acta 1386 (1), 65-78 (1998)
   PUBMED   9675246
REFERENCE   9  (bases 1 to 2916)
  AUTHORS   Chadwick,B.P. and Frischauf,A.M.
  TITLE     The CD39-like gene family: identification of three new human
            members (CD39L2, CD39L3, and CD39L4), their murine homologues, and
            a member of the gene family from Drosophila melanogaster
  JOURNAL   Genomics 50 (3), 357-367 (1998)
   PUBMED   9676430
REFERENCE   10 (bases 1 to 2916)
  AUTHORS   Chadwick,B.P. and Frischauf,A.M.
  TITLE     Cloning and mapping of a human and mouse gene with homology to
            ecto-ATPase genes
  JOURNAL   Mamm Genome 8 (9), 668-672 (1997)
   PUBMED   9271669
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA209533.1, AF039917.1 and
            AC104186.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_001248.3.
            
            Summary: This gene encodes a plasma membrane-bound divalent
            cation-dependent E-type nucleotidase. The encoded protein is
            involved in the regulation of extracellular levels of ATP by
            hydrolysis of it and other nucleotides. Multiple transcript
            variants have been described. [provided by RefSeq, May 2014].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same protein (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.243051.1,
                                           SRR1803613.66938.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000301825.8/ ENSP00000301825.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                DA209533.1         29-56
            29-1576             AF039917.1         21-1568
            1577-2916           AC104186.2         136952-138291
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p22.1"
     gene            1..2916
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /note="ectonucleoside triphosphate diphosphohydrolase 3"
                     /db_xref="GeneID:956"
                     /db_xref="HGNC:HGNC:3365"
                     /db_xref="MIM:603161"
     exon            1..78
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /inference="alignment:Splign:2.1.0"
     exon            79..130
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..1680
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /EC_number="3.6.1.5"
                     /note="isoform 1 is encoded by transcript variant 2;
                     NTPDase 3; ecto-ATPase 3; ecto-ATPDase 3; ecto-apyrase 3;
                     CD39 antigen-like 3; ecto-ATP diphosphohydrolase 3"
                     /codon_start=1
                     /product="ectonucleoside triphosphate diphosphohydrolase 3
                     isoform 1"
                     /protein_id="NP_001239.2"
                     /db_xref="CCDS:CCDS2691.1"
                     /db_xref="GeneID:956"
                     /db_xref="HGNC:HGNC:3365"
                     /db_xref="MIM:603161"
                     /translation="MFTVLTRQPCEQAGLKALYRTPTIIALVVLLVSIVVLVSITVIQ
                     IHKQEVLPPGLKYGIVLDAGSSRTTVYVYQWPAEKENNTGVVSQTFKCSVKGSGISSY
                     GNNPQDVPRAFEECMQKVKGQVPSHLHGSTPIHLGATAGMRLLRLQNETAANEVLESI
                     QSYFKSQPFDFRGAQIISGQEEGVYGWITANYLMGNFLEKNLWHMWVHPHGVETTGAL
                     DLGGASTQISFVAGEKMDLNTSDIMQVSLYGYVYTLYTHSFQCYGRNEAEKKFLAMLL
                     QNSPTKNHLTNPCYPRDYSISFTMGHVFDSLCTVDQRPESYNPNDVITFEGTGDPSLC
                     KEKVASIFDFKACHDQETCSFDGVYQPKIKGPFVAFAGFYYTASALNLSGSFSLDTFN
                     SSTWNFCSQNWSQLPLLLPKFDEVYARSYCFSANYIYHLFVNGYKFTEETWPQIHFEK
                     EVGNSSIAWSLGYMLSLTNQIPAESPLIRLPIEPPVFVGTLAFFTAAALLCLAFLAYL
                     CSATRRKRHSEHAFDHAVDSD"
     exon            131..258
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /inference="alignment:Splign:2.1.0"
     exon            259..376
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /inference="alignment:Splign:2.1.0"
     exon            377..527
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /inference="alignment:Splign:2.1.0"
     exon            528..687
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /inference="alignment:Splign:2.1.0"
     exon            688..921
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /inference="alignment:Splign:2.1.0"
     exon            922..1194
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1305
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1443
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..2916
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2766..2771
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /note="hexamer: AATAAA"
     polyA_site      2798
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /note="major polyA site"
ORIGIN      
        1 gctgacacct ccttagcgct ggccgcgggc cgcctctgcg gcagcgctag tcgccttctc
       61 cgaatcggct ccgcacagct aggagaaaag atgttcactg tgctgacccg ccaaccatgt
      121 gagcaagcag gcctcaaggc cctctaccga actccaacca tcattgcctt ggtggtcttg
      181 cttgtgagta ttgtggtact tgtgagtatc actgtcatcc agatccacaa gcaagaggtc
      241 ctccctccag gactgaagta tggtattgtg ctggatgccg ggtcttcaag aaccacagtc
      301 tacgtgtatc aatggccagc agaaaaagag aataataccg gagtggtcag tcaaaccttc
      361 aaatgtagtg tgaaaggctc tggaatctcc agctatggaa ataaccccca agatgtcccc
      421 agagcctttg aggagtgtat gcaaaaagtc aaggggcagg ttccatccca cctccacgga
      481 tccaccccca ttcacctggg agccacggct gggatgcgct tgctgaggtt gcaaaatgaa
      541 acagcagcta atgaagtcct tgaaagcatc caaagctact tcaagtccca gccctttgac
      601 tttaggggtg ctcaaatcat ttctgggcaa gaagaagggg tatatggatg gattacagcc
      661 aactatttaa tgggaaattt cctggagaag aacctgtggc acatgtgggt gcacccgcat
      721 ggagtggaaa ccacgggtgc cctggactta ggtggtgcct ccacccaaat atccttcgtg
      781 gcaggagaga agatggatct gaacaccagc gacatcatgc aggtgtccct gtatggctac
      841 gtatacacgc tctacacaca cagcttccag tgctatggcc ggaatgaggc tgagaagaag
      901 tttctggcaa tgctcctgca gaattctcct accaaaaacc atctcaccaa tccctgttac
      961 cctcgggatt atagcatcag cttcaccatg ggccatgtat ttgatagcct gtgcactgtg
     1021 gaccagaggc cagaaagtta taaccccaat gatgtcatca cttttgaagg aactggggac
     1081 ccatctctgt gtaaggagaa ggtggcttcc atatttgact tcaaagcttg ccatgatcaa
     1141 gaaacctgtt cttttgatgg ggtttatcag ccaaagatta aagggccatt tgtggctttt
     1201 gcaggattct actacacagc cagtgcttta aatctttcag gtagcttttc cctggacacc
     1261 ttcaactcca gcacctggaa tttctgctca cagaattgga gtcagctccc actgctgctc
     1321 cccaaatttg atgaggtata tgcccgctct tactgcttct cagccaacta catctaccac
     1381 ttgtttgtga acggttacaa attcacagag gagacttggc cccaaataca ctttgaaaaa
     1441 gaagtgggga atagcagcat agcctggtct cttggctaca tgctcagcct gaccaaccag
     1501 atcccagctg aaagccctct gatccgtctg cccatagaac cacctgtctt tgtgggcacc
     1561 ctcgctttct tcacagcggc agccttgctg tgtctggcat ttcttgcata cctgtgttca
     1621 gcaaccagaa gaaagaggca ctccgagcat gcctttgacc atgcagtgga ttctgactga
     1681 gccttcaaag cagctcctgg agtccaatgg ctgcttagag tcagcctggg tggcaccagg
     1741 caatgcaggt gaagtggctg ccttcaggaa atacaactaa ctaaaatcaa acacctaggt
     1801 cacgtgcctc tcaaatactg atttctgcca cagcacctct tgaggcatcc cttggctatt
     1861 ctgtgcatat tgttcttcag agacctcact acccacatgc tgatctattg gggaacagag
     1921 aagagacagg ccacgaaggt caggctcttt atattaagtt ccccagagga agagtaagtt
     1981 gagaaggtat cagtttaatg ttgaagaatt gacctcaggg ctcagtttcc atttccctcc
     2041 ctcagtattc ttcctggcaa gatacccatt aagcatttcg ccaatcagaa tctcatttta
     2101 tagtttttcc cattggtctt taactaagac tttcttgtag caatctcgta agcagtgaac
     2161 cccctcagat cagtagaata tagtatctgg gggagaagac ttacttcctt cagggcagca
     2221 gccacagcca ggcttctgtc atacaggtag atcccgaagc acagagacat aaaaaaggtc
     2281 tcccagaaaa ctatagacca ttctccaagt ggaattccca cttagggctc tggtcactag
     2341 attgcaacct gtgtgtttgt catcatcctc atctcaccat tgtattgcta tgccctccca
     2401 taaaaacaca ttgatcccta gcaagattat tgcattccag attttactgc ctttgctagg
     2461 cttttgctta gcaaagggct gactttccat tgttatcatg gtgtatatat ttttgtcacc
     2521 attcccacaa gtatacttga tgttgtcata gaacgaacat cctactctat gatttactaa
     2581 ccaattactt tcccagatca tagacctctc tgcatagtag tcataggtct tgactttggg
     2641 gaaagaaaag gaagctgcag gaatatttat ctccaaagtc gaatgagaaa gaactccagc
     2701 aaatccaatg gctacaaact aaaaatcagc attatttcat attgctgttt cttagctgaa
     2761 tatggaataa agaactatta ttttattttg acttgcaagg ggtgtgtgtg tgttaacttc
     2821 catatggtac tctttctcct aaggacccta ggtgatattt atgagagaaa tttttttccc
     2881 tagcaaatta agaaacccat tcattaatat cacctt
//
