LOCUS       NP_001239                529 aa            linear   PRI 27-APR-2025
DEFINITION  ectonucleoside triphosphate diphosphohydrolase 3 isoform 1 [Homo
            sapiens].
ACCESSION   NP_001239
VERSION     NP_001239.2
DBSOURCE    REFSEQ: accession NM_001248.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 529)
  AUTHORS   Noronha-Matos,J.B., Pinto-Cardoso,R., Bessa-Andres,C.,
            Magalhaes-Cardoso,M.T., Ferreirinha,F., Costa,M.A., Marinhas,J.,
            Freitas,R., Lemos,R., Vilaca,A., Oliveira,A., Pelletier,J.,
            Sevigny,J. and Correia-de-Sa,P.
  TITLE     Silencing NTPDase3 activity rehabilitates the osteogenic commitment
            of post-menopausal stem cell bone progenitors
  JOURNAL   Stem Cell Res Ther 14 (1), 97 (2023)
   PUBMED   37076930
  REMARK    GeneRIF: Silencing NTPDase3 activity rehabilitates the osteogenic
            commitment of post-menopausal stem cell bone progenitors.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 529)
  AUTHORS   Docherty,F.M., Riemondy,K.A., Castro-Gutierrez,R., Dwulet,J.M.,
            Shilleh,A.H., Hansen,M.S., Williams,S.P.M., Armitage,L.H.,
            Santostefano,K.E., Wallet,M.A., Mathews,C.E., Triolo,T.M.,
            Benninger,R.K.P. and Russ,H.A.
  TITLE     ENTPD3 Marks Mature Stem Cell-Derived beta-Cells Formed by
            Self-Aggregation In Vitro
  JOURNAL   Diabetes 70 (11), 2554-2567 (2021)
   PUBMED   34380694
  REMARK    GeneRIF: ENTPD3 Marks Mature Stem Cell-Derived beta-Cells Formed by
            Self-Aggregation In Vitro.
REFERENCE   3  (residues 1 to 529)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (residues 1 to 529)
  AUTHORS   Feldbrugge,L., Moss,A.C., Yee,E.U., Csizmadia,E., Mitsuhashi,S.,
            Longhi,M.S., Sandhu,B., Stephan,H., Wu,Y., Cheifetz,A.S.,
            Muller,C.E., Sevigny,J., Robson,S.C. and Jiang,Z.G.
  TITLE     Expression of Ecto-nucleoside Triphosphate Diphosphohydrolases-2
            and -3 in the Enteric Nervous System Affects Inflammation in
            Experimental Colitis and Crohn's Disease
  JOURNAL   J Crohns Colitis 11 (9), 1113-1123 (2017)
   PUBMED   28472257
  REMARK    GeneRIF: NTPDase2 and -3 are ecto-enzymes expressed in the enteric
            nervous system. Both enzymes confer protection against gut
            inflammation in experimental colitis and exhibit alterations in
            Crohn's disease. These observations suggest that purinergic
            signalling modulated by E-NTPDases governs neuro-immune
            interactions that are relevant in Crohn's disease.
REFERENCE   5  (residues 1 to 529)
  AUTHORS   Roszek,K., Porowinska,D., Bajek,A., Holysz,M. and Czarnecka,J.
  TITLE     Chondrogenic Differentiation of Human Mesenchymal Stem Cells
            Results in Substantial Changes of Ecto-Nucleotides Metabolism
  JOURNAL   J Cell Biochem 116 (12), 2915-2923 (2015)
   PUBMED   26018728
  REMARK    GeneRIF: Despite the increased level of NTPDase1 and NTPDase3 mRNA
            expression in chondrogenically induced MSCs, their activity toward
            ATP remains quite low.
REFERENCE   6  (residues 1 to 529)
  AUTHORS   Yang,F., Hicks-Berger,C.A., Smith,T.M. and Kirley,T.L.
  TITLE     Site-directed mutagenesis of human nucleoside triphosphate
            diphosphohydrolase 3: the importance of residues in the apyrase
            conserved regions
  JOURNAL   Biochemistry 40 (13), 3943-3950 (2001)
   PUBMED   11300774
REFERENCE   7  (residues 1 to 529)
  AUTHORS   Smith,T.M., Lewis Carl,S.A. and Kirley,T.L.
  TITLE     Mutagenesis of two conserved tryptophan residues of the E-type
            ATPases: inactivation and conversion of an ecto-apyrase to an
            ecto-NTPase
  JOURNAL   Biochemistry 38 (18), 5849-5857 (1999)
   PUBMED   10231536
REFERENCE   8  (residues 1 to 529)
  AUTHORS   Smith,T.M. and Kirley,T.L.
  TITLE     Cloning, sequencing, and expression of a human brain ecto-apyrase
            related to both the ecto-ATPases and CD39 ecto-apyrases1
  JOURNAL   Biochim Biophys Acta 1386 (1), 65-78 (1998)
   PUBMED   9675246
REFERENCE   9  (residues 1 to 529)
  AUTHORS   Chadwick,B.P. and Frischauf,A.M.
  TITLE     The CD39-like gene family: identification of three new human
            members (CD39L2, CD39L3, and CD39L4), their murine homologues, and
            a member of the gene family from Drosophila melanogaster
  JOURNAL   Genomics 50 (3), 357-367 (1998)
   PUBMED   9676430
REFERENCE   10 (residues 1 to 529)
  AUTHORS   Chadwick,B.P. and Frischauf,A.M.
  TITLE     Cloning and mapping of a human and mouse gene with homology to
            ecto-ATPase genes
  JOURNAL   Mamm Genome 8 (9), 668-672 (1997)
   PUBMED   9271669
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA209533.1, AF039917.1 and
            AC104186.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jan 29, 2008 this sequence version replaced NP_001239.1.
            
            Summary: This gene encodes a plasma membrane-bound divalent
            cation-dependent E-type nucleotidase. The encoded protein is
            involved in the regulation of extracellular levels of ATP by
            hydrolysis of it and other nucleotides. Multiple transcript
            variants have been described. [provided by RefSeq, May 2014].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same protein (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.243051.1,
                                           SRR1803613.66938.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000301825.8/ ENSP00000301825.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p22.1"
     Protein         1..529
                     /product="ectonucleoside triphosphate diphosphohydrolase 3
                     isoform 1"
                     /EC_number="3.6.1.5"
                     /note="NTPDase 3; ecto-ATPase 3; ecto-ATPDase 3;
                     ecto-apyrase 3; CD39 antigen-like 3; ecto-ATP
                     diphosphohydrolase 3"
                     /calculated_mol_wt=58974
     Site            23..43
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O75355.2)"
     Region          57..468
                     /region_name="ASKHA_NBD_NTPDase3"
                     /note="nucleotide-binding domain (NBD) of ectonucleoside
                     triphosphate diphosphohydrolase 3 (NTPDase3) and similar
                     proteins; cd24112"
                     /db_xref="CDD:466962"
     Site            81
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O75355.2)"
     Site            149
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O75355.2)"
     Site            238
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O75355.2)"
     Site            381
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O75355.2)"
     Site            392
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O75355.2)"
     Site            402
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O75355.2)"
     Site            454
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O75355.2)"
     Site            486..506
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O75355.2)"
     CDS             1..529
                     /gene="ENTPD3"
                     /gene_synonym="CD39L3; HB6; NTPDase-3"
                     /coded_by="NM_001248.4:91..1680"
                     /note="isoform 1 is encoded by transcript variant 2"
                     /db_xref="CCDS:CCDS2691.1"
                     /db_xref="GeneID:956"
                     /db_xref="HGNC:HGNC:3365"
                     /db_xref="MIM:603161"
ORIGIN      
        1 mftvltrqpc eqaglkalyr tptiialvvl lvsivvlvsi tviqihkqev lppglkygiv
       61 ldagssrttv yvyqwpaeke nntgvvsqtf kcsvkgsgis sygnnpqdvp rafeecmqkv
      121 kgqvpshlhg stpihlgata gmrllrlqne taanevlesi qsyfksqpfd frgaqiisgq
      181 eegvygwita nylmgnflek nlwhmwvhph gvettgaldl ggastqisfv agekmdlnts
      241 dimqvslygy vytlythsfq cygrneaekk flamllqnsp tknhltnpcy prdysisftm
      301 ghvfdslctv dqrpesynpn dvitfegtgd pslckekvas ifdfkachdq etcsfdgvyq
      361 pkikgpfvaf agfyytasal nlsgsfsldt fnsstwnfcs qnwsqlplll pkfdevyars
      421 ycfsanyiyh lfvngykfte etwpqihfek evgnssiaws lgymlsltnq ipaesplirl
      481 pieppvfvgt lafftaaall claflaylcs atrrkrhseh afdhavdsd
//
