LOCUS       NP_982293                495 aa            linear   PRI 27-APR-2025
DEFINITION  ectonucleoside triphosphate diphosphohydrolase 2 isoform 1 [Homo
            sapiens].
ACCESSION   NP_982293
VERSION     NP_982293.1
DBSOURCE    REFSEQ: accession NM_203468.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 495)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (residues 1 to 495)
  AUTHORS   Villamonte,M.L., Torrejon-Escribano,B., Rodriguez-Martinez,A.,
            Trapero,C., Vidal,A., Gomez de Aranda,I., Sevigny,J.,
            Matias-Guiu,X. and Martin-Satue,M.
  TITLE     Characterization of ecto-nucleotidases in human oviducts with an
            improved approach simultaneously identifying protein expression and
            in situ enzyme activity
  JOURNAL   Histochem Cell Biol 149 (3), 269-276 (2018)
   PUBMED   29273916
  REMARK    GeneRIF: In oviducts, remarkably, ectonucleoside triphosphate
            diphosphohydrolase 2 (NTPDase2), with the ability to hydrolyze ATP
            to AMP, are expressed in ciliated epithelial cells but with
            different subcellular localization.
REFERENCE   3  (residues 1 to 495)
  AUTHORS   Chiu,D.K., Tse,A.P., Xu,I.M., Di Cui,J., Lai,R.K., Li,L.L.,
            Koh,H.Y., Tsang,F.H., Wei,L.L., Wong,C.M., Ng,I.O. and Wong,C.C.
  TITLE     Hypoxia inducible factor HIF-1 promotes myeloid-derived suppressor
            cells accumulation through ENTPD2/CD39L1 in hepatocellular
            carcinoma
  JOURNAL   Nat Commun 8 (1), 517 (2017)
   PUBMED   28894087
  REMARK    GeneRIF: Hypoxia upregulates ENTPD2 through HIF-1alpha in
            hepatocellular carcinoma and subsequently leading to the
            accumulation of extracellular 5'-AMP, which maintains
            myeloid-derived suppressor cells undifferentiated.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 495)
  AUTHORS   Feldbrugge,L., Moss,A.C., Yee,E.U., Csizmadia,E., Mitsuhashi,S.,
            Longhi,M.S., Sandhu,B., Stephan,H., Wu,Y., Cheifetz,A.S.,
            Muller,C.E., Sevigny,J., Robson,S.C. and Jiang,Z.G.
  TITLE     Expression of Ecto-nucleoside Triphosphate Diphosphohydrolases-2
            and -3 in the Enteric Nervous System Affects Inflammation in
            Experimental Colitis and Crohn's Disease
  JOURNAL   J Crohns Colitis 11 (9), 1113-1123 (2017)
   PUBMED   28472257
  REMARK    GeneRIF: NTPDase2 and -3 are ecto-enzymes expressed in the enteric
            nervous system. Both enzymes confer protection against gut
            inflammation in experimental colitis and exhibit alterations in
            Crohn's disease. These observations suggest that purinergic
            signalling modulated by E-NTPDases governs neuro-immune
            interactions that are relevant in Crohn's disease.
REFERENCE   5  (residues 1 to 495)
  AUTHORS   Chiang,W.C. and Knowles,A.F.
  TITLE     Inhibition of human NTPDase 2 by modification of an intramembrane
            cysteine by p-chloromercuriphenylsulfonate and oxidative
            cross-linking of the transmembrane domains
  JOURNAL   Biochemistry 47 (33), 8775-8785 (2008)
   PUBMED   18656957
  REMARK    GeneRIF: The role of cysteine 26 in the catalytic activity of
            NTPDase-2 and as the target of p-chloromercuriphenylsulfonate
            (pCMPS), a sulfhydryl reagent and NTPDase-2 inhibitor, is reported.
REFERENCE   6  (residues 1 to 495)
  AUTHORS   Mateo,J., Kreda,S., Henry,C.E., Harden,T.K. and Boyer,J.L.
  TITLE     Requirement of Cys399 for processing of the human ecto-ATPase
            (NTPDase2) and its implications for determination of the activities
            of splice variants of the enzyme
  JOURNAL   J Biol Chem 278 (41), 39960-39968 (2003)
   PUBMED   12888562
  REMARK    GeneRIF: the lack of ecto-nucleotidase activity exhibited by
            NTPDase2 beta and -2 gamma and the C399S mutant, as well as the
            large reduction of activity in the N443D mutant are due to
            alterations in the folding/maturation of these proteins
REFERENCE   7  (residues 1 to 495)
  AUTHORS   Grinthal,A. and Guidotti,G.
  TITLE     Transmembrane domains confer different substrate specificities and
            adenosine diphosphate hydrolysis mechanisms on CD39, CD39L1, and
            chimeras
  JOURNAL   Biochemistry 41 (6), 1947-1956 (2002)
   PUBMED   11827541
REFERENCE   8  (residues 1 to 495)
  AUTHORS   Suzuki,H., Fukunishi,Y., Kagawa,I., Saito,R., Oda,H., Endo,T.,
            Kondo,S., Bono,H., Okazaki,Y. and Hayashizaki,Y.
  TITLE     Protein-protein interaction panel using mouse full-length cDNAs
  JOURNAL   Genome Res 11 (10), 1758-1765 (2001)
   PUBMED   11591653
REFERENCE   9  (residues 1 to 495)
  AUTHORS   Mateo,J., Harden,T.K. and Boyer,J.L.
  TITLE     Functional expression of a cDNA encoding a human ecto-ATPase
  JOURNAL   Br J Pharmacol 128 (2), 396-402 (1999)
   PUBMED   10510450
REFERENCE   10 (residues 1 to 495)
  AUTHORS   Chadwick,B.P. and Frischauf,A.M.
  TITLE     Cloning and mapping of a human and mouse gene with homology to
            ecto-ATPase genes
  JOURNAL   Mamm Genome 8 (9), 668-672 (1997)
   PUBMED   9271669
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL807752.10, CB045339.1,
            AF144748.1 and BC035738.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: The protein encoded by this gene is the type 2 enzyme of
            the ecto-nucleoside triphosphate diphosphohydrolase family
            (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that
            hydrolyze 5'-triphosphates. This ecto-ATPase is an integral
            membrane protein. Alternative splicing of this gene results in
            multiple transcript variants. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1078965.1,
                                           SRR3476690.533940.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1966682,
                                           SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000355097.7/ ENSP00000347213.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.3"
     Protein         1..495
                     /product="ectonucleoside triphosphate diphosphohydrolase 2
                     isoform 1"
                     /EC_number="3.6.1.-"
                     /note="NTPDase 2; ecto-ATPase 2; ecto-ATPDase 2; CD39
                     antigen-like 1; ecto-ATP diphosphohydrolase 2"
                     /calculated_mol_wt=53535
     Site            8..28
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y5L3.1)"
     Region          37..449
                     /region_name="ASKHA_NBD_NTPDase2"
                     /note="nucleotide-binding domain (NBD) of ectonucleoside
                     triphosphate diphosphohydrolase 2 (NTPDase2) and similar
                     proteins; cd24111"
                     /db_xref="CDD:466961"
     Site            64
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9Y5L3.1)"
     Site            129
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9Y5L3.1)"
     Site            294
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9Y5L3.1)"
     Site            378
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9Y5L3.1)"
     Site            443
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:12888562; propagated from
                     UniProtKB/Swiss-Prot (Q9Y5L3.1)"
     Site            463..483
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y5L3.1)"
     CDS             1..495
                     /gene="ENTPD2"
                     /gene_synonym="CD39L1; NTPDase-2"
                     /coded_by="NM_203468.3:65..1552"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS7026.1"
                     /db_xref="GeneID:954"
                     /db_xref="HGNC:HGNC:3364"
                     /db_xref="MIM:602012"
ORIGIN      
        1 magkvrsllp plllaaagla gllllcvptr dvreppalky givldagssh tsmfiykwpa
       61 dkendtgivg qhsscdvpgg gissyadnps gasqslvgcl eqalqdvpke rhagtplylg
      121 atagmrllnl tnpeastsvl mavthtltqy pfdfrgaril sgqeegvfgw vtanyllenf
      181 ikygwvgrwf rprkgtlgam dlggastqit fettspaedr asevqlhlyg qhyrvythsf
      241 lcygrdqvlq rllasalqth gfhpcwprgf stqvllgdvy qspctmaqrp qnfnssarvs
      301 lsgssdphlc rdlvsglfsf sscpfsrcsf ngvfqppvag nfvafsaffy tvdflrtsmg
      361 lpvatlqqle aaavnvcnqt waqlqarvpg qrarladyca gamfvqqlls rgygfderaf
      421 ggvifqkkaa dtavgwalgy mlnltnlipa dppglrkgtd fsswvvllll fasallaalv
      481 lllrqvhsak lpsti
//
