LOCUS       NM_001977               6861 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens glutamyl aminopeptidase (ENPEP), transcript variant 1,
            mRNA.
ACCESSION   NM_001977
VERSION     NM_001977.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Huang,W., Chen,A.C.H., Wei,X., Fong,S.W., Yeung,W.S.B. and Lee,Y.L.
  TITLE     Uncovering the role of TET2-mediated ENPEP activation in
            trophoblast cell fate determination
  JOURNAL   Cell Mol Life Sci 81 (1), 270 (2024)
   PUBMED   38886218
  REMARK    GeneRIF: Uncovering the role of TET2-mediated ENPEP activation in
            trophoblast cell fate determination.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Valverde,A., Dunys,J., Lorivel,T., Debayle,D., Gay,A.S.,
            Lacas-Gervais,S., Roques,B.P., Chami,M. and Checler,F.
  TITLE     Aminopeptidase A contributes to biochemical, anatomical and
            cognitive defects in Alzheimer's disease (AD) mouse model and is
            increased at early stage in sporadic AD brain
  JOURNAL   Acta Neuropathol 141 (6), 823-839 (2021)
   PUBMED   33881611
  REMARK    GeneRIF: Aminopeptidase A contributes to biochemical, anatomical
            and cognitive defects in Alzheimer's disease (AD) mouse model and
            is increased at early stage in sporadic AD brain.
REFERENCE   3  (bases 1 to 6861)
  AUTHORS   Aung,M., Konoshita,T., Moodley,J., Naicker,T., Connolly,C.,
            Khaliq,O.P. and Gathiram,P.
  TITLE     Aminopeptidase A (ENPEP) gene polymorphisms and preeclampsia:
            Descriptive analysis
  JOURNAL   Eur J Obstet Gynecol Reprod Biol 258, 70-74 (2021)
   PUBMED   33421813
  REMARK    GeneRIF: Aminopeptidase A (ENPEP) gene polymorphisms and
            preeclampsia: Descriptive analysis.
REFERENCE   4  (bases 1 to 6861)
  AUTHORS   Qi,F., Qian,S., Zhang,S. and Zhang,Z.
  TITLE     Single cell RNA sequencing of 13 human tissues identify cell types
            and receptors of human coronaviruses
  JOURNAL   Biochem Biophys Res Commun 526 (1), 135-140 (2020)
   PUBMED   32199615
REFERENCE   5  (bases 1 to 6861)
  AUTHORS   Chuang,H.Y., Jiang,J.K., Yang,M.H., Wang,H.W., Li,M.C., Tsai,C.Y.,
            Jhang,Y.Y. and Huang,J.C.
  TITLE     Aminopeptidase A initiates tumorigenesis and enhances tumor cell
            stemness via TWIST1 upregulation in colorectal cancer
  JOURNAL   Oncotarget 8 (13), 21266-21280 (2017)
   PUBMED   28177885
  REMARK    GeneRIF: We suggest that APA enzymatic activity affects tumor
            initiation and cancer malignancy in a TWIST-dependent manner
REFERENCE   6  (bases 1 to 6861)
  AUTHORS   Malinauskas,M., Wallenius,V., Fandriks,L. and Casselbrant,A.
  TITLE     Local expression of AP/AngIV/IRAP and effect of AngIV on
            glucose-induced epithelial transport in human jejunal mucosa
  JOURNAL   J Renin Angiotensin Aldosterone Syst 16 (4), 1101-1108 (2015)
   PUBMED   26311161
  REMARK    GeneRIF: The substrate Angiotensin II, the enzymes
            aminopeptidases-A, B, M as well as IRAP were detected in the
            jejunal mucosa.
REFERENCE   7  (bases 1 to 6861)
  AUTHORS   Li,L., Wang,J. and Cooper,M.D.
  TITLE     cDNA cloning and expression of human glutamyl aminopeptidase
            (aminopeptidase A)
  JOURNAL   Genomics 17 (3), 657-664 (1993)
   PUBMED   8244382
REFERENCE   8  (bases 1 to 6861)
  AUTHORS   Nanus,D.M., Engelstein,D., Gastl,G.A., Gluck,L., Vidal,M.J.,
            Morrison,M., Finstad,C.L., Bander,N.H. and Albino,A.P.
  TITLE     Molecular cloning of the human kidney differentiation antigen
            gp160: human aminopeptidase A
  JOURNAL   Proc Natl Acad Sci U S A 90 (15), 7069-7073 (1993)
   PUBMED   8346219
REFERENCE   9  (bases 1 to 6861)
  AUTHORS   Stefanovic,V., Vlahovic,P., Ardaillou,N. and Ardaillou,R.
  TITLE     Receptor-mediated induction of aminopeptidase A (APA) of human
            glomerular epithelial cells (HGEC) by glucocorticoids
  JOURNAL   FEBS Lett 294 (3), 171-174 (1991)
   PUBMED   1756856
REFERENCE   10 (bases 1 to 6861)
  AUTHORS   Nanus,D.M., Pfeffer,L.M., Bander,N.H., Bahri,S. and Albino,A.P.
  TITLE     Antiproliferative and antitumor effects of alpha-interferon in
            renal cell carcinomas: correlation with the expression of a
            kidney-associated differentiation glycoprotein
  JOURNAL   Cancer Res 50 (14), 4190-4194 (1990)
   PUBMED   2364374
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC094770.1, AC098798.4,
            BG400415.1, AL568777.3, AI640493.1 and AC017068.7.
            
            On Nov 23, 2018 this sequence version replaced NM_001977.3.
            
            Summary: The ENPEP gene encodes glutamyl aminopeptidase, a type II
            integral membrane protein with an extracellular zinc-binding
            domain. This protein can upregulate blood pressure by cleaving the
            N-terminal aspartate from angiotensin II, and can regulate blood
            vessel formation and enhance tumorigenesis in some tissues. Along
            with ANPEP and DPP4, ENPEP was found to be a candidate co-receptor
            for the coronavirus SARS-CoV-2, which causes COVID-19. [provided by
            RefSeq, Apr 2020].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AM888273.1, AM888274.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265162.10/ ENSP00000265162.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-897               BC094770.1         64-960
            898-898             AC098798.4         31656-31656
            899-3781            BC094770.1         962-3844
            3782-4014           BG400415.1         405-637
            4015-4575           AL568777.3         313-873             c
            4576-4913           AI640493.1         10-347              c
            4914-6861           AC017068.7         43497-45444
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q25"
     gene            1..6861
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /note="glutamyl aminopeptidase"
                     /db_xref="GeneID:2028"
                     /db_xref="HGNC:HGNC:3355"
                     /db_xref="MIM:138297"
     exon            1..904
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     CDS             261..3134
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /EC_number="3.4.11.7"
                     /note="isoform 1 is encoded by transcript variant 1; EAP;
                     AP-A; aminopeptidase A; differentiation antigen gp160"
                     /codon_start=1
                     /product="glutamyl aminopeptidase isoform 1"
                     /protein_id="NP_001968.3"
                     /db_xref="CCDS:CCDS3691.1"
                     /db_xref="GeneID:2028"
                     /db_xref="HGNC:HGNC:3355"
                     /db_xref="MIM:138297"
                     /translation="MNFAEREGSKRYCIQTKHVAILCAVVVGVGLIVGLAVGLTRSCD
                     SSGDGGPGTAPAPSHLPSSTASPSGPPAQDQDICPASEDESGQWKNFRLPDFVNPVHY
                     DLHVKPLLEEDTYTGTVSISINLSAPTRYLWLHLRETRITRLPELKRPSGDQVQVRRC
                     FEYKKQEYVVVEAEEELTPSSGDGLYLLTMEFAGWLNGSLVGFYRTTYTENGQVKSIV
                     ATDHEPTDARKSFPCFDEPNKKATYTISITHPKEYGALSNMPVAKEESVDDKWTRTTF
                     EKSVPMSTYLVCFAVHQFDSVKRISNSGKPLTIYVQPEQKHTAEYAANITKSVFDYFE
                     EYFAMNYSLPKLDKIAIPDFGTGAMENWGLITYRETNLLYDPKESASSNQQRVATVVA
                     HELVHQWFGNIVTMDWWEDLWLNEGFASFFEFLGVNHAETDWQMRDQMLLEDVLPVQE
                     DDSLMSSHPIIVTVTTPDEITSVFDGISYSKGSSILRMLEDWIKPENFQKGCQMYLEK
                     YQFKNAKTSDFWAALEEASRLPVKEVMDTWTRQMGYPVLNVNGVKNITQKRFLLDPRA
                     NPSQPPSDLGYTWNIPVKWTEDNITSSVLFNRSEKEGITLNSSNPSGNAFLKINPDHI
                     GFYRVNYEVATWDSIATALSLNHKTFSSADRASLIDDAFALARAQLLDYKVALNLTKY
                     LKREENFLPWQRVISAVTYIISMFEDDKELYPMIEEYFQGQVKPIADSLGWNDAGDHV
                     TKLLRSSVLGFACKMGDREALNNASSLFEQWLNGTVSLPVNLRLLVYRYGMQNSGNEI
                     SWNYTLEQYQKTSLAQEKEKLLYGLASVKNVTLLSRYLDLLKDTNLIKTQDVFTVIRY
                     ISYNSYGKNMAWNWIQLNWDYLVNRYTLNNRNLGRIVTIAEPFNTELQLWQMESFFAK
                     YPQAGAGEKPREQVLETVKNNIEWLKQHRNTIREWFFNLLESG"
     exon            905..1046
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1178
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1299
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1454
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1568
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1703
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1769
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            1770..1835
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1987
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            1988..2067
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            2068..2204
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2260
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            2261..2411
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            2412..2485
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            2486..2590
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            2591..2761
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            2762..2902
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            2903..2981
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     exon            2982..6861
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3761..3766
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /note="hexamer: AATAAA"
     polyA_site      3788
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /note="major polyA site"
     regulatory      3819..3824
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /note="hexamer: AATAAA"
     polyA_site      3846
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
     regulatory      4886..4891
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /note="hexamer: AATAAA"
     polyA_site      4913
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
     regulatory      4969..4974
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /note="hexamer: ATTAAA"
     regulatory      4981..4986
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /note="hexamer: AACAAA"
     polyA_site      4986
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
     polyA_site      5008
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
     regulatory      6840..6845
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
                     /note="hexamer: AATAAA"
     polyA_site      6861
                     /gene="ENPEP"
                     /gene_synonym="APA; CD249; gp160"
ORIGIN      
        1 gccagccctc ttcctcccac tcggctcctc ttacggagtc ctcattccac cccccttgtt
       61 tccgcattca tcctgagtgg ctggtgggaa cgtgaaaagg gagaggaaaa ggcgcaagaa
      121 gccagagaga ggggtgtggg aaaagcgaaa acaggctgcc aaatcagggg attccttcca
      181 atttaaaaag gaagtctgct gacgttagtt agttaaattt aacatctttt tatgtgtaac
      241 acttgacttt ggaagcaaaa atgaactttg cggagagaga gggctctaag agatactgca
      301 ttcaaacgaa acatgtggcc attctctgtg cggtggtggt gggtgtagga ttaatagtgg
      361 gacttgccgt gggcttgacc agatcgtgtg actccagcgg ggacggcggg ccgggcactg
      421 cgccagctcc ttcccacctg ccttcttcca cggccagccc ctcaggtcct cctgcccagg
      481 accaggacat ctgcccggcc agtgaggatg agagcggaca gtggaaaaac tttcgactgc
      541 cggacttcgt caacccagtc cactacgacc tgcacgtgaa gcccctgttg gaggaggaca
      601 cctacacggg caccgtgagc atctccatca acctgagcgc tcccacccgg tacctgtggc
      661 tgcacctccg ggagaccagg atcacccggc tcccggagct gaagaggccc tctggggacc
      721 aggtgcaagt ccggaggtgt ttcgagtaca aaaagcagga gtacgtggtg gtcgaggcgg
      781 aggaagagct tacccccagc agtggagatg gcctgtatct cctgaccatg gagttcgccg
      841 gctggctgaa cggctccctc gtgggatttt atagaaccac ctacacggag aacggacaag
      901 tcaagagcat agtggccacc gatcatgaac caacagatgc caggaaatct tttccttgtt
      961 ttgatgagcc caacaaaaag gcaacttata caatatctat cacccatccc aaagaatacg
     1021 gagcactttc aaatatgcca gtggcgaaag aagagtcagt ggatgataaa tggactcgaa
     1081 caacttttga gaagtctgtc cccatgagca cgtacctggt gtgctttgct gtacatcaat
     1141 ttgactctgt aaagagaata tcaaatagtg gaaaacctct tacaatttat gtccagccag
     1201 agcaaaagca cacagccgaa tatgctgcaa acataactaa aagtgtgttt gattattttg
     1261 aagaatactt tgctatgaat tattctcttc ctaaattaga taaaatcgct attccagatt
     1321 ttggcactgg tgccatggag aactggggac tcatcacgta cagagaaacg aacctgcttt
     1381 atgaccctaa ggaatcagcc tcatcaaacc aacagagggt ggccactgtg gttgcccatg
     1441 aacttgtgca tcagtggttt ggaaatattg tgaccatgga ctggtgggaa gacttgtggc
     1501 taaatgaagg atttgcttct ttctttgagt ttctgggagt aaaccatgca gaaacagact
     1561 ggcaaatgcg tgaccaaatg ttacttgaag atgtattacc tgttcaagag gatgattctt
     1621 tgatgtcttc gcatccaatt attgtgactg tgacaacccc tgatgaaata acatctgttt
     1681 ttgatggaat atcctatagc aagggatctt ctattttgag aatgcttgaa gactggataa
     1741 aaccagagaa ttttcaaaaa ggatgtcaga tgtacttgga aaaataccaa ttcaagaatg
     1801 caaaaacttc tgatttttgg gcagcactgg aagaggcaag taggctacca gtgaaagaag
     1861 taatggacac ctggaccaga cagatgggtt atcctgtgct taacgtgaac ggtgtcaaga
     1921 acatcacaca gaaacgcttt ttgttggacc caagagctaa cccttctcag cccccttcag
     1981 atcttggtta tacatggaat atcccagtta aatggactga agataatata acaagcagtg
     2041 tgttatttaa taggtcagaa aaagaaggaa tcactttgaa ctcctctaat cctagtggaa
     2101 atgcttttct caaaataaac ccagatcata ttgggtttta tcgtgtaaat tatgaagtag
     2161 caacttggga ctcgatagct acagcgctct ccttgaacca caagacattt tcttcagcag
     2221 atcgtgcaag tcttattgat gatgcttttg ccttggcaag agctcaactt ctagattata
     2281 aggtggcttt gaacttgacc aagtatctca aaagggaaga gaatttttta ccatggcaga
     2341 gagtaatttc agctgtaacc tacatcatta gcatgtttga agatgataaa gagctatatc
     2401 ctatgattga ggaatacttc caaggtcaag tgaagcctat tgcagattct ctgggatgga
     2461 atgatgctgg agaccatgtc acaaagttac tccgttcctc cgtgttaggg tttgcgtgca
     2521 agatgggaga cagagaagcc ttgaacaatg cttcctcgtt atttgagcag tggctaaatg
     2581 ggactgtaag ccttcccgta aatctcaggc ttctggtgta tcggtatggg atgcagaact
     2641 ctggcaatga gatttcatgg aactacactc ttgagcaata ccagaaaact tcattagctc
     2701 aagaaaaaga aaaactgctg tatggattag catcagtgaa gaacgttact cttttgtcaa
     2761 ggtatttgga tttgctcaag gacacgaacc ttattaaaac tcaggatgtg tttacagtca
     2821 ttcgatatat ctcatataac agctatggga agaacatggc ctggaattgg atacaactca
     2881 actgggacta tctagtcaac agatatacac tcaataacag aaaccttggc cgaattgtca
     2941 caatagcaga gccattcaac actgaactgc aactgtggca gatggagagc ttttttgcaa
     3001 aatatccaca agctggagca ggagaaaaac ctagggaaca agtgctggaa acagtgaaaa
     3061 acaatataga gtggctaaaa caacatagaa acaccatcag agaatggttt tttaatttac
     3121 ttgagagtgg ttaatgtatt caaatgttag agtttaattt tgtgaatcta ttgtttctcc
     3181 tctgaagcat ttggtggcct aatttacaag cacgatggag agagccttat aaacagataa
     3241 tgcttttact aagcactgtg tttatatgtc ttgcaaagcc tttaaattgt tcctctttgt
     3301 ttatgaagaa agatactaat agagtactta atatactcag ggattccttc taagtgtact
     3361 tcataagata ttctttacaa actgaagata atgaaatagt tattttaata cctttaaata
     3421 tcattctttg tatcttaaat ctgtgaagta gaacaatttg gtcttagctt tacattttca
     3481 gtaccaaaga aacaccactt aatcttctct cttgattgat ttttaaagtt taaataccag
     3541 tacttgaggg accaatatta ccatcttaat ctttatttta ttattcagaa ttatacagac
     3601 ctaatatcat tttattcaca tatgtcttac tactttaaat cctaccaaag taacctgggc
     3661 ttaagtttta cttgaggatc atatgaatta gccaaaagaa tggctgactg ttgcctttgc
     3721 tcttataaaa tcaataagag aactctcctc tttttttaaa aataaatcta aatgattatc
     3781 ttgaaaaagt attgttttcc agtgtcactt gactcttgaa taaaaataac tgcagtgttc
     3841 cccaaataat ttaacacatt gcaaatatga ttcaagaatc tggccttttt catgcttggt
     3901 actctcttct cttgctccat tccttacaaa gctccttcaa atatgctatg aaattagagg
     3961 gaacatagtg cttaactctg ctccatggat tttagaggaa aaaagctaaa taattggaaa
     4021 ctgttcagga aagagagaag gcagattatt tcttaaaact aaatggaatt agaatttagc
     4081 tttgacctca cctagcaatc taagtgtgaa aatggggaga cctgtgggca tccccagcat
     4141 gactctgcat atctgagcat atatctcctt atagatcaat tttatgtata aacccttaga
     4201 tcctaatcca tacagaatag cactgatcaa atttaaatgc atgacttgat taaagacatc
     4261 aatttcatag aagctggtgg caagaatatg tttaatatgg cacttgatac ctcctagatc
     4321 cataatgtaa gtccaatagg cagaaaagat ggtaagttac tatgtttcaa tctgctatat
     4381 ctagaaaatc taaatttatt tggaactttc aaaaatgtaa ggtaatttag aatcggcttg
     4441 atgagaattg aagcatatgg aaacaaaacc ttagctttaa ctttatttat acaataatgt
     4501 gccctaatca tgtactctga aactttataa actttcctgt tgctgtttct taattatcac
     4561 ttctcaatcc gtacagccaa actagtaaat tcaaatgtag actgggttgt gggtggcaga
     4621 tatagaagaa gaaatttcca gaccaaaaaa tattaaatat tattttaaat tatttcagaa
     4681 gcaaaaacat attaacatca taacttacca gaaaaacccc actatcctca taagattaac
     4741 ttaaaattct gaccacaatc agaaccagca cttgaagtct atgtattgtt tcaagtagga
     4801 catattatga ggtgagatgg tttatccttc tgttttatga aattaataca ttttaaaaga
     4861 aaaatgttag tttaaggggg tggtaaataa aacagatttc aacttgccta caagacattc
     4921 tatttcatgc cttttccttt tgttgttcta tttcattcta ttttaagcat taaaactgag
     4981 aacaaattgt ttacttcttt tcactccaca cgcgaaagag ttgtatgtac tttgtcagaa
     5041 taagtcttct agaccatcag gtccttctca aacaacacct gttttttaaa aggctgatgc
     5101 ctaatagata gatctatgcc aagcacagtt tattactttg cttgcttggt tttgcacagt
     5161 attcacaggg gacaaaaaat ttaatatgat gtttccaaaa ttaagccaca gtaaatttaa
     5221 ataattaatt ttaattgaaa aaaagtaacc atttaatttc aagaattaac tggaaaatat
     5281 gggaagcaag gtcatgtagc aaaaagcaag gccagtatta ctgcctcccc tctaggcagc
     5341 ccactcctgt attccagatc attgctatgt acccacataa ttataaatta ggagtttaaa
     5401 taataaaaca tagacgtaaa actgacaggc aagggaggtt tgggaaacag gggtgagatg
     5461 tatacctggg ttgaaggtaa tgatactcct acagcttacc ctccaagttg gtgaagtgtt
     5521 agtccaaaag tagaaacaac agagtagggg caaaaatcta gaacaataat tctcaaaggg
     5581 aattggggga tgagaaggaa gtagaaaaga ggggattcaa attaccagtg gtgctttcta
     5641 aaacacggtt aggtaatcat catcatcaga ctttcctttt tttttcaagc atttttataa
     5701 acactagcac tccacataac attaattttt acaaaacccc cttaaagtag atactgttat
     5761 tgttcttatt ttagagtgga taaattgaga tggagaagtt atagaacttg cacaaggcta
     5821 caaaagttgt atttggcacc agccagtatt tgaacccagg cagtttatca ctctaatcta
     5881 cattttaatc tgtattagag taaaaatatg cttttgaaat cagagtgacc tgggttggaa
     5941 tatcaactac ttggtagttg agtgctttta acctctccat tgttgtttca tctataaagt
     6001 aaggatcaaa atagtagcta cctcaatggt agttgtgagg attaaataca aaaatgcatg
     6061 taaaggacat acgtatagga aataacaatc actcaataag tgttagctct tattattgtt
     6121 actattatat ctccccatcc ctacttaaga cactagtttg aagaataaaa taaactttgg
     6181 ttcaggaata accaaaccta agcaactgaa ggcactaagg gtaggctatg tgttagcctg
     6241 tgttattgtt cataaatagt aattgccctg gtctggaaga ggattctata tcctacccca
     6301 ttgtagtcta gcatggtatg tgacttgctt tggccagtga aacagagtga aaatgatgta
     6361 tgtcaatttt aggcagaaac gtccagagcc agcacttggt ttgccatgtt ctccgtgttt
     6421 cttctgctcc tagatgcagc agtattccag atagaggccg cactatgagc ctgaattcca
     6481 ggataaaaac aacatggaat agaacttagc cagcacagag tacacatgtg ctgtaaatga
     6541 gaaatacccc tttgttattg taaatcactg agattttgag gttgtctgtt actgcagcat
     6601 aatctagcct aatataaaca ccaaactaat atactcctct accatagtaa tttatttctt
     6661 gttctttctc ctcctccttc ctctacttca gggtttagcg accttttgcc tgcctgtttt
     6721 tgtaaggcct gaaagctaaa catagtttta catttttaga tggttgaaaa aatcaaaagg
     6781 agaataatag ttcatgatgt aaaaattata taaaatttaa ataaatgtcg gcatccttaa
     6841 ataaagtttt attggaacac a
//
