LOCUS       NM_006375               5126 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens ecto-NOX disulfide-thiol exchanger 2 (ENOX2),
            transcript variant 1, mRNA.
ACCESSION   NM_006375
VERSION     NM_006375.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Kam,N.W., Laczka,O., Li,X., Wilkinson,J., Hung,D., Lai,S.P.H.,
            Wu,K.C., Tsao,S.W., Dai,W., Che,C.M., Lee,V.H. and Kwong,D.L.
  TITLE     ENOX2 inhibition enhances infiltration of effector memory T-cell
            and mediates response to chemotherapy in immune-quiescent
            nasopharyngeal carcinoma
  JOURNAL   J Adv Res 56, 69-86 (2024)
   PUBMED   37061217
  REMARK    GeneRIF: ENOX2 inhibition enhances infiltration of effector memory
            T-cell and mediates response to chemotherapy in immune-quiescent
            nasopharyngeal carcinoma.
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Baykal-Kose,S., Voldoire,M., Desterke,C., Sorel,N., Cayssials,E.,
            Johnson-Ansah,H., Guerci-Bresler,A., Bennaceur-Griscelli,A.,
            Chomel,J.C. and Turhan,A.G.
  TITLE     ENOX2 NADH Oxidase: A BCR-ABL1-Dependent Cell Surface and Secreted
            Redox Protein in Chronic Myeloid Leukemia
  JOURNAL   Turk J Haematol 40 (2), 101-117 (2023)
   PUBMED   37026766
  REMARK    GeneRIF: ENOX2 NADH Oxidase: A BCR-ABL1-Dependent Cell Surface and
            Secreted Redox Protein in Chronic Myeloid Leukemia.
REFERENCE   3  (bases 1 to 5126)
  AUTHORS   Chen,H.Y., Cheng,H.L., Lee,Y.H., Yuan,T.M., Chen,S.W., Lin,Y.Y. and
            Chueh,P.J.
  TITLE     Tumor-associated NADH oxidase (tNOX)-NAD+-sirtuin 1 axis
            contributes to oxaliplatin-induced apoptosis of gastric cancer
            cells
  JOURNAL   Oncotarget 8 (9), 15338-15348 (2017)
   PUBMED   28122359
  REMARK    GeneRIF: The tumor-associated NADH oxidase (tNOX)-mediated
            modulation of the NAD+ concentration and SIRT1 are involved in
            oxaliplatin-induced apoptosis.
REFERENCE   4  (bases 1 to 5126)
  AUTHORS   Chen,H.Y., Lee,Y.H., Chen,H.Y., Yeh,C.A., Chueh,P.J. and Lin,Y.M.
  TITLE     Capsaicin Inhibited Aggressive Phenotypes through Downregulation of
            Tumor-Associated NADH Oxidase (tNOX) by POU Domain Transcription
            Factor POU3F2
  JOURNAL   Molecules 21 (6), 733 (2016)
   PUBMED   27271588
  REMARK    GeneRIF: These findings not only shed light on the molecular
            mechanism of the anticancer properties of capsaicin, but also the
            transcription regulation of tNOX expression that may potentially
            explain how POU3F2 is associated with tumorigenesis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5126)
  AUTHORS   Meadows,C., Morre,D.J., Morre,D.M., Draelos,Z.D. and Kern,D.
  TITLE     Age-related NADH oxidase (arNOX)-catalyzed oxidative damage to skin
            proteins
  JOURNAL   Arch Dermatol Res 306 (7), 645-652 (2014)
   PUBMED   24906676
  REMARK    GeneRIF: The findings support a role for arNOX as a major source of
            oxidative damage leading to cross-linking of skin proteins.
REFERENCE   6  (bases 1 to 5126)
  AUTHORS   Kishi,T., Morre,D.M. and Morre,D.J.
  TITLE     The plasma membrane NADH oxidase of HeLa cells has hydroquinone
            oxidase activity
  JOURNAL   Biochim Biophys Acta 1412 (1), 66-77 (1999)
   PUBMED   10354495
REFERENCE   7  (bases 1 to 5126)
  AUTHORS   Morre,D.J., Chueh,P.J., Lawler,J. and Morre,D.M.
  TITLE     The sulfonylurea-inhibited NADH oxidase activity of HeLa cell
            plasma membranes has properties of a protein disulfide-thiol
            oxidoreductase with protein disulfide-thiol interchange activity
  JOURNAL   J Bioenerg Biomembr 30 (5), 477-487 (1998)
   PUBMED   9932650
REFERENCE   8  (bases 1 to 5126)
  AUTHORS   Dai,S., Morre,D.J., Geilen,C.C., Almond-Roesler,B., Orfanos,C.E.
            and Morre,D.M.
  TITLE     Inhibition of plasma membrane NADH oxidase activity and growth of
            HeLa cells by natural and synthetic retinoids
  JOURNAL   Mol Cell Biochem 166 (1-2), 101-109 (1997)
   PUBMED   9046026
REFERENCE   9  (bases 1 to 5126)
  AUTHORS   Chang,K. and Pastan,I.
  TITLE     Molecular cloning and expression of a cDNA encoding a protein
            detected by the K1 antibody from an ovarian carcinoma (OVCAR-3)
            cell line
  JOURNAL   Int J Cancer 57 (1), 90-97 (1994)
   PUBMED   8150545
REFERENCE   10 (bases 1 to 5126)
  AUTHORS   Tarasoutchi,F., Grinberg,M., de Figueiredo Neto,J.A., de
            Assis,R.V., Cardoso,L.F., Parga Filho,J.R. and Bellotti,G.
  TITLE     [Mitral valve aneurysm associated with mitral insufficiency in
            absence of aortic insufficiency]
  JOURNAL   Arq Bras Cardiol 56 (3), 231-234 (1991)
   PUBMED   1888291
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK289837.1, BC140874.1,
            AI540639.1 and AL591908.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 14, 2020 this sequence version replaced NM_006375.3.
            
            Summary: This gene is a tumor-specific member of the ECTO-NOX
            family of genes that encode cell surface NADH oxidases. The encoded
            protein has two enzymatic activities: catalysis of hydroquinone or
            NADH oxidation, and protein disulfide interchange. The protein also
            displays prion-like properties. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (1) differs in the 5' UTR and the
            5' coding region and initiates translation at a downstream start
            codon, compared to variant 2. Variants 1 and 3 encode the same
            isoform (a) which is shorter at the N-terminus, compared to isoform
            b.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK289837.1, SRR14038193.1920050.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000394363.6/ ENSP00000377890.1
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1713              AK289837.1         1-1713
            1714-3994           BC140874.1         1592-3872
            3995-4072           AI540639.1         3-80                c
            4073-5126           AL591908.5         61330-62383         c
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq26.1"
     gene            1..5126
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /note="ecto-NOX disulfide-thiol exchanger 2"
                     /db_xref="GeneID:10495"
                     /db_xref="HGNC:HGNC:2259"
                     /db_xref="MIM:300282"
     exon            1..161
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            162..209
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            210..353
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            354..488
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     CDS             392..2137
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /note="isoform a is encoded by transcript variant 1;
                     cytosolic ovarian carcinoma antigen 1; APK1 antigen;
                     tumor-associated hydroquinone oxidase; tumor-associated
                     NADH oxidase; ecto-NADPH oxidase disulfide-thiol exchanger
                     2"
                     /codon_start=1
                     /product="ecto-NOX disulfide-thiol exchanger 2 isoform a"
                     /protein_id="NP_006366.2"
                     /db_xref="CCDS:CCDS14627.1"
                     /db_xref="GeneID:10495"
                     /db_xref="HGNC:HGNC:2259"
                     /db_xref="MIM:300282"
                     /translation="MTLPMSDPTAWATAMNNLGMAPLGIAGQPILPDFDPALGMMTGI
                     PPITPMMPGLGIVPPPIPPDMPVVKEIIHCKSCTLFPPNPNLPPPATRERPPGCKTVF
                     VGGLPENGTEQIIVEVFEQCGEIIAIRKSKKNFCHIRFAEEYMVDKALYLSGYRIRLG
                     SSTDKKDTGRLHVDFAQARDDLYEWECKQRMLAREERHRRRMEEERLRPPSPPPVVHY
                     SDHECSIVAEKLKDDSKFSEAVQTLLTWIERGEVNRRSANNFYSMIQSANSHVRRLVN
                     EKAAHEKDMEEAKEKFKQALSGILIQFEQIVAVYHSASKQKAWDHFTKAQRKNISVWC
                     KQAEEIRNIHNDELMGIRREEEMEMSDDEIEEMTETKETEESALVSQAEALKEENDSL
                     RWQLDAYRNEVELLKQEQGKVHREDDPNKEQQLKLLQQALQGMQQHLLKVQEEYKKKE
                     AELEKLKDDKLQVEKMLENLKEKESCASRLCASNQDSEYPLEKTMNSSPIKSEREALL
                     VGIISTFLHVHPFGASIEYICSYLHRLDNKICTSDVECLMGRLQHTFKQEMTGVGASL
                     EKRWKFCGFEGLKLT"
     exon            489..644
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            645..851
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            852..1085
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1298
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1405
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1520
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1702
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1810
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..1919
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..2005
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            2006..5126
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3526..3531
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /note="hexamer: GATAAA"
     polyA_site      3550
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /note="major polyA site"
     regulatory      5019..5024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /note="hexamer: ATTAAA"
     polyA_site      5041
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
     regulatory      5101..5106
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
                     /note="hexamer: AATAAA"
     polyA_site      5126
                     /gene="ENOX2"
                     /gene_synonym="APK1; COVA1; tNOX"
ORIGIN      
        1 agagtgccgg gtctaggcct ggaagcgcga ggcgggcatt tcggaagaga gtggcgcggc
       61 gtggggcgcc gcgagggcac tacgagccca ggaactgccg cgccggcccg attttccgcg
      121 gctgtataag gactagcgct tcggtagccg gggagctgga gggaacctga tttgagatca
      181 attcttggag aacacaaaga agtgtgatgg attgaaccag ctgccctttg accctgctaa
      241 caacaacgag cccctgcagt ttggtagtgc cagtggccct ctggtcacag aaggcctcat
      301 tgagaatgga ggggaatcaa gcaagaaaag aaagagaaca aatactcctt cagccgataa
      361 cagtagaact ctgaacgtgg attccactgc aatgacacta cctatgtctg atccaactgc
      421 atgggccaca gcaatgaata atcttggaat ggcaccgctg ggaattgccg gacaaccaat
      481 tttacctgac tttgatcctg ctcttggaat gatgactgga attccaccaa taactccaat
      541 gatgcctggt ttgggaatag tacctccacc aattcctcca gatatgccag tagtaaaaga
      601 gatcatacac tgtaaaagct gcacgctctt ccctccaaat ccaaatctcc cacctcctgc
      661 aacccgagaa agaccaccag gatgcaaaac agtatttgtg ggtggtctgc ctgaaaatgg
      721 gacagagcaa atcattgtgg aagttttcga gcagtgtgga gagatcattg ccattcgcaa
      781 gagcaagaag aacttctgcc acattcgctt tgctgaggag tacatggtgg acaaagccct
      841 gtatctgtct ggttaccgca ttcgcctggg ctctagtact gacaagaagg acacaggcag
      901 actccacgtt gatttcgcac aggctcgaga tgacctgtat gagtgggagt gtaaacagcg
      961 tatgctagcc agagaggagc gccatcgtag aagaatggaa gaagaaagat tgcgtccacc
     1021 atctccaccc ccagtggtcc actattcaga tcatgaatgc agcattgttg ctgaaaaatt
     1081 aaaagatgat tccaaattct cagaagctgt acagaccttg cttacctgga tagagcgagg
     1141 agaggtcaac cgtcgtagcg ccaataactt ctactccatg atccagtcgg ccaacagcca
     1201 tgtccgccgc ctggtgaacg agaaagctgc ccatgagaaa gatatggaag aagcaaagga
     1261 gaagttcaag caggcccttt ctggaattct cattcaattt gagcagatag tggctgtgta
     1321 ccattccgcc tccaagcaga aggcatggga ccacttcaca aaagcccagc ggaagaacat
     1381 cagcgtgtgg tgcaaacaag ctgaggaaat tcgcaacatt cataatgatg aattaatggg
     1441 aatcaggcga gaagaagaaa tggaaatgtc tgatgatgaa atagaagaaa tgacagaaac
     1501 aaaagaaact gaggaatcag ccttagtatc acaggcagaa gctctgaagg aagaaaatga
     1561 cagcctccgt tggcagctcg atgcctaccg gaatgaagta gaactgctca agcaagaaca
     1621 aggcaaagtc cacagagaag atgaccctaa caaagaacag cagctgaaac tcctgcaaca
     1681 agccctgcaa ggaatgcaac agcatctact caaagtccaa gaggaataca aaaagaaaga
     1741 agctgaactt gaaaaactca aagatgacaa gttacaggtg gaaaaaatgt tggaaaatct
     1801 taaagaaaag gaaagctgtg cttctaggct gtgtgcctca aaccaggata gcgaataccc
     1861 tcttgagaag accatgaaca gcagtcctat caaatctgaa cgtgaagcac tgctagtggg
     1921 gattatctcc acattccttc atgttcaccc atttggagca agcattgaat acatctgttc
     1981 ctacttgcac cgtcttgata ataagatctg caccagcgat gtggagtgtc tcatgggtag
     2041 actccagcat accttcaagc aggaaatgac tggagttgga gccagcctgg aaaagagatg
     2101 gaaattctgt ggcttcgagg gcttgaagct gacctaaatc tctttgccta acaacttggg
     2161 atcctgaaga taaatatgtg ttggacaagc atagaaagtg atttatattt ttaatggttt
     2221 tcaagtggaa gttcctttga atttgtcagt tcattcctgg aaaatctttt gagttaaaat
     2281 aaggatccta ggacagcacc tcgaactaca ggccctaaag agaaattgcc tcaaaccaca
     2341 agtgctgtaa cttcctcccc tttctgtcaa ttggttgtct ttaaatattg caaaagtcct
     2401 gatgctaaac agtatttgga gtgttttcag tgtctgtact actgttgtag accttggtat
     2461 ttttttaaac actgttaact gaaatgtttt gatgatttgt atgtgatttg tgtttctaaa
     2521 cttctcttta cattaatgtt gttactggtg aaaggcatga gagcagcact aagtcctctg
     2581 tgtaactgcc attgtctttc caatccccag tagaccagta aataaataac acatcagtgt
     2641 cttctagaag gtgcctgacc aggttcacct tttaaacgac aaagcatggt ttgtggcttt
     2701 ttgcaaaatt actatgaacc aaaagttgac aaatgttcca aagttatttt ctctaacata
     2761 tcacattaaa gatctgtttc agaattgtaa aaagtacatc tagatgtgtt tacagaaagc
     2821 aagtatccag tatgactggc atgtgttcat gctattcaga atcacttgta aatagtctgc
     2881 ttttaaagga gggcatgttc agttttctgt gaattaaaat atgctcatgt gtgggcacac
     2941 acgcacaaac acacacacgc acgcacacag tggcagaagg gatttatatt aatattcttt
     3001 cccctctggc cttcttacag tctgttggtc cctttgcttc tgttgtcagt gtgttgaatt
     3061 gcaaaccgag tactgctgta aatactatgt ttacttcatg ctgaatgttt gcaaagactt
     3121 gatataagta ttaatagtaa tgaatcaatg aataaataat gagctagggt ttgtgaggct
     3181 ttctacaaat aggtcagctc cacctggagt gcgaattgcc agagacacct tggtagtgcc
     3241 catcggcaaa tcgcaatggc agcatgtgag tggaccattc agaaacttct gcttggtgga
     3301 aagtaaacag agaggatgga ggtttggggc gaatgtcctg aggcagagat ggtctttatt
     3361 gtgtgtggtg gtggttgtgg tatttataat aatgcaagca taccctccct tgagtctcaa
     3421 ttgaagataa aagaatgtac tgagcaagca aagccaatgg agagtatttc acaaaaatac
     3481 tttgtaaatg agatgccagt agtgttcaaa gttgtatttt taaaagataa atattccttt
     3541 ttatacctca gttttgtgtc ctgtttttaa tgacttacgc tctaagtaat ccattagtag
     3601 ttatctcagt tccttccttt gggttactag aatgttggaa aaagatgcca agtctgtctt
     3661 gacaactgga aacagggttc cacagcagcc cattcgtgct gaaaactggc ttccccctga
     3721 agcaccctgc tgtggcacca gcaggaagct caggttaatt ttacactagc ttgctcactg
     3781 atgcatctct catcaatgct acggaaggct ttgattcatc agtctcgggc tcttggaata
     3841 cctaatttta ataatatcta tgaaatcaag ggaaactttc catttacagt tatttcttgt
     3901 ttaaataaac taaattaatt tttaggggag agcagtagga aaaagagcta atgcatgcgg
     3961 ggtttaatac ctaggtgatg ggttgaggtg cagcaaaacc accatggcac acgttcacct
     4021 atgtaacaaa cctgcacatc ctgcacatgt accccggaac ttacttaaaa aataaaagaa
     4081 aaaaaatcaa actccttacc atgatattaa tacattaaaa aaaattttta aagcaacagc
     4141 tgccacacct aaatacctta ctctccttca cttaatcaac ttacattgca gattgtctgt
     4201 ttctgttact cctgccagtt tagtataggt atactcctcc cagtttgctt gaatatacta
     4261 gtttttatgg ttaattttgt gtggcaactt ggctaggcta tagtgcccag ttgcttggtc
     4321 aaacaccagt ctagatattg ctgtgaaggt atattttaaa tgtgattaac atttaagcca
     4381 gtagactttg agtaaaacag attacccatt ataatgtggg tggacctcat ctgatcagct
     4441 gaaggcctta agagaaaaga ctgaagtccc ctgaggaggg aattctgctt cgagactgcc
     4501 tttggactca aaactgcaac atccaactcc tgccagaact tccagcctgc cggcctgccc
     4561 tacaaatctc taacttgcca gcccccataa ttgcaagagc caattcctta aaataaatag
     4621 atctctctca tatatatcta tatatagata agcatagata tatacatatg aatatacatt
     4681 atattacaca atcaaattgt ggcacatacc atctctcaca aaccttttca tccatcatag
     4741 aaatctatta atgaagctgc tttttcctac aaagtctcag tgctatttat ggttctgttg
     4801 ttcctccaga attttgaaat attgctgttt tgttatgttt atgagataca gtagttagcg
     4861 tttcttcaat ataataatga atggaaggtt tcttttgttt tacaaatgat ttttttatct
     4921 ccttccctag tcctgtgaca tctctaattc tgactttatt attaccaagt tgcttgttaa
     4981 atgtcttcaa tgcaaatgaa ggttattttt catagaaaat taaagacaca tctgcattca
     5041 agttatatag agtttttttt taattttggc cccaaaatgt atgcttttca tataaggcag
     5101 aataaactgt attatttgaa aagata
//
