LOCUS       NM_001354066            5320 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens enolase superfamily member 1 (ENOSF1), transcript
            variant 11, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001354066 XM_017025833
VERSION     NM_001354066.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Arjmandi,K., Ameli,F., Salahshourifar,I., Esfandbod,M. and Irani,S.
  TITLE     Evaluation of TS and ENOSF1 Variants as a Biomarker in Response to
            Neoadjuvant Chemotherapy based on 5FU in Gastric Cancer Patients
  JOURNAL   Asian Pac J Cancer Prev 23 (9), 2983-2989 (2022)
   PUBMED   36172660
  REMARK    GeneRIF: Evaluation of TS and ENOSF1 Variants as a Biomarker in
            Response to Neoadjuvant Chemotherapy based on 5FU in Gastric Cancer
            Patients.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 5320)
  AUTHORS   Hamzic,S., Kummer,D., Froehlich,T.K., Joerger,M., Aebi,S.,
            Palles,C., Thomlinson,I., Meulendijks,D., Schellens,J.H.M.,
            Garcia-Gonzalez,X., Lopez-Fernandez,L.A., Amstutz,U. and
            Largiader,C.R.
  TITLE     Evaluating the role of ENOSF1 and TYMS variants as predictors in
            fluoropyrimidine-related toxicities: An IPD meta-analysis
  JOURNAL   Pharmacol Res 152, 104594 (2020)
   PUBMED   31838077
  REMARK    GeneRIF: Evaluating the role of ENOSF1 and TYMS variants as
            predictors in fluoropyrimidine-related toxicities: An IPD
            meta-analysis.
REFERENCE   4  (bases 1 to 5320)
  AUTHORS   Giusti,B., Sestini,I., Saracini,C., Sticchi,E., Bolli,P., Magi,A.,
            Gori,A.M., Marcucci,R., Gensini,G.F. and Abbate,R.
  TITLE     High-throughput multiplex single-nucleotide polymorphism (SNP)
            analysis in genes involved in methionine metabolism
  JOURNAL   Biochem Genet 46 (7-8), 406-423 (2008)
   PUBMED   18427977
  REMARK    GeneRIF: Observational study of genetic testing. (HuGE Navigator)
REFERENCE   5  (bases 1 to 5320)
  AUTHORS   Liang,P., Nair,J.R., Song,L., McGuire,J.J. and Dolnick,B.J.
  TITLE     Comparative genomic analysis reveals a novel mitochondrial isoform
            of human rTS protein and unusual phylogenetic distribution of the
            rTS gene
  JOURNAL   BMC Genomics 6, 125 (2005)
   PUBMED   16162288
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5320)
  AUTHORS   Dolnick,B.J., Angelino,N.J., Dolnick,R. and Sufrin,J.R.
  TITLE     A novel function for the rTS gene
  JOURNAL   Cancer Biol Ther 2 (4), 364-369 (2003)
   PUBMED   14508106
REFERENCE   7  (bases 1 to 5320)
  AUTHORS   Chu,J. and Dolnick,B.J.
  TITLE     Natural antisense (rTSalpha) RNA induces site-specific cleavage of
            thymidylate synthase mRNA
  JOURNAL   Biochim Biophys Acta 1587 (2-3), 183-193 (2002)
   PUBMED   12084460
  REMARK    Review article
REFERENCE   8  (bases 1 to 5320)
  AUTHORS   Dolnick,B.J., Lu,K., Yin,M.B. and Rustum,Y.M.
  TITLE     Recent advances in the study of rTS proteins. rTS expression during
            growth and in response to thymidylate synthase inhibitors in human
            tumor cells
  JOURNAL   Adv Enzyme Regul 37, 95-109 (1997)
   PUBMED   9381988
REFERENCE   9  (bases 1 to 5320)
  AUTHORS   Dolnick,B.J. and Black,A.R.
  TITLE     Alternate splicing of the rTS gene product and its overexpression
            in a 5-fluorouracil-resistant cell line
  JOURNAL   Cancer Res 56 (14), 3207-3210 (1996)
   PUBMED   8764108
REFERENCE   10 (bases 1 to 5320)
  AUTHORS   Dolnick,B.J.
  TITLE     Cloning and characterization of a naturally occurring antisense RNA
            to human thymidylate synthase mRNA
  JOURNAL   Nucleic Acids Res 21 (8), 1747-1752 (1993)
   PUBMED   8493092
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP001178.4.
            
            On May 31, 2019 this sequence version replaced NM_001354066.1.
            
            Summary: This gene can encode a mitochondrial enzyme that is
            thought to convert L-fuconate to 2-keto-3-deoxy-L-fuconate. This
            locus was originally identified as the source of antisense RNAs of
            the adjacent thymidylate synthase gene. Splice variants at this
            locus may contain an alternate 3' exon that is complementary to the
            3'UTR and terminal intron of the thymidylate synthase (TS) RNA and
            may downregulate TS expression. [provided by RefSeq, Aug 2017].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189652.263038.1,
                                           ERR279854.2326.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 16162288
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-127               AP001178.4         140190-140316       c
            128-236             AP001178.4         134156-134264       c
            237-352             AP001178.4         124926-125041       c
            353-439             AP001178.4         121934-122020       c
            440-466             AP001178.4         121568-121594       c
            467-539             AP001178.4         118890-118962       c
            540-578             AP001178.4         118754-118792       c
            579-661             AP001178.4         118235-118317       c
            662-696             AP001178.4         116260-116294       c
            697-784             AP001178.4         113607-113694       c
            785-919             AP001178.4         110932-111066       c
            920-1049            AP001178.4         105429-105558       c
            1050-1149           AP001178.4         105031-105130       c
            1150-1231           AP001178.4         103007-103088       c
            1232-5320           AP001178.4         98004-102092        c
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.32"
     gene            1..5320
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /note="enolase superfamily member 1"
                     /db_xref="GeneID:55556"
                     /db_xref="HGNC:HGNC:30365"
                     /db_xref="MIM:607427"
     exon            1..127
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..1333
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /EC_number="4.2.1.68"
                     /note="isoform 5 is encoded by transcript variant 11;
                     antisense RNA to thymidylate synthase; mitochondrial
                     enolase superfamily member 1; L-fuconate dehydratase"
                     /codon_start=1
                     /product="mitochondrial enolase superfamily member 1
                     isoform 5"
                     /protein_id="NP_001340995.1"
                     /db_xref="GeneID:55556"
                     /db_xref="HGNC:HGNC:30365"
                     /db_xref="MIM:607427"
                     /translation="MVRGRISRLSVRDVRFPTSLGGHGADAMHTDPDYSAAYVVIETD
                     AEDGIKGCGITFTLGKGTEVVVCAVNALAHHVLNKDLKDIVGDFRGFYRQLTSDGQLR
                     WIGPEKGVVHLATAAVLNAVWDLWAKQEGKPVWKLLVDMDPRMLVSCIDFRYITDVLT
                     EEDALEILQKGQIGKKEREKQMLAQGYPAYTTSCAWLGYSDDTLKQLCAQALKDGWTR
                     FKVKVGADLQDDMRRCQIIRDMIGPEKTLMMDANQRWDVPEAVEWMSKLAKFKPLWIE
                     EPTSPDDILGHATISKCHNRVIFKQLLQAKALQFLQIDSCRLGSVNENLSVLLMAKKF
                     EIPVCPHAGGVGLCELVQHLIIFDYISVSASLENRVCEYVDHLHEHFKYPVMIQRASY
                     MPPKDPGYSTEMKEESVKKHQYPDGEVWKKLLPAQEN"
     exon            128..236
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     exon            237..352
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     exon            353..439
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     exon            440..466
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     exon            467..539
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     exon            540..578
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     exon            579..661
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     exon            662..696
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     exon            697..784
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     exon            785..919
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1049
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1149
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1231
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..5320
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1615..1620
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /note="hexamer: ATTAAA"
     polyA_site      1637
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /note="major polyA site"
     regulatory      1704..1709
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /note="hexamer: AATAAA"
     polyA_site      1724
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
     regulatory      3060..3065
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /note="hexamer: ATTAAA"
     polyA_site      3089
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
     regulatory      4260..4265
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /note="hexamer: TATAAA"
     polyA_site      4284
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
     regulatory      5242..5247
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /note="hexamer: AATAAT"
     polyA_site      5265
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
     regulatory      5292..5297
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
                     /note="hexamer: ATTAAA"
     polyA_site      5320
                     /gene="ENOSF1"
                     /gene_synonym="FUCD; RTS; TYMSAS"
ORIGIN      
        1 agtcctgacc gcacgggggc cgcggccacg gggcgcaggg gccatggtgc gcggcaggat
       61 ctcccggctc tcggtccggg acgtgcgctt ccccacgtcg cttgggggcc acggcgcgga
      121 cgccatgcac acggaccctg actactcggc tgcctatgtc gtcatagaaa ctgatgcaga
      181 agatggaatc aaggggtgtg gaattacctt cactctggga aaaggcactg aagttgttgt
      241 ctgtgctgtg aatgccctcg cccaccatgt gctcaacaag gacctcaagg acattgttgg
      301 tgacttcaga ggcttctata ggcagctcac aagtgatggg cagctcagat ggattggtcc
      361 agaaaagggc gtggtgcacc tggcgacagc ggccgtccta aacgcggtgt gggacttgtg
      421 ggccaagcag gagggaaagc ctgtctggaa gttacttgtg gacatggatc ccaggatgct
      481 ggtatcctgc atagatttca ggtacatcac tgatgtcctg actgaggagg atgccctaga
      541 aatactgcag aaaggtcaaa ttggtaaaaa agaaagagag aagcaaatgc tggcacaagg
      601 ataccctgct tacacgacat cgtgcgcctg gctggggtac tcagatgaca cgttgaagca
      661 gctctgtgcc caggcgctga aggatggctg gaccaggttt aaagtaaagg tgggtgctga
      721 tctccaggat gacatgcgaa gatgccaaat catccgagac atgattggac cggaaaagac
      781 tttgatgatg gatgccaacc agcgctggga tgtgcctgag gcggtggagt ggatgtccaa
      841 gctggccaag ttcaagccat tgtggattga ggagccaacc tcccctgatg acattctggg
      901 gcacgccacc atttccaagt gccacaatag agtgatattt aagcaactcc tacaggcgaa
      961 ggccctgcag ttcctccaga ttgacagttg cagactgggc agtgtcaatg agaacctctc
     1021 agtattgctg atggccaaaa agtttgaaat tcctgtttgc ccccatgctg gtggagttgg
     1081 cctctgtgaa ctggtgcagc acctgattat atttgactac atatcagttt ctgcaagcct
     1141 tgaaaatagg gtgtgtgagt atgttgacca cctgcatgag catttcaagt atcccgtgat
     1201 gatccagcgg gcttcctaca tgcctcccaa ggatcccggc tactcaacag aaatgaagga
     1261 ggaatctgta aagaaacacc agtatccaga tggtgaagtt tggaagaaac tccttcctgc
     1321 tcaagaaaat taagtgctca gccccaacaa cttttttctt tctgaagtga aagggcttaa
     1381 aatttcttgg aaatagtttt acaaaaatgg atttaaaaaa tcctaccgat caagatgagt
     1441 tcagctagaa gtcataccac cctcaggaat cagctaagta attattactt gattctttta
     1501 gcaaatcaat gcacgttatc ctacttaatc cttaaataag tttagattta actaacccaa
     1561 agtccaggag gatgttctta caaaaatagc tatatcaagg gctggcacct agacattaaa
     1621 ctgtaatttg aaaataagca acatgttgca taacttgttg gaataattcc ttgttctgtt
     1681 taacacttgt cataaattag cagaataaaa atagtcgtgc aacaccgggg gtatctggta
     1741 tgcaacgaag ggaaaaatat ttcactgatt aaccccgaag tggttttgca tcttttcctt
     1801 gcttaatcta agcatattat tagagaagtc acaccatgct gaagctaatg agggcaaaat
     1861 ggtagtccat agattatttt aaaataaccc tttaaggtta taaaagttta aaaaaaaaaa
     1921 aaaaaaactc tatcctaaat ggtcattata ttttgaggat aagatgcagt taaaatgaga
     1981 aaaatagggc aaaatatatt cactattatt tctaaaatat actcttttaa gtagcatcca
     2041 aaccagaata cagcacatgt ttacttaagg agagttcttt aatctatttt aggaaggaac
     2101 tgagcagata agtggcagta cagaatgaac aaagcgtgga cgaatgcaga acacttcttt
     2161 attatagcaa catataaaac aactataact ttaaagttca taaccacact ctacatcatg
     2221 atcgatggtg ttactcagct ccctcagatt tgagggaata gcttgtgaaa ttcttaaaat
     2281 attctaaaaa tattccaaaa atagcttgtg aaattcacca accttcttta taagtacgtg
     2341 ggattgaaat gcacatacat gtttttgcta agagcacata catttcattc tcctcacttt
     2401 gttcataacc tcagcattgt cagataccct cagtgagtta actcaaagcc ttttattatg
     2461 gaaagaactg gcacagttac atttgccagt ggcaacatcc ttaaaaatta ataactgata
     2521 ggtcacggac agatttttga cctagttcct ttttctttta gagcaaaaag aacttttacc
     2581 tcggcatcca gcccaacccc taaagactga caatatcctt cgagctcctt tgaaagcacc
     2641 ctaaacagcc atttccattt taatagttgg atgcggattg tacccttcaa tctgaaagtc
     2701 ttcagctttg aagtcatcaa ttttctcaac ttttcgaaga atcctgagct ttgggaaagg
     2761 tctgggttct cgctgaagct aaaaacaaaa taaggccatt attttgccat aattgtacga
     2821 cctgttgtaa ttgctcctca tgtccgtgaa acaagtacac aggatgtgat caacaaagtt
     2881 ctattttaca ggagtatgat cctgtcgata ccttgccgta ggttatgtaa catgattgga
     2941 gcgcaaccag ctgttctctt gcacagatcg agagtgaggg gtattttgtg acattacaca
     3001 gcatcaggag cctggtgcct catcaggtgt aagttcttat aaccactctt ggcaaattta
     3061 ttaaagacag gaacacagtc aatctgtaac tcatagtagc tctacgttta cttgaattcc
     3121 acaatcccta acccatctgt ccctggcaga aagaaggaaa gatgacatgc atggacagtg
     3181 aacagaaagg gatgaaagcc aggattcctg ggatgaacag acagtggcaa ttaggatgtg
     3241 aagacaggtc acaacctatt actatgtcta aaaacgacca gagcagagag ccagagagaa
     3301 taagcctgaa gtcacctcca ctcaaaagca gccaaactcc ctcaaaggag taacttttaa
     3361 aacctggatc taacctggaa ggggctaaaa agtgtctggt tctgagtttt tttccttaag
     3421 gctcatgaag cagatgaact tacattttta ttgccatttc atatcaattg ttggctgcta
     3481 taacttaggg atttcaacag acttttgaag tttggaccta aatattgtac ttaatgtaaa
     3541 attaacaaaa aatatttatg gccagggtgg tggcttatgc ctgtaattcc agaattttcg
     3601 gaggctgagg caggtggatc acttgaagtc aggagtttga gactagcctg gccaacatga
     3661 tgaaacccca tctctactaa taatacaaaa attagctggg tgtggtggca tgtgcctgta
     3721 atcccagcta cctgggaggc tgaggcagaa gaattgcttg aacccgggag gtggaggttg
     3781 cagtgagctg agatcgcacc acggcacact ccagcctggc cgacagagaa agactccatc
     3841 tcaaaaaaaa aagaaaagga aaaacatttg cacttcaatt ctccttcaag ttaaaatgag
     3901 ttaaaatgcc cccttttgga caatcccctg gcttgaatgt ggctcttccc tctctggtac
     3961 tggtgcttag tacctcacag cacctgacat gttaagtgcc catggttgct gaggcagatg
     4021 cctgccttgt cctgcccacc tgcccaccac ttctccctaa actgaagccc cacatttgga
     4081 gcagtcatct ttatcttgga cacagcattg agcagatgcc tgttccacag tcaacctttt
     4141 atcaagagaa ggtaccaaac ccaaaagtat aacatctaat tcttacctga attttcagtg
     4201 gctcgatgtg attcaggtaa atatgtgcat ctcccaaagt gtgtataaag tcacctggct
     4261 ataaacccgg gggagaaagc agaacagtat gttagtttca attctttaaa acatcattta
     4321 aaaacattag aatatgcaga caccgcaagg ctttttttaa aaaaataatt tagtgtagct
     4381 tttccatttt tttgtagcaa cagcatcttg ttatgttgcc caggctggta ttgaactcca
     4441 gacctcaagc aattgctcct gtctcagtct cccaaagtgc tgggattaca ggcatgagcc
     4501 accataccca acctcagcat agcttttgag aaaatccata gaagctgtat cacaaacaac
     4561 ctgtatagat ctgttagtgc gtataccaca gggccagaaa accttccaga agaggaaggt
     4621 ttcaaagtaa aagctggttc atttcttact tacacatatc aaatttaaaa gctaatcaga
     4681 gactaaactc tgcaatttgt tttcccatat taaagaactg aagagctcag tgtggtaggc
     4741 tggcaagtca cccttcccga gacagcccac cttcaggccc gtgatgtgcg caatcatgta
     4801 cgtgagcagg gcgtagctgg cgatgttgaa aggcacaccg aggcccatgt ctcccgatct
     4861 ctggtacagc tggcaggaca gctcactgtt caccacatag aactggcaga gggcatggca
     4921 tggaggcagc gccatcagag gaagatctga ggaaccagca gaggaagata aggagggatg
     4981 gtggtttgaa agaccacagc taaaggcaaa gtaaaacagg agagaaacag aagccaactc
     5041 atatggtgga gaccaggaga gagagccact gggctgcagt gatgtccata acagcctctg
     5101 cagcgatggc acggagctga gggagactat ccatcggtgc aaggtttctg caggtgtcca
     5161 tttacggctg aagcaatgct cttccatcag agctgaaggg atctgggcta cctcgtggca
     5221 ccagattaca aatacagcag gaataattct gtttgccaca ggaaactggt gcttctggta
     5281 caccctccta tattaaaagt ctctattaca tggccaggca
//
