LOCUS       NM_021204               2083 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens enolase-phosphatase 1 (ENOPH1), transcript variant 1,
            mRNA.
ACCESSION   NM_021204
VERSION     NM_021204.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Bu,Y., Hao,J., He,J., Li,X., Liu,Y. and Ma,L.
  TITLE     Tumor-promoting properties of enolase-phosphatase 1 in breast
            cancer via activating the NF-kappaB signaling pathway
  JOURNAL   Mol Biol Rep 50 (2), 993-1004 (2023)
   PUBMED   36378417
  REMARK    GeneRIF: Tumor-promoting properties of enolase-phosphatase 1 in
            breast cancer via activating the NF-kappaB signaling pathway.
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2083)
  AUTHORS   Wang,B., Xu,X., Liu,X., Wang,D., Zhuang,H., He,X., Han,T. and
            Hong,J.
  TITLE     Enolase-phosphatase 1 acts as an oncogenic driver in glioma
  JOURNAL   J Cell Physiol 236 (2), 1184-1194 (2021)
   PUBMED   32654229
  REMARK    GeneRIF: Enolase-phosphatase 1 acts as an oncogenic driver in
            glioma.
REFERENCE   4  (bases 1 to 2083)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2083)
  AUTHORS   Zhuang,H., Qiang,Z., Shao,X., Wang,H., Dang,Y., Wang,Z., Wu,F.,
            Wei,W. and Li,Y.
  TITLE     Integration of metabolomics and expression of enolase-phosphatase 1
            links to hepatocellular carcinoma progression
  JOURNAL   Theranostics 9 (12), 3639-3652 (2019)
   PUBMED   31281503
  REMARK    GeneRIF: Taken together, these findings illustrate that ENOPH1
            promotes hepatocellular carcinoma progression.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2083)
  AUTHORS   Su,L., Yang,K., Li,S., Liu,C., Han,J., Zhang,Y. and Xu,G.
  TITLE     Enolase-phosphatase 1 as a novel potential malignant glioma
            indicator promotes cell proliferation and migration
  JOURNAL   Oncol Rep 40 (4), 2233-2241 (2018)
   PUBMED   30066900
  REMARK    GeneRIF: High ENOPH1 expression is associated with malignant
            glioma.
REFERENCE   7  (bases 1 to 2083)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   8  (bases 1 to 2083)
  AUTHORS   Wang,H., Pang,H., Bartlam,M. and Rao,Z.
  TITLE     Crystal structure of human E1 enzyme and its complex with a
            substrate analog reveals the mechanism of its phosphatase/enolase
            activity
  JOURNAL   J Mol Biol 348 (4), 917-926 (2005)
   PUBMED   15843022
  REMARK    GeneRIF: the crystal structure of MASA and its complex with a
            substrate analog
REFERENCE   9  (bases 1 to 2083)
  AUTHORS   Hu,R.M., Han,Z.G., Song,H.D., Peng,Y.D., Huang,Q.H., Ren,S.X.,
            Gu,Y.J., Huang,C.H., Li,Y.B., Jiang,C.L., Fu,G., Zhang,Q.H.,
            Gu,B.W., Dai,M., Mao,Y.F., Gao,G.F., Rong,R., Ye,M., Zhou,J.,
            Xu,S.H., Gu,J., Shi,J.X., Jin,W.R., Zhang,C.K., Wu,T.M.,
            Huang,G.Y., Chen,Z., Chen,M.D. and Chen,J.L.
  TITLE     Gene expression profiling in the human
            hypothalamus-pituitary-adrenal axis and full-length cDNA cloning
  JOURNAL   Proc Natl Acad Sci U S A 97 (17), 9543-9548 (2000)
   PUBMED   10931946
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA067292.1, BC065815.1, AW470971.1 and AC067942.6.
            
            On Nov 22, 2018 this sequence version replaced NM_021204.4.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.271742.1,
                                           SRR1660803.204736.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000273920.8/ ENSP00000273920.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-52                DA067292.1         111-162
            53-1993             BC065815.1         1-1941
            1994-2081           AW470971.1         1-88                c
            2082-2083           AC067942.6         25700-25701
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q21.22"
     gene            1..2083
                     /gene="ENOPH1"
                     /gene_synonym="E1; MASA; MST145; mtnC"
                     /note="enolase-phosphatase 1"
                     /db_xref="GeneID:58478"
                     /db_xref="HGNC:HGNC:24599"
     exon            1..324
                     /gene="ENOPH1"
                     /gene_synonym="E1; MASA; MST145; mtnC"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..1026
                     /gene="ENOPH1"
                     /gene_synonym="E1; MASA; MST145; mtnC"
                     /EC_number="3.1.3.77"
                     /note="isoform 1 is encoded by transcript variant 1;
                     acireductone synthase;
                     2,3-diketo-5-methylthio-1-phosphopentane phosphatase;
                     enolase-phosphatase E1"
                     /codon_start=1
                     /product="enolase-phosphatase E1 isoform 1"
                     /protein_id="NP_067027.1"
                     /db_xref="CCDS:CCDS3594.1"
                     /db_xref="GeneID:58478"
                     /db_xref="HGNC:HGNC:24599"
                     /translation="MVVLSVPAEVTVILLDIEGTTTPIAFVKDILFPYIEENVKEYLQ
                     THWEEEECQQDVSLLRKQAEEDAHLDGAVPIPAASGNGVDDLQQMIQAVVDNVCWQMS
                     LDRKTTALKQLQGHMWRAAFTAGRMKAEFFADVVPAVRKWREAGMKVYIYSSGSVEAQ
                     KLLFGHSTEGDILELVDGHFDTKIGHKVESESYRKIADSIGCSTNNILFLTDVTREAS
                     AAEEADVHVAVVVRPGNAGLTDDEKTYYSLITSFSELYLPSST"
     exon            325..426
                     /gene="ENOPH1"
                     /gene_synonym="E1; MASA; MST145; mtnC"
                     /inference="alignment:Splign:2.1.0"
     exon            427..629
                     /gene="ENOPH1"
                     /gene_synonym="E1; MASA; MST145; mtnC"
                     /inference="alignment:Splign:2.1.0"
     exon            630..762
                     /gene="ENOPH1"
                     /gene_synonym="E1; MASA; MST145; mtnC"
                     /inference="alignment:Splign:2.1.0"
     exon            763..886
                     /gene="ENOPH1"
                     /gene_synonym="E1; MASA; MST145; mtnC"
                     /inference="alignment:Splign:2.1.0"
     exon            887..2083
                     /gene="ENOPH1"
                     /gene_synonym="E1; MASA; MST145; mtnC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1973..1978
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENOPH1"
                     /gene_synonym="E1; MASA; MST145; mtnC"
                     /note="hexamer: AATAAA"
     polyA_site      1993
                     /gene="ENOPH1"
                     /gene_synonym="E1; MASA; MST145; mtnC"
                     /note="major polyA site"
     regulatory      2057..2062
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENOPH1"
                     /gene_synonym="E1; MASA; MST145; mtnC"
                     /note="hexamer: AACAAG"
     polyA_site      2083
                     /gene="ENOPH1"
                     /gene_synonym="E1; MASA; MST145; mtnC"
ORIGIN      
        1 gtctcgggct cctgccccgt cctgctcacg agttcagggc tcctgggcgg ccgccttttc
       61 cagttccagg tgtgcagaag tgtcctctcc ccacgcgcgg cgggctgcac ttggtcgctg
      121 gctccgagat cgcgcggggc cgccggaagc ccaagacggt accgggggcc gcagccgcag
      181 ccggcgccgc cctccgccct ccccaacagc aggccgagtc ccgtagcatc cggtagggaa
      241 atggtcgtgc tttcggtccc cgccgaagtc accgtgatcc tgttagatat cgaaggtacc
      301 acaaccccga ttgctttcgt gaaggacatt ttatttcctt acatcgaaga aaatgttaaa
      361 gagtatctgc agacacattg ggaagaagag gagtgccagc aggatgtcag tcttttgagg
      421 aaacaggctg aagaggacgc ccacctggat ggggctgttc ctatccctgc agcatctggg
      481 aatggagtgg atgatctgca acagatgatc caggccgtgg tagataatgt gtgctggcag
      541 atgtccctgg atcgaaagac cactgcactc aaacagctgc agggccacat gtggagggcg
      601 gcattcacag ctgggcgcat gaaagcagag ttctttgcag atgtagttcc agcagtcagg
      661 aagtggagag aggccggaat gaaggtgtac atctattcct cagggagtgt ggaggcacag
      721 aaactgttat tcgggcattc tacggaggga gatattcttg agcttgttga tggtcacttt
      781 gataccaaga ttggacacaa agtagagagt gaaagttacc gaaagattgc agacagcatt
      841 gggtgctcaa ccaacaacat tttgtttctg acagatgtta ctcgagaggc cagtgctgct
      901 gaggaagcag atgtgcacgt agctgtggtg gtgagaccag gcaacgcagg attaacagat
      961 gatgagaaga cttactacag cctcatcaca tccttcagtg aactatacct gccttcctca
     1021 acctagagaa gggttgttaa ggcagaccgc cctgttcccc agagttgtcc ctgtagtgtc
     1081 taggtttatt ctaatggtaa aagtaactta cttaaaaaac atatgtacac atatgtatgc
     1141 aagtatgtat atatgtgtat gctcagatta acttccatag gtacataagt gaaagaagtc
     1201 tcagttcagt gaacacaaaa cttatttaaa gatgctttat atgtagaaat tgtttcaaat
     1261 catactctaa cccttagtga gggcaaagtg tagttggtag aagaaattgc taaataccta
     1321 tctaatgtgc tatgtttatc aaatcgtgta ctaaaatgga aagctagttt tgagaaatta
     1381 ttcagaagcc ttgttatttt aaaaatgaaa tatttcaaag actgaatatt ttcaaagaaa
     1441 atgaataatt cattgccctt gtgatttaga agattataac agctgtattt catatttgcc
     1501 tccttatata tatcaaagac caaggtattt ccttctgctt caaaagaaca aaattgggaa
     1561 agaaaactca cttgagtctt gatcaaacaa gtgtctttta cttaagaaga aacttggtaa
     1621 tcattgtggc acccacagca agcagttgcc ttaccagtga aaaaggtgca ctgaggtaac
     1681 atctaaaaca gagatgtggt tcttaatgtt taacagaaca gttctaatcc tgccacgtgt
     1741 tatcattata gattttatag ttgcctttct aactacttag cacagtttga gaatacgtta
     1801 attgctattt actatttaaa aagttttact gaaatcagtc cataacatta agatgagccc
     1861 taatatgtaa gattttcctc tggaatggat gtgagaaatg taaattttat aacagcagta
     1921 tttatcctgg tttaattcta atacgatgtc atgttaattt catgttgtga ttaataaaag
     1981 cattttttct tcactcagtt ttatataggt tcctgaactc tatccacatg ccgccatagc
     2041 aaaagagaaa ctgaaaaaca agaatattta gctcttatct taa
//
