LOCUS       NM_053013               1453 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens enolase 3 (ENO3), transcript variant 2, mRNA.
ACCESSION   NM_053013
VERSION     NM_053013.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Chen,J., Zhang,Z., Ni,J., Sun,J., Ju,F., Wang,Z., Wang,L. and
            Xue,M.
  TITLE     ENO3 promotes colorectal cancer progression by enhancing cell
            glycolysis
  JOURNAL   Med Oncol 39 (5), 80 (2022)
   PUBMED   35477821
  REMARK    GeneRIF: ENO3 promotes colorectal cancer progression by enhancing
            cell glycolysis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 1453)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 1453)
  AUTHORS   Park,C., Lee,Y., Je,S., Chang,S., Kim,N., Jeong,E. and Yoon,S.
  TITLE     Overexpression and Selective Anticancer Efficacy of ENO3 in STK11
            Mutant Lung Cancers
  JOURNAL   Mol Cells 42 (11), 804-809 (2019)
   PUBMED   31697874
  REMARK    GeneRIF: Enolase 3 (ENO3) overexpression under serine/threonine
            kinase 11 (STK11) loss-of-function mutations implies that ENO3
            might be a selective anticancer target in STK11-mutant cancer.
REFERENCE   5  (bases 1 to 1453)
  AUTHORS   Baleva,M., Gowher,A., Kamenski,P., Tarassov,I., Entelis,N. and
            Masquida,B.
  TITLE     A Moonlighting Human Protein Is Involved in Mitochondrial Import of
            tRNA
  JOURNAL   Int J Mol Sci 16 (5), 9354-9367 (2015)
   PUBMED   25918939
  REMARK    GeneRIF: tRK1 forms a complex with human enolases and interacts
            with tRK1 and human pre-lysyl-tRNA synthetase (preKARS2)
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1453)
  AUTHORS   Lanfranchi,G., Muraro,T., Caldara,F., Pacchioni,B., Pallavicini,A.,
            Pandolfo,D., Toppo,S., Trevisan,S., Scarso,S. and Valle,G.
  TITLE     Identification of 4370 expressed sequence tags from a
            3'-end-specific cDNA library of human skeletal muscle by DNA
            sequencing and filter hybridization
  JOURNAL   Genome Res 6 (1), 35-42 (1996)
   PUBMED   8681137
REFERENCE   7  (bases 1 to 1453)
  AUTHORS   Giallongo,A., Venturella,S., Oliva,D., Barbieri,G., Rubino,P. and
            Feo,S.
  TITLE     Structural features of the human gene for muscle-specific enolase.
            Differential splicing in the 5'-untranslated sequence generates two
            forms of mRNA
  JOURNAL   Eur J Biochem 214 (2), 367-374 (1993)
   PUBMED   8513787
  REMARK    Erratum:[Eur J Biochem 1993 Dec 15;218(3):1095]
REFERENCE   8  (bases 1 to 1453)
  AUTHORS   Peshavaria,M. and Day,I.N.
  TITLE     Molecular structure of the human muscle-specific enolase gene
            (ENO3)
  JOURNAL   Biochem J 275 (Pt 2) (Pt 2), 427-433 (1991)
   PUBMED   1840492
REFERENCE   9  (bases 1 to 1453)
  AUTHORS   Cali,L., Feo,S., Oliva,D. and Giallongo,A.
  TITLE     Nucleotide sequence of a cDNA encoding the human muscle-specific
            enolase (MSE)
  JOURNAL   Nucleic Acids Res 18 (7), 1893 (1990)
   PUBMED   2336366
REFERENCE   10 (bases 1 to 1453)
  AUTHORS   Peshavaria,M., Hinks,L.J. and Day,I.N.
  TITLE     Structure of human muscle (beta) enolase mRNA and protein deduced
            from a genomic clone
  JOURNAL   Nucleic Acids Res 17 (21), 8862 (1989)
   PUBMED   2587223
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA082822.1 and BC017249.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_053013.3.
            
            Summary: This gene encodes one of the three enolase isoenzymes
            found in mammals. This isoenzyme is found in skeletal muscle cells
            in the adult where it may play a role in muscle development and
            regeneration. A switch from alpha enolase to beta enolase occurs in
            muscle tissue during development in rodents. Mutations in this gene
            have be associated glycogen storage disease. Alternatively spliced
            transcript variants encoding different isoforms have been
            described.[provided by RefSeq, Jul 2010].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.889796.1,
                                           SRR3476690.147146.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000519602.6/ ENSP00000430055.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-546               DA082822.1         31-576
            547-1453            BC017249.2         534-1440
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.2"
     gene            1..1453
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /note="enolase 3"
                     /db_xref="GeneID:2027"
                     /db_xref="HGNC:HGNC:3354"
                     /db_xref="MIM:131370"
     exon            1..64
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /inference="alignment:Splign:2.1.0"
     exon            65..151
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /inference="alignment:Splign:2.1.0"
     CDS             67..1371
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /EC_number="4.2.1.11"
                     /note="isoform 1 is encoded by transcript variant 2;
                     skeletal muscle enolase; 2-phospho-D-glycerate hydrolyase;
                     enolase 3 (beta, muscle); muscle enriched enolase;
                     muscle-specific enolase"
                     /codon_start=1
                     /product="beta-enolase isoform 1"
                     /protein_id="NP_443739.3"
                     /db_xref="CCDS:CCDS11062.1"
                     /db_xref="GeneID:2027"
                     /db_xref="HGNC:HGNC:3354"
                     /db_xref="MIM:131370"
                     /translation="MAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIY
                     EALELRDGDKGRYLGKGVLKAVENINNTLGPALLQKKLSVVDQEKVDKFMIELDGTEN
                     KSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAG
                     NKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNIL
                     ENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKL
                     GELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEK
                     KACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQ
                     IKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPKAK"
     exon            152..247
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /inference="alignment:Splign:2.1.0"
     exon            248..306
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /inference="alignment:Splign:2.1.0"
     exon            307..376
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /inference="alignment:Splign:2.1.0"
     exon            377..510
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /inference="alignment:Splign:2.1.0"
     exon            511..733
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /inference="alignment:Splign:2.1.0"
     exon            734..931
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1133
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1242
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1301
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1453
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1432..1437
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /note="hexamer: AATAAA"
     polyA_site      1453
                     /gene="ENO3"
                     /gene_synonym="GSD13; MSE"
                     /note="major polyA site"
ORIGIN      
        1 gacactgtcc cagctgccac ctagactcgg agctccatcc aaacctccag cgaagacatc
       61 ccagccatgg ccatgcagaa aatctttgcc cgggaaatct tggactccag gggcaacccc
      121 acggtggagg tggacctgca cacggccaag ggccgattcc gagcagctgt gcccagtggg
      181 gcttccacgg gtatctatga ggctctggaa ctaagagacg gagacaaagg ccgctacctg
      241 gggaaaggag tcctgaaggc tgtggagaac atcaacaata ctctgggccc tgctctgctg
      301 caaaagaaac taagcgttgt ggatcaagaa aaagttgaca aatttatgat tgagctagat
      361 gggaccgaga ataagtccaa gtttggggcc aatgccatcc tgggcgtgtc cttggccgtg
      421 tgtaaggcgg gagcagctga gaagggggtc cccctgtacc gccacatcgc agatctcgct
      481 gggaaccctg acctcatact cccagtgcca gccttcaatg tgatcaacgg gggctcccat
      541 gctggaaaca agctggccat gcaggagttc atgattctgc ctgtgggagc cagctccttc
      601 aaggaagcca tgcgcattgg cgccgaggtc taccaccacc tcaagggggt catcaaggcc
      661 aagtatggga aggatgccac caatgtgggt gatgaaggtg gcttcgcacc caacatcctg
      721 gagaacaatg aggccctgga gctgctgaag acggccatcc aggcggctgg ttacccagac
      781 aaggtggtga tcggcatgga tgtggcagca tctgagttct atcgcaatgg gaagtacgat
      841 cttgacttca agtcgcctga tgatcccgca cggcacatca ctggggagaa gctcggagag
      901 ctgtataaga gctttatcaa gaactatcct gtggtctcca tcgaagaccc ctttgaccag
      961 gatgactggg ccacttggac ctccttcctc tcgggggtga acatccagat tgtgggggat
     1021 gacttgacag tcaccaaccc caagaggatt gcccaggccg ttgagaagaa ggcctgcaac
     1081 tgtctgctgc tgaaggtcaa ccagatcggc tcggtgaccg aatcgatcca ggcgtgcaaa
     1141 ctggctcagt ctaatggctg gggggtgatg gtgagccacc gctctgggga gactgaggac
     1201 acattcattg ctgaccttgt ggtggggctc tgcacaggac agatcaagac tggcgccccc
     1261 tgccgctcgg agcgtctggc caaatacaac caactcatga ggatcgagga ggctcttggg
     1321 gacaaggcaa tctttgctgg acgcaagttc cgtaacccga aggccaagtg agaagctgga
     1381 ggctccagga ctccactgga cagacccagg tcttccagac ctgcttcctg aaataaacac
     1441 tggtgccaac caa
//
