LOCUS       NM_001975               2294 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens enolase 2 (ENO2), mRNA.
ACCESSION   NM_001975
VERSION     NM_001975.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Du,W., Li,Y., Wang,X., Xie,S., Ci,H., Zhou,J., Zhu,N., Chen,Z.,
            Zheng,Y. and Jia,H.
  TITLE     Circular RNA circESYT2 serves as a microRNA-665 sponge to promote
            the progression of hepatocellular carcinoma through ENO2
  JOURNAL   Cancer Sci 115 (8), 2659-2672 (2024)
   PUBMED   38710213
  REMARK    GeneRIF: Circular RNA circESYT2 serves as a microRNA-665 sponge to
            promote the progression of hepatocellular carcinoma through ENO2.
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Feng,J. and Fang,J.
  TITLE     HOXC6-mediated transcriptional activation of ENO2 promotes oral
            squamous cell carcinoma progression through the Warburg effect
  JOURNAL   J Biochem Mol Toxicol 38 (7), e23752 (2024)
   PUBMED   38923759
  REMARK    GeneRIF: HOXC6-mediated transcriptional activation of ENO2 promotes
            oral squamous cell carcinoma progression through the Warburg
            effect.
REFERENCE   3  (bases 1 to 2294)
  AUTHORS   Mochetti,M.M., Silva,E.G.P., Correa,A.A.F., Cabette,M.R.,
            Perissinotti,I.N., E Silva,L.O.J., Pessoa,A.S., de Oliveira,R.C.,
            da Silva,L.F.F., de Souza,H.P. and de Alencar,J.C.G.
  TITLE     Neuron-specific enolase at admission as a predictor for stroke
            volume, severity and outcome in ischemic stroke patients: a
            prognostic biomarker review
  JOURNAL   Sci Rep 14 (1), 2688 (2024)
   PUBMED   38302568
  REMARK    GeneRIF: Neuron-specific enolase at admission as a predictor for
            stroke volume, severity and outcome in ischemic stroke patients: a
            prognostic biomarker review.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2294)
  AUTHORS   Shao,R., Liu,C., Xue,R., Deng,X., Liu,L., Song,C., Xie,J., Tang,H.
            and Liu,W.
  TITLE     Tumor-derived Exosomal ENO2 Modulates Polarization of
            Tumor-associated Macrophages through Reprogramming Glycolysis to
            Promote Progression of Diffuse Large B-cell Lymphoma
  JOURNAL   Int J Biol Sci 20 (3), 848-863 (2024)
   PUBMED   38250157
  REMARK    GeneRIF: Tumor-derived Exosomal ENO2 Modulates Polarization of
            Tumor-associated Macrophages through Reprogramming Glycolysis to
            Promote Progression of Diffuse Large B-cell Lymphoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2294)
  AUTHORS   Gao,L., Xie,J., Zhang,H., Zheng,H., Zheng,W., Pang,C., Cai,Y. and
            Deng,B.
  TITLE     Neuron-specific enolase in hypertension patients with acute
            ischemic stroke and its value forecasting long-term functional
            outcomes
  JOURNAL   BMC Geriatr 23 (1), 294 (2023)
   PUBMED   37189072
  REMARK    GeneRIF: Neuron-specific enolase in hypertension patients with
            acute ischemic stroke and its value forecasting long-term
            functional outcomes.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2294)
  AUTHORS   Oliva,D., Cali,L., Feo,S. and Giallongo,A.
  TITLE     Complete structure of the human gene encoding neuron-specific
            enolase
  JOURNAL   Genomics 10 (1), 157-165 (1991)
   PUBMED   2045099
REFERENCE   7  (bases 1 to 2294)
  AUTHORS   Craig,S.P., Day,I.N., Thompson,R.J. and Craig,I.W.
  TITLE     Localisation of neurone-specific enolase (ENO2) to 12p13
  JOURNAL   Cytogenet Cell Genet 54 (1-2), 71-73 (1990)
   PUBMED   2249478
REFERENCE   8  (bases 1 to 2294)
  AUTHORS   Oliva,D., Barba,G., Barbieri,G., Giallongo,A. and Feo,S.
  TITLE     Cloning, expression and sequence homologies of cDNA for human gamma
            enolase
  JOURNAL   Gene 79 (2), 355-360 (1989)
   PUBMED   2792767
REFERENCE   9  (bases 1 to 2294)
  AUTHORS   McAleese,S.M., Dunbar,B., Fothergill,J.E., Hinks,L.J. and Day,I.N.
  TITLE     Complete amino acid sequence of the neurone-specific gamma isozyme
            of enolase (NSE) from human brain and comparison with the
            non-neuronal alpha form (NNE)
  JOURNAL   Eur J Biochem 178 (2), 413-417 (1988)
   PUBMED   3208766
REFERENCE   10 (bases 1 to 2294)
  AUTHORS   Van Obberghen,E., Kamholz,J., Bishop,J.G. 3rd, Zomzely-Neurath,C.,
            Lazzarini,R.A. and Lazzarini,R.A.
  TITLE     Human gamma enolase: isolation of a cDNA clone and expression in
            normal and tumor tissues of human origin
  JOURNAL   J Neurosci Res 19 (4), 450-456 (1988)
   PUBMED   3385803
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U47924.1.
            
            On Nov 26, 2018 this sequence version replaced NM_001975.2.
            
            Summary: This gene encodes one of the three enolase isoenzymes
            found in mammals. This isoenzyme, a homodimer, is found in mature
            neurons and cells of neuronal origin. A switch from alpha enolase
            to gamma enolase occurs in neural tissue during development in rats
            and primates. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.161086.1,
                                           SRR3476690.1104951.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000229277.6/ ENSP00000229277.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-80                U47924.1           126590-126669
            81-177              U47924.1           127831-127927
            178-273             U47924.1           128427-128522
            274-332             U47924.1           128681-128739
            333-402             U47924.1           129048-129117
            403-536             U47924.1           129591-129724
            537-759             U47924.1           129950-130172
            760-957             U47924.1           131576-131773
            958-1159            U47924.1           133590-133791
            1160-1268           U47924.1           134065-134173
            1269-1327           U47924.1           134353-134411
            1328-2294           U47924.1           134740-135706
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.31"
     gene            1..2294
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /note="enolase 2"
                     /db_xref="GeneID:2026"
                     /db_xref="HGNC:HGNC:3353"
                     /db_xref="MIM:131360"
     exon            1..80
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /inference="alignment:Splign:2.1.0"
     exon            81..177
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..1397
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /EC_number="4.2.1.11"
                     /note="neurone-specific enolase; neuron specific gamma
                     enolase; 2-phospho-D-glycerate hydrolyase; neural enolase;
                     neuron-specific enolase; 2-phospho-D-glycerate
                     hydro-lyase; epididymis secretory protein Li 279; enolase
                     2 (gamma, neuronal); neuronal enriched enolase"
                     /codon_start=1
                     /product="gamma-enolase"
                     /protein_id="NP_001966.1"
                     /db_xref="CCDS:CCDS8570.1"
                     /db_xref="GeneID:2026"
                     /db_xref="HGNC:HGNC:3353"
                     /db_xref="MIM:131360"
                     /translation="MSIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIY
                     EALELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTEN
                     KSKFGANAILGVSLAVCKAGAAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAG
                     NKLAMQEFMILPVGAESFRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNIL
                     ENSEALELVKEAIDKAGYTEKIVIGMDVAASEFYRDGKYDLDFKSPTDPSRYITGDQL
                     GALYQDFVRDYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNPKRIERAVEE
                     KACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETEDTFIADLVVGLCTGQ
                     IKTGAPCRSERLAKYNQLMRIEEELGDEARFAGHNFRNPSVL"
     exon            178..273
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /inference="alignment:Splign:2.1.0"
     exon            274..332
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /inference="alignment:Splign:2.1.0"
     exon            333..402
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /inference="alignment:Splign:2.1.0"
     exon            403..536
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /inference="alignment:Splign:2.1.0"
     exon            537..759
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /inference="alignment:Splign:2.1.0"
     exon            760..957
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1159
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1268
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1327
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..2294
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2274..2279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /note="hexamer: ATTAAA"
     polyA_site      2294
                     /gene="ENO2"
                     /gene_synonym="HEL-S-279; NSE"
                     /note="major polyA site"
ORIGIN      
        1 gccactcccg ctctctcagc gccgccgtcg ccaccgccac cgccaccgcc actaccaccg
       61 tctgagtctg cagtcccgag atcccagcca tcatgtccat agagaagatc tgggcccggg
      121 agatcctgga ctcccgcggg aaccccacag tggaggtgga tctctatact gccaaaggtc
      181 ttttccgggc tgcagtgccc agtggagcct ctacgggcat ctatgaggcc ctggagctga
      241 gggatggaga caaacagcgt tacttaggca aaggtgtcct gaaggcagtg gaccacatca
      301 actccaccat cgcgccagcc ctcatcagct caggtctctc tgtggtggag caagagaaac
      361 tggacaacct gatgctggag ttggatggga ctgagaacaa atccaagttt ggggccaatg
      421 ccatcctggg tgtgtctctg gccgtgtgta aggcaggggc agctgagcgg gaactgcccc
      481 tgtatcgcca cattgctcag ctggccggga actcagacct catcctgcct gtgccggcct
      541 tcaacgtgat caatggtggc tctcatgctg gcaacaagct ggccatgcag gagttcatga
      601 tcctcccagt gggagctgag agctttcggg atgccatgcg actaggtgca gaggtctacc
      661 atacactcaa gggagtcatc aaggacaaat acggcaagga tgccaccaat gtgggggatg
      721 aaggtggctt tgcccccaat atcctggaga acagtgaagc cttggagctg gtgaaggaag
      781 ccatcgacaa ggctggctac acggaaaaga tcgttattgg catggatgtt gctgcctcag
      841 agttttatcg tgatggcaaa tatgacttgg acttcaagtc tcccactgat ccttcccgat
      901 acatcactgg ggaccagctg ggggcactct accaggactt tgtcagggac tatcctgtgg
      961 tctccattga ggacccattt gaccaggatg attgggctgc ctggtccaag ttcacagcca
     1021 atgtagggat ccagattgtg ggtgatgacc tgacagtgac caacccaaaa cgtattgagc
     1081 gggcagtgga agaaaaggcc tgcaactgtc tgctgctcaa ggtcaaccag atcggctcgg
     1141 tcactgaagc catccaagcg tgcaagctgg cccaggagaa tggctggggg gtcatggtga
     1201 gtcatcgctc aggagagact gaggacacat tcattgctga cctggtggtg gggctgtgca
     1261 caggccagat caagactggt gccccgtgcc gttctgaacg tctggctaaa tacaaccagc
     1321 tcatgagaat tgaggaagag ctgggggatg aagctcgctt tgccggacat aacttccgta
     1381 atcccagtgt gctgtgattc ctctgcttgc ctggagacgt ggaacctctg tctcatcctc
     1441 ctggaacctt gctgtcctga tctgtgatag ttcaccccct gagatcccct gagccccagg
     1501 gtgcccagaa cttccctgat tgacctgctc cgctgctcct tggcttacct gacctcttgc
     1561 tgtctctgct cgccctcctt tctgtgccct actcattggg gttccgcact ttccacttct
     1621 tcctttctct ttctctcttc cctcagaaac tagaaatgtg aatgaggatt attataaaag
     1681 ggggtccgtg gaagaatgat cagcatctgt gatgggagcg tcagggttgg tgtgctgagg
     1741 tgttagagag ggaccatgtg tcacttgtgc tttgctcttg tcccacgtgt cttccacttt
     1801 gcatatgagc cgtgaactgt gcatagtgct gggatggagg ggagtgttgg gcatgtgatc
     1861 acgcctggct aataaggctt tagtgtattt atttatttat ttattttatt tgtttttcat
     1921 tcatcccatt aatcatttcc ccataactca atggcctaaa actggcctga cttgggggaa
     1981 cgatgtgtct gtatttcatg tggctgtaga tcccaagatg actggggtgg gaggtcttgc
     2041 tagaatggga agggtcatag aaagggcctt gacatcagtt cctttgtgtg tactcactga
     2101 agcctgcgtt ggtccagagc ggaggctgtg tgcctggggg agttttcctc tatacatctc
     2161 tccccaaccc taggttccct gttcttcctc cagctgcacc agagcaacct ctcactcccc
     2221 atgccacgtt ccacagttgc caccacctct gtggcattga aatgagcacc tccattaaag
     2281 tctgaatcag tgca
//
