LOCUS       NM_018212              13092 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens ENAH actin regulator (ENAH), transcript variant 2,
            mRNA.
ACCESSION   NM_018212 NM_145240 XM_351323
VERSION     NM_018212.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13092)
  AUTHORS   Yin,X., Wang,J., Shen,Z., Jia,Q., Bian,Y., Yang,Z., Liu,Y., Li,Y.
            and Zhang,H.
  TITLE     ENAH transcriptionally activated by YY1 promotes growth and
            invasion of laryngocarcinoma cells through PI3K/AKT signaling
  JOURNAL   Eur J Pharmacol 983, 176991 (2024)
   PUBMED   39265883
  REMARK    GeneRIF: ENAH transcriptionally activated by YY1 promotes growth
            and invasion of laryngocarcinoma cells through PI3K/AKT signaling.
REFERENCE   2  (bases 1 to 13092)
  AUTHORS   Cui,H., Liang,X., Zhu,Y., Elayah,S.A., Qi,H., Xie,L., Guo,Z.,
            Siya,F., Ming,Y., Yuxin,G., Tu,J. and Na,S.
  TITLE     Mena as a key enhancer factor of EMT to promote metastasis of human
            tongue squamous cell carcinoma
  JOURNAL   Oral Dis 30 (4), 2084-2096 (2024)
   PUBMED   37203597
  REMARK    GeneRIF: Mena as a key enhancer factor of EMT to promote metastasis
            of human tongue squamous cell carcinoma.
REFERENCE   3  (bases 1 to 13092)
  AUTHORS   He,L., She,C., Jiang,S., Qi,Z., Deng,Z., Ji,L., Cui,Y. and Wu,J.
  TITLE     Mammalian enabled protein enhances tamoxifen sensitivity of the
            hormone receptor-positive breast cancer patients by suppressing the
            AKT signaling pathway
  JOURNAL   Biol Direct 19 (1), 21 (2024)
   PUBMED   38459605
  REMARK    GeneRIF: Mammalian enabled protein enhances tamoxifen sensitivity
            of the hormone receptor-positive breast cancer patients by
            suppressing the AKT signaling pathway.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 13092)
  AUTHORS   Liu,R., Miao,K., Gao,L. and He,L.
  TITLE     TRIM67 interacts with ENAH to regulate the apoptosis and autophagy
            of lung cancer cells
  JOURNAL   Gen Physiol Biophys 43 (2), 163-173 (2024)
   PUBMED   38477606
  REMARK    GeneRIF: TRIM67 interacts with ENAH to regulate the apoptosis and
            autophagy of lung cancer cells.
REFERENCE   5  (bases 1 to 13092)
  AUTHORS   Wang,W., Taufalele,P.V., Millet,M., Homsy,K., Smart,K.,
            Berestesky,E.D., Schunk,C.T., Rowe,M.M., Bordeleau,F. and
            Reinhart-King,C.A.
  TITLE     Matrix stiffness regulates tumor cell intravasation through
            expression and ESRP1-mediated alternative splicing of MENA
  JOURNAL   Cell Rep 42 (4), 112338 (2023)
   PUBMED   37027295
  REMARK    GeneRIF: Matrix stiffness regulates tumor cell intravasation
            through expression and ESRP1-mediated alternative splicing of MENA.
REFERENCE   6  (bases 1 to 13092)
  AUTHORS   Urbanelli,L., Massini,C., Emiliani,C., Orlacchio,A., Bernardi,G.
            and Orlacchio,A.
  TITLE     Characterization of human Enah gene
  JOURNAL   Biochim Biophys Acta 1759 (1-2), 99-107 (2006)
   PUBMED   16494957
REFERENCE   7  (bases 1 to 13092)
  AUTHORS   Mammoto,A., Sasaki,T., Asakura,T., Hotta,I., Imamura,H.,
            Takahashi,K., Matsuura,Y., Shirao,T. and Takai,Y.
  TITLE     Interactions of drebrin and gephyrin with profilin
  JOURNAL   Biochem Biophys Res Commun 243 (1), 86-89 (1998)
   PUBMED   9473484
REFERENCE   8  (bases 1 to 13092)
  AUTHORS   Ermekova,K.S., Zambrano,N., Linn,H., Minopoli,G., Gertler,F.,
            Russo,T. and Sudol,M.
  TITLE     The WW domain of neural protein FE65 interacts with proline-rich
            motifs in Mena, the mammalian homolog of Drosophila enabled
  JOURNAL   J Biol Chem 272 (52), 32869-32877 (1997)
   PUBMED   9407065
REFERENCE   9  (bases 1 to 13092)
  AUTHORS   Niebuhr,K., Ebel,F., Frank,R., Reinhard,M., Domann,E., Carl,U.D.,
            Walter,U., Gertler,F.B., Wehland,J. and Chakraborty,T.
  TITLE     A novel proline-rich motif present in ActA of Listeria
            monocytogenes and cytoskeletal proteins is the ligand for the EVH1
            domain, a protein module present in the Ena/VASP family
  JOURNAL   EMBO J 16 (17), 5433-5444 (1997)
   PUBMED   9312002
REFERENCE   10 (bases 1 to 13092)
  AUTHORS   Sazuka,T., Tomooka,Y., Kathju,S., Ikawa,Y., Noda,M. and Kumar,S.
  TITLE     Identification of a developmentally regulated gene in the mouse
            central nervous system which encodes a novel proline rich protein
  JOURNAL   Biochim Biophys Acta 1132 (3), 240-248 (1992)
   PUBMED   1420303
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB961874.1, AY345143.1,
            AK096246.1, AC092811.2 and BU675547.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_018212.5.
            
            Summary: This gene encodes a member of the enabled/
            vasodilator-stimulated phosphoprotein. Members of this gene family
            are involved in actin-based motility. This protein is involved in
            regulating the assembly of actin filaments and modulates cell
            adhesion and motility. Alternate splice variants of this gene have
            been correlated with tumor invasiveness in certain tissues and
            these variants may serve as prognostic markers. A pseudogene of
            this gene is found on chromosome 3. [provided by RefSeq, Sep 2016].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (b) has the same
            N- and C-termini but is shorter compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.1944490.1, AY345143.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000366843.7/ ENSP00000355808.2
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-548               CB961874.1         47-594
            549-2378            AY345143.1         473-2302
            2379-4443           AK096246.1         1695-3759
            4444-13089          AC092811.2         36140-44785
            13090-13092         BU675547.1         16-18               c
FEATURES             Location/Qualifiers
     source          1..13092
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q42.12"
     gene            1..13092
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /note="ENAH actin regulator"
                     /db_xref="GeneID:55740"
                     /db_xref="HGNC:HGNC:18271"
                     /db_xref="MIM:609061"
     exon            1..438
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             434..2146
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /note="isoform b is encoded by transcript variant 2;
                     protein enabled homolog; Mena delta 11a"
                     /codon_start=1
                     /product="protein enabled homolog isoform b"
                     /protein_id="NP_060682.2"
                     /db_xref="CCDS:CCDS31040.1"
                     /db_xref="GeneID:55740"
                     /db_xref="HGNC:HGNC:18271"
                     /db_xref="MIM:609061"
                     /translation="MSEQSICQARAAVMVYDDANKKWVPAGGSTGFSRVHIYHHTGNN
                     TFRVVGRKIQDHQVVINCAIPKGLKYNQATQTFHQWRDARQVYGLNFGSKEDANVFAS
                     AMMHALEVLNSQETGPTLPRQNSQLPAQVQNGPSQEELEIQRRQLQEQQRQKELERER
                     LERERMERERLERERLERERLERERLEQEQLERERQERERQERLERQERLERQERLER
                     QERLDRERQERQERERLERLERERQERERQEQLEREQLEWERERRISSAAAPASVETP
                     LNSVLGDSSASEPGLQAASQPAETPSQQGIVLGPLAPPPPPPLPPGPAQASVALPPPP
                     GPPPPPPLPSTGPPPPPPPPPLPNQVPPPPPPPPAPPLPASGFFLASMSEDNRPLTGL
                     AAAIAGAKLRKVSRMEDTSFPSGGNAIGVNSASSKTDTGRGNGPLPLGGSGLMEEMSA
                     LLARRRRIAEKGSTIETEQKEDKGEDSEPVTSKASSTSTPEPTRKPWERTNTMNGSKS
                     PVISRPKSTPLSQPSANGVQTEGLDYDRLKQDILDEMRKELTKLKEELIDAIRQELSK
                     SNTA"
     exon            439..604
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            605..782
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            783..867
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            868..1235
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1346
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1651
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..1797
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1798..1855
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1856..1904
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..1971
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1972..2050
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2051..2108
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2109..13092
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5087..5092
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /note="hexamer: AATAAA"
     polyA_site      5107
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /note="major polyA site"
     polyA_site      5290
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /note="major polyA site"
     regulatory      5629..5634
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /note="hexamer: TATAAA"
     polyA_site      5653
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
     regulatory      5918..5923
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /note="hexamer: ATTAAA"
     polyA_site      5943
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
     regulatory      7061..7066
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /note="hexamer: GATAAA"
     polyA_site      7096
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
     regulatory      13065..13070
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
                     /note="hexamer: AATAAA"
     polyA_site      13092
                     /gene="ENAH"
                     /gene_synonym="ENA; MENA; NDPP1"
ORIGIN      
        1 gactctcctc ccgcggctgc agcagctgcc ggttgcacac ggcctcggcg gcggcgcggc
       61 gagcgcgggc ctgtggagcg gcggccgggc gcgcggcccc ggcgggcacc cctcagaggg
      121 cggccgagga agctccggga ggaggagcag gaccacgagg aggtgggagg cggcggccgc
      181 ctggggacca gctccgcgcc tcggcctctc cgccccctcc ccagcctttc tctcgccctc
      241 ttctcccaca ctcccggccg gcgcctcggc tttgtgcgag gagatggtgt agccccctgg
      301 ccgccgaagg aggagccgga cacttgtctc ccgtctccga gctgctcccc acccctggag
      361 gagagacccc cccctcggct cggcgccttc tgcgtctccc ggctggtggg gaagcctctg
      421 cgccgccggc accatgagtg aacagagtat ctgtcaggca agagctgctg tgatggttta
      481 tgatgatgcc aataagaagt gggtgccagc tggtggctca actggattca gcagagttca
      541 tatctatcac catacaggca acaacacatt cagagtggtg ggcaggaaga ttcaggacca
      601 tcaggtcgtg ataaactgtg ccattcctaa agggttgaag tacaatcaag ctacacagac
      661 cttccaccag tggcgagatg ctagacaggt gtatggtctc aactttggca gcaaagagga
      721 tgccaatgtc ttcgcaagtg ccatgatgca tgccttagaa gtgttaaatt cacaggaaac
      781 agggccaaca ttgcctagac aaaactcaca actacctgct caagttcaaa atggcccatc
      841 ccaagaagaa ttggaaattc aaagaagaca actacaagaa cagcaacggc aaaaggagct
      901 ggagcgggaa aggctggagc gagaaagaat ggaaagagaa aggttggaga gagagaggtt
      961 agaaagggaa aggctggaga gggagcgact ggaacaagaa cagctggaga gagagagaca
     1021 agaacgggaa cggcaggaac gcctggagcg gcaggaacgc ctggagcggc aggaacgcct
     1081 ggagcggcag gaacgcctgg atcgggagag gcaagaaaga caagaacgag agaggctgga
     1141 gagactggaa cgggagaggc aagaaaggga gcgacaagag cagttagaaa gggaacagct
     1201 ggaatgggag agagagcgca gaatatcaag tgctgctgcc cctgcctctg ttgagactcc
     1261 tctaaactct gtgctgggag actcttctgc ttctgagcca ggcttgcagg cagcctctca
     1321 gccggccgag actccatccc aacagggcat tgtcttggga ccacttgcac ctccacctcc
     1381 tccaccactc ccaccagggc ctgcacaggc ttcagtagcc ctccctcctc ccccagggcc
     1441 ccctccacct cctccactcc catccaccgg gcctccaccg ccccctcctc cccctcctct
     1501 ccctaatcaa gtaccccctc ctcctccacc acctcctgcc ccacccctcc ctgcatctgg
     1561 attctttttg gcatccatgt cagaagacaa tcgcccttta actggacttg cagctgcaat
     1621 tgccggagca aaacttagga aagtgtcacg gatggaggat acctctttcc caagtggagg
     1681 gaatgctatt ggtgtgaact ccgcctcatc taaaacagat acaggccgtg gaaatggacc
     1741 ccttccttta gggggtagtg gtttaatgga agaaatgagt gccctgctgg ccaggaggag
     1801 aagaattgct gaaaagggat caacaataga aacagaacaa aaagaggaca aaggtgaaga
     1861 ttcagagcct gtaacttcta aggcctcttc aacaagtaca cctgaaccaa caagaaaacc
     1921 ttgggaaaga acaaatacaa tgaatggcag caagtcacct gttatctcca gaccaaaatc
     1981 cacaccctta tcacagccca gtgccaatgg agtccagacg gaaggacttg actatgacag
     2041 gctgaagcag gacattttag atgaaatgag aaaagaatta acaaagctaa aagaagagct
     2101 cattgatgca atcaggcagg aactgagcaa gtcaaatact gcatagagga acagactaag
     2161 gagagatagg actttaatct ggaggaaaaa tatcctacaa acaacaactg ttcacaacag
     2221 caaaccccta catttatgag ctgtaagaag aaaatggaga caaacagaag gagggaaaaa
     2281 ccaacctact ctgaaagcct tcagacatta tgactctggt gataagctct ttccctctcc
     2341 gtttgctgct tttttctggc ctttacaaca gaatggaaga gaatcattta agagttcctg
     2401 taacagttat gcagaaaata ctaaaaccca tcaggcaaga tcaccacgca ttgaaatatt
     2461 ttcatatcaa gataaagtcg cacattttcc acaatacatt gctaaaataa agaggagaaa
     2521 ggcttaggaa gtttttttgc agagagtgct ggtaaagaat tgagcaagtt tgctattgta
     2581 ttgtaatgtt tctctcaggt ttgttcttcc tatcatgttt gatattccat gaataattga
     2641 gatcagccct atgtaagtta agatcataat atgtggaaca aatggaattg taagtgcttt
     2701 caaagggtaa tatttataag aaagtgtccg aaaaatgttt cttcagcttg agaaatttta
     2761 gaatgatagg aagtttctcg agttagcctt catgcaattt tgtagattaa aacataaaat
     2821 ttgtccagaa cttaaagatt tagatgcctt cctaaattgt tacaatgctt taccaaatct
     2881 atgacttcta cataacacaa accagtggtc aaatgtaaac actatattgt agatttactg
     2941 taggttttca acctttttta gatttatgca tgtggacatt tttataatgt aattacaatc
     3001 accacaaggt tagctttttt aattgcagac agtaatgcat gtcacactaa tatgtagtgg
     3061 ccttttcaag gcctagtccc agggaaaaca ttttgtagag tataggggag tgggaggaag
     3121 gggaggaata attttttatt taaagttgat ttctgcacta tctttttctc agttacctgc
     3181 atgaataaat aatgagaaat attttgtgac tttaattggt aaatatgtta caaaaccaag
     3241 tacttaatct tttacatcat gtcttcagct atttgtattt taaccagtaa tttcaatggt
     3301 ctgaaacatg attctgagct tcacataata tcttaactgt ggaactcaaa agtttgatca
     3361 ctgaatttgg cagttattat tacctaggta cccccgctgt tacacaggtg tttagatacg
     3421 tgttcctgaa tgaagctgct tttgaatttt gttatgttga aatgcaagaa ataacaatga
     3481 tggcagcaat taaggtcaca gaaatcatta ggtaaaggaa aaccaatgag gagttctgca
     3541 gttttctttt aataagtaaa gtgagacttg ggtggtggga agaaggaagg tgggaagaag
     3601 gaattagaca ctctgcctgc cactctgcgt gtgtgtgctc tcgcgcacgt gctgtctata
     3661 tggaagccac tcccttttct ttcctttgaa actggtaagg ttaaaatagg ggagaaatcc
     3721 tacatgttgg aatgatagct ttttggaaaa tttaagaaac tctccaggct ctccatcttg
     3781 atttatgctt gagttgttat gtgccatatt tgctttgaac tctgattatc agaagtttta
     3841 ctaaaacttt gaaataattc actttcatct gctttctaga ttttgtacat ctcagtccat
     3901 aaagcaaagc ttgttgatag tgtagttttc taaacgctgc aaatttgcag cctttaccac
     3961 tacaaagaag tttggatgag ggattttttt tttctttgtc aaaatagttc ctgtttctgt
     4021 agaaatttca tttttagatt aaactgtgat ggatgagcta tcataattca agtatacatt
     4081 tcttttttct atcagatatt cattgtcatg cagtagtagt aaaaacatca aagatgcagc
     4141 aagcttatta agtattattt tctaaaagaa ataggaggca ttttcatctt tattattgta
     4201 cttttggtta tgcaaacact ttgataatat aaacagttat gtcccctata aatctggtca
     4261 gcaacctctt ttgattttgt tgggtaagtt aaatagtctg tagtaggtag agtactgggt
     4321 acaagtggtc caaactaaga taagagacta aaataaaatg ctaaatctta aaagaaactg
     4381 ggtttatgca ctaaacgttt tgtgccttgg tctaatatta acatgatgta tgtgtaaact
     4441 gacaaaaaaa aaaaaaaaaa aaaaccagtg ttgaatcatg ctatatttcc catcatccca
     4501 gattgctaaa tgttctttcc aagaagtctg aatgaattat tatatacatt tgctatcata
     4561 catgacagtt gtgtcattat tagagatttc agtaattaaa atgggaaaca gaagcctaag
     4621 ttatttacct tatcaaaacc acgttccttg gaaccacatt attatgatgg tttttgtgct
     4681 cttgtacgtt ggatgtctta agctgagtac agtttagggg atcctttcta atgacaggaa
     4741 ggcactgctt tcctcaacac tgtgatctga cctgtgacaa gtctgtacta tccactatgt
     4801 aaatggctca caagtcaatt gcaaacaaac tgaatgtaca aatcttagtg ctggtgctga
     4861 aatctcttca gaatagtcat catgatttaa aagtttgttt aaaaatttgc aagcatcaag
     4921 tgtcaatcaa aactgaagat gaaacactaa tgaatgttga attctcatgc ttttaggtgt
     4981 acttcctttt tagaattttc agttttctgc tatttttacc gtaactattt cgtttaaatt
     5041 tgtttcattt aagtttccta cacgctaact tcgtttgtgc gattgaaata aaattgcagt
     5101 atatatatgt tgcttgtttg tgacacttag ataatcttct gaaataattt gttttaaagt
     5161 aagttatatt ggatgtgaaa aacacctttt aaagtccaag actttactac ttaaaagttt
     5221 acatgtcacg tcactattct ttacagattt catagaagtc gtgaatacta agtaatgtat
     5281 acaaaagaaa tatgtgatga tgtgtctact ttttgtttga aataactgtt gggtgttccc
     5341 tcttcttatt ttctctctgc ccaccttgac acagtaactt cctcttatag tcaagatgat
     5401 gattaagtgt ttaattttta ttccattttg ttctgcaaca cgttcaggta cttaaaactc
     5461 attcatgcag ttagaattat cctttatgta gtttgcatgg taaaggacat tgtgtggtaa
     5521 agggcattat tgagtaatga attttaagta tagcaaaata tgtatagtgt ttatacatga
     5581 tgctgatttc ttaaaaccta catataagca gtagtacttt ttctctatta taaagtatta
     5641 attttgagtt ccattttata attctgatgt cttcattaga aatacaaaaa tacaatcctt
     5701 gtattttttc tttgaccaga cccaaatttt ccccttcccc cctccattct cctctccact
     5761 tctcgtggac cgcgtgttcc caatattcat taacatttgc agccattaca gagttacagt
     5821 tgtaaaattt tcagctgttt tttttttttt ttttttgctc tagcctgtct catgttcttg
     5881 cttctcttca tatgtaagtc agtatttttg tctacaaatt aaaagtcact tgtcattcaa
     5941 aaatgagtta agtaacatga taatctttta aatttaccta gctacaactt ttatgtctct
     6001 tgtaaattta tatatacttt cagtgccatt gtgaagaatt ggccttttaa ttctaagtag
     6061 tattacccta tgaagcacag tgtgcaaagg tatgctacaa ggtctcttta attcctgtgt
     6121 ggccattata attttataac accattaccc tttaaattct accgattata agcagcgtaa
     6181 aagtaactat ataaagcaaa catcgcaaag gaactctgca ggagctctta attcctttat
     6241 gtagctatca taaaattcac tttcctgaag acatttactc tcattcactt ccaaactcca
     6301 aacctttttc tggtagcacc acttttgttt ttaatagaaa gatgagttca tatctgtaca
     6361 tctctccaaa gctctaagga atgagaaaag gatcctagta tattgaaatt actgatgttt
     6421 aatacctctg ccttttcact aaaagccatt taatattttt aaagtcaaaa cttgacatac
     6481 aggtatttat aaggaatctc catgactctg aaggaatgaa attgatgtag gtagctttgg
     6541 ctatgtaaag acatagtaga ggacaattac ttaaagaaga gttttctttt gaggatttgt
     6601 agatttgact aagcagctga agtcagtaac ccctcaagaa agtccgttta tagaagccaa
     6661 gaactgttgg aggtctgtga tgcaggtttt tctgttccca acatcaaaag ctgtggatta
     6721 tttggtgtgt tcccctttat gttatctctc gataattttt gagacatcaa gattttttaa
     6781 aatatcaata ctggaaagat gaaaccttca aattaaaacc taaaattgat tagaaagaaa
     6841 agtaaaatat taatgcagta aatttaggat ggttacagat actcaccatc aacataaata
     6901 aatggagttc taggataaat tatttgtgga atactaatgg tcaagtcaca taagagtaaa
     6961 atcttttggt gaggagacac atttgacaac tgctaaagaa ttaactaaat ttgtctttct
     7021 aaatagtttt ctttataaag taacagaaac attgtaaaag gataaatgac tgtcataatg
     7081 acttaggatg ttgtaagaat gtctgccaca tttattggtt atttctgttg aaatctgaag
     7141 gaatcgatac atctgaaaat tgagaaaggg aaataggact gtccccgtta cagatactct
     7201 gtgatgcaca caaccttcta aacggaagtg ttagtatcat ttaaagcaca tagtaggcct
     7261 tgagtatttg cagattaaca tgcagaaaaa atcaaagatg aagccccaat taagtgtttg
     7321 aaccaaagaa tcacagtcac tgagctgcac ccccgttctg tgaagcatgt taaatactca
     7381 taaagagcag agagcagggc ccgtagacgc ttctggtgta ggacacagga attctaggca
     7441 gagggagaaa attaggggcc ccagttggtg gctgagagta aaaagaatat accatttgag
     7501 tagtgctgtt ctttttcctc agtttttaaa gacatataat tcctagtagt taggacatct
     7561 aaatagctcg gtaaactggt cagaggcggg aggattgctt gaggccagaa gttttgagat
     7621 cagcctgggt aacagggaga ctcatttcta caaaaaaatt taaaaattta ggccaggcat
     7681 ggtggtgcac acctgtaatc ccagctactc aggagactga agcgggagga tcacctgagc
     7741 ccagggagga cgcggctgca gtgagccgtt atcatgccac tgtactccag cctgggtgac
     7801 agagcgagaa cctgtctcta aaaaaaaaaa aaaaaaattc taagagaata tagcttttca
     7861 tagaaattat tagaaaagct tacatatttc attagagagc ccagtttgtt agactacatt
     7921 tattccgtaa aagttctata tacaaatgac aacccatagt ttatcatgta aatctgattt
     7981 aactgttccc ttcaattcca agaaagattt cttcagcagt agatgacaat tctaaaagca
     8041 taaagaaata actaataaag gagatggatg atgttatagg ttggtttggt tgggttttag
     8101 taaatagtta ttcttctaag aaggcttaga aaatcgagaa ttgaatagtt ggtactttga
     8161 attccatagg aaaaagcact cagaagaaaa agtctacatt ggctagtgtt tagattgaga
     8221 gttaattttc ccatcaagtc caaaagtttc catccaagtc tatagaaact gagagaaaag
     8281 gttcttggct tacacttaaa atacacattg ttgcaaccat ataagagaga tacattacag
     8341 aacgctttta aaaatttggt ctcttaagcg cctaacagtc catgctttag tcacatctgt
     8401 gaaacctctt ttcttttttt ctttttcttt tatttttttt tttaaagatt aaaggcattt
     8461 taaaaataaa tgtttcatgg ccgggcatgg tggctcacgc ctgtaatccc agcactttgg
     8521 gaggctgagg tgggcagatc acctgaggtc aggagtttga gaccagcctg gccaacatga
     8581 tgaaacccca tctctactaa aaatacaaaa aaattggctg ggcatagtgg tgggcacctg
     8641 taatcccagc tcttcgggag gctgagacag aagagtcgct tgaatccagg aggtggaggt
     8701 tgcagtgagc taagaccgcg ccactgtact ccagcctggg cgacagagcg agattccatc
     8761 tctaaataaa taaatgtttt tcaacgtgtt ttatgtgaga aataccagct tttcattatt
     8821 actaccagtt gatctcaggt gtcacttggt tggttctgct gaccgaggca ctgggcattt
     8881 taaatatgga gtttccctcc acccattgat caagtctgag gtttgtccat ccttggtggc
     8941 aggctagctt aaccatggat tcacaaaccc catttgattg tcgtaagtca taaggccacc
     9001 ccagtgccag tggttgggca tcacagtgag tctgaggtga tgaatttgag ttagctgtag
     9061 gctagttccg caactcagca atagcccatg gatggttggt aaaagacgtt gactttgcca
     9121 cttaaatttc caggagaaga atagcttata gtttatctga ggaaagtaat tgggaaggct
     9181 tagatattga gaagaaagcc ctgttaaaat ctaggtagca tttcctttct tctttattac
     9241 tagagggatg ggaggttcag gcctgagtca ggactttaca atttggtgtc cttcccagag
     9301 gtactttggg cttgatcatt ttaagtttca attgcaaaaa aaaatttttg acggagtgtt
     9361 gctctgtcac tcaggttgga gtgcagtggt gcgatctcgg ctcactgcaa cttccccccc
     9421 ctgggttcaa agggattttc ctgcctcagc ctcccaagta gctgggacta caggcgcgtg
     9481 ccaccatgcc cgactaattt ttgtattttt agtagagatg gggtttcacc atgttgccca
     9541 ggctggtctt gaactcctga gctcaggtga tctgcccgcc tcggccttca attgcagaag
     9601 tttttgaagg gagaatattt gatcctcaca taaaatgtcc ttgtttctga aagaaaacat
     9661 ggtaaaaatt gttattgagt ccatgtcttt gccaccttgt tggacttgat ttccctttct
     9721 ctgtgttcct cccgccctca gtctctctca gtctgtcctt ttttgtgtgt ggagttgtgg
     9781 gcaggtcatt ggactgggac tgactttacc agttttcagg ctgtggggct agtcagtcat
     9841 tctctctgag ccttctacct ctaaagttga gttctttttt taaattcttt ttgttttgtt
     9901 tttatttttt ccttgccact atacaagcaa ggatagataa agctttttat tattattgtt
     9961 attattatta tttcgagaca gtctcgctct gtcatccagg ctggagtgca gtggtgtgat
    10021 ttcagctcac tgcaacctct gcctcccggg ttcaagcgat tctcctacct cagcctcctt
    10081 agtagctggg actacaggca tgcgccacca cacccggcta atttttgtat ttttagtaga
    10141 gacagggttt caccgtattg gccaggctgg tctcgaactc ctgacctcag gtgatccacc
    10201 caccttggcc tcccaaagtg ttgggattac aggcataagc gaccgtgccc atccccgata
    10261 aagttgagtt ctgaatttcc tctttcactg tattctcggc agctactggt tggtttattc
    10321 ttttttttta atcttttttg gtttattctt cttttgatct ttgaagagaa ggagaaaaaa
    10381 gatctgagtt tggctctccc atcttaagtg cagcttttag ttgttttata gtcatagacg
    10441 ctattgcact ggacagcatg catcctggat gtaaacaatg cggaaaaatc aatggagtga
    10501 ccatcttggg gtcagtggtg ttgcataaag caccagtggt gtgctgttct gtgtcagttt
    10561 cctctgcgcc acgtgctttc acagtttgaa ttaccagcat ctctgtggtg gcttcagtac
    10621 attctccatt gtgcttcaac ctggaagcac attctgccgt tcagaaagac tgattccaat
    10681 catgtaatgg gaccacacga gatccatgct ccaattatat gaatagcaga aggtccattt
    10741 gactcttgta gttcaagagc tgtttctaag taaacagact ttccagagct cgatgaagtg
    10801 aagaagccct tctttcaatt cctgggcaga attcagtggt tttcatatca tctgaacctg
    10861 ctgtgctgct gtttattcta tcactgtcag ccttgaaagg aaaaaaggaa taatcttacc
    10921 ctcctgccct tcttatttta tatcttccat cttatccatc tatatccatg tagtcattcc
    10981 ttccgagttc cctggtttta atccaaaaga ctctgtttaa cagcaatgtc ttggcagacc
    11041 aggtgccgct tccgtgccat ttgagggctc tgagtctttg cttcgtctta ggaagataaa
    11101 gataatttgg cagaaagtca gagatggaaa tagcagtttg ggatgataaa agatgggtcc
    11161 tgttttgctg acattctttt actttggttc ttggtgcgtg tcctttggga ttcatttctt
    11221 gctagcatga ataggacatg cttttcgtta ggtgcttttc ttttttggag aacatgaagt
    11281 gtttctaaca aaactgaatg agctagaaag aattctattt tgagacatct gtagttgatg
    11341 caaagtattt ctgtaatcct caatgacagt acttacatat ttgcatagaa tttttttttt
    11401 aatttttgag gaaacctctc tggagtctgt atttatagga gcatcctgaa agtccctatt
    11461 atgtagctgg tgggagcgag agagggacag ccacactcgt gctgtgagca gagcacaact
    11521 tcaggcagag tgaatgcctt ctgagttctc attggaagct ttctgttgct cagcctctgg
    11581 cagatttcag aaccttctac ttaagccagg tgtggtggtg cgtgctccta ggcacagcga
    11641 ctcggggggc tgaggcagaa ggatcacttg agcccaaagt tggaggccag cctgggcaac
    11701 atagcgaggc ctgtctcctt aaaaaataaa taaataaata ataaataaat aaataaataa
    11761 aaaccttgtc cttgactatt gtgctgtctg ctgccccttt gtagggatgg ggttggggtc
    11821 agaataagca tgtgcatgaa tggcggaaag ctttggtggt gtttgtttgt ttgtttgttt
    11881 tttgagaggt gggggagtca gcagtgaacc tttctggtta aagaacaacg tacataattt
    11941 ctattatgag atttagaagg ttaaatactt ttttttttca ggcctaccaa gttgatagac
    12001 caaaagtata tagtagaaca gcacagtaga aaaaatttaa aagtgaattt cttatggtcc
    12061 aaaaggatgg atagtcacag tccataacct ttcataatat atgctggaat tggaatctct
    12121 tgtcatcttg ctttgctctg aattttagaa ctagaagtgt gcatgtatgt caaaagatta
    12181 tgcagagcta ttttctttgc taacaatgta aacatttgca ccccatgaat gggactttat
    12241 aatctggaag tgagcctaag gttgcgtata ctgaagtcat gttttaaaag ttaaggttga
    12301 cttaaacata tacaaggctg tattttaatt gtaactagaa atcatttact atgcatacag
    12361 aacctcatgt atcttatcat ggaatttgtg aattaatggg ccttgccgaa ttcaagggta
    12421 agggaaactg gtaacaactt gttttaaatc aacatactat aagctcctgc ttgcttgact
    12481 caagttgttt gaaaatctga attaaacagg atctcattgt caggaaacat cttctaagct
    12541 tgacatctac gtttaagaag ggaacgtgga agagaaggtg agacatgaaa ctaaaacagc
    12601 tgggagggta ggatcagact gcacctgatt tttggaccaa agtttcatat aggaataaat
    12661 aaaaaggagg accacttgtg agccaaccta ggaaggactt gccagaaagt agcagctcct
    12721 gctgcaaaat acagtgcctt caaaatgatt ctctgtgtga attgcatgca atttcatgta
    12781 accataaagt gtaacctgta tgtgcatgac atgtgtctag aatgtgtaaa gttaggattc
    12841 agcaggaagg ggcctggccc tgtcactgtg gtggatcagt ggacctgctg aagtcactgg
    12901 actcatgtga cttaggtttg cagcatcaga cccctgtgtt tgcattaagt ggccacatcg
    12961 ttgaaatcgg atcaatctct ccctcagctt tctttcctac tttgcagctt tgctggtttt
    13021 aactgcttca ttcttctgct tcttggtatc cttttttctt ttgaaataaa aacatgaaat
    13081 acttaattca aa
//
