LOCUS       NM_001427               3395 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens engrailed homeobox 2 (EN2), mRNA.
ACCESSION   NM_001427
VERSION     NM_001427.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Li,Y., Jiang,J., Wang,X., Cao,Y., Tang,L., Song,X., Huang,F.,
            Li,M., Chen,F., Wan,H. and Ye,S.
  TITLE     Engrailed 2 serves as a master regulator of the super-enhancer in
            the TNC gene locus in non-small cell lung cancer
  JOURNAL   Environ Toxicol 39 (3), 1442-1455 (2024)
   PUBMED   37987507
  REMARK    GeneRIF: Engrailed 2 serves as a master regulator of the
            super-enhancer in the TNC gene locus in non-small cell lung cancer.
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Lee,Y., Dho,S.H., Lee,J., Hwang,J.H., Kim,M., Choi,W.Y., Lee,J.Y.,
            Lee,J., Chang,W., Lee,M.Y., Choi,J., Kim,T.Y. and Kim,L.K.
  TITLE     Hypermethylation of PDX1, EN2, and MSX1 predicts the prognosis of
            colorectal cancer
  JOURNAL   Exp Mol Med 54 (2), 156-168 (2022)
   PUBMED   35169223
  REMARK    GeneRIF: Hypermethylation of PDX1, EN2, and MSX1 predicts the
            prognosis of colorectal cancer.
REFERENCE   3  (bases 1 to 3395)
  AUTHORS   Xie,L. and Pan,Z.
  TITLE     Circular RNA circ_0000467 regulates colorectal cancer development
            via miR-382-5p/EN2 axis
  JOURNAL   Bioengineered 12 (1), 886-897 (2021)
   PUBMED   33685351
  REMARK    GeneRIF: Circular RNA circ_0000467 regulates colorectal cancer
            development via miR-382-5p/EN2 axis.
REFERENCE   4  (bases 1 to 3395)
  AUTHORS   Li,Y., Liu,J., Xiao,Q., Tian,R., Zhou,Z., Gan,Y., Li,Y., Shu,G. and
            Yin,G.
  TITLE     EN2 as an oncogene promotes tumor progression via regulating CCL20
            in colorectal cancer
  JOURNAL   Cell Death Dis 11 (7), 604 (2020)
   PUBMED   32732864
  REMARK    GeneRIF: EN2 as an oncogene promotes tumor progression via
            regulating CCL20 in colorectal cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3395)
  AUTHORS   Li,Q., Shi,Y., Sa,R., Hao,J., Hu,J., Xiao,M., Wang,C., Yan,L.,
            Qiao,B. and Chen,G.
  TITLE     Altered staining patterns and expression level of Engrailed-2 in
            benign prostatic hyperplasia and prostate Cancer predict prostatic
            disease progression
  JOURNAL   BMC Cancer 20 (1), 555 (2020)
   PUBMED   32539763
  REMARK    GeneRIF: Altered staining patterns and expression level of
            Engrailed-2 in benign prostatic hyperplasia and prostate Cancer
            predict prostatic disease progression.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3395)
  AUTHORS   Kozmik,Z., Sure,U., Ruedi,D., Busslinger,M. and Aguzzi,A.
  TITLE     Deregulated expression of PAX5 in medulloblastoma
  JOURNAL   Proc Natl Acad Sci U S A 92 (12), 5709-5713 (1995)
   PUBMED   7777574
REFERENCE   7  (bases 1 to 3395)
  AUTHORS   Logan,C., Hanks,M.C., Noble-Topham,S., Nallainathan,D.,
            Provart,N.J. and Joyner,A.L.
  TITLE     Cloning and sequence comparison of the mouse, human, and chicken
            engrailed genes reveal potential functional domains and regulatory
            regions
  JOURNAL   Dev Genet 13 (5), 345-358 (1992)
   PUBMED   1363401
REFERENCE   8  (bases 1 to 3395)
  AUTHORS   Joyner,A.L., Herrup,K., Auerbach,B.A., Davis,C.A. and Rossant,J.
  TITLE     Subtle cerebellar phenotype in mice homozygous for a targeted
            deletion of the En-2 homeobox
  JOURNAL   Science 251 (4998), 1239-1243 (1991)
   PUBMED   1672471
REFERENCE   9  (bases 1 to 3395)
  AUTHORS   Poole,S.J., Law,M.L., Kao,F.T. and Lau,Y.F.
  TITLE     Isolation and chromosomal localization of the human En-2 gene
  JOURNAL   Genomics 4 (3), 225-231 (1989)
   PUBMED   2565873
REFERENCE   10 (bases 1 to 3395)
  AUTHORS   Logan,C., Willard,H.F., Rommens,J.M. and Joyner,A.L.
  TITLE     Chromosomal localization of the human homeo box-containing genes,
            EN1 and EN2
  JOURNAL   Genomics 4 (2), 206-209 (1989)
   PUBMED   2567700
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008060.5 and H16179.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_001427.3.
            
            Summary: Homeobox-containing genes are thought to have a role in
            controlling development. In Drosophila, the 'engrailed' (en) gene
            plays an important role during development in segmentation, where
            it is required for the formation of posterior compartments.
            Different mutations in the mouse homologs, En1 and En2, produced
            different developmental defects that frequently are lethal. The
            human engrailed homologs 1 and 2 encode homeodomain-containing
            proteins and have been implicated in the control of pattern
            formation during development of the central nervous system.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.106245.1,
                                           SRR1660807.97961.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2142680
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000297375.4/ ENSP00000297375.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-934               AC008060.5         88973-89906
            935-3381            AC008060.5         93215-95661
            3382-3395           H16179.1           11-24               c
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q36.3"
     gene            1..3395
                     /gene="EN2"
                     /note="engrailed homeobox 2"
                     /db_xref="GeneID:2020"
                     /db_xref="HGNC:HGNC:3343"
                     /db_xref="MIM:131310"
     exon            1..934
                     /gene="EN2"
                     /inference="alignment:Splign:2.1.0"
     CDS             250..1251
                     /gene="EN2"
                     /note="engrailed homolog 2; engrailed-2; hu-En-2; homeobox
                     protein en-2"
                     /codon_start=1
                     /product="homeobox protein engrailed-2"
                     /protein_id="NP_001418.2"
                     /db_xref="CCDS:CCDS5940.1"
                     /db_xref="GeneID:2020"
                     /db_xref="HGNC:HGNC:3343"
                     /db_xref="MIM:131310"
                     /translation="MEENDPKPGEAAAAVEGQRQPESSPGGGSGGGGGSSPGEADTGR
                     RRALMLPAVLQAPGNHQHPHRITNFFIDNILRPEFGRRKDAGTCCAGAGGGRGGGAGG
                     EGGASGAEGGGGAGGSEQLLGSGSREPRQNPPCAPGAGGPLPAAGSDSPGDGEGGSKT
                     LSLHGGAKKGGDPGGPLDGSLKARGLGGGDLSVSSDSDSSQAGANLGAQPMLWPAWVY
                     CTRYSDRPSSGPRSRKPKKKNPNKEDKRPRTAFTAEQLQRLKAEFQTNRYLTEQRRQS
                     LAQELSLNESQIKIWFQNKRAKIKKATGNKNTLAVHLMAQGLYNHSTTAKEGKSDSE"
     exon            935..3395
                     /gene="EN2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1413..1418
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EN2"
                     /note="hexamer: TATAAA"
     polyA_site      1441
                     /gene="EN2"
                     /note="major polyA site"
     regulatory      1662..1667
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EN2"
                     /note="hexamer: AATAAA"
     polyA_site      1688
                     /gene="EN2"
     regulatory      3371..3376
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EN2"
                     /note="hexamer: AACAAA"
     polyA_site      3395
                     /gene="EN2"
                     /note="major polyA site"
ORIGIN      
        1 tctctcatcg tctgggcgag cggggcggct cgtggtgttt ctaacccagt tcgtggattc
       61 aaaggtggct ccgcgccgag cgcggccggc gacttgtagg acctcagccc tggccgcggc
      121 cgccgcgcac gccctcggaa gactcggcgg ggtgggggcg cgggggtctc cgtgtgcgcc
      181 gcgggagggc cgaaggctga tttggaaggg cgtccccgga gaaccagtgt gggatttact
      241 gtgaacagca tggaggagaa tgaccccaag cctggcgaag cagcggcggc ggtggaggga
      301 cagcggcagc cggaatccag ccccggcggc ggctcgggcg gcggcggcgg tagcagcccg
      361 ggcgaagcgg acaccgggcg ccggcgggct ctgatgctgc ccgcggtcct gcaggcgccc
      421 ggcaaccacc agcacccgca ccgcatcacc aacttcttca tcgacaacat cctgcggccc
      481 gagttcggcc ggcgaaagga cgcggggacc tgctgtgcgg gcgcgggagg aggaaggggc
      541 ggcggagccg gcggcgaagg cggcgcgagc ggtgcggagg gaggcggcgg cgcgggcggc
      601 tcggagcagc tcttgggctc gggctcccga gagccccggc agaacccgcc atgtgcgccc
      661 ggcgcgggcg ggccgctccc agccgccggc agcgactctc cgggtgacgg ggaaggcggc
      721 tccaagacgc tctcgctgca cggtggcgcc aagaaaggcg gcgaccccgg cggccccctg
      781 gacgggtcgc tcaaggcccg cggcttgggc ggcggcgacc tgtcggtgag ctcggactcg
      841 gacagctcgc aagccggcgc caacctgggc gcgcagccca tgctctggcc ggcgtgggtc
      901 tactgtacgc gctactcgga ccggccttct tcaggtccca ggtctcgaaa accaaagaag
      961 aagaacccga acaaagagga caagcggccg cgcacggcct ttaccgccga gcagctgcag
     1021 aggctcaagg ccgagttcca gaccaacagg tacctgacgg agcagcggcg ccagagcctg
     1081 gcgcaggagc tgagcctcaa cgagtcacag atcaagattt ggttccagaa caagcgcgcc
     1141 aagatcaaga aggccacggg caacaagaac acgctggccg tgcacctcat ggcacagggc
     1201 ttgtacaacc actccaccac agccaaggag ggcaagtcgg acagcgagta gggcgggggg
     1261 catggaggcc aggtctcagt ccgcgctaaa caatgcaata atttaaaatc ataaagggcc
     1321 agtgtataaa gattatacca gcattaatag tgaaaatatt gtgtattagc taaggttctg
     1381 aaatattcta tgtatatatc atttacaggt ggtataaaat ccaaaatatc tgactataaa
     1441 atattttttt gagttttttg tgtttatgag attatgctaa ttttatgggt ttttttcttt
     1501 tttgcgaagg gggctgctta gggtttcacc tttttttaat cccctaagct ccattatatg
     1561 acattggaca cttttttatt attccaaaag aagaaaaaat taaaacaact tgctgaagtc
     1621 caaagatttt ttattgctgc atttcacaca actgtgaacc gaataaatag ctcctatttg
     1681 gtctatgact tctgccactt tgtttgtgtt ggcttggtga ggacagcagg aggggcccac
     1741 acctcaagcc tggaccagcc acctcaaggc cttggggagc ttaggggacc tggtgggaga
     1801 gaggggactt ccagggtcct tgggccagtt ctgggatttg gccctgggaa gcagcccagc
     1861 gtaccccagg cctgctctgg gaagtcggct ccatgctcac cagcagccgc ccaggcccgc
     1921 agcctcaccc ggctccctct cctcaccctc ctgcacctaa ctccctcctc cttctccttt
     1981 ttcctcctct tcctccttcc tccttcctcc tgctcctcct ttcttcttct ttttcttctc
     2041 ctcctcctcc ttccttcctc ctcctccttc tctttcctcc tcctcctcac caagggccca
     2101 accgtgtgca tacatcgtct gcgtctgtgg tctgtgtcgc tgtccccagt cccaccgcag
     2161 tcctgccgca ggcctaaccc tcctgccctg ggcactgcct ccatgcagaa gcgcttcgag
     2221 gttctggggc taaaggcctg gggtgtgtgg cctaaagccc aagagcggtg gggcgaccct
     2281 ccttttggct tggccccagg aatttcctgt gactccacca gccatcatgg gtgccagcca
     2341 gggtcccaga aatgaggcca tggctcactg tttctgggcg ggcagaaggc tctgtagagg
     2401 gagatggcat catctatctt cctttccttt ttcttttctt ccctattttt ttcttttttt
     2461 cctttatttt tttcttttct tggagtggct gcttctgcta tagagaacat tcttccaaga
     2521 taaatatgtg tgtttacaca tatgtctgca tgcatgtgaa cacacacaca cacacacaca
     2581 caccaggcgt gtttgagtcc acagttctga aacatgtggc taccttgtct ttcaaaagaa
     2641 ctcagaatcc tccaggatct agaagaagga agaaagtgtg taaataatca tttcttatca
     2701 tcactttttg tcttttcttg ttttttaaaa tatacatttt atttttgaag gtgtggtaca
     2761 gtgtaaatta aatatattca atatatttcc caccaagtac ctatatatgt atataaacaa
     2821 acacattatc tatatataac gccacactgt cttctgttta gtgtatgggg aaagaccaat
     2881 ccaactgtcc atctgtggct gggacagccc agggggtgtg cccacggctg acccaggggt
     2941 gtgcacacgg ctgagctggg agtcccgctg gtctccctga ggactgaggg tgaacttcgc
     3001 tctttgcctt aaacctcttt atttcattgc agtaatagtt ttacgttgta cataatagtg
     3061 taaacctttt taaaaaggaa agtataaaaa caaaagttgt aatttaaaag tctgaataac
     3121 catctgctgc ttaggaaact caatgaaatg acatgccttt ttagcaggaa gcaaagttgg
     3181 tttctgtttt ttgttttctt tgttgtttta gtttataaaa catgtgcatt ttacagttcc
     3241 agtatcaaat atttataatc ttatgagaaa tgaatgaatg tttctattta caactgtgct
     3301 tatcaaaatt gtgaacaccc ccacccccgc atttttgtgt gttgaaattc ttgaaggtta
     3361 cattaaataa aacaaaatct ctttattata aaata
//
