LOCUS       NM_019063               5546 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens EMAP like 4 (EML4), transcript variant 1, mRNA.
ACCESSION   NM_019063
VERSION     NM_019063.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Gonzalez-Martinez,D., Roth,L., Mumford,T.R., Guan,J., Le,A.,
            Doebele,R.C., Huang,B., Tulpule,A., Niewiadomska-Bugaj,M.,
            Bivona,T.G. and Bugaj,L.J.
  TITLE     Oncogenic EML4-ALK assemblies suppress growth factor perception and
            modulate drug tolerance
  JOURNAL   Nat Commun 15 (1), 9473 (2024)
   PUBMED   39488530
  REMARK    GeneRIF: Oncogenic EML4-ALK assemblies suppress growth factor
            perception and modulate drug tolerance.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Cordier,F., Van der Meulen,J., van Roy,N., De Wilde,J., van
            Dijck,H., Vanhoenacker,F., Lambrechts,M., Noyez,V., Van de
            Vijver,K., Ferdinande,L., Dendooven,A., Van Dorpe,J. and
            Creytens,D.
  TITLE     Malignant pleural mesothelioma with an EML4-ALK fusion: Expect the
            unexpected!
  JOURNAL   Pathol Res Pract 231, 153772 (2022)
   PUBMED   35074700
  REMARK    GeneRIF: Malignant pleural mesothelioma with an EML4-ALK fusion:
            Expect the unexpected!
REFERENCE   3  (bases 1 to 5546)
  AUTHORS   Wang,S., Luo,R., Shi,Y. and Han,X.
  TITLE     The impact of the ALK fusion variant on clinical outcomes in
            EML4-ALK patients with NSCLC: a systematic review and meta-analysis
  JOURNAL   Future Oncol 18 (3), 385-402 (2022)
   PUBMED   34783600
  REMARK    GeneRIF: The impact of the ALK fusion variant on clinical outcomes
            in EML4-ALK patients with NSCLC: a systematic review and
            meta-analysis.
REFERENCE   4  (bases 1 to 5546)
  AUTHORS   Sampson,J., Richards,M.W., Choi,J., Fry,A.M. and Bayliss,R.
  TITLE     Phase-separated foci of EML4-ALK facilitate signalling and depend
            upon an active kinase conformation
  JOURNAL   EMBO Rep 22 (12), e53693 (2021)
   PUBMED   34661367
  REMARK    GeneRIF: Phase-separated foci of EML4-ALK facilitate signalling and
            depend upon an active kinase conformation.
REFERENCE   5  (bases 1 to 5546)
  AUTHORS   Shinmura,K., Kageyama,S., Tao,H., Bunai,T., Suzuki,M., Kamo,T.,
            Takamochi,K., Suzuki,K., Tanahashi,M., Niwa,H., Ogawa,H. and
            Sugimura,H.
  TITLE     EML4-ALK fusion transcripts, but no NPM-, TPM3-, CLTC-, ATIC-, or
            TFG-ALK fusion transcripts, in non-small cell lung carcinomas
  JOURNAL   Lung Cancer 61 (2), 163-169 (2008)
   PUBMED   18242762
  REMARK    GeneRIF: EML4-ALK fusion gene product is involved in the
            carcinogenesis in non-small cell lung carcinomas
REFERENCE   6  (bases 1 to 5546)
  AUTHORS   Inamura,K., Takeuchi,K., Togashi,Y., Nomura,K., Ninomiya,H.,
            Okui,M., Satoh,Y., Okumura,S., Nakagawa,K., Soda,M., Choi,Y.L.,
            Niki,T., Mano,H. and Ishikawa,Y.
  TITLE     EML4-ALK fusion is linked to histological characteristics in a
            subset of lung cancers
  JOURNAL   J Thorac Oncol 3 (1), 13-17 (2008)
   PUBMED   18166835
  REMARK    GeneRIF: EML4 is part of a fusion protein linked to histological
            characteristics in a subset of lung neoplasms.
REFERENCE   7  (bases 1 to 5546)
  AUTHORS   Rikova,K., Guo,A., Zeng,Q., Possemato,A., Yu,J., Haack,H.,
            Nardone,J., Lee,K., Reeves,C., Li,Y., Hu,Y., Tan,Z., Stokes,M.,
            Sullivan,L., Mitchell,J., Wetzel,R., Macneill,J., Ren,J.M.,
            Yuan,J., Bakalarski,C.E., Villen,J., Kornhauser,J.M., Smith,B.,
            Li,D., Zhou,X., Gygi,S.P., Gu,T.L., Polakiewicz,R.D., Rush,J. and
            Comb,M.J.
  TITLE     Global survey of phosphotyrosine signaling identifies oncogenic
            kinases in lung cancer
  JOURNAL   Cell 131 (6), 1190-1203 (2007)
   PUBMED   18083107
REFERENCE   8  (bases 1 to 5546)
  AUTHORS   Soda,M., Choi,Y.L., Enomoto,M., Takada,S., Yamashita,Y.,
            Ishikawa,S., Fujiwara,S., Watanabe,H., Kurashina,K., Hatanaka,H.,
            Bando,M., Ohno,S., Ishikawa,Y., Aburatani,H., Niki,T., Sohara,Y.,
            Sugiyama,Y. and Mano,H.
  TITLE     Identification of the transforming EML4-ALK fusion gene in
            non-small-cell lung cancer
  JOURNAL   Nature 448 (7153), 561-566 (2007)
   PUBMED   17625570
REFERENCE   9  (bases 1 to 5546)
  AUTHORS   Pollmann,M., Parwaresch,R., Adam-Klages,S., Kruse,M.L., Buck,F. and
            Heidebrecht,H.J.
  TITLE     Human EML4, a novel member of the EMAP family, is essential for
            microtubule formation
  JOURNAL   Exp Cell Res 312 (17), 3241-3251 (2006)
   PUBMED   16890222
  REMARK    GeneRIF: EML4 negative cells showed a completely modified
            microtubule network, indicating that EML4 is necessary for correct
            microtubule formation.
REFERENCE   10 (bases 1 to 5546)
  AUTHORS   Heidebrecht,H.J., Buck,F., Pollmann,M., Siebert,R. and
            Parwaresch,R.
  TITLE     Cloning and localization of C2orf2(ropp120), a previously unknown
            WD repeat protein
  JOURNAL   Genomics 68 (3), 348-350 (2000)
   PUBMED   10995578
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC083949.3, AC096668.1 and
            AC006038.3.
            
            On Nov 23, 2018 this sequence version replaced NM_019063.4.
            
            Summary: This gene is a member of the echinoderm microtubule
            associated protein-like family. The encoded WD-repeat protein may
            be involved in microtubule formation. Abnormal fusion of parts of
            this gene with portions of the anaplastic lymphoma receptor
            tyrosine kinase gene, which generates EML4-ALK fusion transcripts,
            is one of the primary mutations associated with non-small cell lung
            cancer. Alternative splicing of this gene results in two transcript
            variants. [provided by RefSeq, Jan 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF177377.2, SRR1660805.69091.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000318522.10/ ENSP00000320663.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-284               AC083949.3         97471-97754
            285-467             AC096668.1         66326-66508
            468-597             AC006038.3         3352-3481
            598-771             AC006038.3         7972-8145
            772-900             AC006038.3         10029-10157
            901-926             AC006038.3         11557-11582
            927-1050            AC006038.3         27701-27824
            1051-1200           AC006038.3         29674-29823
            1201-1270           AC006038.3         31485-31554
            1271-1381           AC006038.3         33120-33230
            1382-1477           AC006038.3         35078-35173
            1478-1612           AC006038.3         41976-42110
            1613-1748           AC006038.3         42232-42367
            1749-1900           AC006038.3         48092-48243
            1901-2026           AC006038.3         49955-50080
            2027-2158           AC006038.3         50166-50297
            2159-2226           AC006038.3         51335-51402
            2227-2315           AC006038.3         62813-62901
            2316-2413           AC006038.3         64278-64375
            2414-2501           AC006038.3         72318-72405
            2502-2600           AC006038.3         73005-73103
            2601-2731           AC006038.3         75737-75867
            2732-5546           AC006038.3         76585-79399
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p21"
     gene            1..5546
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /note="EMAP like 4"
                     /db_xref="GeneID:27436"
                     /db_xref="HGNC:HGNC:1316"
                     /db_xref="MIM:607442"
     exon            1..284
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     CDS             260..3205
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /note="isoform a is encoded by transcript variant 1;
                     restrictedly overexpressed proliferation-associated
                     protein; ropp 120; echinoderm microtubule associated
                     protein like 4; EML4/ALK fusion protein"
                     /codon_start=1
                     /product="echinoderm microtubule-associated protein-like 4
                     isoform a"
                     /protein_id="NP_061936.3"
                     /db_xref="CCDS:CCDS1807.1"
                     /db_xref="GeneID:27436"
                     /db_xref="HGNC:HGNC:1316"
                     /db_xref="MIM:607442"
                     /translation="MDGFAGSLDDSISAASTSDVQDRLSALESRVQQQEDEITVLKAA
                     LADVLRRLAISEDHVASVKKSVSSKGQPSPRAVIPMSCITNGSGANRKPSHTSAVSIA
                     GKETLSSAAKSGTEKKKEKPQGQREKKEESHSNDQSPQIRASPSPQPSSQPLQIHRQT
                     PESKNATPTKSIKRPSPAEKSHNSWENSDDSRNKLSKIPSTPKLIPKVTKTADKHKDV
                     IINQEGEYIKMFMRGRPITMFIPSDVDNYDDIRTELPPEKLKLEWAYGYRGKDCRANV
                     YLLPTGKIVYFIASVVVLFNYEERTQRHYLGHTDCVKCLAIHPDKIRIATGQIAGVDK
                     DGRPLQPHVRVWDSVTLSTLQIIGLGTFERGVGCLDFSKADSGVHLCIIDDSNEHMLT
                     VWDWQKKAKGAEIKTTNEVVLAVEFHPTDANTIITCGKSHIFFWTWSGNSLTRKQGIF
                     GKYEKPKFVQCLAFLGNGDVLTGDSGGVMLIWSKTTVEPTPGKGPKGVYQISKQIKAH
                     DGSVFTLCQMRNGMLLTGGGKDRKIILWDHDLNPEREIEVPDQYGTIRAVAEGKADQF
                     LVGTSRNFILRGTFNDGFQIEVQGHTDELWGLATHPFKDLLLTCAQDRQVCLWNSMEH
                     RLEWTRLVDEPGHCADFHPSGTVVAIGTHSGRWFVLDAETRDLVSIHTDGNEQLSVMR
                     YSIDGTFLAVGSHDNFIYLYVVSENGRKYSRYGRCTGHSSYITHLDWSPDNKYIMSNS
                     GDYEILYWDIPNGCKLIRNRSDCKDIDWTTYTCVLGFQVFGVWPEGSDGTDINALVRS
                     HNRKVIAVADDFCKVHLFQYPCSKAKAPSHKYSAHSSHVTNVSFTHNDSHLISTGGKD
                     MSIIQWKLVEKLSLPQNETVADTTLTKAPVSSTESVIQSNTPTPPPSQPLNETAEEES
                     RISSSPTLLENSLEQTVEPSEDHSEEESEEGSGDLGEPLYEEPCNEISKEQAKATLLE
                     DQQDPSPSS"
     exon            285..467
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            468..597
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            598..771
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            772..900
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            901..926
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1050
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1200
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1270
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1381
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1477
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..1612
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1748
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..1900
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            1901..2026
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..2158
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            2159..2226
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            2227..2315
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            2316..2413
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            2414..2501
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            2502..2600
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2731
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     exon            2732..5546
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5515..5520
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /note="hexamer: AATAAA"
     regulatory      5520..5525
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /note="hexamer: AATAAT"
     polyA_site      5543
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
     polyA_site      5546
                     /gene="EML4"
                     /gene_synonym="C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120"
                     /note="major polyA site"
ORIGIN      
        1 gcggcgctcg cggctgctgc ctgggaggga ggccgggcag gcggctgagc ggcgcggctc
       61 tcaacgtgac ggggaagtgg ttcgggcggc cgcggcttac taccccaggg cgaacggacg
      121 gacgacggag gcgggagccg gtagccgagc cgggcgacct agagaacgag cgggtcaggc
      181 tcagcgtcgg ccactctgtc ggtccgctga atgaagtgcc cgcccctcta agcccggagc
      241 ccggcgcttt ccccgcaaga tggacggttt cgccggcagt ctcgatgata gtatttctgc
      301 tgcaagtact tctgatgttc aagatcgcct gtcagctctt gagtcacgag ttcagcaaca
      361 agaagatgaa atcactgtgc taaaggcggc tttggctgat gttttgaggc gtcttgcaat
      421 ctctgaagat catgtggcct cagtgaaaaa atcagtctca agtaaaggcc aaccaagccc
      481 tcgagcagtt attcccatgt cctgtataac caatggaagt ggtgcaaaca gaaaaccaag
      541 tcataccagt gctgtctcaa ttgcaggaaa agaaactctt tcatctgctg ctaaaagtgg
      601 tacagaaaaa aagaaagaaa aaccacaagg acagagagaa aaaaaagagg aatctcattc
      661 taatgatcaa agtccacaaa ttcgagcatc accttctccc cagccctctt cacaacctct
      721 ccaaatacac agacaaactc cagaaagcaa gaatgctact cccaccaaaa gcataaaacg
      781 accatcacca gctgaaaagt cacataattc ttgggaaaat tcagatgata gccgtaataa
      841 attgtcgaaa ataccttcaa cacccaaatt aataccaaaa gttaccaaaa ctgcagacaa
      901 gcataaagat gtcatcatca accaagaagg agaatatatt aaaatgttta tgcgcggtcg
      961 gccaattacc atgttcattc cttccgatgt tgacaactat gatgacatca gaacggaact
     1021 gcctcctgag aagctcaaac tggagtgggc atatggttat cgaggaaagg actgtagagc
     1081 taatgtttac cttcttccga ccgggaaaat agtttatttc attgcatcag tagtagtact
     1141 atttaattat gaggagagaa ctcagcgaca ctacctgggc catacagact gtgtgaaatg
     1201 ccttgctata catcctgaca aaattaggat tgcaactgga cagatagctg gcgtggataa
     1261 agatggaagg cctctacaac cccacgtcag agtgtgggat tctgttactc tatccacact
     1321 gcagattatt ggacttggca cttttgagcg tggagtagga tgcctggatt tttcaaaagc
     1381 agattcaggt gttcatttat gtattattga tgactccaat gagcatatgc ttactgtatg
     1441 ggactggcag aagaaagcaa aaggagcaga aataaagaca acaaatgaag ttgttttggc
     1501 tgtggagttt cacccaacag atgcaaatac cataattaca tgcggtaaat ctcatatttt
     1561 cttctggacc tggagcggca attcactaac aagaaaacag ggaatttttg ggaaatatga
     1621 aaagccaaaa tttgtgcagt gtttagcatt cttggggaat ggagatgttc ttactggaga
     1681 ctcaggtgga gtcatgctta tatggagcaa aactactgta gagcccacac ctgggaaagg
     1741 acctaaaggt gtatatcaaa tcagcaaaca aatcaaagct catgatggca gtgtgttcac
     1801 actttgtcag atgagaaatg ggatgttatt aactggagga gggaaagaca gaaaaataat
     1861 tctgtgggat catgatctga atcctgaaag agaaatagag gttcctgatc agtatggcac
     1921 aatcagagct gtagcagaag gaaaggcaga tcaattttta gtaggcacat cacgaaactt
     1981 tattttacga ggaacattta atgatggctt ccaaatagaa gtacagggtc atacagatga
     2041 gctttggggt cttgccacac atcccttcaa agatttgctc ttgacatgtg ctcaggacag
     2101 gcaggtgtgc ctgtggaact caatggaaca caggctggaa tggaccaggc tggtagatga
     2161 accaggacac tgtgcagatt ttcatccaag tggcacagtg gtggccatag gaacgcactc
     2221 aggcaggtgg tttgttctgg atgcagaaac cagagatcta gtttctatcc acacagacgg
     2281 gaatgaacag ctctctgtga tgcgctactc aatagatggt accttcctgg ctgtaggatc
     2341 tcatgacaac tttatttacc tctatgtagt ctctgaaaat ggaagaaaat atagcagata
     2401 tggaaggtgc actggacatt ccagctacat cacacacctt gactggtccc cagacaacaa
     2461 gtatataatg tctaactcgg gagactatga aatattgtac tgggacattc caaatggctg
     2521 caaactaatc aggaatcgat cggattgtaa ggacattgat tggacgacat atacctgtgt
     2581 gctaggattt caagtatttg gtgtctggcc agaaggatct gatgggacag atatcaatgc
     2641 actggtgcga tcccacaata gaaaggtgat agctgttgcc gatgactttt gtaaagtcca
     2701 tctgtttcag tatccctgct ccaaagcaaa ggctcccagt cacaagtaca gtgcccacag
     2761 cagccatgtc accaatgtca gttttactca caatgacagt cacctgatat caactggtgg
     2821 aaaagacatg agcatcattc agtggaaact tgtggaaaag ttatctttgc ctcagaatga
     2881 gactgtagcg gatactactc taaccaaagc ccccgtctct tccactgaaa gtgtcatcca
     2941 atctaatact cccacaccgc ctccttctca gcccttaaat gagacagctg aagaggaaag
     3001 tagaataagc agttctccca cacttctgga gaacagcctg gaacaaactg tggagccaag
     3061 tgaagaccac agcgaggagg agagtgaaga gggcagcgga gaccttggtg agcctcttta
     3121 tgaagagcca tgcaacgaga taagcaagga gcaggccaaa gccacccttc tggaggacca
     3181 gcaagaccct tcgccctcgt cctaacaccc tggcttcagt gcaactcttt tccttcagct
     3241 gcatgtgatt ttgtgataaa gttcaggtaa caggatgggc agtgatggag aatcactgtt
     3301 gattgagatt ttggtttcca tgtgatttgt tttcttcaat agtcttattt tcagtctctc
     3361 aaatacagcc aacttaaagt tttagtttgg tgtttattga aaattaacca aacttaatac
     3421 taggagaaga ctgaatcatt aatgatgtct cacaaattac tgtgtaccta agtggtgtga
     3481 tgtaaatact ggaaacaaaa acagcagttg cattgatttt gaaaacaaac ccccttgtta
     3541 tctgaacatg ttttcttcag gaacaaccag aggtatcaca aacactgtta ctcatctact
     3601 ggctcagact gtactacttt tttttttttt tttcctgaaa aagaaaccag aaaaaaatgt
     3661 actcttactg agataccctc tcaccccaaa tgtgtaatgg aaaattttta attaagaaaa
     3721 acttcagttt tgccaagtgc aatggtgttg ccttctttaa aaaatgccgt tttcttacac
     3781 taccagtgga tgtccagaca tgctcttagt ctactagaga ggtgctgcct tttctaagtc
     3841 ataatgagga acagtccctt aatttcttgt gtgcaactct gttttatcct agaactaaga
     3901 gagcattggt ttgttaaaga gctttcaatg tatattaaaa ccttcaatac tcagaaatga
     3961 tggattcctc caaggagtcc tttactagcc taaacattct caaatgtttg agattcaagt
     4021 gaatggaagg aaaaccacat gcctttaaaa ctaaactgta ataattacct ggctaatttc
     4081 agctaagcct tcatcataat ttgttccctc agtaatagga gaaatataaa tacagtaagt
     4141 ttagattatt gaattggtgc ttgaaattta ttggttttgt tgtaatttta tacagattat
     4201 atgagggata agatactcat caaattgcaa attctttttt ttacagaagt gtgggtaaca
     4261 gtcacagcag ttttttttac caacagcata cttaacagac ttgctgtgta gcagtttttt
     4321 tctggtggag ttgctgtaag tcttgtaagt ctaatgtggc tatcctactc ttttgggcaa
     4381 tgcatgtatt atgcattgga aaggtatttt ttttaagttc tgttggctag ctatggtttt
     4441 cagtacattt cctactttaa gagtaattac tgacaaatat gtatttccta tatgtttata
     4501 ctttgattat aaaaaagtat tttgttttga ttttttaact tgctgcattg ttttgatact
     4561 ttctattttt ttggtcaaat catgtttaga aactttggat gagttaagaa gtcttaagta
     4621 tgcaggcgtt tacgtgattg tgccattcca aagtgcatca gaactgtcat tcccttctaa
     4681 tatcttctca ggagtaatac aaatcaggta tttcatcatc atttggtaat atgaaaactc
     4741 cagtgaactc ccaaggacat ttacaacatt tatattcaca cgctgtatgg aagggtgtgg
     4801 gtgtgtgtga aggggcgagt ggagacactg tgtgtatctc tagataagaa gatatgcacc
     4861 acgttgaaaa tactcagtgt agatctctat gtgtataggt atctgtatat ctttcctttt
     4921 gtttacaact gttaaaaaac ctcaaaatag ttctcttcaa aagaagagag attccaagca
     4981 acccatcttt cttcagtatg tatgttctgt acatacttat cggagcgcgc cagtaagtat
     5041 caggcatata tatctgtctg ttagcaatga ttattacatc atcagatcag catgtgctat
     5101 actccctgca agaaatatac tgacatgaac aggcagttct tggagaagaa agagcatttc
     5161 tttaagtacc tggggaatac agctctcagt gatcagcagg gagtttattt gaggacatca
     5221 gtcacctttg gggttgccat gtacaatgag atttataatc atgatactct tcggtggtag
     5281 tttcaaaaga cactactaat acgcaggaag cgttccagct atttaatgct ggcaactact
     5341 gtttaatggt cagttaaatc tgtgataatg gttggaagtg ggtggggtta tgaaattgta
     5401 gatgttttta gaaaaacttg tgaatgaaaa tgaatccaag tgtttcatgt gaagatgttg
     5461 agccattgct atcatgcatt cctgtctcat ggcagaaaat tttgaagatt aaaaaataaa
     5521 ataatcaaaa tgtttcctct ttctaa
//
