LOCUS       NM_153265               3266 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens EMAP like 3 (EML3), transcript variant 3, mRNA.
ACCESSION   NM_153265
VERSION     NM_153265.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Luo,J., Yang,B., Xin,G., Sun,M., Zhang,B., Guo,X., Jiang,Q. and
            Zhang,C.
  TITLE     The microtubule-associated protein EML3 regulates mitotic spindle
            assembly by recruiting the Augmin complex to spindle microtubules
  JOURNAL   J Biol Chem 294 (14), 5643-5656 (2019)
   PUBMED   30723163
  REMARK    GeneRIF: results indicate that EML3 regulates mitotic spindle
            assembly and the kinetochore-MT connection by regulating MT-based
            MT nucleation and recruiting Augmin/gamma-TuRC to MTs.
REFERENCE   3  (bases 1 to 3266)
  AUTHORS   Richards,M.W., O'Regan,L., Roth,D., Montgomery,J.M., Straube,A.,
            Fry,A.M. and Bayliss,R.
  TITLE     Microtubule association of EML proteins and the EML4-ALK variant 3
            oncoprotein require an N-terminal trimerization domain
  JOURNAL   Biochem J 467 (3), 529-536 (2015)
   PUBMED   25740311
REFERENCE   4  (bases 1 to 3266)
  AUTHORS   Tegha-Dunghu,J., Neumann,B., Reber,S., Krause,R., Erfle,H.,
            Walter,T., Held,M., Rogers,P., Hupfeld,K., Ruppert,T., Ellenberg,J.
            and Gruss,O.J.
  TITLE     EML3 is a nuclear microtubule-binding protein required for the
            correct alignment of chromosomes in metaphase
  JOURNAL   J Cell Sci 121 (Pt 10), 1718-1726 (2008)
   PUBMED   18445686
  REMARK    GeneRIF: Functional analysis of EML3 demonstrates an important role
            for EML3 in correct metaphase chromosome alignment.
REFERENCE   5  (bases 1 to 3266)
  AUTHORS   Jin,J., Smith,F.D., Stark,C., Wells,C.D., Fawcett,J.P.,
            Kulkarni,S., Metalnikov,P., O'Donnell,P., Taylor,P., Taylor,L.,
            Zougman,A., Woodgett,J.R., Langeberg,L.K., Scott,J.D. and Pawson,T.
  TITLE     Proteomic, functional, and domain-based analysis of in vivo 14-3-3
            binding proteins involved in cytoskeletal regulation and cellular
            organization
  JOURNAL   Curr Biol 14 (16), 1436-1450 (2004)
   PUBMED   15324660
REFERENCE   6  (bases 1 to 3266)
  AUTHORS   O'Connor,V., Houtman,S.H., De Zeeuw,C.I., Bliss,T.V. and
            French,P.J.
  TITLE     Eml5, a novel WD40 domain protein expressed in rat brain
  JOURNAL   Gene 336 (1), 127-137 (2004)
   PUBMED   15225882
REFERENCE   7  (bases 1 to 3266)
  AUTHORS   Pozuelo Rubio,M., Geraghty,K.M., Wong,B.H., Wood,N.T.,
            Campbell,D.G., Morrice,N. and Mackintosh,C.
  TITLE     14-3-3-affinity purification of over 200 human phosphoproteins
            reveals new links to regulation of cellular metabolism,
            proliferation and trafficking
  JOURNAL   Biochem J 379 (Pt 2), 395-408 (2004)
   PUBMED   14744259
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP001458.6, CN386216.1, CN386217.1, BC108280.1 and BC050467.1.
            
            On Jun 2, 2019 this sequence version replaced NM_153265.2.
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            splice junction at the 5' end of an exon and differs in the 3' UTR
            and coding sequence compared to variant 1. The resulting isoform
            (3) lacks a single alternate aa and has a shorter and distinct
            C-terminus compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC108280.1, SRR11853560.5411.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000394773.7/ ENSP00000378254.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AP001458.6         20033-20042         c
            11-129              CN386216.1         1-119
            130-226             CN386217.1         111-207
            227-527             BC108280.1         1-301
            528-3265            BC050467.1         104-2841
            3266-3266           AP001458.6         9485-9485           c
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.3"
     gene            1..3266
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /note="EMAP like 3"
                     /db_xref="GeneID:256364"
                     /db_xref="HGNC:HGNC:26666"
                     /db_xref="MIM:618118"
     exon            1..340
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     CDS             319..3009
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /note="isoform 3 is encoded by transcript variant 3;
                     EMAP-3; echinoderm microtubule associated protein like 3"
                     /codon_start=1
                     /product="echinoderm microtubule-associated protein-like 3
                     isoform 3"
                     /protein_id="NP_694997.2"
                     /db_xref="CCDS:CCDS8023.2"
                     /db_xref="GeneID:256364"
                     /db_xref="HGNC:HGNC:26666"
                     /db_xref="MIM:618118"
                     /translation="MDGAAGPGDGPAREALQSLSQRLRVQEQEMELVKAALAEALRLL
                     RLQVPPSSLQGSGTPAPPGDSLAAPPGLPPTCTPSLVSRGTQTETEVELKSSPGPPGL
                     SNGPPAPQGASEEPSGTQSEGGGSSSSGAGSPGPPGILRPLQPPQRADTPRRNSSSSS
                     SPSERPRQKLSRKAISSANLLVRSGSTESRGGKDPLSSPGGPGSRRSNYNLEGISVKM
                     FLRGRPITMYIPSGIRSLEELPSGPPPETLSLDWVYGYRGRDSRSNLFVLRSGEVVYF
                     IACVVVLYRPGGGPGGPGGGGQRHYRGHTDCVRCLAVHPDGVRVASGQTAGVDKDGKP
                     LQPVVHIWDSETLLKLQEIGLGAFERGVGALAFSAADQGAFLCVVDDSNEHMLSVWDC
                     SRGMKLAEIKSTNDSVLAVGFNPRDSSCIVTSGKSHVHFWNWSGGVGVPGNGTLTRKQ
                     GVFGKYKKPKFIPCFVFLPDGDILTGDSEGNILTWGRSPSDSKTPGRGGAKETYGIVA
                     QAHAHEGSIFALCLRRDGTVLSGGGRDRRLVQWGPGLVALQEAEIPEHFGAVRAIAEG
                     LGSELLVGTTKNALLRGDLAQGFSPVIQGHTDELWGLCTHPSQNRFLTCGHDRQLCLW
                     DGESHALAWSIDLKETGLCADFHPSGAVVAVGLNTGRWLVLDTETREIVSDVIDGNEQ
                     LSVVRYSPDGLYLAIGSHDNVIYIYSVSSDGAKSSRFGRCMGHSSFITHLDWSKDGNF
                     IMSNSGDYEILYWDVAGGCKQLKNRYESRDREWATYTCVLGFHVYGVWPDGSDGTDIN
                     SLCRSHNERVVAVADDFCKVHLFQYPCARAKAPSRMYGGHGSHVTSVRFTHDDSHLVS
                     LGGKDASIFQWRVLGAGGAGPAPATPSRTPSLSPASSLDV"
     exon            341..512
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            513..770
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            771..884
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            885..952
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1076
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1247
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1317
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1428
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1524
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1680
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1822
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..1974
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1975..2100
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            2101..2232
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..2300
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            2301..2389
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            2390..2487
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            2488..2575
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            2576..2674
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            2675..2805
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            2806..3266
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3245..3250
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /note="hexamer: AATAAA"
     polyA_site      3266
                     /gene="EML3"
                     /gene_synonym="ELP95; EMAP3; EMAP95"
                     /note="major polyA site"
ORIGIN      
        1 gcttcctggc ggctccgcgt ccggcgcctg tttgtgctgc ggcgctgtgg caccggacgg
       61 ccctgccacc ccacccccgg gagccgagag cggccccggg agagtccgag ggcccggggg
      121 ggtctggaga ctgcggcccc gggcccacag cgccccctcc aggccccttc cccgcgcccg
      181 acagcgcccc ctcggggtgg tggtacggcg cccttcgcgc gcgccccggg gtgcttcccc
      241 ttcccctctc cccggccgtg gcccccgcgg cttagacgcc tcctccgccg ccgccgctcg
      301 gagcaacccg ggggccggat ggacggggcc gcggggcccg gtgacggccc tgctcgggag
      361 gccctccagt ctctgagcca gcggcttcgg gtgcaggagc aggagatgga actggtaaag
      421 gcagccctgg cagaagccct tcgcctgctg cggctgcagg tgcccccttc ctccctgcag
      481 ggctctggca caccagctcc tccgggggac agtcttgcag cccccccagg actgccaccc
      541 acgtgcaccc cttccttggt gagccgaggc acccagacgg agacagaggt ggagctcaag
      601 tcatcccctg gaccccctgg cctgagcaat ggacccccag cccctcaggg ggccagcgaa
      661 gagcctagcg ggacccaatc tgaaggaggg ggcagcagca gcagtggtgc tggctcccct
      721 ggccccccgg ggatcctcag gcccttgcag cccccacagc gtgctgacac gccgcgaaga
      781 aattcttcct cctcctcatc cccctcagag cggcctcggc agaagctctc caggaaggca
      841 atctcctccg ccaacctgtt agtgcggtcc gggagcacag agagccgtgg gggaaaagac
      901 cccctctcca gccctggggg ccctggatct cggaggagca attacaattt ggaaggcatc
      961 tcagtgaaga tgttccttcg agggcgcccc attaccatgt acatcccgtc tggcatccgc
     1021 agccttgagg agctgccgag tggcccaccg ccagagaccc tcagccttga ctgggtttat
     1081 gggtacaggg gtcgtgactc ccgctctaat ctgtttgtgt tgcgctctgg ggaggtggtc
     1141 tactttatcg cctgtgtggt ggtgctgtac cggcctggag gaggcccagg gggtcctgga
     1201 ggtggcggcc agagacatta ccgggggcac acagactgcg ttcgatgcct tgctgttcac
     1261 cctgatggtg ttcgggtagc ctcgggacag acagctggag tggataagga tggaaagccc
     1321 ctgcagcctg tggttcacat ctgggactca gagacgctgt tgaaactgca ggagattgga
     1381 ctgggggcct tcgagcgggg tgttggggcc ctggcctttt cagctgcgga tcagggtgcc
     1441 tttctttgtg tggtggatga ttccaatgag cacatgctgt cggtgtggga ctgcagccgg
     1501 ggaatgaagc tggctgagat caagagtaca aatgactcag tcctggccgt tggcttcaac
     1561 cctcgtgaca gcagctgcat cgtcaccagt gggaaatctc acgtccactt ctggaattgg
     1621 agtggtggag taggggttcc tgggaatggg acccttaccc ggaaacaggg tgtctttggg
     1681 aaatacaaga aacccaagtt tatcccttgc tttgtgttcc ttccggatgg agacattctc
     1741 actggagact cagaggggaa cattctcacc tgggggcgga gcccttcaga ttccaagacc
     1801 ccaggcaggg gtggcgccaa agagacctat gggattgtgg cccaggctca cgctcatgaa
     1861 ggttctatct tcgccttgtg tctccggagg gacgggacag tgctgagtgg tggcgggcgg
     1921 gaccgccggc tggtacagtg ggggcccggg ttggtggccc tccaggaggc tgagattccc
     1981 gagcacttcg gggccgtgcg agccattgct gaagggcttg gctctgagct gctggtggga
     2041 accacgaaga atgcattgct gaggggagac ctggcccagg gcttctcccc tgtaatccag
     2101 ggccacactg atgagctctg ggggctctgc acacacccct cccagaaccg cttcctcacc
     2161 tgcggccacg accggcagct ctgcctgtgg gatggggaga gccatgcact ggcctggagc
     2221 atcgacctca aggagactgg tctctgtgct gacttccacc cgagtggggc agttgtggcc
     2281 gtaggactga acacggggag gtggttggtt ttggacacag agaccagaga gatcgtgtct
     2341 gatgtcattg atggcaatga gcagctctca gtggtccggt acagcccaga tgggttgtac
     2401 ctggccattg gttcccatga caacgtgatc tacatctata gtgtttccag tgatggtgcc
     2461 aaatccagcc gctttggccg ctgtatgggt cactccagct tcatcactca tcttgactgg
     2521 tccaaggatg ggaatttcat catgtccaat tctggggact atgagattct ttactgggac
     2581 gtggctggag gctgcaagca gctgaagaat cgctatgaga gccgagaccg ggaatgggct
     2641 acctacacct gtgtgctggg ctttcacgtc tacggcgtct ggccggacgg ctccgatggg
     2701 accgacatca actccctgtg ccgctcccac aacgagcgcg tggtggcggt ggccgacgac
     2761 ttctgcaaag tgcatctctt ccagtacccg tgcgctcgtg ccaaggcgcc gagccgcatg
     2821 tacgggggcc acggcagcca cgtgaccagc gtccgattca cgcacgacga ctcgcacctc
     2881 gtctcgctgg gcggcaagga cgccagcatc ttccagtggc gagtgctggg cgctgggggc
     2941 gcggggccgg cgcccgccac gccctctcga accccctccc tgtcccccgc ctcctccctc
     3001 gacgtttgat cgctgcctgg cgggaccgac tggcccggcg gcgtggcccc gccccgccct
     3061 gcccttccct ggcccaatcc cccacgacta ggggccgact ctttcctgga ctgacttcga
     3121 gacattcccg atcgcgcatt ttcctggagg gcgcgaacgg cgcccctgca cacactgttt
     3181 agacccgctg gctgagccgg gcagccccag cctaggcctt gactcccgct gcctgctgag
     3241 gggcaataaa ccagaaccaa agtcgc
//
