LOCUS       NM_012155               2234 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens EMAP like 2 (EML2), transcript variant 2, mRNA.
ACCESSION   NM_012155
VERSION     NM_012155.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Hotta,T., McAlear,T.S., Yue,Y., Higaki,T., Haynes,S.E.,
            Nesvizhskii,A.I., Sept,D., Verhey,K.J., Bechstedt,S. and Ohi,R.
  TITLE     EML2-S constitutes a new class of proteins that recognizes and
            regulates the dynamics of tyrosinated microtubules
  JOURNAL   Curr Biol 32 (18), 3898-3910 (2022)
   PUBMED   35963242
  REMARK    GeneRIF: EML2-S constitutes a new class of proteins that recognizes
            and regulates the dynamics of tyrosinated microtubules.
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Richards,M.W., O'Regan,L., Roth,D., Montgomery,J.M., Straube,A.,
            Fry,A.M. and Bayliss,R.
  TITLE     Microtubule association of EML proteins and the EML4-ALK variant 3
            oncoprotein require an N-terminal trimerization domain
  JOURNAL   Biochem J 467 (3), 529-536 (2015)
   PUBMED   25740311
  REMARK    GeneRIF: We have determined crystal structures of the coiled-coils
            from EML2 and EML4, which describe the structural basis of both EML
            self-association and oncogenic EML4-ALK activation.
REFERENCE   3  (bases 1 to 2234)
  AUTHORS   Richards,M.W., Law,E.W., Rennalls,L.P., Busacca,S., O'Regan,L.,
            Fry,A.M., Fennell,D.A. and Bayliss,R.
  TITLE     Crystal structure of EML1 reveals the basis for Hsp90 dependence of
            oncogenic EML4-ALK by disruption of an atypical beta-propeller
            domain
  JOURNAL   Proc Natl Acad Sci U S A 111 (14), 5195-5200 (2014)
   PUBMED   24706829
REFERENCE   4  (bases 1 to 2234)
  AUTHORS   Green,L.A., Yi,R., Petrusca,D., Wang,T., Elghouche,A., Gupta,S.K.,
            Petrache,I. and Clauss,M.
  TITLE     HIV envelope protein gp120-induced apoptosis in lung microvascular
            endothelial cells by concerted upregulation of EMAP II and its
            receptor, CXCR3
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 306 (4), L372-L382 (2014)
   PUBMED   24318111
  REMARK    GeneRIF: Both EMAP II and CXCR3 were essentially required for
            gp120-induced lung microvascular endothelial cell apoptosis.
REFERENCE   5  (bases 1 to 2234)
  AUTHORS   Fox,C.S., Liu,Y., White,C.C., Feitosa,M., Smith,A.V.,
            Heard-Costa,N., Lohman,K., Johnson,A.D., Foster,M.C.,
            Greenawalt,D.M., Griffin,P., Ding,J., Newman,A.B., Tylavsky,F.,
            Miljkovic,I., Kritchevsky,S.B., Launer,L., Garcia,M.,
            Eiriksdottir,G., Carr,J.J., Gudnason,V., Harris,T.B., Cupples,L.A.
            and Borecki,I.B.
  CONSRTM   GIANT Consortium; MAGIC Consortium; GLGC Consortium
  TITLE     Genome-wide association for abdominal subcutaneous and visceral
            adipose reveals a novel locus for visceral fat in women
  JOURNAL   PLoS Genet 8 (5), e1002695 (2012)
   PUBMED   22589738
REFERENCE   6  (bases 1 to 2234)
  AUTHORS   Eichenmuller,B., Everley,P., Palange,J., Lepley,D. and
            Suprenant,K.A.
  TITLE     The human EMAP-like protein-70 (ELP70) is a microtubule
            destabilizer that localizes to the mitotic apparatus
  JOURNAL   J Biol Chem 277 (2), 1301-1309 (2002)
   PUBMED   11694528
REFERENCE   7  (bases 1 to 2234)
  AUTHORS   Lepley,D.M., Palange,J.M. and Suprenant,K.A.
  TITLE     Sequence and expression patterns of a human EMAP-related protein-2
            (HuEMAP-2)
  JOURNAL   Gene 237 (2), 343-349 (1999)
   PUBMED   10521658
REFERENCE   8  (bases 1 to 2234)
  AUTHORS   Adams,M.D., Soares,M.B., Kerlavage,A.R., Fields,C. and Venter,J.C.
  TITLE     Rapid cDNA sequencing (expressed sequence tags) from a
            directionally cloned human infant brain cDNA library
  JOURNAL   Nat Genet 4 (4), 373-380 (1993)
   PUBMED   8401585
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF103939.1.
            
            On Jun 2, 2019 this sequence version replaced NM_012155.3.
            
            Transcript Variant: This variant (2) differs in the 5' UTR and 5'
            coding region which results in the use of a different start codon,
            compared to variant 1, and an isoform (2) with a shorter and
            distinct N-terminus, compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF103939.1, AL096717.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000245925.8/ ENSP00000245925.3
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2234              AF103939.1         15-2248
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.32"
     gene            1..2234
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /note="EMAP like 2"
                     /db_xref="GeneID:24139"
                     /db_xref="HGNC:HGNC:18035"
                     /db_xref="MIM:617494"
     exon            1..41
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..1971
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     microtubule-associated protein like echinoderm EMAP;
                     echinoderm MT-associated protein (EMAP)-like protein 70;
                     echinoderm microtubule associated protein like 2"
                     /codon_start=1
                     /product="echinoderm microtubule-associated protein-like 2
                     isoform 2"
                     /protein_id="NP_036287.1"
                     /db_xref="CCDS:CCDS12670.1"
                     /db_xref="GeneID:24139"
                     /db_xref="HGNC:HGNC:18035"
                     /db_xref="MIM:617494"
                     /translation="MSSFGAGKTKEVIFSVEDGSVKMFLRGRPVPMMIPDELAPTYSL
                     DTRSELPSCRLKLEWVYGYRGRDCRANLYLLPTGEIVYFVASVAVLYSVEEQRQRHYL
                     GHNDDIKCLAIHPDMVTIATGQVAGTTKEGKPLPPHVRIWDSVSLSTLHVLGLGVFDR
                     AVCCVGFSKSNGGNLLCAVDESNDHMLSVWDWAKETKVVDVKCSNEAVLVATFHPTDP
                     TVLITCGKSHIYFWTLEGGSLSKRQGLFEKHEKPKYVLCVTFLEGGDVVTGDSGGNLY
                     VWGKGGNRITQAVLGAHDGGVFGLCALRDGTLVSGGGRDRRVVLWGSDYSKLQEVEVP
                     EDFGPVRTVAEGHGDTLYVGTTRNSILQGSVHTGFSLLVQGHVEELWGLATHPSRAQF
                     VTCGQDKLVHLWSSDSHQPLWSRIIEDPARSAGFHPSGSVLAVGTVTGRWLLLDTETH
                     DLVAIHTDGNEQISVVSFSPDGAYLAVGSHDNLVYVYTVDQGGRKVSRLGKCSGHSSF
                     ITHLDWAQDSSCFVTNSGDYEILYWDPATCKQITSADAVRNMEWATATCVLGFGVFGI
                     WSEGADGTDINAVARSHDGKLLASADDFGKVHLFSYPCCQPRALSHKYGGHSSHVTNV
                     AFLWDDSMALTTGGKDTSVLQWRVV"
     exon            42..70
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            71..200
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            201..350
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            351..420
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            421..531
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            532..627
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            628..762
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            763..862
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            863..1017
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1143
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1275
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1343
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1432
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1530
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1618
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1619..1714
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1715..1845
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..2234
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2215..2220
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /note="hexamer: AATAAA"
     polyA_site      2234
                     /gene="EML2"
                     /gene_synonym="ELP70; EMAP-2; EMAP2"
                     /note="major polyA site"
ORIGIN      
        1 gaagctccgc cacccgccgc catgagtagc tttggagctg gcaaaaccaa agaagttatc
       61 ttcagtgtgg aggatggctc cgtgaaaatg ttcctgaggg gccgccctgt gcccatgatg
      121 atcccagacg agctggcacc cacctacagc ctggacacac gctcggagct gccttcttgc
      181 cggctcaagc tggagtgggt ctatggctac cgtggccgag actgccgggc caacctttat
      241 ttgctgccca ccggggagat agtgtacttt gtggcctccg tagccgtgct atacagcgtg
      301 gaggagcaga ggcagcgaca ctacctggga cacaacgatg acatcaaatg cttggccatc
      361 cacccagata tggtcaccat cgccacggga caggtggcgg gaaccactaa ggaagggaag
      421 ccgctgccgc cccacgtgcg catctgggac tcagtttccc tctccacctt acacgtgctg
      481 ggcttggggg tgtttgacag agccgtgtgc tgtgtgggct tctccaaatc taatggaggc
      541 aacctgctgt gtgcagtgga tgaatccaat gatcacatgc tctcggtgtg ggactgggcc
      601 aaggagacca aggtggtgga tgtcaagtgc tccaatgagg ctgtattggt ggccaccttc
      661 caccccacgg accccactgt gcttatcacc tgcgggaaat ctcacatcta cttctggacc
      721 ttggaggggg gcagcttgag caagcggcaa ggcctctttg agaaacatga gaaaccgaag
      781 tatgtgctgt gtgtgacctt tttggaaggt ggcgacgtgg tcacggggga ctctgggggg
      841 aacctctatg tttggggcaa aggtgggaac cgtatcacac aggcggtgct gggcgcccac
      901 gacggcggcg tgtttgggct ctgcgccctg cgggacggga cgctggtgtc tggagggggc
      961 cgtgatcggc gggtggtcct ctggggttct gactacagca agctgcagga agtggaggtc
     1021 cctgaggact ttggccctgt gcgcaccgtg gcagagggcc acggagacac actgtacgtg
     1081 gggaccaccc gcaattccat cctgcagggc tccgtgcaca caggcttctc actgctggtc
     1141 cagggccatg tggaagagct gtggggcctg gccacacacc ccagtcgggc ccagtttgtg
     1201 acctgcgggc aggataagct ggtgcatcta tggagctcag attcccacca gcccctgtgg
     1261 agcaggatca tcgaggaccc tgcccgctca gccggcttcc accccagtgg ctctgtcctg
     1321 gctgtgggta cagtgactgg cagatggctg ctgctggaca cggagaccca tgacctggtg
     1381 gctatccaca cagacggcaa tgaacagatc tcagtggtca gcttctcccc agacggggcg
     1441 tacctggccg tgggctccca cgacaacttg gtgtacgtgt acacggtgga ccagggcggc
     1501 cgcaaggtca gccgcctggg caagtgctcg ggccattcca gttttatcac ccacctggat
     1561 tgggcccagg acagcagctg ctttgtcacc aactccgggg actatgagat tctgtactgg
     1621 gacccggcta cctgtaagca gatcaccagt gcggatgctg tgaggaacat ggaatgggcc
     1681 acagctactt gtgtcctagg gtttggggtg tttgggatct ggtctgaggg ggcggacggc
     1741 actgatatca acgctgtggc ccgctctcat gatgggaagt tgctggcttc agctgatgac
     1801 tttggcaaag ttcacctgtt tagctacccc tgctgtcagc ctcgagccct cagccacaag
     1861 tacggtggac acagcagcca tgtgacaaat gtggccttct tgtgggatga cagcatggcc
     1921 ctgaccacag ggggcaagga caccagtgtg ctacagtggc gggtggtctg atgcggccag
     1981 ggaagagtca ggtgtcaggg caggaattct attttcggga gatgtctatt gccgagtaga
     2041 gtaatatata cccagagtat gtctatagca gagggggtta tgggggcggg agggtagact
     2101 gacatacaga agtctctatt tatccgggtg ggaagaggga gtcacatcgc tttggggatc
     2161 cattggtgtt tggtttgggg tgttttttaa gttttttctt ttatatcatc cagaaataaa
     2221 gacacgtaca ctaa
//
