LOCUS       NM_004434               4460 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens EMAP like 1 (EML1), transcript variant 2, mRNA.
ACCESSION   NM_004434
VERSION     NM_004434.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Jabali,A., Hoffrichter,A., Uzquiano,A., Marsoner,F., Wilkens,R.,
            Siekmann,M., Bohl,B., Rossetti,A.C., Horschitz,S., Koch,P.,
            Francis,F. and Ladewig,J.
  TITLE     Human cerebral organoids reveal progenitor pathology in EML1-linked
            cortical malformation
  JOURNAL   EMBO Rep 23 (5), e54027 (2022)
   PUBMED   35289477
  REMARK    GeneRIF: Human cerebral organoids reveal progenitor pathology in
            EML1-linked cortical malformation.
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Markus,F., Kannengiesser,A., Nader,P., Atigbire,P., Scholten,A.,
            Vossing,C., Bultmann,E., Korenke,G.C., Owczarek-Lipska,M. and
            Neidhardt,J.
  TITLE     A novel missense variant in the EML1 gene associated with bilateral
            ribbon-like subcortical heterotopia leads to ciliary defects
  JOURNAL   J Hum Genet 66 (12), 1159-1167 (2021)
   PUBMED   34211111
  REMARK    GeneRIF: A novel missense variant in the EML1 gene associated with
            bilateral ribbon-like subcortical heterotopia leads to ciliary
            defects.
REFERENCE   3  (bases 1 to 4460)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 4460)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 4460)
  AUTHORS   Oegema,R., McGillivray,G., Leventer,R., Le Moing,A.G.,
            Bahi-Buisson,N., Barnicoat,A., Mandelstam,S., Francis,D.,
            Francis,F., Mancini,G.M.S., Savelberg,S., van Haaften,G., Mankad,K.
            and Lequin,M.H.
  TITLE     EML1-associated brain overgrowth syndrome with ribbon-like
            heterotopia
  JOURNAL   Am J Med Genet C Semin Med Genet 181 (4), 627-637 (2019)
   PUBMED   31710781
  REMARK    GeneRIF: biallelic EML1 disease-causing variants cause a highly
            specific pattern of congenital brain malformations, severe
            developmental delay, seizures and visual impairment
            Review article
REFERENCE   6  (bases 1 to 4460)
  AUTHORS   Ly,C.D., Roche,K.W., Lee,H.K. and Wenthold,R.J.
  TITLE     Identification of rat EMAP, a delta-glutamate receptor binding
            protein
  JOURNAL   Biochem Biophys Res Commun 291 (1), 85-90 (2002)
   PUBMED   11829466
REFERENCE   7  (bases 1 to 4460)
  AUTHORS   Lepley,D.M., Palange,J.M. and Suprenant,K.A.
  TITLE     Sequence and expression patterns of a human EMAP-related protein-2
            (HuEMAP-2)
  JOURNAL   Gene 237 (2), 343-349 (1999)
   PUBMED   10521658
REFERENCE   8  (bases 1 to 4460)
  AUTHORS   Schaefer,G.B., Bodensteiner,J.B., Thompson,J.N. Jr.,
            Kimberling,W.J. and Craft,J.M.
  TITLE     Volumetric neuroimaging in Usher syndrome: evidence of global
            involvement
  JOURNAL   Am J Med Genet 79 (1), 1-4 (1998)
   PUBMED   9738858
REFERENCE   9  (bases 1 to 4460)
  AUTHORS   Eudy,J.D., Ma-Edmonds,M., Yao,S.F., Talmadge,C.B., Kelley,P.M.,
            Weston,M.D., Kimberling,W.J. and Sumegi,J.
  TITLE     Isolation of a novel human homologue of the gene coding for
            echinoderm microtubule-associated protein (EMAP) from the Usher
            syndrome type 1a locus at 14q32
  JOURNAL   Genomics 43 (1), 104-106 (1997)
   PUBMED   9226380
REFERENCE   10 (bases 1 to 4460)
  AUTHORS   Kaplan,J., Gerber,S., Bonneau,D., Rozet,J.M., Delrieu,O.,
            Briard,M.L., Dollfus,H., Ghazi,I., Dufier,J.L., Frezal,J. et al.
  TITLE     A gene for Usher syndrome type I (USH1A) maps to chromosome 14q
  JOURNAL   Genomics 14 (4), 979-987 (1992)
   PUBMED   1478676
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX420178.2, BC033043.1,
            BX442035.2, BC032541.2 and AF035276.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_004434.2.
            
            Summary: Human echinoderm microtubule-associated protein-like is a
            strong candidate for the Usher syndrome type 1A gene. Usher
            syndromes (USHs) are a group of genetic disorders consisting of
            congenital deafness, retinitis pigmentosa, and vestibular
            dysfunction of variable onset and severity depending on the genetic
            type. The disease process in USHs involves the entire brain and is
            not limited to the posterior fossa or auditory and visual systems.
            The USHs are catagorized as type I (USH1A, USH1B, USH1C, USH1D,
            USH1E and USH1F), type II (USH2A and USH2B) and type III (USH3).
            The type I is the most severe form. Gene loci responsible for these
            three types are all mapped. Two transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (b) has the same
            N- and C-termini but is shorter compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.20526.1,
                                           SRR1803616.32325.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262233.11/ ENSP00000262233.7
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-434               BX420178.2         17-450
            435-1717            BC033043.1         440-1722
            1718-2238           BX442035.2         279-799
            2239-3702           BC032541.2         2197-3660
            3703-4460           AF035276.1         372-1129
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.2"
     gene            1..4460
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /note="EMAP like 1"
                     /db_xref="GeneID:2009"
                     /db_xref="HGNC:HGNC:3330"
                     /db_xref="MIM:602033"
     exon            1..131
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..2512
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /note="isoform b is encoded by transcript variant 2;
                     echinoderm microtubule associated protein like 1"
                     /codon_start=1
                     /product="echinoderm microtubule-associated protein-like 1
                     isoform b"
                     /protein_id="NP_004425.2"
                     /db_xref="CCDS:CCDS32155.1"
                     /db_xref="GeneID:2009"
                     /db_xref="HGNC:HGNC:3330"
                     /db_xref="MIM:602033"
                     /translation="MEDGFSSYSSLYDTSSLLQFCNDDSASAASSMEVTDRIASLEQR
                     VQMQEDDIQLLKSALADVVRRLNITEEQQAVLNRKGPTKARPLMQTLPLRTTVNNGTV
                     LPKKPTGSLPSPSGVRKETAVPATKSNIKRTSSSERVSPGGRRESNGDSRGNRNRTGS
                     TSSSSSGKKNSESKPKEPVFSAEEGYVKMFLRGRPVTMYMPKDQVDSYSLEAKVELPT
                     KRLKLEWVYGYRGRDCRNNLYLLPTGETVYFIASVVVLYNVEEQLQRHYAGHNDDVKC
                     LAVHPDRITIATGQVAGTSKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAFS
                     KSNGGTNLCAVDDSNDHVLSVWDWQKEEKLADVKCSNEAVFAADFHPTDTNIIVTCGK
                     SHLYFWTLEGSSLNKKQGLFEKQEKPKFVLCVTFSENGDTITGDSSGNILVWGKGTNR
                     ISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRKLISWSGNYQKLRKTEIPEQFGPIRT
                     VAEGKGDVILIGTTRNFVLQGTLSGDFTPITQGHTDELWGLAIHASKSQFLTCGHDKH
                     ATLWDAVGHRPVWDKIIEDPAQSSGFHPSGSVVAVGTLTGRWFVFDTETKDLVTVHTD
                     GNEQLSVMRYSPDGNFLAIGSHDNCIYIYGVSDNGRKYTRVGKCSGHSSFITHLDWSV
                     NSQFLVSNSGDYEILYWVPSACKQVVSVETTRDIEWATYTCTLGFHVFGVWPEGSDGT
                     DINAVCRAHEKKLLSTGDDFGKVHLFSYPCSQFRAPSHIYGGHSSHVTNVDFLCEDSH
                     LISTGGKDTSIMQWRVI"
     exon            132..314
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            315..447
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            448..582
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            583..611
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            612..741
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            742..891
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            892..961
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1072
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1168
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1303
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1403
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1558
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1559..1684
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1816
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1817..1884
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..1973
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1974..2071
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..2159
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2255
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            2256..2386
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            2387..4460
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3681..3686
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /note="hexamer: AATAAA"
     polyA_site      3702
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /note="major polyA site"
     regulatory      3919..3924
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /note="hexamer: AATAAA"
     polyA_site      3938
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /note="major polyA site"
     regulatory      4439..4444
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /note="hexamer: AATGAA"
     polyA_site      4460
                     /gene="EML1"
                     /gene_synonym="BH; ELP79; EMAP; EMAP-1; EMAPL"
                     /note="major polyA site"
ORIGIN      
        1 agtgtgtggt gagcggcggc ggcgcggccg ggccggggag cgggcgcggc ccggcggcct
       61 cagcatggag gacggcttct ccagctacag cagcctgtac gacacgtcct cgctgctcca
      121 gttctgcaac gatgacagcg cttctgctgc aagtagcatg gaggtgacag accgcattgc
      181 ttcactggag cagagagtcc agatgcaaga agacgacatc cagctgctca aatcagctct
      241 agctgatgtg gttcggcggc tgaacattac tgaggaacag caggccgtgc ttaacaggaa
      301 aggacctacc aaagcaagac cactgatgca gaccctgcct ttaagaacca cggtcaacaa
      361 tggcactgtg ttaccaaaga aacctactgg ctctctacca tccccctccg gggtcaggaa
      421 agaaactgct gtgccagcaa ccaaaagtaa catcaagagg accagctctt ctgaacgagt
      481 gtctcctggg ggtcgaaggg aaagcaatgg ggattccaga ggaaaccgga atcgcacagg
      541 ctccaccagc agctcttcca gtggcaaaaa gaacagtgaa agcaaaccca aggagcctgt
      601 attcagtgca gaagaaggct atgtaaaaat gtttcttcgt ggacgccctg ttaccatgta
      661 catgcccaaa gatcaagtgg attcttacag cttggaagca aaagtagaac ttccaaccaa
      721 gagactcaag ctggaatggg tctatgggta caggggtcga gactgccgta acaacctgta
      781 cttgcttccg acgggagaga ccgtctactt catcgcatcc gtggtggtgt tatacaacgt
      841 ggaggagcaa ctgcagaggc attacgctgg ccacaacgat gacgtgaagt gcctagcagt
      901 tcatcctgat cggatcacga tagcaacagg acaagttgcg ggcacatcga aggatggaaa
      961 acaattgccc ccacatgtgc gcatctggga ttctgtgaca ttgaatactc tccacgtcat
     1021 tggaataggt ttttttgacc gagcagtcac ctgtattgca ttctcaaaat ctaatggagg
     1081 aaccaatctc tgtgctgtgg atgactccaa cgaccatgtg ctctctgtat gggactggca
     1141 gaaagaagaa aaactagcag atgtgaagtg ctctaatgaa gctgtgtttg ctgcggattt
     1201 ccaccccacg gacaccaaca tcatagttac ttgtggaaaa tcacatctct acttttggac
     1261 actagaagga agctccctta ataagaagca aggattattc gagaaacaag aaaagccaaa
     1321 gtttgtcctc tgtgtgactt tctctgaaaa cggtgacacc attactggag attcaagtgg
     1381 caacatctta gtatggggaa aaggtacaaa tcgaataagc tatgcagttc agggggccca
     1441 tgagggtggc atttttgcac tttgtatgtt aagagatggc acactggtgt cgggaggtgg
     1501 gaaagaccga aagctcattt cttggagcgg aaactatcaa aaacttcgta aaacggagat
     1561 tccagaacag tttggtccaa tacggacagt ggccgagggg aaaggcgatg tgatcttgat
     1621 tggcacaact cgaaactttg tcctgcaggg cactctgtca ggggacttca cacccattac
     1681 tcagggtcac actgatgagc tctggggact ggccatccat gcctcaaaat ctcagttctt
     1741 gacctgtggg catgacaagc atgccactct ctgggacgct gtgggtcacc gtcccgtctg
     1801 ggacaaaata atagaggatc cagctcagtc ttctggtttt catccttcag ggtctgtggt
     1861 tgcagtcgga acactcactg ggaggtggtt tgtgtttgac acagaaacaa aagacttggt
     1921 caccgttcac acagatggaa acgaacagct ctctgtaatg cgatactcac cagatgggaa
     1981 tttcttagcc ataggctcac atgacaactg catctatata tatggcgtta gtgacaacgg
     2041 gaggaagtac acgcgagtgg gcaagtgctc gggtcattcc agcttcatta ctcacctgga
     2101 ctggtctgta aactcacagt tcctcgtgtc aaattccgga gactacgaaa tcctctactg
     2161 ggttccctct gcctgtaagc aagtcgtaag tgtggaaact acaagagaca ttgaatgggc
     2221 tacctatacc tgcactttgg gattccatgt ttttggagtg tggccagaag gctcggacgg
     2281 aaccgacatc aatgccgtct gtcgggccca tgagaagaaa ctcctgtcaa caggcgacga
     2341 ctttggcaaa gtgcacctct tctcataccc ctgctcgcag ttcagggctc caagccacat
     2401 ctacggcggg cacagcagcc atgtcaccaa tgtcgatttc ctctgtgaag acagccacct
     2461 catctccacg ggcgggaaag acacaagcat catgcagtgg cgcgtcattt agtacccacc
     2521 gagagctgtg gggagcagca tgggcaagga agacacagac tcgcattacc cttggtcact
     2581 gtgatttctg ttttgtttaa aaaattctta caaacctcag gaaaactgtg ccctccgccg
     2641 gctaccttag cttagcgtgt cagcgggcgc cacagcggat cagcggttcc gtgttcactt
     2701 ttgttgtaca atatatgaca cagtgcacat tgaataccaa caaggttgca acgtttacat
     2761 tatagccaca tcaacagaag taactggtat attcttagta acttttctat gaactcttca
     2821 aaaatggtca cagaatgcct tttaaaacat tgtatataat cttcactgtt tcaccatcta
     2881 gcttgctaag tcaaatattt atgatgataa tgaggtactg aaccacgatg gctgttgagg
     2941 aattggtcct aaaaggacag atcacttcag aagagtgaat aactgatttg cacagctgaa
     3001 tcaggagaca caaagatgag actgtgtttg gttacatttt ccaaagtttc attgcattct
     3061 cccttgggga ggctgtgaga gagggcttgt atccctcttg tgctaagcag actctactcc
     3121 taactgactt caatatttca gcagggtaca caggcgtttc caagtttcag tgacaccgtc
     3181 ctgcctaacc agatgcggtc agcctcttca cacccacctg gcttgcatcc cccatccctt
     3241 gttcacacgc cctgattcac ggtgagacat tttgccacct tcttgtgtat attacttggc
     3301 atgagatgat attgtacttg tataggattc tagcaattca taataaatat gtaagactag
     3361 gctttactgt cttatgctta tggacattgt atatttgtat tttatgacca agtagaccaa
     3421 gtcagaaaga tctctctcga gcgtaccata aacctgcaga gagaagtctc gaaaggctcc
     3481 accaggtacc aagggcagct gcttttcctg tcttttgtgc atgggcgacc cattacagta
     3541 tgagataaga ttgagttctg atgcgttaaa cggaggtggc agaaatttgt caagaaggcc
     3601 ttatccattt cgattgtgtg acagattgaa atttattgtt tacattgggg aatgtatctc
     3661 aaatttttaa atagaagagt aataaacaga ctttaaagca aatattaaga tttttactca
     3721 ttcaaggcaa gtaaatgaat ggaattatct gagctctatg gcactggttg tttagagtga
     3781 ctgatgaagt gcaactttca aaaacatttt tgatgacatc accagcctac tgcagaagtg
     3841 cagggcacca gtaaacacca tgtattattg aagatgaatc tgtttgtatg tatccttgtc
     3901 aaatatattc tataatgaaa taaaatctga aaagtggatt tcttattgac ctatattcat
     3961 gaaagcatat aaattaaaat atttaaaatt agatatgatt cacactatat tctgtttcat
     4021 atgcagattt tattctcacc tgggccattt gcagatgaga ctgtagtttg cagatgagac
     4081 tgtagtttgc agatggcgtg gaagcattca tcaggggaga taaccataaa ggatttggcc
     4141 taattaccat actcaattgt cagtttacgt ggttttgtga atactggcaa aagcaattgt
     4201 ttttaaatta acaatggaga gaatgataag atgagggaag gaaaaggcat tcattattga
     4261 cttacatgtc agtaaggtct gcttttattt ctatgtactc ctgtttgcca agctcaataa
     4321 tggacaaagg atacaaacac acacacatct actattttag ataaatgtac tgttatatat
     4381 atatgtaaac tactattgct ctctttataa tgattaatca ctttattatg aatgaatgaa
     4441 tgaatttgat ggatttaaaa
//
