LOCUS       NM_001257370            7022 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens essential meiotic structure-specific endonuclease
            subunit 2 (EME2), mRNA.
ACCESSION   NM_001257370
VERSION     NM_001257370.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Hua,Z., Fang,Q., Zhang,D., Luo,Z., Yuan,C. and Lin,Z.
  TITLE     Crystal structure of the human MUS81-EME2 complex
  JOURNAL   Structure 30 (5), 743-752 (2022)
   PUBMED   35290797
  REMARK    GeneRIF: Crystal structure of the human MUS81-EME2 complex.
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Techer,H., Koundrioukoff,S., Carignon,S., Wilhelm,T., Millot,G.A.,
            Lopez,B.S., Brison,O. and Debatisse,M.
  TITLE     Signaling from Mus81-Eme2-Dependent DNA Damage Elicited by Chk1
            Deficiency Modulates Replication Fork Speed and Origin Usage
  JOURNAL   Cell Rep 14 (5), 1114-1127 (2016)
   PUBMED   26804904
  REMARK    GeneRIF: Avoiding damage formation through invalidation of
            Mus81-Eme2 and Mre11, or preventing damage signaling by turning off
            the ATM pathway, suppresses the replication phenotypes of
            Chk1-deficient cells.
REFERENCE   3  (bases 1 to 7022)
  AUTHORS   Pepe,A. and West,S.C.
  TITLE     MUS81-EME2 promotes replication fork restart
  JOURNAL   Cell Rep 7 (4), 1048-1055 (2014)
   PUBMED   24813886
  REMARK    GeneRIF: Results define distinct and temporal roles for MUS81-EME1
            and MUS81-EME2 in the maintenance of genome stability.
REFERENCE   4  (bases 1 to 7022)
  AUTHORS   Amangyeld,T., Shin,Y.K., Lee,M., Kwon,B. and Seo,Y.S.
  TITLE     Human MUS81-EME2 can cleave a variety of DNA structures including
            intact Holliday junction and nicked duplex
  JOURNAL   Nucleic Acids Res 42 (9), 5846-5862 (2014)
   PUBMED   24692662
  REMARK    GeneRIF: The presence of a 5' phosphate terminus at nicks and gaps
            rendered DNA significantly less susceptible to the cleavage by
            MUS81-EME2 than its absence.
REFERENCE   5  (bases 1 to 7022)
  AUTHORS   Pepe,A. and West,S.C.
  TITLE     Substrate specificity of the MUS81-EME2 structure selective
            endonuclease
  JOURNAL   Nucleic Acids Res 42 (6), 3833-3845 (2014)
   PUBMED   24371268
  REMARK    GeneRIF: The results indicate that MUS81-EME2 is a more active
            endonuclease than MUS81-EME1 and exhibits broader substrate
            specificity.
REFERENCE   6  (bases 1 to 7022)
  AUTHORS   Ciccia,A., Ling,C., Coulthard,R., Yan,Z., Xue,Y., Meetei,A.R.,
            Laghmani el,H., Joenje,H., McDonald,N., de Winter,J.P., Wang,W. and
            West,S.C.
  TITLE     Identification of FAAP24, a Fanconi anemia core complex protein
            that interacts with FANCM
  JOURNAL   Mol Cell 25 (3), 331-343 (2007)
   PUBMED   17289582
REFERENCE   7  (bases 1 to 7022)
  AUTHORS   Ciccia,A., Constantinou,A. and West,S.C.
  TITLE     Identification and characterization of the human mus81-eme1
            endonuclease
  JOURNAL   J Biol Chem 278 (27), 25172-25178 (2003)
   PUBMED   12721304
REFERENCE   8  (bases 1 to 7022)
  AUTHORS   Daniels,R.J., Peden,J.F., Lloyd,C., Horsley,S.W., Clark,K.,
            Tufarelli,C., Kearney,L., Buckle,V.J., Doggett,N.A., Flint,J. and
            Higgs,D.R.
  TITLE     Sequence, structure and pathology of the fully annotated terminal 2
            Mb of the short arm of human chromosome 16
  JOURNAL   Hum Mol Genet 10 (4), 339-352 (2001)
   PUBMED   11157797
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC012180.6.
            
            On Sep 25, 2020 this sequence version replaced NM_001257370.1.
            
            Summary: EME2 forms a heterodimer with MUS81 (MIM 606591) that
            functions as an XPF (MIM 278760)-type flap/fork endonuclease in DNA
            repair (Ciccia et al., 2007 [PubMed 17289582]).[supplied by OMIM,
            Mar 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.4017129.1,
                                           SRR14038191.760246.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2145893
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000568449.7/ ENSP00000457353.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-665               AC012180.6         9821-10485
            666-802             AC012180.6         10716-10852
            803-895             AC012180.6         11271-11363
            896-987             AC012180.6         12052-12143
            988-1081            AC012180.6         12326-12419
            1082-1197           AC012180.6         12580-12695
            1198-1387           AC012180.6         12808-12997
            1388-7022           AC012180.6         13079-18713
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..7022
                     /gene="EME2"
                     /gene_synonym="gs125; SLX2B"
                     /note="essential meiotic structure-specific endonuclease
                     subunit 2"
                     /db_xref="GeneID:197342"
                     /db_xref="HGNC:HGNC:27289"
                     /db_xref="MIM:610886"
     exon            1..665
                     /gene="EME2"
                     /gene_synonym="gs125; SLX2B"
                     /inference="alignment:Splign:2.1.0"
     CDS             419..1558
                     /gene="EME2"
                     /gene_synonym="gs125; SLX2B"
                     /note="homolog of yeast EME1 endonuclease 2; SLX2
                     structure-specific endonuclease subunit homolog B;
                     essential meiotic endonuclease 1 homolog 2"
                     /codon_start=1
                     /product="probable crossover junction endonuclease EME2"
                     /protein_id="NP_001244299.1"
                     /db_xref="CCDS:CCDS58404.1"
                     /db_xref="GeneID:197342"
                     /db_xref="HGNC:HGNC:27289"
                     /db_xref="MIM:610886"
                     /translation="MARVGPGRAGVSCQGRGRGRGGSGQRRPPTWEISDSDAEDSAGS
                     EAAARARDPAGERRAAAEALRLLRPEQVLKRLAVCVDTAILEDAGADVLMEALEALGC
                     ECRIEPQRPARSLRWTRASPDPCPRSLPPEVWAAGEQELLLLLEPEEFLQGVATLTQI
                     SGPTHWVPWISPETTARPHLAVIGLDAYLWSRQHVSRGTQQPESPKVAGAEVAVSWPE
                     VEEALVLLQLWANLDVLLVASWQELSRHVCAVTKALAQYPLKQYRESQAFSFCTAGRW
                     AAGEPVARDGAGLQAAWRRQIRQFSRVSPAVADAVVTAFPSPRLLQQALEACSTERER
                     MGLLADLPVPPSEGGRPRRVGPDLSRRICLFLTTANPDLLLDLGS"
     exon            666..802
                     /gene="EME2"
                     /gene_synonym="gs125; SLX2B"
                     /inference="alignment:Splign:2.1.0"
     exon            803..895
                     /gene="EME2"
                     /gene_synonym="gs125; SLX2B"
                     /inference="alignment:Splign:2.1.0"
     exon            896..987
                     /gene="EME2"
                     /gene_synonym="gs125; SLX2B"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1081
                     /gene="EME2"
                     /gene_synonym="gs125; SLX2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1197
                     /gene="EME2"
                     /gene_synonym="gs125; SLX2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1387
                     /gene="EME2"
                     /gene_synonym="gs125; SLX2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..7022
                     /gene="EME2"
                     /gene_synonym="gs125; SLX2B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6498..6503
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EME2"
                     /gene_synonym="gs125; SLX2B"
                     /note="hexamer: ATTAAA"
     polyA_site      6567
                     /gene="EME2"
                     /gene_synonym="gs125; SLX2B"
     regulatory      6991..6996
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EME2"
                     /gene_synonym="gs125; SLX2B"
                     /note="hexamer: AATAAA"
     polyA_site      7022
                     /gene="EME2"
                     /gene_synonym="gs125; SLX2B"
                     /note="major polyA site"
ORIGIN      
        1 agtcgggccg cacccgcgtg aggcgccagt agcacggctc gtcgtgctgc cacagccagg
       61 acttgcgcgt gaccaggcgg cccaggccga agagcgggag gcggccgagc agctgcaaga
      121 ggcggctctc gcggcgcacg tcggcccagg cccggaccgg cagccggcgt ccagagaacg
      181 gccgcgtcaa ggtctcgtag tccaccgcgt agagctggga gtcgcgcggc ctgttcagtt
      241 gctcccgcag ggcgcgcacg cggcgggcca gctccgcgat cagccgcgga cgcaccttct
      301 tccgcgccat ggcgggtccg cgtcctcagc ggtccggccg gaagtcaccg gaagaggccg
      361 gtgtcccagg ctaaagtgtt cggtcgcggc cggaagcgag gaagaggtcg gtccggccat
      421 ggcgcgggtt ggacccggga gggcgggggt ctcttgccag ggccggggcc ggggacgggg
      481 cgggagcggt cagcggcgac ctccaacctg ggagatctca gactccgacg ctgaggactc
      541 cgccggctcg gaggccgccg cgagagcccg ggacccagcg ggtgagcgca gggcggctgc
      601 cgaggcgttg cggctgctgc ggccggagca ggtcctgaag cgcctcgcgg tgtgcgtgga
      661 cacagccatc ctggaagacg ccggtgccga cgtcctgatg gaggccctgg aggccctggg
      721 ctgcgagtgc cgcatcgagc cccagcgccc ggcccgcagc ctgcggtgga cccgagcgag
      781 tcccgacccc tgcccccgca gcctgcctcc tgaagtgtgg gctgcaggtg aacaggaatt
      841 gctgctgctg ctggagcccg aggagtttct gcagggcgtc gccacactga cccagatctc
      901 tggcccaacc cactgggtgc cctggatctc ccccgagacc accgcccggc cccacctggc
      961 tgtcatcggg ctggatgcct acctgtggtc tcgccagcac gtttcccggg ggacacagca
     1021 gccagagagc ccgaaggtgg ccggtgccga ggtggccgtc agctggccgg aggtggaaga
     1081 ggccctggta ctcctgcagc tctgggcaaa cctggacgtg ctactggtgg cctcttggca
     1141 ggagctgagt cggcacgtgt gcgccgttac caaggctctc gcccagtatc ccctcaagca
     1201 gtaccgggaa tcccaggcct tctccttctg cacagcaggg cgctgggcag ccggcgagcc
     1261 agtggcaaga gacggcgcag ggctgcaggc ggcctggcgg aggcagatca ggcagttcag
     1321 tcgggtcagc ccagccgtgg ctgatgcagt tgtcacagcc ttcccctccc cccgccttct
     1381 gcagcaggcg ctggaggcct gcagcacgga gcgggagcgc atgggcctcc tggccgacct
     1441 tcctgtgccg cccagtgaag gcgggcgtcc ccgcagggtg gggcctgacc tctcccgccg
     1501 catctgcctc ttcctgacca cagccaaccc tgatctcctg ctggacctgg gctcctgacc
     1561 acacgtggga ccaccaggac agcatgcagc cttggggaca gaccagacac cctgggcggt
     1621 gggggaggac ccccagccac atgtggaccc tcagcctggg tgggttctct ggctgagcag
     1681 gtctgacctc aggggaaggg tgggtggttg caggggaagt tttaggtagc tgggagaaga
     1741 ggggcttctg gctggcagat ggctggcggt tcctgtgctg agtcctgaac acgtaggccc
     1801 caggggaggc ctcagcagca gggctgtgcc cccccaacac acacacacac tcggcaggga
     1861 ccagaaggca gctccagggc cccactgcca cctggaggct tggggtgtgg caccctcagc
     1921 cagaagcagt aggggactcc caaggatgtg gaggggcagt gaggcctggg agaaggccca
     1981 ggctgctcgc aagcccggcc tctgcacgga tacgtttcag ctcacgccat gtgggtgtta
     2041 gacatcaact ctacatttat tgcagtcctt taagtctatg acggcggggc agccgctgac
     2101 agcatgcaga gcaagttagg aaaaaccgag gccctgtggg aacagcaacg cgggctccag
     2161 ccaggctctc gtcctcgcag cctcccacaa gacctggggc tcagggcagc cgcttcccca
     2221 cccagcacag cagcagaggg gccctagagc ccccacagaa aggactgtcc cagcctcggg
     2281 agcaagagat ggctccctcc ggacgggcct catccaactc cgccctggag tgtggctgga
     2341 aggaagggac agagaaagaa gggacagagg aaaggggctg tcccagccca agaaggcagt
     2401 tccactggga agtcagtcag gtccggcggc agcgcttcct ctggcagggc cgaggctcgc
     2461 gactgctggg gtgggcggag gtcgctgcct ggggatcgga cactggagcc ttgcggcggc
     2521 tgcaactcat gctcaggacc cagcccagct tgttgtgtag cacctgcttg aggcccggcg
     2581 gcagcggcag accctccagc gtgtctcccg agtctggccg cagctggcgc aggcggtggc
     2641 agcacaggta ctggagggaa gtggcgctgg cacaggagcg ggtgaccttc atgctgctcc
     2701 gggccgccgt ggagcacacc atcgggtaga atctcttgtt ctgcagcttg gtggctgcca
     2761 cacctggaag ggaggggccc agcctgaacc ccaggcaggg aaggggccag ctactgggcg
     2821 cagcccctca gacctcacgc tacagtcacc cgggtgggca gctggcacag ctgaggcaag
     2881 gcagggtgca cgcgctggcc ctgccactcc agcctggcct cactgtccca ccccctggga
     2941 ccctggggcc tcaggcttct gggaggaacc ctttcagaaa acctcagtgt cccctgggct
     3001 ggggcggggt ggctgcctcc ctcggggtga cagctgagag gagctcaagt cctgtgacgg
     3061 cctcacctat acacttcctg ttcttgaaaa aggtgagtgt gccgtgccag gtgtccaggt
     3121 gcacgccaat gatggagccc tggccgaacc gcgatgagaa gctggtcttg tcgcccttgt
     3181 ggtggaggag gcctgggggc agccagggtc gcagtgagcc cgggagctcc aggctcggcc
     3241 ccgccccacc ctgggcctca cgcacccgtg taggagaggc cccagctgtc ctcatccctg
     3301 cccagcaggc tgcagaacgt gtggcggtat ttgtccaggt ccacatccga cgtcccgatg
     3361 cccaccatct aggaacaggg gccaggcaga gggcgcgggg ctgggctgcc ggagccaggt
     3421 tccccagaag caccctgggc cgaagcaact taccatgtcg gtgccgtaga cgggagaggt
     3481 catcttgatc tcccagaagt gctggccctc ccccagctcc ttggtgcccc ggatggccgc
     3541 tgtgccgcag ctgtactcca tgtggaagct gaccttacgg ttgtcacagc tcagcagggt
     3601 ggctgatgac ttatttaagt catcccagac ccagtcgaaa tctgcaagag aggcccaggc
     3661 tggggcagcc ctgagagctc catggggctc tgccctgccc acccccaggc ccgcccgcag
     3721 tgcagtccca gcaggggctg ggccccacgc tcacactcgt cttcctctcc gcagcggcag
     3781 tccctgcccc ggtgggccga gtgcaggctg ctacagaagg aggcctcgct ctgcccagca
     3841 cagtcacaga aggactcgcc ggtcacgggc accgcactgg ggatggatgg cggcagcgtg
     3901 gagtactcgg ggtcggagtc cgagtcgctg tgctgggaga gaagggccgg ctgttactac
     3961 ctgttgcccg ctctctaccc tctcaccctt gccctctgtc cctgtcccac cctgtccctg
     4021 acccacccag gaaaggatgg gggtccaggc ctccagggac ttcacagtac cccgagcgca
     4081 cagcccaggc tccctcccaa cgggctccgg ctctgcccca ttctgcatga ccaggagggc
     4141 cctggaggcc cagccacaga gcagtagcca aggctgcctg gcctccaagt ggtttctaga
     4201 cggtggataa gccccaggtc caccccacct gcccagagct gagatggtgt ggacaccttc
     4261 cctgctaggc cagccctgca ggggccccca ggcaaggcca ccacccccac accaggccca
     4321 gctgcagcca cccggcctca gggcagtccc caccacactg tggcccaacg accttcctgt
     4381 tacctgagca gacctggctc accacagggt cctactgccc agtgcccact acaggccccg
     4441 cccctcctgg tgcaggtttc agctgtcacc tccctgaggc ttctccagcc ccttcacccc
     4501 tgttccattt ttcttctttt gcttcactga agttctcctt ggtcctgatt cttggcctcc
     4561 tcccaccaga acgtgagctc cttgtgggag gcagtgcccc tcccagcctc ctccctgtca
     4621 cagactgtgt gggggccacc caggggctga atggaccagg gggtcagcag ggagagtgct
     4681 aagtggtggc ggggggcctg tggcagaggc cttgtgacag tgagggttga gggctggtcc
     4741 agagagatga tgtgatcccc aggagaagcc acaggggacc atgacatggg cgccaccagc
     4801 ttccaggtga acgtggacct atcgaaagcc agcttcaggg caggacactg tcctttctgg
     4861 aggggatggc cccaccccac agacaaggca ggcccagaca actctacccc actgcctaga
     4921 agtgcgggtc cccagagcca gggtagaccc cctgagtcag gagcccgggc acagaactga
     4981 gcacctgcac tgcaggccca gggccgggtt ccctggagaa ggagaaagac tgaagccact
     5041 gcctacctcc tgcccacaac agactgcagg cccatttggt gacacaggac aagcagcaca
     5101 tgtgtcgaac actgccgagc ccgcaggcac gcctgcatcc cacagccacg agcctgtgag
     5161 gctcaggcat gtgacctgca ggagctggag gcgggcggga aggcaggtgg gggcctctcc
     5221 ctggctggag cagggagctc caggagacct caggacggcc cccacccggt gggtaaggac
     5281 cctgtttccc aatttgcccc caaggcctaa tggacccacc gggcttaggg agtcctcact
     5341 cagaggagag gagcccaccc ctcccaggac acctggctga gccggaacag cagcgcaggg
     5401 gagctgtgac agcagagagc agcaccaggt cacacaagca gccgccccgg aaggacacgg
     5461 cccatggtga cagaggacaa gcagcatgcg tgtgtcagac actggcctgg cctgtggaca
     5521 cgcccgcgtc ccacagccat gaccctgcga ggccaggaga gactgctcca gggcacatga
     5581 cctgtggtag ctggaagtgg ggcagggagg cgcagccaca ctcctgctct ggtccgaggg
     5641 agatctcagg aaaccgactg ggaaatcacc ccccacaggg acccccaccc ctcccagcct
     5701 cactgcgccc gtcaggccag gcagctgccc tcagggtctg ccaaggtggg ggtcaggggc
     5761 catgggggca ggtagctctg cctgcaaagc ccacaagcat gtcagatcac ctgggctgca
     5821 gacagacaaa cacctgagct gttctgaata ccttcaggtt cctggcctcc ctgagcaagt
     5881 gcagaaattt ttaccttcaa ggatcagggt ttttctgttt gtttgttttt taacacacat
     5941 atatgtgaac aaagagtatg cgtttgtact ggcagaagaa gcgtctggta agacaaccag
     6001 caagttaaca atggtcacct ccagaaatgg gctgggtaaa ccaaagaatt tttttgtttt
     6061 tgtttttttt gagtcagggt ctagctctgt cacccaggct ggaacgcact ggtgtgatca
     6121 cggctcactg cagccttgac ctccctggct caagcaatcc tcccagctca gcctcctgag
     6181 tcgttgggac tacaggcacg tgccaccacg cctgacacat tttttaaatt tttgtagaga
     6241 cagtgtttca ccatgttgcc caggcaggtc tcaaactcct gggctcaagt ggtcctccag
     6301 cttcagcctc ccaaagtgct aggattatag gtgtgagcca cagtgcccag ccccgtagtg
     6361 gagaatttct gttgaatgaa ccaaaagcaa ctgccaacct ctccatgcac catgtgtttc
     6421 agaggagaaa gcacagtgaa gaatgcagtg tgttctgagg tcctgtcacc cctgaggctg
     6481 tgtgtgtcct ttgccaaatt aaagagtctt actgaatgcg gtgcatccag gagacaggcc
     6541 caggtttgga ctggtcctct agcctcagaa gcatcttttt ctccgactga ttccgtgttc
     6601 aggtaatgtc tgcctgcctg cctagggcat ctccacacct taggccagcc acgtccgcct
     6661 cgcccgctgg aacctacctg cccatcagag tcgtagcccc agttagtgga gcctgctaga
     6721 gccacggccc gggcatctgc gtctcggcgg gctgcactca ggacgaagtg ccaggccctg
     6781 ctgttccggg ggcgtctggc catggtggaa agaatctaga agaaaacaca ggctctgggc
     6841 aaaggaagac tcacagcact cccagccctg agcttccagg ctgggccacg gacccactca
     6901 aagtggggac tgcaggggcc gcaccggtgc ccagccaggg ctccagaacg cttcaggagc
     6961 ccgtacctca ctccaggatc tgagcagctc aataaaaccc aggtagatcc tgtgaaacgc
     7021 ca
//
