LOCUS       NM_016242               3966 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens endomucin (EMCN), transcript variant 1, mRNA.
ACCESSION   NM_016242
VERSION     NM_016242.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Brookins,E., Serrano,S.E., Hyder,Z., Yacu,G.S., Finer,G. and
            Thomson,B.R.
  TITLE     Non-endothelial expression of endomucin in the mouse and human
            choroid
  JOURNAL   Exp Eye Res 247, 110054 (2024)
   PUBMED   39153592
  REMARK    GeneRIF: Non-endothelial expression of endomucin in the mouse and
            human choroid.
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Cano,I., Wild,M., Gupta,U., Chaudhary,S., Ng,Y.S.E.,
            Saint-Geniez,M., D'Amore,P.A. and Hu,Z.
  TITLE     Endomucin selectively regulates vascular endothelial growth factor
            receptor-2 endocytosis through its interaction with AP2
  JOURNAL   Cell Commun Signal 22 (1), 225 (2024)
   PUBMED   38605348
  REMARK    GeneRIF: Endomucin selectively regulates vascular endothelial
            growth factor receptor-2 endocytosis through its interaction with
            AP2.
            Erratum:[Cell Commun Signal. 2024 Jun 6;22(1):311. doi:
            10.1186/s12964-024-01696-6.. PMID: 38844932]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3966)
  AUTHORS   Wang,S., Jin,J., Chen,J. and Lou,W.
  TITLE     MUC14-Related ncRNA-mRNA Network in Breast Cancer
  JOURNAL   Genes (Basel) 12 (11), 1677 (2021)
   PUBMED   34828282
  REMARK    GeneRIF: MUC14-Related ncRNA-mRNA Network in Breast Cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3966)
  AUTHORS   Hu,Z., Cano,I., Saez-Torres,K.L., LeBlanc,M.E., Saint-Geniez,M.,
            Ng,Y.S., Argueso,P. and D'Amore,P.A.
  TITLE     Elements of the Endomucin Extracellular Domain Essential for
            VEGF-Induced VEGFR2 Activity
  JOURNAL   Cells 9 (6), 1413 (2020)
   PUBMED   32517158
  REMARK    GeneRIF: Elements of the Endomucin Extracellular Domain Essential
            for VEGF-Induced VEGFR2 Activity.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3966)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 3966)
  AUTHORS   Kanda,H., Tanaka,T., Matsumoto,M., Umemoto,E., Ebisuno,Y.,
            Kinoshita,M., Noda,M., Kannagi,R., Hirata,T., Murai,T., Fukuda,M.
            and Miyasaka,M.
  TITLE     Endomucin, a sialomucin expressed in high endothelial venules,
            supports L-selectin-mediated rolling
  JOURNAL   Int Immunol 16 (9), 1265-1274 (2004)
   PUBMED   15249540
REFERENCE   7  (bases 1 to 3966)
  AUTHORS   Kuhn,A., Brachtendorf,G., Kurth,F., Sonntag,M., Samulowitz,U.,
            Metze,D. and Vestweber,D.
  TITLE     Expression of endomucin, a novel endothelial sialomucin, in normal
            and diseased human skin
  JOURNAL   J Invest Dermatol 119 (6), 1388-1393 (2002)
   PUBMED   12485444
REFERENCE   8  (bases 1 to 3966)
  AUTHORS   Samulowitz,U., Kuhn,A., Brachtendorf,G., Nawroth,R., Braun,A.,
            Bankfalvi,A., Bocker,W. and Vestweber,D.
  TITLE     Human endomucin: distribution pattern, expression on high
            endothelial venules, and decoration with the MECA-79 epitope
  JOURNAL   Am J Pathol 160 (5), 1669-1681 (2002)
   PUBMED   12000719
REFERENCE   9  (bases 1 to 3966)
  AUTHORS   Liu,C., Shao,Z.M., Zhang,L., Beatty,P., Sartippour,M., Lane,T.,
            Livingston,E. and Nguyen,M.
  TITLE     Human endomucin is an endothelial marker
  JOURNAL   Biochem Biophys Res Commun 288 (1), 129-136 (2001)
   PUBMED   11594763
REFERENCE   10 (bases 1 to 3966)
  AUTHORS   Kinoshita,M., Nakamura,T., Ihara,M., Haraguchi,T., Hiraoka,Y.,
            Tashiro,K. and Noda,M.
  TITLE     Identification of human endomucin-1 and -2 as membrane-bound
            O-sialoglycoproteins with anti-adhesive activity
  JOURNAL   FEBS Lett 499 (1-2), 121-126 (2001)
   PUBMED   11418125
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK313504.1, AK291831.1, AC097459.3 and AL133118.1.
            
            On Nov 22, 2018 this sequence version replaced NM_016242.3.
            
            Summary: EMCN is a mucin-like sialoglycoprotein that interferes
            with the assembly of focal adhesion complexes and inhibits
            interaction between cells and the extracellular matrix (Kinoshita
            et al., 2001 [PubMed 11418125]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF205940.1, SRR1803615.175378.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000296420.9/ ENSP00000296420.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-894               AK313504.1         1-894
            895-1418            AK291831.1         627-1150
            1419-3211           AC097459.3         25700-27492         c
            3212-3966           AL133118.1         1482-2236
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q24"
     gene            1..3966
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /note="endomucin"
                     /db_xref="GeneID:51705"
                     /db_xref="HGNC:HGNC:16041"
                     /db_xref="MIM:608350"
     exon            1..172
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..894
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; endomucin-2; MUC-14; mucin-14; gastric cancer
                     antigen Ga34"
                     /codon_start=1
                     /product="endomucin isoform 1 precursor"
                     /protein_id="NP_057326.2"
                     /db_xref="CCDS:CCDS3655.1"
                     /db_xref="GeneID:51705"
                     /db_xref="HGNC:HGNC:16041"
                     /db_xref="MIM:608350"
                     /translation="MELLQVTILFLLPSICSSNSTGVLEAANNSLVVTTTKPSITTPN
                     TESLQKNVVTPTTGTTPKGTITNELLKMSLMSTATFLTSKDEGLKATTTDVRKNDSII
                     SNVTVTSVTLPNAVSTLQSSKPKTETQSSIKTTEIPGSVLQPDASPSKTGTLTSIPVT
                     IPENTSQSQVIGTEGGKNASTSATSRSYSSIILPVVIALIVITLSVFVLVGLYRMCWK
                     ADPGTPENGNDQPQSDKESVKLLTVKTISHESGEHSAQGKTKN"
     exon            173..295
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /inference="alignment:Splign:2.1.0"
     exon            296..367
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /inference="alignment:Splign:2.1.0"
     exon            368..484
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /inference="alignment:Splign:2.1.0"
     exon            485..523
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /inference="alignment:Splign:2.1.0"
     exon            524..616
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /inference="alignment:Splign:2.1.0"
     exon            617..676
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /inference="alignment:Splign:2.1.0"
     exon            677..772
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /inference="alignment:Splign:2.1.0"
     exon            773..797
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /inference="alignment:Splign:2.1.0"
     exon            798..859
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /inference="alignment:Splign:2.1.0"
     exon            860..933
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /inference="alignment:Splign:2.1.0"
     exon            934..3966
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3826..3831
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /note="hexamer: ATTAAA"
     polyA_site      3851
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /note="major polyA site"
     regulatory      3931..3936
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /note="hexamer: AATAAA"
     polyA_site      3966
                     /gene="EMCN"
                     /gene_synonym="EMCN2; MUC14"
                     /note="major polyA site"
ORIGIN      
        1 acattcacta caccttttcc atttgctaat aaggccctgc caggctggga gggaattgtc
       61 cctgcctgct tctggagaaa gaagatattg acaccatcta cgggcaccat ggaactgctt
      121 caagtgacca ttctttttct tctgcccagt atttgcagca gtaacagcac aggtgtttta
      181 gaggcagcta ataattcact tgttgttact acaacaaaac catctataac aacaccaaac
      241 acagaatcat tacagaaaaa tgttgtcaca ccaacaactg gaacaactcc taaaggaaca
      301 atcaccaatg aattacttaa aatgtctctg atgtcaacag ctactttttt aacaagtaaa
      361 gatgaaggat tgaaagccac aaccactgat gtcaggaaga atgactccat catttcaaac
      421 gtaacagtaa caagtgttac acttccaaat gctgtttcaa cattacaaag ttccaaaccc
      481 aagactgaaa ctcagagttc aattaaaaca acagaaatac caggtagtgt tctacaacca
      541 gatgcatcac cttctaaaac tggtacatta acctcaatac cagttacaat tccagaaaac
      601 acctcacagt ctcaagtaat aggcactgag ggtggaaaaa atgcaagcac ttcagcaacc
      661 agccggtctt attccagtat tattttgccg gtggttattg ctttgattgt aataacactt
      721 tcagtatttg ttctggtggg tttgtaccga atgtgctgga aggcagatcc gggcacacca
      781 gaaaatggaa atgatcaacc tcagtctgat aaagagagcg tgaagcttct taccgttaag
      841 acaatttctc atgagtctgg tgagcactct gcacaaggaa aaaccaagaa ctgacagctt
      901 gaggaattct ctccacacct aggcaataat tacgcttaat cttcagcttc tatgcaccaa
      961 gcgtggaaaa ggagaaagtc ctgcagaatc aatcccgact tccatacctg ctgctggact
     1021 gtaccagacg tctgtcccag taaagtgatg tccagctgac atgcaataat ttgatggaat
     1081 caaaaagaac cccggggctc tcctgttctc tcacatttaa aaattccatt actccattta
     1141 caggagcgtt cctaggaaaa ggaattttag gaggagaatt tgtgagcagt gaatctgaca
     1201 gcccaggagg tgggctcgct gataggcatg actttcctta atgtttaaag ttttccgggc
     1261 caagaatttt tatccatgaa gactttccta cttttctcag tgttcttata ttacctactg
     1321 ttagtattta ttgtttacca ctatgttaat gcagggaaaa gttgcacgtg tattattaaa
     1381 tattaggtag aaatcatacc atgctacttt gtacatataa gtattttatt cctgctttcg
     1441 tgttactttt aataaataac tactgtactc aatactctaa aaatactata acatgactgt
     1501 gaaaatggca atgttattgt cttcctataa ttatgaatat ttttggatgg attattagaa
     1561 tacatgaact cactaatgaa aggcatttgt aataagtcag aaagggacat acgattcaca
     1621 tatcagactg ttagggggag agtaatttat cagttctttg gtctttctat ttgtcattca
     1681 tactatgtga tgaagatgta agtgcaaggg catttataac actatactgc attcattaag
     1741 ataataggat catgattttt cattaactca tttgattgat attatctcca tgcatttttt
     1801 atttctttta gaaatgtaat tatttgctct agcaatcatt gctaacctct agtttgtaga
     1861 aaatcaacac tttataaata cataattatg atattatttt tcattgtatc actgttctaa
     1921 aaataccata tgattatagc tgccactcca tcaggagcaa attcttctgt taaaagctaa
     1981 ctgatcaacc ttgaccactt ttttgacatg tgagatcaaa gtgtcaagtt ggctgaggtt
     2041 ttttggaaag ctttagaact aataagctgc tggtggcagc tttgtaacgt atgattatct
     2101 aagctgattt tgatgctaaa ttatcttagt gatctaaggg gcagtttagt gaagatggaa
     2161 tcttgtattt aaaatagcct tttaaaattt gttttgtggt gatgtatttt gacaacttcc
     2221 atctttagga gttatataat caccttgatt ttagtttcct gatgtttgga ctatttataa
     2281 tcaaggacac caagcaagca taagcatatc tatatttctg actggtgtct ctttgagaag
     2341 gatgggaagt agaaaaaaaa aaaagaaaga aaggaaagga agagaggaga gaagaaggca
     2401 gggatctcca ctatgtatgt tttcacttta gaactgttga gcccatgctt aattttaatc
     2461 tagaagtctt taaatggtga gacagtgact ggagcatgcc aatcagagag catttgtctt
     2521 cagaaaaaaa aaaaatctga gtttgagact agcctggcca acatgttgaa accccatatc
     2581 tactaaaaat acaaaaatta gcctggtgtg gtggcgcacg cctgtagtcc cagctactct
     2641 ggagcctgag gaacgtgaat cgcttgaacc cagaagacag aggttgcagt gagctgagat
     2701 ggcactattg cactccagcc tgggtgacac agcaagactc tgtctcaaaa aaaaaaaaaa
     2761 aaaaaaggaa aaaaaagaaa gaaagaaagt cccagcacac ctagataatt taccgagctc
     2821 ttcagcaaaa accatgttac atacagcata ttccaaagaa atgaactctt ctgcaattta
     2881 aattataagt aatatgttat tttggatcct agagaaacca ttttctctac atttcatgag
     2941 catggttaga aaagagttta caagaattag gaagagggaa caattttaat ggtcagaaaa
     3001 gaataaaatt tattctagtt caagaagtgc acacaaagaa tatgcattaa tctaacaact
     3061 atgagattaa atctttcaaa aaggtcaaag gaggattgag aagtttacag agatgtccac
     3121 ggcattttat atcaatctca aaggtaaggt ctgcattttt ataaaccaac ttaaacttct
     3181 gttgagatag gatattttgt tttcaagcca aaattaccat taatcaaata tgttttaatt
     3241 atctgattta gatgatctac tttttatgcc tggcttactg taagtttttt attctgatac
     3301 acagttcaaa catcattgca acaaagaagt gcctgtattt agatcaaagg caagactttc
     3361 tatgtgtttg ttttgcataa taatatgaat ataatttaag tctatcaata gtcaaaacat
     3421 aaacaaaagc taattaactg gcactgttgt cacctgagac taagtggatg ttgttggctg
     3481 acatacaggc tcagccagca gagaaagaat tctgaattcc ccttgctgaa ctgaactatt
     3541 ctgttacata tggttgacaa atctgtgtgt tatttctttt ctacctacca tatttaaatt
     3601 tatgagtatc aaccgaggac atagtcaaac cttcgatgat gaacattcct gattttttgc
     3661 ctgattattc tctgttgagc tctacttgtg gtcattcaag attttatgat gttgaaagga
     3721 aaagtgaata tgacctttaa aaattgtatt ttgggtgatg atagtctcac cactataaaa
     3781 ctgtcaatta ttgcctaatg ttaaagatat ccatcattgt gattaattaa acctataatg
     3841 agtattctta atggagaatt cttaatggat ggattatccc ctgatctttt ctttaaaatt
     3901 tctctgcaca cacaggactt ctcattttcc aataaatggg tgtactctgc cccaatttct
     3961 agggaa
//
