LOCUS       NM_006067               1966 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens ER membrane protein complex subunit 8 (EMC8),
            transcript variant 1, mRNA.
ACCESSION   NM_006067
VERSION     NM_006067.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1966)
  AUTHORS   Pleiner,T., Tomaleri,G.P., Januszyk,K., Inglis,A.J., Hazu,M. and
            Voorhees,R.M.
  TITLE     Structural basis for membrane insertion by the human ER membrane
            protein complex
  JOURNAL   Science 369 (6502), 433-436 (2020)
   PUBMED   32439656
REFERENCE   4  (bases 1 to 1966)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1966)
  AUTHORS   Chitwood,P.J., Juszkiewicz,S., Guna,A., Shao,S. and Hegde,R.S.
  TITLE     EMC Is Required to Initiate Accurate Membrane Protein Topogenesis
  JOURNAL   Cell 175 (6), 1507-1519 (2018)
   PUBMED   30415835
REFERENCE   6  (bases 1 to 1966)
  AUTHORS   Yoshida,T., Kato,K., Yokoi,K., Oguri,M., Watanabe,S., Metoki,N.,
            Yoshida,H., Satoh,K., Aoyagi,Y., Nozawa,Y. and Yamada,Y.
  TITLE     Association of genetic variants with hemorrhagic stroke in Japanese
            individuals
  JOURNAL   Int J Mol Med 25 (4), 649-656 (2010)
   PUBMED   20198315
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 1966)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   8  (bases 1 to 1966)
  AUTHORS   Oguri,M., Kato,K., Yokoi,K., Yoshida,T., Watanabe,S., Metoki,N.,
            Yoshida,H., Satoh,K., Aoyagi,Y., Nozawa,Y. and Yamada,Y.
  TITLE     Assessment of a polymorphism of SDK1 with hypertension in Japanese
            Individuals
  JOURNAL   Am J Hypertens 23 (1), 70-77 (2010)
   PUBMED   19851296
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 1966)
  AUTHORS   Yoshida,T., Kato,K., Yokoi,K., Oguri,M., Watanabe,S., Metoki,N.,
            Yoshida,H., Satoh,K., Aoyagi,Y., Nozawa,Y. and Yamada,Y.
  TITLE     Association of gene polymorphisms with chronic kidney disease in
            Japanese individuals
  JOURNAL   Int J Mol Med 24 (4), 539-547 (2009)
   PUBMED   19724895
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   10 (bases 1 to 1966)
  AUTHORS   Bachman,N.J., Wu,W., Schmidt,T.R., Grossman,L.I. and Lomax,M.I.
  TITLE     The 5' region of the COX4 gene contains a novel overlapping gene,
            NOC4
  JOURNAL   Mamm Genome 10 (5), 506-512 (1999)
   PUBMED   10337626
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC018695.6, BP307869.1 and BC020250.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006067.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.611781.1,
                                           SRR3476690.1098539.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000253457.8/ ENSP00000253457.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AC018695.6         109435-109436       c
            3-50                BP307869.1         6-53
            51-964              BC020250.1         1-914
            965-1966            AC018695.6         88516-89517         c
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q24.1"
     gene            1..1966
                     /gene="EMC8"
                     /gene_synonym="C16orf2; C16orf4; COX4NB; FAM158B; NOC4"
                     /note="ER membrane protein complex subunit 8"
                     /db_xref="GeneID:10328"
                     /db_xref="HGNC:HGNC:7864"
                     /db_xref="MIM:604886"
     exon            1..480
                     /gene="EMC8"
                     /gene_synonym="C16orf2; C16orf4; COX4NB; FAM158B; NOC4"
                     /inference="alignment:Splign:2.1.0"
     CDS             250..882
                     /gene="EMC8"
                     /gene_synonym="C16orf2; C16orf4; COX4NB; FAM158B; NOC4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     family with sequence similarity 158, member B; neighbor of
                     COX4; COX4 neighbor"
                     /codon_start=1
                     /product="ER membrane protein complex subunit 8 isoform 1"
                     /protein_id="NP_006058.1"
                     /db_xref="CCDS:CCDS10954.1"
                     /db_xref="GeneID:10328"
                     /db_xref="HGNC:HGNC:7864"
                     /db_xref="MIM:604886"
                     /translation="MPGVKLTTQAYCKMVLHGAKYPHCAVNGLLVAEKQKPRKEHLPL
                     GGPGAHHTLFVDCIPLFHGTLALAPMLEVALTLIDSWCKDHSYVIAGYYQANERVKDA
                     SPNQVAEKVASRIAEGFSDTALIMVDNTKFTMDCVAPTIHVYEHHENRWRCRDPHHDY
                     CEDWPEAQRISASLLDSRSYETLVDFDNHLDDIRNDWTNPEINKAVLHLC"
     exon            481..557
                     /gene="EMC8"
                     /gene_synonym="C16orf2; C16orf4; COX4NB; FAM158B; NOC4"
                     /inference="alignment:Splign:2.1.0"
     exon            558..627
                     /gene="EMC8"
                     /gene_synonym="C16orf2; C16orf4; COX4NB; FAM158B; NOC4"
                     /inference="alignment:Splign:2.1.0"
     exon            628..722
                     /gene="EMC8"
                     /gene_synonym="C16orf2; C16orf4; COX4NB; FAM158B; NOC4"
                     /inference="alignment:Splign:2.1.0"
     exon            723..1966
                     /gene="EMC8"
                     /gene_synonym="C16orf2; C16orf4; COX4NB; FAM158B; NOC4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1169..1174
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC8"
                     /gene_synonym="C16orf2; C16orf4; COX4NB; FAM158B; NOC4"
                     /note="hexamer: ATTAAA"
     polyA_site      1187
                     /gene="EMC8"
                     /gene_synonym="C16orf2; C16orf4; COX4NB; FAM158B; NOC4"
                     /note="major polyA site"
     regulatory      1938..1943
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC8"
                     /gene_synonym="C16orf2; C16orf4; COX4NB; FAM158B; NOC4"
                     /note="hexamer: AATAAA"
     polyA_site      1966
                     /gene="EMC8"
                     /gene_synonym="C16orf2; C16orf4; COX4NB; FAM158B; NOC4"
                     /note="major polyA site"
ORIGIN      
        1 gctttccggc ggcgccgggc gagaagcgcg actggagtct ggggctgcgg cttcccagag
       61 gcgcgagtcg cttttccagg agccgaggcc caaacggggc cgggcctgaa gggtcccccg
      121 ccagcccgcg gccgcgccat caatcgccgc cgcctcgtcc cgcttctcgg ctgaggcgcc
      181 gcgcggccag gcagcgggtc caggcctcag ccgcgcgccc aggggcctcc ggggccctcc
      241 cgggtcagca tgcccggggt gaaactgacc acccaggcct actgcaagat ggtgctgcac
      301 ggcgccaagt acccgcactg cgccgtcaac gggctcctgg tggccgagaa gcagaagccg
      361 cgtaaggagc acctccccct gggcggcccc ggcgcccacc acaccctctt cgtggactgc
      421 atccccctct tccacggcac cctggccctc gcccccatgc tggaggtggc tctcaccctg
      481 attgattcat ggtgcaaaga tcatagctac gtgattgctg gttattatca agctaatgag
      541 cgagtaaagg atgccagtcc aaaccaggtt gcagagaagg tggcctccag aatcgccgag
      601 ggcttcagcg acactgcgct catcatggta gacaacacca agtttacgat ggactgcgta
      661 gcgcctacga tccacgtgta cgagcaccat gagaacagat ggcggtgcag agacccacac
      721 catgactact gtgaagactg gccagaggca cagaggatct cagcctcgct cctggacagc
      781 cggtcctacg agacgctcgt ggatttcgat aaccacctgg atgacattcg gaatgactgg
      841 acaaacccag agatcaataa agctgtccta cacttgtgct aggcaggcac cgctgtgact
      901 gggctccggg cctttccact acgttgaaga agaaaaccta tttttaaatg taaataaaat
      961 atcttgtagc ctgtgtggaa agctgaccgt tttaagaagt ggcatgtgcc ttgaaagggg
     1021 gcagaatgtt cagtcggtcg tgtttttaac acagagtctc tagaagaggt gcagacatcc
     1081 cgtctgactg tccctgtgga ctctctcagt tgtatgttgc tataatcctc caaatcaaag
     1141 ctctttctgc ttgtgcaaga ttgttcctat taaacagttt taactaacct tttataatct
     1201 ggagagaata ctttttaagg cttttgaact atcttttttt aaaaaaaaaa aaaaaagccc
     1261 acatcttata ctccaatgag tgtgtttgtg agtttttatc ctgtttgaat tttgatgtcg
     1321 tccaggcgtc ctgggatcag ggtagacacg tggagtgctg gtgctgagct gtttgagaag
     1381 ctctgtgtgg gtgtttctgt ggtcaggtag gctgggctgt gaacagcact gctccacatg
     1441 gtgtcggctc acagagccac cgcaactctg cccagcggga gtgacaccac tgatgccaca
     1501 gaagccttta tcgttagggg caaaagcaga catctctggt agcaggagct cagtgtggtg
     1561 ctcttcagcc acgcagaaag ctcacgtgcc tgcagggcca ggaaagggaa atgatcgttt
     1621 cttttgtatt aggaaaaaca agacttagac ttcccgagtt gtgcagagtg acgtctgtac
     1681 tttttatact tagaacattt ggtcaagttt tgtaaccaaa ccttagggaa ttgagaggga
     1741 aactttatgg tacgtttacc ttttttcctt tttatatgaa ttattttgta caaagttcag
     1801 agagcggtac ctttcctact gtcttttctt tgactccatg gaggaatttg taacgatcgg
     1861 tgtgacctga aagggtttgt gatacagcgg tcactatttt ctcttaacac ataggaaaaa
     1921 agttcagaca tcgctacaat aaatgtgctt tatttgatcc accaaa
//
