LOCUS       NM_020154               1069 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens ER membrane protein complex subunit 7 (EMC7), mRNA.
ACCESSION   NM_020154
VERSION     NM_020154.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Pleiner,T., Tomaleri,G.P., Januszyk,K., Inglis,A.J., Hazu,M. and
            Voorhees,R.M.
  TITLE     Structural basis for membrane insertion by the human ER membrane
            protein complex
  JOURNAL   Science 369 (6502), 433-436 (2020)
   PUBMED   32439656
REFERENCE   3  (bases 1 to 1069)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1069)
  AUTHORS   Bagchi,P., Torres,M., Qi,L. and Tsai,B.
  TITLE     Selective EMC subunits act as molecular tethers of intracellular
            organelles exploited during viral entry
  JOURNAL   Nat Commun 11 (1), 1127 (2020)
   PUBMED   32111841
  REMARK    GeneRIF: ER membrane protein complex subunit 4 (EMC4) and EMC7
            support SV40 infection by promoting from the late endosome (LE) to
            the endoplasmic reticulum (ER) targeting of the virus. They do this
            by engaging LE-associated Rab7, to stabilize contact between the LE
            and ER. These EMC subunits also bind to the ER-resident fusion
            machinery component syntaxin18, which is required for SV40-arrival
            to the ER.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1069)
  AUTHORS   Petko,J., Thileepan,M., Sargen,M., Canfield,V. and Levenson,R.
  TITLE     Alternative splicing of the Wnt trafficking protein, Wntless and
            its effects on protein-protein interactions
  JOURNAL   BMC Mol Cell Biol 20 (1), 22 (2019)
   PUBMED   31286866
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1069)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   7  (bases 1 to 1069)
  AUTHORS   Christianson,J.C., Olzmann,J.A., Shaler,T.A., Sowa,M.E.,
            Bennett,E.J., Richter,C.M., Tyler,R.E., Greenblatt,E.J.,
            Harper,J.W. and Kopito,R.R.
  TITLE     Defining human ERAD networks through an integrative mapping
            strategy
  JOURNAL   Nat Cell Biol 14 (1), 93-105 (2011)
   PUBMED   22119785
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1069)
  AUTHORS   Domal-Kwiatkowska,D., Glanowska,G., Smolik,S., Wilczewski,P.,
            Mazurek,U., Nowalany-Kozielska,E., Fudal,M. and Wodniecki,J.
  TITLE     [Familial hypertrophic cardiomyopathy--a case report]
  JOURNAL   Kardiol Pol 64 (11), 1287-1291 (2006)
   PUBMED   17165166
  REMARK    GeneRIF: Candidate modifier gene on the phenotypic expression of
            hypertrophy in patients with hyperthophic cardiomyopathy.
REFERENCE   9  (bases 1 to 1069)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   10 (bases 1 to 1069)
  AUTHORS   O'Brien,K.P., Tapia-Paez,I., Stahle-Backdahl,M., Kedra,D. and
            Dumanski,J.P.
  TITLE     Characterization of five novel human genes in the 11q13-q22 region
  JOURNAL   Biochem Biophys Res Commun 273 (1), 90-94 (2000)
   PUBMED   10873569
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC079203.9, AJ245874.1, BC012456.1 and AC069045.11.
            
            On Nov 22, 2018 this sequence version replaced NM_020154.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY358445.1, SRR5189655.179690.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142670, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000256545.9/ ENSP00000256545.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AC079203.9         38131-38140         c
            11-1023             AJ245874.1         23-1035
            1024-1064           BC012456.1         978-1018
            1065-1069           AC069045.11        154416-154420       c
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q14"
     gene            1..1069
                     /gene="EMC7"
                     /gene_synonym="C11orf3; C15orf24; HT022; ORF1-FL1"
                     /note="ER membrane protein complex subunit 7"
                     /db_xref="GeneID:56851"
                     /db_xref="HGNC:HGNC:24301"
                     /db_xref="MIM:620631"
     exon            1..259
                     /gene="EMC7"
                     /gene_synonym="C11orf3; C15orf24; HT022; ORF1-FL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..752
                     /gene="EMC7"
                     /gene_synonym="C11orf3; C15orf24; HT022; ORF1-FL1"
                     /note="chromosome 15 hypothetical ATG/GTP binding protein;
                     UPF0480 protein C15orf24; endoplasmic reticulum membrane
                     protein complex subunit 7"
                     /codon_start=1
                     /product="endoplasmic reticulum membrane protein complex
                     subunit 7 precursor"
                     /protein_id="NP_064539.1"
                     /db_xref="CCDS:CCDS10032.1"
                     /db_xref="GeneID:56851"
                     /db_xref="HGNC:HGNC:24301"
                     /db_xref="MIM:620631"
                     /translation="MAAALWGFFPVLLLLLLSGDVQSSEVPGAAAEGSGGSGVGIGDR
                     FKIEGRAVVPGVKPQDWISAARVLVDGEEHVGFLKTDGSFVVHDIPSGSYVVEVVSPA
                     YRFDPVRVDITSKGKMRARYVNYIKTSEVVRLPYPLQMKSSGPPSYFIKRESWGWTDF
                     LMNPMVMMMVLPLLIFVLLPKVVNTSDPDMRREMEQSMNMLNSNHELPDVSEFMTRLF
                     SSKSSGKSSSGSSKTGKSGAGKRR"
     exon            260..379
                     /gene="EMC7"
                     /gene_synonym="C11orf3; C15orf24; HT022; ORF1-FL1"
                     /inference="alignment:Splign:2.1.0"
     exon            380..518
                     /gene="EMC7"
                     /gene_synonym="C11orf3; C15orf24; HT022; ORF1-FL1"
                     /inference="alignment:Splign:2.1.0"
     exon            519..599
                     /gene="EMC7"
                     /gene_synonym="C11orf3; C15orf24; HT022; ORF1-FL1"
                     /inference="alignment:Splign:2.1.0"
     exon            600..1069
                     /gene="EMC7"
                     /gene_synonym="C11orf3; C15orf24; HT022; ORF1-FL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1005..1010
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC7"
                     /gene_synonym="C11orf3; C15orf24; HT022; ORF1-FL1"
                     /note="hexamer: AATAAA"
     polyA_site      1027
                     /gene="EMC7"
                     /gene_synonym="C11orf3; C15orf24; HT022; ORF1-FL1"
                     /note="major polyA site"
     regulatory      1042..1047
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC7"
                     /gene_synonym="C11orf3; C15orf24; HT022; ORF1-FL1"
                     /note="hexamer: ATTAAA"
     polyA_site      1069
                     /gene="EMC7"
                     /gene_synonym="C11orf3; C15orf24; HT022; ORF1-FL1"
                     /note="major polyA site"
ORIGIN      
        1 ggtctgagtg cagagctgct gtcatggcgg ccgctctgtg gggcttcttt cccgtcctgc
       61 tgctgctgct gctatcgggg gatgtccaga gctcggaggt gcccggggct gctgctgagg
      121 gatcgggagg gagtggggtc ggcataggag atcgcttcaa gattgagggg cgtgcagttg
      181 ttccaggggt gaagcctcag gactggatct cggcggcccg agtgctggta gacggagaag
      241 agcacgtcgg tttccttaag acagatggga gttttgtggt tcatgatata ccttctggat
      301 cttatgtagt ggaagttgta tctccagctt acagatttga tcccgttcga gtggatatca
      361 cttcgaaagg aaaaatgaga gcaagatatg tgaattacat caaaacatca gaggttgtca
      421 gactgcccta tcctctccaa atgaaatctt caggtccacc ttcttacttt attaaaaggg
      481 aatcgtgggg ctggacagac tttctaatga acccaatggt tatgatgatg gttcttcctt
      541 tattgatatt tgtgcttctg cctaaagtgg tcaacacaag tgatcctgac atgagacggg
      601 aaatggagca gtcaatgaat atgctgaatt ccaaccatga gttgcctgat gtttctgagt
      661 tcatgacaag actcttctct tcaaaatcat ctggcaaatc tagcagcggc agcagtaaaa
      721 caggcaaaag tggggctggc aaaaggaggt agtcaggccg tccagagctg gcatttgcac
      781 aaacacggca acactgggtg gcatccaagt cttggaaaac cgtgtgaagc aactactata
      841 aacttgagtc atcccgacgt tgatctctta caactgtgta tgttaacttt ttagcacatg
      901 ttttgtactt ggtacacgag aaaacccagc tttcatcttt tgtctgtatg aggtcaatat
      961 tgatgtcact gaattaatta cagtgtccta tagaaaatgc cattaataaa ttatatgaac
     1021 tactatacat tatgtatatt aattaaaaca tcttaatcca gaaatcaaa
//
