LOCUS       NM_016454               1019 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens ER membrane protein complex subunit 4 (EMC4),
            transcript variant 1, mRNA.
ACCESSION   NM_016454
VERSION     NM_016454.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Zhou,Y., Liu,Y., Gupta,S., Paramo,M.I., Hou,Y., Mao,C., Luo,Y.,
            Judd,J., Wierbowski,S., Bertolotti,M., Nerkar,M., Jehi,L.,
            Drayman,N., Nicolaescu,V., Gula,H., Tay,S., Randall,G., Wang,P.,
            Lis,J.T., Feschotte,C., Erzurum,S.C., Cheng,F. and Yu,H.
  TITLE     A comprehensive SARS-CoV-2-human protein-protein interactome
            reveals COVID-19 pathobiology and potential host therapeutic
            targets
  JOURNAL   Nat Biotechnol 41 (1), 128-139 (2023)
   PUBMED   36217030
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Chen,Z., Wang,C., Feng,X., Nie,L., Tang,M., Zhang,H., Xiong,Y.,
            Swisher,S.K., Srivastava,M. and Chen,J.
  TITLE     Interactomes of SARS-CoV-2 and human coronaviruses reveal host
            factors potentially affecting pathogenesis
  JOURNAL   EMBO J 40 (17), e107776 (2021)
   PUBMED   34232536
REFERENCE   3  (bases 1 to 1019)
  AUTHORS   Stukalov,A., Girault,V., Grass,V., Karayel,O., Bergant,V.,
            Urban,C., Haas,D.A., Huang,Y., Oubraham,L., Wang,A., Hamad,M.S.,
            Piras,A., Hansen,F.M., Tanzer,M.C., Paron,I., Zinzula,L.,
            Engleitner,T., Reinecke,M., Lavacca,T.M., Ehmann,R., Wolfel,R.,
            Jores,J., Kuster,B., Protzer,U., Rad,R., Ziebuhr,J., Thiel,V.,
            Scaturro,P., Mann,M. and Pichlmair,A.
  TITLE     Multilevel proteomics reveals host perturbations by SARS-CoV-2 and
            SARS-CoV
  JOURNAL   Nature 594 (7862), 246-252 (2021)
   PUBMED   33845483
REFERENCE   4  (bases 1 to 1019)
  AUTHORS   Pleiner,T., Tomaleri,G.P., Januszyk,K., Inglis,A.J., Hazu,M. and
            Voorhees,R.M.
  TITLE     Structural basis for membrane insertion by the human ER membrane
            protein complex
  JOURNAL   Science 369 (6502), 433-436 (2020)
   PUBMED   32439656
REFERENCE   5  (bases 1 to 1019)
  AUTHORS   Bagchi,P., Torres,M., Qi,L. and Tsai,B.
  TITLE     Selective EMC subunits act as molecular tethers of intracellular
            organelles exploited during viral entry
  JOURNAL   Nat Commun 11 (1), 1127 (2020)
   PUBMED   32111841
  REMARK    GeneRIF: ER membrane protein complex subunit 4 (EMC4) and EMC7
            support SV40 infection by promoting from the late endosome (LE) to
            the endoplasmic reticulum (ER) targeting of the virus. They do this
            by engaging LE-associated Rab7, to stabilize contact between the LE
            and ER. These EMC subunits also bind to the ER-resident fusion
            machinery component syntaxin18, which is required for SV40-arrival
            to the ER.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1019)
  AUTHORS   Guna,A., Volkmar,N., Christianson,J.C. and Hegde,R.S.
  TITLE     The ER membrane protein complex is a transmembrane domain insertase
  JOURNAL   Science 359 (6374), 470-473 (2018)
   PUBMED   29242231
REFERENCE   7  (bases 1 to 1019)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   8  (bases 1 to 1019)
  AUTHORS   Christianson,J.C., Olzmann,J.A., Shaler,T.A., Sowa,M.E.,
            Bennett,E.J., Richter,C.M., Tyler,R.E., Greenblatt,E.J.,
            Harper,J.W. and Kopito,R.R.
  TITLE     Defining human ERAD networks through an integrative mapping
            strategy
  JOURNAL   Nat Cell Biol 14 (1), 93-105 (2011)
   PUBMED   22119785
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 1019)
  AUTHORS   Ring,G., Khoury,C.M., Solar,A.J., Yang,Z., Mandato,C.A. and
            Greenwood,M.T.
  TITLE     Transmembrane protein 85 from both human (TMEM85) and yeast
            (YGL231c) inhibit hydrogen peroxide mediated cell death in yeast
  JOURNAL   FEBS Lett 582 (17), 2637-2642 (2008)
   PUBMED   18586032
  REMARK    GeneRIF: TMEM85 is a complex gene that encodes a novel conserved
            anti-apoptotic protein.
REFERENCE   10 (bases 1 to 1019)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BG707804.1 and AF151018.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_016454.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189655.171510.1,
                                           SRR5189655.48215.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000267750.9/ ENSP00000267750.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                BG707804.1         25-52
            29-1019             AF151018.1         1-991
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q14"
     gene            1..1019
                     /gene="EMC4"
                     /gene_synonym="PIG17; TMEM85"
                     /note="ER membrane protein complex subunit 4"
                     /db_xref="GeneID:51234"
                     /db_xref="HGNC:HGNC:28032"
                     /db_xref="MIM:616245"
     exon            1..185
                     /gene="EMC4"
                     /gene_synonym="PIG17; TMEM85"
                     /inference="alignment:Splign:2.1.0"
     CDS             100..651
                     /gene="EMC4"
                     /gene_synonym="PIG17; TMEM85"
                     /note="isoform a is encoded by transcript variant 1; cell
                     proliferation-inducing gene 17 protein; transmembrane
                     protein 85"
                     /codon_start=1
                     /product="ER membrane protein complex subunit 4 isoform a"
                     /protein_id="NP_057538.1"
                     /db_xref="CCDS:CCDS10035.1"
                     /db_xref="GeneID:51234"
                     /db_xref="HGNC:HGNC:28032"
                     /db_xref="MIM:616245"
                     /translation="MTAQGGLVANRGRRFKWAIELSGPGGGSRGRSDRGSGQGDSLYP
                     VGYLDKQVPDTSVQETDRILVEKRCWDIALGPLKQIPMNLFIMYMAGNTISIFPTMMV
                     CMMAWRPIQALMAISATFKMLESSSQKFLQGLVYLIGNLMGLALAVYKCQSMGLLPTH
                     ASDWLAFIEPPERMEFSGGGLLL"
     exon            186..300
                     /gene="EMC4"
                     /gene_synonym="PIG17; TMEM85"
                     /inference="alignment:Splign:2.1.0"
     exon            301..454
                     /gene="EMC4"
                     /gene_synonym="PIG17; TMEM85"
                     /inference="alignment:Splign:2.1.0"
     exon            455..615
                     /gene="EMC4"
                     /gene_synonym="PIG17; TMEM85"
                     /inference="alignment:Splign:2.1.0"
     exon            616..1019
                     /gene="EMC4"
                     /gene_synonym="PIG17; TMEM85"
                     /inference="alignment:Splign:2.1.0"
     regulatory      989..994
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC4"
                     /gene_synonym="PIG17; TMEM85"
                     /note="hexamer: ATTAAA"
     polyA_site      1019
                     /gene="EMC4"
                     /gene_synonym="PIG17; TMEM85"
                     /note="major polyA site"
ORIGIN      
        1 gcatttgcag agtgagacaa agcggagaac gctggtgggc ctgttgtgga gtacgctttg
       61 gactgagaag catcgaggct ataggacgca gctgttgcca tgacggccca ggggggcctg
      121 gtggctaacc gaggccggcg cttcaagtgg gccattgagc taagcgggcc tggaggaggc
      181 agcaggggtc gaagtgaccg gggcagtggc cagggagact cgctctaccc agtcggttac
      241 ttggacaagc aagtgcctga taccagcgtg caagagacag accggatcct ggtggagaag
      301 cgctgctggg acatcgcctt gggtcccctc aaacagattc ccatgaatct cttcatcatg
      361 tacatggcag gcaatactat ctccatcttc cctactatga tggtgtgtat gatggcctgg
      421 cgacccattc aggcacttat ggccatttca gccactttca agatgttaga aagttcaagc
      481 cagaagtttc ttcagggttt ggtctatctc attgggaacc tgatgggttt ggcattggct
      541 gtttacaagt gccagtccat gggactgtta cctacacatg catcggattg gttagccttc
      601 attgagcccc ctgagagaat ggagttcagt ggtggaggac tgcttttgtg aacatgagaa
      661 agcagcgcct ggtccctatg tatttgggtc ttatttacat ccttctttaa gcccagtggc
      721 tcctcagcat actcttaaac taatcactta tgttaaaaag aaccaaaaga ctcttttctc
      781 catggtgggg tgacaggtcc tagaaggaca atgtgcatat tacgacaaac acaaagaaac
      841 tataccataa cccaaggctg aaaataatgt agaaaacttt atttttgttt ccagtacaga
      901 gcaaaacaac aacaaaaaaa cataactatg taaacaagag aataactgct gctaaatcaa
      961 gaactgttgc agcatctcct ttcaataaat taaatggttg agaacaatgc ataaaaaaa
//
