LOCUS       NM_001394674            2353 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens ER membrane protein complex subunit 3 (EMC3),
            transcript variant 3, mRNA.
ACCESSION   NM_001394674
VERSION     NM_001394674.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2353)
  AUTHORS   Pleiner,T., Tomaleri,G.P., Januszyk,K., Inglis,A.J., Hazu,M. and
            Voorhees,R.M.
  TITLE     Structural basis for membrane insertion by the human ER membrane
            protein complex
  JOURNAL   Science 369 (6502), 433-436 (2020)
   PUBMED   32439656
REFERENCE   4  (bases 1 to 2353)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2353)
  AUTHORS   Chitwood,P.J., Juszkiewicz,S., Guna,A., Shao,S. and Hegde,R.S.
  TITLE     EMC Is Required to Initiate Accurate Membrane Protein Topogenesis
  JOURNAL   Cell 175 (6), 1507-1519 (2018)
   PUBMED   30415835
REFERENCE   6  (bases 1 to 2353)
  AUTHORS   Hein,M.Y., Hubner,N.C., Poser,I., Cox,J., Nagaraj,N., Toyoda,Y.,
            Gak,I.A., Weisswange,I., Mansfeld,J., Buchholz,F., Hyman,A.A. and
            Mann,M.
  TITLE     A human interactome in three quantitative dimensions organized by
            stoichiometries and abundances
  JOURNAL   Cell 163 (3), 712-723 (2015)
   PUBMED   26496610
REFERENCE   7  (bases 1 to 2353)
  AUTHORS   Christianson,J.C., Olzmann,J.A., Shaler,T.A., Sowa,M.E.,
            Bennett,E.J., Richter,C.M., Tyler,R.E., Greenblatt,E.J.,
            Harper,J.W. and Kopito,R.R.
  TITLE     Defining human ERAD networks through an integrative mapping
            strategy
  JOURNAL   Nat Cell Biol 14 (1), 93-105 (2011)
   PUBMED   22119785
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2353)
  AUTHORS   Nayak,R.R., Kearns,M., Spielman,R.S. and Cheung,V.G.
  TITLE     Coexpression network based on natural variation in human gene
            expression reveals gene interactions and functions
  JOURNAL   Genome Res 19 (11), 1953-1962 (2009)
   PUBMED   19797678
REFERENCE   9  (bases 1 to 2353)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
REFERENCE   10 (bases 1 to 2353)
  AUTHORS   Hu,R.M., Han,Z.G., Song,H.D., Peng,Y.D., Huang,Q.H., Ren,S.X.,
            Gu,Y.J., Huang,C.H., Li,Y.B., Jiang,C.L., Fu,G., Zhang,Q.H.,
            Gu,B.W., Dai,M., Mao,Y.F., Gao,G.F., Rong,R., Ye,M., Zhou,J.,
            Xu,S.H., Gu,J., Shi,J.X., Jin,W.R., Zhang,C.K., Wu,T.M.,
            Huang,G.Y., Chen,Z., Chen,M.D. and Chen,J.L.
  TITLE     Gene expression profiling in the human
            hypothalamus-pituitary-adrenal axis and full-length cDNA cloning
  JOURNAL   Proc Natl Acad Sci U S A 97 (17), 9543-9548 (2000)
   PUBMED   10931946
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC022007.5 and AC018809.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.3704919.1,
                                           SRR14243140.5453352.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000245046.7/ ENSP00000245046.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-335               AC022007.5         137357-137691       c
            336-393             AC018809.5         1643-1700
            394-487             AC018809.5         2039-2132
            488-592             AC018809.5         4601-4705
            593-674             AC018809.5         5380-5461
            675-754             AC018809.5         8428-8507
            755-837             AC018809.5         9288-9370
            838-2353            AC018809.5         14892-16407
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.3"
     gene            1..2353
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
                     /note="ER membrane protein complex subunit 3"
                     /db_xref="GeneID:55831"
                     /db_xref="HGNC:HGNC:23999"
                     /db_xref="MIM:620273"
     exon            1..335
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
                     /inference="alignment:Splign:2.1.0"
     CDS             181..966
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
                     /note="30 kDa protein; partial optokinetic response b;
                     transmembrane protein 111"
                     /codon_start=1
                     /product="ER membrane protein complex subunit 3"
                     /protein_id="NP_001381603.1"
                     /db_xref="CCDS:CCDS2594.1"
                     /db_xref="GeneID:55831"
                     /db_xref="HGNC:HGNC:23999"
                     /db_xref="MIM:620273"
                     /translation="MAGPELLLDSNIRLWVVLPIVIITFFVGMIRHYVSILLQSDKKL
                     TQEQVSDSQVLIRSRVLRENGKYIPKQSFLTRKYYFNNPEDGFFKKTKRKVVPPSPMT
                     DPTMLTDMMKGNVTNVLPMILIGGWINMTFSGFVTTKVPFPLTLRFKPMLQQGIELLT
                     LDASWVSSASWYFLNVFGLRSIYSLILGQDNAADQSRMMQEQMTGAAMAMPADTNKAF
                     KTEWEALELTDHQWALDDVEEELMAKDLHFEGMFKKELQTSIF"
     exon            336..393
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
                     /inference="alignment:Splign:2.1.0"
     exon            394..487
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
                     /inference="alignment:Splign:2.1.0"
     exon            488..592
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
                     /inference="alignment:Splign:2.1.0"
     exon            593..674
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
                     /inference="alignment:Splign:2.1.0"
     exon            675..754
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
                     /inference="alignment:Splign:2.1.0"
     exon            755..837
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
                     /inference="alignment:Splign:2.1.0"
     exon            838..2353
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1048..1053
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
                     /note="hexamer: AATAAA"
     polyA_site      1068
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
                     /note="major polyA site"
     regulatory      1666..1671
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
                     /note="hexamer: AGTAAA"
     polyA_site      1694
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
     regulatory      2330..2335
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
                     /note="hexamer: ATTAAA"
     polyA_site      2353
                     /gene="EMC3"
                     /gene_synonym="POB; TMEM111"
ORIGIN      
        1 aagtggaaga ccaggcagcc cagctgaagg cagtaagctc ggctcacagt cgcaggagag
       61 ttctggggta cacgggcaaa ggggcttgag aaggcccgga ggcgaagccg aagagaagca
      121 actgtgcccc ggagaagaga agctcgccca ttccagactg ggaaccagct ttcagtgaag
      181 atggcagggc cagaactgtt gctcgactcc aacatccgcc tctgggtggt cctacccatc
      241 gttatcatca ctttcttcgt aggcatgatc cgccactacg tgtccatcct gctgcagagc
      301 gacaagaagc tcacccagga acaagtatct gacagtcaag tcctaattcg aagcagagtc
      361 ctcagggaaa atggaaaata cattcccaaa cagtctttct tgacacgaaa atattatttc
      421 aacaacccag aggatggatt tttcaaaaaa actaaacgga aggtagtgcc accttctcct
      481 atgactgatc ctactatgtt gacagacatg atgaaaggga atgtaacaaa tgtcctccct
      541 atgattctta ttggtggatg gatcaacatg acattctcag gctttgtcac aaccaaggtc
      601 ccatttccac tgaccctccg ttttaagcct atgttacagc aaggaatcga gctactcaca
      661 ttagatgcat cctgggtgag ttctgcatcc tggtacttcc tcaatgtatt tgggcttcgg
      721 agcatttact ctctgattct gggccaagat aatgccgctg accaatcacg aatgatgcag
      781 gagcagatga cgggagcagc catggccatg cccgcagaca caaacaaagc tttcaagaca
      841 gagtgggaag ctttggagct gacggatcac cagtgggcac tagatgatgt cgaagaagag
      901 ctcatggcca aagacctcca cttcgaaggc atgttcaaaa aggaattaca gacctctatt
      961 ttttgaagac cgagcaggga ttagctgtgt caggaacttg gagttgcact taaccttgta
     1021 actttgtttg gagctggcac ctcttgaaat aaaaaggagg atgcacgagc tggcaggcat
     1081 gcagcaaggc ttgttcttgt ctgggctggg ttccccttta tgtttgaaac tagaggaaat
     1141 aggagtatgc tgggcaactt gttattttta aaatgttgta aatgttcctt ctggtgactc
     1201 tagtaatgaa agtcagagaa agggggaaac ctcaggctac tgataatata taaaatcttg
     1261 gccaggcgca gtggctcacg cctgtaatcc caggattttg ggaggctgag gcgggtgggt
     1321 cacgagatca ggagttcaag atcagcctgg ccaagatggt gaaaccccat ctctactaaa
     1381 aatacaaaaa aaaattagcc gggcgtggtg gtgggcacct gtaatcccag ctacttggga
     1441 ggctgaggca gagaactgct taaacctagg aggcggagga tgcagtgagc tgacatcgtg
     1501 ccactgcgct ccagcctggg cgacagagca aaactccatc tgaaaaaaaa aaaaaaatat
     1561 atatatatat atatatatat ctatcttagc agaagcccag tcttcgaagg aagcattgtt
     1621 aattatagct aatgattatg tgcagtcagc ctgacatcta gccacagtaa actcctgttt
     1681 tcttgggatc caaacaccct gtgttttacc attagttttg ttagtattgt taactttctt
     1741 tacacaagta aaccatttcc actaccaaag gtttagaaac tatggatgaa caaaatgaat
     1801 ctccctgttc attttgagcc tacatagaca tagagaccaa atatgtcaaa accttattca
     1861 aataaactct cttcagagtg aatacccagg gtcaggacag actcagcaaa ccttctatat
     1921 cacttaattc tgcccagtga aaatcagtga ccctgaggca ctgccagatt ctaaagtgcc
     1981 tttgggatca tcagagactg tctataaata atgcaaaatc ttgatctgga tgtcagtgta
     2041 tcaaatctta tcaaatccat tgacaaatca gtggtgctat actggaaatg gctactcctt
     2101 tggatgaagg acctcatttc ccagttgctt ggaacaaaag agaatttaca tattgctgta
     2161 agaagggaga gacggcagtt gttggtagac acacagataa gggtgcccaa gaataattct
     2221 gtctggatta aaggtcccag ggaagtgacg gtgccatttc tgtaccaaga ggtgaatata
     2281 atttgcatgt tgagtgcagt aattaagaac aaacgactcg actccacaaa ttaaaccgtc
     2341 ctgttttctc ttc
//
