LOCUS       NM_014673               3527 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ER membrane protein complex subunit 2 (EMC2),
            transcript variant 2, mRNA.
ACCESSION   NM_014673
VERSION     NM_014673.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Pleiner,T., Hazu,M., Tomaleri,G.P., Januszyk,K., Oania,R.S.,
            Sweredoski,M.J., Moradian,A., Guna,A. and Voorhees,R.M.
  TITLE     WNK1 is an assembly factor for the human ER membrane protein
            complex
  JOURNAL   Mol Cell 81 (13), 2693-2704 (2021)
   PUBMED   33964204
REFERENCE   3  (bases 1 to 3527)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 3527)
  AUTHORS   Pleiner,T., Tomaleri,G.P., Januszyk,K., Inglis,A.J., Hazu,M. and
            Voorhees,R.M.
  TITLE     Structural basis for membrane insertion by the human ER membrane
            protein complex
  JOURNAL   Science 369 (6502), 433-436 (2020)
   PUBMED   32439656
REFERENCE   5  (bases 1 to 3527)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 3527)
  AUTHORS   Guna,A., Volkmar,N., Christianson,J.C. and Hegde,R.S.
  TITLE     The ER membrane protein complex is a transmembrane domain insertase
  JOURNAL   Science 359 (6374), 470-473 (2018)
   PUBMED   29242231
REFERENCE   7  (bases 1 to 3527)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   8  (bases 1 to 3527)
  AUTHORS   Hein,M.Y., Hubner,N.C., Poser,I., Cox,J., Nagaraj,N., Toyoda,Y.,
            Gak,I.A., Weisswange,I., Mansfeld,J., Buchholz,F., Hyman,A.A. and
            Mann,M.
  TITLE     A human interactome in three quantitative dimensions organized by
            stoichiometries and abundances
  JOURNAL   Cell 163 (3), 712-723 (2015)
   PUBMED   26496610
REFERENCE   9  (bases 1 to 3527)
  AUTHORS   Ma,H., Dang,Y., Wu,Y., Jia,G., Anaya,E., Zhang,J., Abraham,S.,
            Choi,J.G., Shi,G., Qi,L., Manjunath,N. and Wu,H.
  TITLE     A CRISPR-Based Screen Identifies Genes Essential for
            West-Nile-Virus-Induced Cell Death
  JOURNAL   Cell Rep 12 (4), 673-683 (2015)
   PUBMED   26190106
REFERENCE   10 (bases 1 to 3527)
  AUTHORS   Christianson,J.C., Olzmann,J.A., Shaler,T.A., Sowa,M.E.,
            Bennett,E.J., Richter,C.M., Tyler,R.E., Greenblatt,E.J.,
            Harper,J.W. and Kopito,R.R.
  TITLE     Defining human ERAD networks through an integrative mapping
            strategy
  JOURNAL   Nat Cell Biol 14 (1), 93-105 (2011)
   PUBMED   22119785
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC021667.1 and AC022634.8.
            
            On Nov 22, 2018 this sequence version replaced NM_014673.4.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.64161.1,
                                           SRR1660807.119227.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000220853.8/ ENSP00000220853.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1238              BC021667.1         5-1242
            1239-3527           AC022634.8         35476-37764
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q23.1"
     gene            1..3527
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /note="ER membrane protein complex subunit 2"
                     /db_xref="GeneID:9694"
                     /db_xref="HGNC:HGNC:28963"
                     /db_xref="MIM:607722"
     exon            1..75
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..929
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /note="isoform 2 is encoded by transcript variant 2;
                     tetratricopeptide repeat protein 35; TPR repeat protein
                     35; tetratricopeptide repeat domain 35"
                     /codon_start=1
                     /product="ER membrane protein complex subunit 2 isoform 2"
                     /protein_id="NP_055488.1"
                     /db_xref="CCDS:CCDS6309.1"
                     /db_xref="GeneID:9694"
                     /db_xref="HGNC:HGNC:28963"
                     /db_xref="MIM:607722"
                     /translation="MAKVSELYDVTWEEMRDKMRKWREENSRNSEQIVEVGEELINEY
                     ASKLGDDIWIIYEQVMIAALDYGRDDLALFCLQELRRQFPGSHRVKRLTGMRFEAMER
                     YDDAIQLYDRILQEDPTNTAARKRKIAIRKAQGKNVEAIRELNEYLEQFVGDQEAWHE
                     LAELYINEHDYAKAAFCLEELMMTNPHNHLYCQQYAEVKYTQGGLENLELSRKYFAQA
                     LKLNNRNMRALFGLYMSASHIASNPKASAKTKKDNMKYASWAASQINRAYQFAGRSKK
                     ETKYSLKAVEDMLETLQITQS"
     exon            76..189
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /inference="alignment:Splign:2.1.0"
     exon            190..254
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /inference="alignment:Splign:2.1.0"
     exon            255..340
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /inference="alignment:Splign:2.1.0"
     exon            341..398
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /inference="alignment:Splign:2.1.0"
     exon            399..484
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /inference="alignment:Splign:2.1.0"
     exon            485..544
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /inference="alignment:Splign:2.1.0"
     exon            545..626
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /inference="alignment:Splign:2.1.0"
     exon            627..737
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /inference="alignment:Splign:2.1.0"
     exon            738..842
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /inference="alignment:Splign:2.1.0"
     exon            843..3527
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1220..1225
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /note="hexamer: AATAAA"
     regulatory      1238..1243
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /note="hexamer: ATTAAA"
     polyA_site      1249
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /note="major polyA site"
     polyA_site      1263
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
     regulatory      1811..1816
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /note="hexamer: AATAAA"
     polyA_site      1837
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
     regulatory      2124..2129
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /note="hexamer: AATAAA"
     polyA_site      2151
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
     regulatory      3502..3507
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
                     /note="hexamer: AATAAA"
     polyA_site      3527
                     /gene="EMC2"
                     /gene_synonym="KIAA0103; TTC35"
ORIGIN      
        1 gccctctcac cccgctgcct ctaggttctg ggaagatggc gaaggtctca gagctttacg
       61 atgtcacttg ggaagaaatg agagataaaa tgagaaaatg gagagaagaa aactcaagaa
      121 atagtgagca aattgtggaa gttggagaag aattaattaa tgaatatgct tctaagctgg
      181 gagatgatat ttggatcata tatgaacagg tgatgattgc agcactagac tatggtcggg
      241 atgacttggc attgttttgt cttcaagagc tgagaagaca gttccctggc agtcacagag
      301 tcaagcgatt aacaggcatg agatttgaag ccatggaaag atatgatgat gctatacagc
      361 tatatgatag gattttacaa gaagatccaa ctaacactgc tgcaagaaag cgtaagattg
      421 ccattcgaaa agcccagggg aaaaatgtgg aggccattcg ggagctgaat gagtatctgg
      481 aacaatttgt tggagaccaa gaagcctggc atgaacttgc agaactttac atcaatgaac
      541 atgactatgc aaaagcagcc ttttgtttag aggaactaat gatgactaat ccacacaacc
      601 acttatactg tcagcagtat gctgaagtta agtataccca aggtggactt gaaaacctcg
      661 aactttcaag aaagtatttt gcacaggcat tgaaactgaa caacagaaat atgagagctt
      721 tgtttggact ttatatgtcg gcaagtcata ttgcttctaa tccaaaagca agtgcaaaaa
      781 cgaaaaagga caacatgaaa tatgctagtt gggcagctag tcaaataaac agagcttatc
      841 agtttgcagg tcgaagtaag aaggaaacca aatattctct taaggctgtc gaagacatgt
      901 tggaaacatt gcagatcacc cagtcttaag gtttcaaaaa ctctttgaca ttagatttca
      961 caactgcaca attgaactta ttggcctgta acttatttac taaatgctca gtgctattta
     1021 tatactacag taattttctg ttaagaaggc agttgtaaag aatgtgttta tataaaccta
     1081 aaaatgcctt ttactgctaa gtggggagat gggggaaatc catggaagag agatttaaga
     1141 cttattgatt gtacatcagt ctcttcatat cacatataca tgtatatata taaaactcta
     1201 atgtagtata accttgttaa ataaaccatg atgatttatt aaacttgcat atgaagattc
     1261 taacttcatt ttgttatact cacagtggat atattgttta agaactcagt ttctagttaa
     1321 ctccttactg gtgatagcaa agtcacagca tatgtagctg accgaagcag attattttga
     1381 ctttgtttat attgtcgaat tggattttgc tgtacattcc agtgggctat ttgaattgtt
     1441 ttatctgctg taatatcctt ctgatgaatc ttgcttaaaa tatgtcattc tccaaagttc
     1501 agcaatattt ctttgtaaaa tttttgtttt gatataggac ataattgaga tttttacata
     1561 taaaatgcct taagataaat atgtgttacg acactttaac ttttatgcaa gttatattta
     1621 ttgaatcctt taatagtaaa ttacatgaaa gaatattaaa gaatcataaa gagtagggat
     1681 taaaggggtt gtagttattt ttaattttga aaaaaataag aaaattaaaa ctatccttag
     1741 aaacaaaaat gttaaaattt tcctattcta tcattacctt agttagaatt ttttattttt
     1801 ctcttttcat aataaacaac cattatgcta ccttcaacag tatttgacat gtttcaacaa
     1861 aatagaagag acttgaaagt taaatttcat ttagacacaa atcagttttt aaaatagcaa
     1921 ctgttcaccg caaatattta tggtatttgt ttataataaa tatatttaaa tttattttta
     1981 ctaaaaatat acagaggcat ctacagactt gtcattgctg ctttggacaa gtggggattg
     2041 tggaggaatt gacaatctag aagtgtttag aaagaaaagt attgtcaata tcagcttctt
     2101 ttaacctttt gaatagttct gctaataaat tattctccat atctagtcta atctttcata
     2161 ttacagtcat tttcgtttta ttctgccacg agtaataggc agctgattaa tatattctgt
     2221 gaaagaactc ttctcatctg aaggtaattc tctactaatc tttattacca caaagatccc
     2281 gtctttgtac acatgaaatt agtaactgca ttttcaacat gatgaaccat ttgaaattta
     2341 cattttctgt aaagaaaata gtgcctgcac gttgtttcac taccttttcc ccttcaaacc
     2401 tcctccccgt cagtcactgg attagtgttt actctagcaa ttatatttgt gtaataagta
     2461 attcagtgcc tcttatagct gtctgcctta gtttcacttt tttttttttt tttttgagac
     2521 agtctcgttc tgtcacccag gctggagtgc agtgacgtga tcgcagctca ctgcaacctc
     2581 ctcccgggtt caagcgattc ttgtgcctca gcctctgagt agctgggact acaggcatgt
     2641 gccaccatgc ctggctaatt ttgtattttt agtagagatg gggtttcacc atgttggcca
     2701 ggctggtctg aaactcctgg cctcaagtga tctacccgcc tcagcctccc gaaatgctgg
     2761 gattacaggc atgagccact gagcctggcc agtatcactt tattttttca aaattttaat
     2821 aacttctttc caagatttta tatttgttaa gcacattgcc aatccttagt tatttataaa
     2881 cttcaactta aatgtactga taatcccatt acagcaggca gttgcctaca gctattacct
     2941 ttatttcttc tttagcagta ccttagtttg atctgatagt ggcaaggatc cctcttcctc
     3001 aaagagatta ggaccttacc ccaactccag aggttgcacc ttcattgatc tgaaacagaa
     3061 gtggtagttt aattctgtat agtctgatta gtacgaggca gaactttttg cagtgaagga
     3121 aatcaaccat ccattctgtc tctctattgt ggtagctact aaccatatgt gatattgagt
     3181 acttgatatg tgactactgc aattgagaaa ttgaatgaaa tgaacagaac cctactgaga
     3241 agggctttcc tttcaaatta aaaaatcaga gctttgtgtg gtaacagtca ttgcctcttt
     3301 ctcctctttc tggccaacag catgcctaaa ggtgcagctg ccatcaaaca accatgaaac
     3361 agcaaatgtg agaatgaaag ttacatttca gagatagaac tgaaggagga ttaaggggat
     3421 cctgggtccc tgatatggca ttgttgaaca cccaaatcaa gatgagcaat tgccaaactc
     3481 cccaattatt aaatgagaaa aaataaactc cagattgttt aagacac
//
