LOCUS       NM_206538               9439 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens ER membrane protein complex subunit 10 (EMC10),
            transcript variant 2, mRNA.
ACCESSION   NM_206538
VERSION     NM_206538.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Wang,X., Li,Y., Qiang,G., Wang,K., Dai,J., McCann,M., Munoz,M.D.,
            Gil,V., Yu,Y., Li,S., Yang,Z., Xu,S., Cordoba-Chacon,J., De
            Jesus,D.F., Sun,B., Chen,K., Wang,Y., Liu,X., Miao,Q., Zhou,L.,
            Hu,R., Ding,Q., Kulkarni,R.N., Gao,D., Bluher,M. and Liew,C.W.
  TITLE     Secreted EMC10 is upregulated in human obesity and its neutralizing
            antibody prevents diet-induced obesity in mice
  JOURNAL   Nat Commun 13 (1), 7323 (2022)
   PUBMED   36443308
  REMARK    GeneRIF: Secreted EMC10 is upregulated in human obesity and its
            neutralizing antibody prevents diet-induced obesity in mice.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9439)
  AUTHORS   Liu,L., Mao,S., Chen,K., Dai,J., Jin,S., Chen,L., Wang,Y., Guo,L.,
            Yang,Y., Zhan,C., Xiong,Z., Diao,H., Zhou,Y., Ding,Q. and Wang,X.
  TITLE     Membrane-Bound EMC10 Is Required for Sperm Motility via Maintaining
            the Homeostasis of Cytoplasm Sodium in Sperm
  JOURNAL   Int J Mol Sci 23 (17), 10069 (2022)
   PUBMED   36077468
  REMARK    GeneRIF: Membrane-Bound EMC10 Is Required for Sperm Motility via
            Maintaining the Homeostasis of Cytoplasm Sodium in Sperm.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9439)
  AUTHORS   Kaiyrzhanov,R., Rocca,C., Suri,M., Gulieva,S., Zaki,M.S.,
            Henig,N.Z., Siquier,K., Guliyeva,U., Mounir,S.M., Marom,D.,
            Allahverdiyeva,A., Megahed,H., van Bokhoven,H., Cantagrel,V.,
            Rad,A., Pourkeramti,A., Dehghani,B., Shao,D.D., Markus-Bustani,K.,
            Sofrin-Drucker,E., Orenstein,N., Salayev,K., Arrigoni,F.,
            Houlden,H. and Maroofian,R.
  TITLE     Biallelic loss of EMC10 leads to mild to severe intellectual
            disability
  JOURNAL   Ann Clin Transl Neurol 9 (7), 1080-1089 (2022)
   PUBMED   35684946
  REMARK    GeneRIF: Biallelic loss of EMC10 leads to mild to severe
            intellectual disability.
REFERENCE   4  (bases 1 to 9439)
  AUTHORS   Haddad-Eid,E., Gur,N., Eid,S., Pilowsky-Peleg,T. and Straussberg,R.
  TITLE     The phenotype of homozygous EMC10 variant: A new syndrome with
            intellectual disability and language impairment
  JOURNAL   Eur J Paediatr Neurol 37, 56-61 (2022)
   PUBMED   35124540
  REMARK    GeneRIF: The phenotype of homozygous EMC10 variant: A new syndrome
            with intellectual disability and language impairment.
REFERENCE   5  (bases 1 to 9439)
  AUTHORS   Coukos,R., Yao,D., Sanchez,M.I., Strand,E.T., Olive,M.E.,
            Udeshi,N.D., Weissman,J.S., Carr,S.A., Bassik,M.C. and Ting,A.Y.
  TITLE     An engineered transcriptional reporter of protein localization
            identifies regulators of mitochondrial and ER membrane protein
            trafficking in high-throughput CRISPRi screens
  JOURNAL   Elife 10, e69142 (2021)
   PUBMED   34414886
  REMARK    GeneRIF: An engineered transcriptional reporter of protein
            localization identifies regulators of mitochondrial and ER membrane
            protein trafficking in high-throughput CRISPRi screens.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9439)
  AUTHORS   Christianson,J.C., Olzmann,J.A., Shaler,T.A., Sowa,M.E.,
            Bennett,E.J., Richter,C.M., Tyler,R.E., Greenblatt,E.J.,
            Harper,J.W. and Kopito,R.R.
  TITLE     Defining human ERAD networks through an integrative mapping
            strategy
  JOURNAL   Nat Cell Biol 14 (1), 93-105 (2011)
   PUBMED   22119785
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 9439)
  AUTHORS   Junes-Gill,K.S., Gallaher,T.K., Gluzman-Poltorak,Z., Miller,J.D.,
            Wheeler,C.J., Fan,X. and Basile,L.A.
  TITLE     hHSS1: a novel secreted factor and suppressor of glioma growth
            located at chromosome 19q13.33
  JOURNAL   J Neurooncol 102 (2), 197-211 (2011)
   PUBMED   20680400
  REMARK    GeneRIF: High-expression of hHSS1 is associated high-grade gliomas.
REFERENCE   8  (bases 1 to 9439)
  AUTHORS   Wang,X., Gong,W., Liu,Y., Yang,Z., Zhou,W., Wang,M., Yang,Z.,
            Wen,J. and Hu,R.
  TITLE     Molecular cloning of a novel secreted peptide, INM02, and
            regulation of its expression by glucose
  JOURNAL   J Endocrinol 202 (3), 355-364 (2009)
   PUBMED   19570817
  REMARK    GeneRIF: as a novel secreted protein, INM02 is associated with
            functions of pancreatic islets, especially of beta-cells
REFERENCE   9  (bases 1 to 9439)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   10 (bases 1 to 9439)
  AUTHORS   Umair,M. and Alfadhel,M.
  TITLE     EMC10-Related Neurodevelopmental Disorder
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   37319253
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            HY107516.1, HY149334.1 and AC020909.9.
            
            On Jun 2, 2019 this sequence version replaced NM_206538.3.
            
            Transcript Variant: This variant (2) lacks an exon in the 3' coding
            region that results in a frameshift compared to variant 1. The
            resulting protein (isoform 2) is longer and has a distinct
            C-terminus compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY761097.1, SRR6380201.4334.7
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000334976.11/ ENSP00000334037.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-475               HY107516.1         32-506
            476-807             HY149334.1         140-471
            808-9439            AC020909.9         80546-89177
FEATURES             Location/Qualifiers
     source          1..9439
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..9439
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /note="ER membrane protein complex subunit 10"
                     /db_xref="GeneID:284361"
                     /db_xref="HGNC:HGNC:27609"
                     /db_xref="MIM:614545"
     exon            1..152
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..827
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; hematopoietic signal peptide-containing
                     membrane domain-containing 1; hematopoietic signal
                     peptide-containing secreted 1; UPF0510 protein INM02"
                     /codon_start=1
                     /product="ER membrane protein complex subunit 10 isoform 2
                     precursor"
                     /protein_id="NP_996261.1"
                     /db_xref="CCDS:CCDS12796.1"
                     /db_xref="GeneID:284361"
                     /db_xref="HGNC:HGNC:27609"
                     /db_xref="MIM:614545"
                     /translation="MAAASAGATRLLLLLLMAVAAPSRARGSGCRAGTGARGAGAEGR
                     EGEACGTVGLLLEHSFEIDDSANFRKRGSLLWNQQDGTLSLSQRQLSEEERGRLRDVA
                     ALNGLYRVRIPRRPGALDGLEAGGYVSSFVPACSLVESHLSDQLTLHVDVAGNVVGVS
                     VVTHPGGCRGHEVEDVDLELFNTSVQLQPPTTAPGPETAAFIERLEMEQAQKAKNPQE
                     QKSFFAKYWMYIIPVVLFLMMSGAPDTGGQGGGGGGGGGGGSGR"
     exon            153..225
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /inference="alignment:Splign:2.1.0"
     exon            226..335
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /inference="alignment:Splign:2.1.0"
     exon            336..440
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /inference="alignment:Splign:2.1.0"
     exon            441..622
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /inference="alignment:Splign:2.1.0"
     exon            623..716
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /inference="alignment:Splign:2.1.0"
     exon            717..9439
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1703
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
     regulatory      1892..1897
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /note="hexamer: ATTAAA"
     polyA_site      1919
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /note="major polyA site"
     regulatory      1943..1948
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /note="hexamer: TATAAA"
     polyA_site      1978
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
     regulatory      3020..3025
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /note="hexamer: TATAAA"
     polyA_site      3042
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
     regulatory      3141..3146
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /note="hexamer: ATTAAA"
     polyA_site      3187
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
     regulatory      5411..5416
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /note="hexamer: AGTAAA"
     polyA_site      5437
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
     regulatory      6967..6972
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /note="hexamer: AAGAAA"
     polyA_site      6987
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
     polyA_site      7712
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
     regulatory      9410..9415
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /note="hexamer: AATAAT"
     regulatory      9419..9424
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
                     /note="hexamer: AATAAA"
     polyA_site      9427
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
     polyA_site      9439
                     /gene="EMC10"
                     /gene_synonym="C19orf63; HSM1; HSS1; NEDDFAS"
ORIGIN      
        1 gctcacagcc gtcccttcgc tggtgggaag aagccgagat ggcggcagcc agcgctgggg
       61 caacccggct gctcctgctc ttgctgatgg cggtagcagc gcccagtcga gcccggggca
      121 gcggctgccg ggccgggact ggtgcgcgag gggctggggc ggaaggtcga gagggcgagg
      181 cctgtggcac ggtggggctg ctgctggagc actcatttga gatcgatgac agtgccaact
      241 tccggaagcg gggctcactg ctctggaacc agcaggatgg taccttgtcc ctgtcacagc
      301 ggcagctcag cgaggaggag cggggccgac tccgggatgt ggcagccctg aatggcctgt
      361 accgggtccg gatcccaagg cgacccgggg ccctggatgg cctggaagct ggtggctatg
      421 tctcctcctt tgtccctgcg tgctccctgg tggagtcgca cctgtcggac cagctgaccc
      481 tgcacgtgga tgtggccggc aacgtggtgg gcgtgtcggt ggtgacgcac cccgggggct
      541 gccggggcca tgaggtggag gacgtggacc tggagctgtt caacacctcg gtgcagctgc
      601 agccgcccac cacagcccca ggccctgaga cggcggcctt cattgagcgc ctggagatgg
      661 aacaggccca gaaggccaag aacccccagg agcagaagtc cttcttcgcc aaatactgga
      721 tgtacatcat tcccgtcgtc ctgttcctca tgatgtcagg agcgccagac accgggggcc
      781 agggtggggg tgggggtggg ggtggtggtg ggggtagtgg ccggtgaggg cccaggctgg
      841 tcagcgtccc gtcttgcaca cccaggggcc tccctttctg ctggagtccc ctgtgtcctc
      901 agccatccca agaagggttt gctggtccct cctttccccc cgtcccacga ggccacctgg
      961 gccagcccct tgtcctctgc cttctgctgg cagaggagca gctggactgg ggcctttggc
     1021 acagcagccg gtgtctcctg cgcccgcctc ccccatggcc ccatgcagcc ccaggggctt
     1081 cccccctgcc catggagtag agcccgagat cctggccact atgccagttc tgacctcgca
     1141 tccccctacc ccgagcccat gcagtctggg aacatgccgc cttctctcca gcctctgtgc
     1201 ctttgttcca ggtggtctca ccctcctgtc cctggctggg ctaggtggtc ctgtccaggc
     1261 tcctgcagcg cccccctcac tttgacactg gactaggatg cagcctccct tctgtgtccc
     1321 cttgagggta ccctgggtcc cctcatcagg ggcagaggca tgaaagagtc ggggctggat
     1381 ggccgggggc ttctgggccc gacgcctagt gcagcccctg gggtcgtggt ttgacatttg
     1441 tctgcctggt gcaaacaagg aatccttgcc tttaaggtga caggccctcc acaggcttcc
     1501 agacttgaag gaaaaggttt aagaaagaaa acaaaaccaa cagttagtgg agtcaaagcc
     1561 cagacactgt aaatagaacc ccctccacca ccccccgccg cccagcatcc tacctggact
     1621 gcggtgctac gagggcctgc gggcctttgc tgtgtgccac cctccctgta agtctattta
     1681 aaaacatcga cgatacattg aaatgtgtga acgttttgaa aagctacagc ttccagcagc
     1741 caaaagcaac tgttgttttg gcaagacggt cctgatgtac aagcttgatt gaaattcact
     1801 gctcacttga tacgttattc agaaacccaa ggaatggctg tccccatcct catgtggctg
     1861 tgtggagctc agctgtgttg tgtggcagtt tattaaactg tcccccagat cgacacgcag
     1921 ctagcctcct gcattgtatg gttataaata gcaccctagt gagtgctgtc gtccagtctc
     1981 tcttgggtgt ggtttgaatg atctcgggat gcatgactaa aatggaatta cgatggcaga
     2041 tcggaaacgc gtttataaac aagtgatggt ttgtgagtcg actgtcaaga ccaggaggtg
     2101 gtgcaccgtg ggaggttgcc ttccctccca tcacttcttt ttgtttttga gtcagggtct
     2161 tgctctgtta cccaagctgg agtgcagtgg cggtaccttg actcactgcg acctccacct
     2221 ctcaggctca gcgattctcc cacctcagcc tcctgagtag ctggaattgt aggcgtgcac
     2281 caccacgcgc agctagtttt tgtatttttt gtagagacgg ggtttcacca tattgcccag
     2341 gctggtcgtg aactgctgga ctcgagtgat ccacctgcct cggcttccca aagtgctggg
     2401 attacaggcg tgagccaccg cgcctggcct atttttggta actttaataa agaagatagt
     2461 cttagcattg ctaacttctg tttctttctc cacctgcagg agatagctta tgctttttct
     2521 gtcttcattc catgtgaatc ctgcttcatt cttttcctag aggattccag aaatatttac
     2581 taatgctttt tttttttttt tttttttttt tttgaggcag agtctcgctc tgtcatccag
     2641 gctggagtac aatggcacga tctcagctca ctgcaacctc cgcttcctga gtttaagcga
     2701 ttctcctgca tcagcctccc aaatagcggg gattgcaggc gcccgccaac acacctggct
     2761 aatttttgta ttgttagtag agacagggtt tcaccatgtt ggccaggcta gtcttgaact
     2821 cctgacctca ggtgatctac ctgcctcagc ttcccaaagt gctgggatta caggcatgag
     2881 ccactgcgcc aggcctactg atgctttttg ggaagtgttc ccaacttgga gtcctggtcc
     2941 ctgtgtctga tctgcagatg tcttctaccc cggcactggg ggaacatctt atttttcctg
     3001 ggttaaacat tgggatcttt ataaacctga ttatgtcctt tccctctaag tctcggttgc
     3061 tttcaggctg gcagggttac tctgttgggt attccaggaa atgcctgagc tccttcaggc
     3121 tcccctagcg ttctgttctt attaaacttt tcttccccgt ctggccggtc cttggggaac
     3181 ccactcacta ttttatttta ttgaccgggt tatccaggga tgaagtatga aactcaaaag
     3241 atagagtagg ttggccaggc atagtgattc atgcttgtag tcccagctgt ttgggaggct
     3301 gagacaggag gatcgcttga gccccagagt tcgatgcttc ctgagctgtg attgaatcac
     3361 tttactccag cctgggtgac agagtgagac cctgtctcca gaatagacaa aagatgcagt
     3421 ggaatccacg tccagaaact gtatgctcac tctgggctcc agcacccact tcccctccct
     3481 ggaggcagcc tgagttttct gtcccctggt atgctgcaga tactctccac agaaacaagc
     3541 agatgcacat gtcaacagtt ttgcaagtgt tgctcttttc ccccaccttc ttttgacata
     3601 gctggggcag tttccatctc tggtataagg tgccatctta ttccatctct ggtgtaagag
     3661 tgtgttgctg gatttgagta gttctaagca gcactgcagg agtccccctg ttctgctgca
     3721 tcattttgca cacatatgtg tctgtggaag acatggcccg gtggggcccc accttgctgg
     3781 gggagaggga tgtgtgtgtg atggtggtgc atgcttcccc cttgctactc caggtgtctg
     3841 ccccatccct tcaccctgga ggttccagac agcccctctt caccagcgat tccccaactc
     3901 atctccagct cagatcccac ccctgatctc cagactcatg gccccagctg cctcctgggc
     3961 tccttcccca ggatgtgcct caggggccac taaaggagcc agtgcctccc acctgcccgg
     4021 atcgggtgct cctccaggga tctttgttcc agtgagtggt gccatcacct gcccaacctc
     4081 atggaccaga gcactgccct gtcccaatca cgttctccta tgcaccacca tacctacccc
     4141 taaagttctt cctggcctca tcccgtcacc tctgtctcca tggccttggt cccagctcaa
     4201 gcctcgtcct ctgccacctg gaccatcggc ccagcctcct ctttggcctc ccagccccca
     4261 acatcccccc ctcagtccat ccccctaatg gagagcttcc tgacactaga gctgacctca
     4321 ctactcccct gctcacagcc ctccgcgtgg cctctcacct cccccaggat agagtcccaa
     4381 cccatccttc tgtgtctcct cctctctgtg tccactgtcc tgaccctaac tcaatcctca
     4441 ttctctgcct cctggaccct catcctagcc tcgtgacttc tggtctaaga tgtgagagca
     4501 cctggcaagg taaatgctcc aggaggggtt gctccctccc atttcctgcc accagaaaat
     4561 gttatcggct gcgtggcatt gcgaaagcct tcctcttccc agcctagggt ttcatgtggg
     4621 catttaggtg ctcccagtac atagttgcat ggcaaacaat cctaaaatat acaagactac
     4681 atttagtatt ttgtaaaatt ctttactaac cccaaattgt ctactggaaa ctataggctc
     4741 cttttttttt ggtgggtggg gggcaggtgt gtttgtggga tggagtttca cttgtcaccc
     4801 aggctggagt gcagtggctc aattttggct caatgcaacc tccacctccc aggttcaagc
     4861 aattctcttg cctcagcctc ctgagtagct gggattacaa ccgtgtgcca ccacacccag
     4921 ccaatttttg tatttttagt agagaccggg tttcaccatg ttggccaggc tggtcttgaa
     4981 ctcctgacct caagcgatcc accttagtct cctaaagtgc tgggattaca tgtgtgagcc
     5041 accgcccctg gcctctgtag gcttctttaa cccatcagag agactccctg gtaaggatta
     5101 ggggctaatt actggtgcct cttcagctgt cagtccatcc tcctttattt gtacccacct
     5161 tccagtcagc aaggcacaac aatgttgtta caaaggaaac aaactcaatt tggcccattt
     5221 cctggaaatg caatctccca aacaggtttg tgtgaaccaa attccatttt cctttgtttc
     5281 ccatgtgcag tcccagttgc atttccatgg gagccaatcc acccacccat agaatgactt
     5341 gaaaatgtgt gaagttcaga agtttcatac caagaagttt gctgagcatg atgtttgtat
     5401 attgattagg agtaaaaatg ttcattttta caaaatatta caacttctcc ctctcccctc
     5461 cttccaccca gtatcccctg ttcctccccc aggcattctg gtcactgttc ctttttgttg
     5521 agatcattct cggtgtctca tatagctggg ttgcagctcc gttctttgcc aggaggtggt
     5581 aatagaggat tccacgcact cagaatgcct caggccggcg cctgtggctt ctctcaatgg
     5641 agggaagtgt cctgcagggg gcgctgttgg tccttcaggc aaggtcgggt tccctcgaag
     5701 tctctgtctt agcagttgct ggtcttcaga aacattgggc accaattttc tcatttctga
     5761 aatggaattc aggtgaggtc ccctcagtgc tgaggtgctt catgttgtca gaggaagagt
     5821 tgggaaggga gcggaatgga gcagaaaggc gtcatccgct gctcactgag ctcagatagg
     5881 aggccaggga tcggcagatt caggagccca ctcctggcca accccagggg ccccacccag
     5941 tcctgtccct gctccttacg tgctgtgtat ctttaggcaa gttgcttcct gtctctgagc
     6001 gtcgtctttg tttccgcctt cctgggccta cggcaggcct gattcctcca ggcagtgtcc
     6061 agagttccgg acagatggaa aaggaagaga gaatgggtgt cacaaaggtc tgtccactcc
     6121 tgggcctcag gcttgggggc cctgctcagg gctagcctgt ttgggcctcg gtatggactc
     6181 ctgcctcctg gtcaggacct ggaaacctgg gagcaggcaa ggcaggaaca ggagagggag
     6241 gcaaaggccc accaggcagg gatgcagggg ccacctggag acggaggaga cggggctccc
     6301 gggagggagg ccagtgggag tgagagcttg aaggagagag agccaggaga ggagacaggt
     6361 ccagagagga gggaaaggaa aggacagggc agagacagag aaaccagaga gagggaagga
     6421 agagagaccc aagggggaga gacagggaag gaagagggag gtgagaagag gaagaggcag
     6481 attcagagga gagaggagag aaaagaggtg gaaatcaaac aggagaaaga gggaggagag
     6541 gagggaggag agagtgtagg aaaaaagcag gaggaaagag ccgggcacgg tggctcacgc
     6601 ctgtaatccc agcactttgg gaggccaagg caggcagatc acgaggtgag gagatcgaga
     6661 ccgtcctggc taacacggtg aaaccctgtc tctactaaaa atacaaaaaa aaaattagca
     6721 gggcatggtg gcaggcgcct gtagtcccag ctactcggga ggctgaggca ggagaatggc
     6781 gtgaaccctg gaggcggagc ttgcagtgag ccgagattgc gccactgcac tccagcctgg
     6841 gcgacagagc gagactccgt ctcaaaaaaa aaaaaaaaaa aaaaaagaaa agatggccag
     6901 agcaggaggg gagagaggaa gagggagaaa gaaggaaaca gtgtaggggg tcagaagtgg
     6961 aaagagaaga aaaaacagaa gaggaaagaa agacgagggg gtgaaaaaag ggacaggaag
     7021 gaagagaggg agagggtggg tgccatggct ggtgagctcg gagaagaggg aagcagagaa
     7081 acgggtgaag gagggagcgt cctagggaga agtgggtggg ggagagggtg ttgggggaga
     7141 agagggtggg ggagaggggg ccccgggaga gggagggatg gaggagcggg gagctccagc
     7201 cagagaagag gaaagagaaa ggagagagct aggaactggg aaggaaaaga gctaggagga
     7261 gaccatgaga cagaaagaga agagggtgaa gagagaggat agtggctggg aggagagagg
     7321 aggagggagg agcacagagg gagaggagag gaaacagcca gagaatctgc tagaagggag
     7381 agaggagaga gaagagccgg acagggagag cagaagtgag aaaaaggagg gtggccacag
     7441 agaggacagc gagaaaaaga gaacaggaag aggggaaaag agaagaaatg ggagagggaa
     7501 gaagggtggg agagaaaacg gaacagagag ggggcacaga cagcggactg gaccgagagg
     7561 gagatggagc ggtgcggagg gggagagaga aagagggagg gaaggagggt aggagagggc
     7621 tggagatagg ggaaaagaga aaaggagggt aggagagggc tggagagaga agggaagtta
     7681 agaagggaag gagagagaca gacattaggg aagaaggaaa ggagaaagga aaaaatacaa
     7741 aaaagggcag agagaaaaga acaggccggg catggtgcct cgcacctgtg gtcccagcac
     7801 tttgagaggc taaggcagga ggatcactgg agcctgggag gtggaggctg cactactgca
     7861 ctccagcggg tgagtgagcg agaaaaaaag cagaaaggaa agaagaggtg catgagtgtg
     7921 gaagagcaat atggagagaa agaagggaga gaggattgga agtagcgaca gaggaccatg
     7981 cccctccagc tgaggagggg gcgcccctaa gacagcgtca gcccagagac cggggcagga
     8041 agcaggaacg tgaagcaggt taggagccag tgtgaggcac ggagagggcc gggtagctgg
     8101 gcggcggggg tgtcgtgggc acagcccagg ctactgcggg gacaggctgc cagacccggc
     8161 gggtccaggt tgggaactcg gccatgcagc agaaacgttc cctgcctcaa cagggcgcag
     8221 ccaccccggg gccatctctg tgacccaggg agagaaagga caacgaatgg gactagacga
     8281 ggatggggag gaacagagca gctgtggaag gtgcggcaga gaagggccca ggagaaagga
     8341 ggaagggaag gagctggagg ggcgggagaa caggagacag aacaggacag agacagctgc
     8401 ccgggaggat gggagaacag aaagagggag gaaacgccga gcactgacct gggggagggg
     8461 agtaaagaga agtgaagggg gattggaagg gaactggaga atgagagaag caacaggcgg
     8521 ggtgcgtgta ggagggcggg agagccaatg acaagacaga aaaggcagag aaagcaaagc
     8581 aagaccagac tcctcatccg gtaacactgt gtcaggtcat tgccctccca ccccgccccc
     8641 aaccccataa ctgaaaacaa gtaggaacct ggataaaata gtcttaacaa tttttttttt
     8701 gagacggagt cttgctgtgt tgcccaagct agagtgcagt ggcgcgatct cggctcactg
     8761 caaactccgc ctcccgggtt taagcgattc tcctgcctca gcctcccaag tagctgggat
     8821 tacaggggcc cgccatcgtg cctgctaatt tttgtatttt ttagtagaga cggggtttca
     8881 ccattttggc taggctggtc tcgaattcct gacctcatga tccgcccacc ttggcctccc
     8941 aaagtgctgg gattacaggc gttgagccac cgtggccggc ccttaaaagc atcaaactac
     9001 atacaagacc gtaaggaagc tgtgggtcaa cgcggaggga cgtgggaacc catcccggtg
     9061 aagcggcagc gtcacgtcct ttctcccagg gatctgttgg tcggagacat ctggactttc
     9121 atgtggacag cctgagtgac agggggaccc aggggcctca cctgggctac ctggggtggg
     9181 tggaccctcc actgtgccaa ggtgcagcct gggctcggag gaacatgccc tccgcctagt
     9241 ccctgacatc cttttcacat ccaacacgca gagcaggggc gtgagggagc aggcaagcag
     9301 ggaagggaaa gacaatgagc aagagccagc gggacggtgg ggcggatggc agaggcttcg
     9361 ctgctggaaa atcagacgca gagtacgaaa cgcctgtgct cactatgtta ataataataa
     9421 taaaaggcaa aaaaccagg
//
