LOCUS       NM_003640               5913 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens elongator acetyltransferase complex subunit 1 (ELP1),
            transcript variant 1, mRNA.
ACCESSION   NM_003640
VERSION     NM_003640.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Chaverra,M., Cheney,A.M., Scheel,A., Miller,A., George,L.,
            Schultz,A., Henningsen,K., Kominsky,D., Walk,H., Kennedy,W.R.,
            Kaufmann,H., Walk,S., Copie,V. and Lefcort,F.
  TITLE     ELP1, the Gene Mutated in Familial Dysautonomia, Is Required for
            Normal Enteric Nervous System Development and Maintenance and for
            Gut Epithelium Homeostasis
  JOURNAL   J Neurosci 44 (37), e2253232024 (2024)
   PUBMED   39138000
  REMARK    GeneRIF: ELP1, the Gene Mutated in Familial Dysautonomia, Is
            Required for Normal Enteric Nervous System Development and
            Maintenance and for Gut Epithelium Homeostasis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Riccardi,F., Romano,G., Licastro,D. and Pagani,F.
  TITLE     Age-dependent regulation of ELP1 exon 20 splicing in Familial
            Dysautonomia by RNA Polymerase II kinetics and chromatin structure
  JOURNAL   PLoS One 19 (6), e0298965 (2024)
   PUBMED   38829854
  REMARK    GeneRIF: Age-dependent regulation of ELP1 exon 20 splicing in
            Familial Dysautonomia by RNA Polymerase II kinetics and chromatin
            structure.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5913)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 5913)
  AUTHORS   Waszak,S.M., Robinson,G.W., Gudenas,B.L., Smith,K.S., Forget,A.,
            Kojic,M., Garcia-Lopez,J., Hadley,J., Hamilton,K.V., Indersie,E.,
            Buchhalter,I., Kerssemakers,J., Jager,N., Sharma,T., Rausch,T.,
            Kool,M., Sturm,D., Jones,D.T.W., Vasilyeva,A., Tatevossian,R.G.,
            Neale,G., Lombard,B., Loew,D., Nakitandwe,J., Rusch,M.,
            Bowers,D.C., Bendel,A., Partap,S., Chintagumpala,M., Crawford,J.,
            Gottardo,N.G., Smith,A., Dufour,C., Rutkowski,S., Eggen,T.,
            Wesenberg,F., Kjaerheim,K., Feychting,M., Lannering,B., Schuz,J.,
            Johansen,C., Andersen,T.V., Roosli,M., Kuehni,C.E., Grotzer,M.,
            Remke,M., Puget,S., Pajtler,K.W., Milde,T., Witt,O., Ryzhova,M.,
            Korshunov,A., Orr,B.A., Ellison,D.W., Brugieres,L., Lichter,P.,
            Nichols,K.E., Gajjar,A., Wainwright,B.J., Ayrault,O., Korbel,J.O.,
            Northcott,P.A. and Pfister,S.M.
  TITLE     Germline Elongator mutations in Sonic Hedgehog medulloblastoma
  JOURNAL   Nature 580 (7803), 396-401 (2020)
   PUBMED   32296180
  REMARK    GeneRIF: Tumours from patients with ELP1-associated medulloblastoma
            subgroup Sonic Hedgehog were characterized by a destabilized
            Elongator complex, loss of Elongator-dependent tRNA modifications,
            codon-dependent translational reprogramming
REFERENCE   5  (bases 1 to 5913)
  AUTHORS   Slaugenhaupt,S.A., Blumenfeld,A., Gill,S.P., Leyne,M., Mull,J.,
            Cuajungco,M.P., Liebert,C.B., Chadwick,B., Idelson,M., Reznik,L.,
            Robbins,C., Makalowska,I., Brownstein,M., Krappmann,D.,
            Scheidereit,C., Maayan,C., Axelrod,F.B. and Gusella,J.F.
  TITLE     Tissue-specific expression of a splicing mutation in the IKBKAP
            gene causes familial dysautonomia
  JOURNAL   Am J Hum Genet 68 (3), 598-605 (2001)
   PUBMED   11179008
REFERENCE   6  (bases 1 to 5913)
  AUTHORS   Ninomiya-Tsuji,J., Kishimoto,K., Hiyama,A., Inoue,J., Cao,Z. and
            Matsumoto,K.
  TITLE     The kinase TAK1 can activate the NIK-I kappaB as well as the MAP
            kinase cascade in the IL-1 signalling pathway
  JOURNAL   Nature 398 (6724), 252-256 (1999)
   PUBMED   10094049
REFERENCE   7  (bases 1 to 5913)
  AUTHORS   Cohen,L., Henzel,W.J. and Baeuerle,P.A.
  TITLE     IKAP is a scaffold protein of the IkappaB kinase complex
  JOURNAL   Nature 395 (6699), 292-296 (1998)
   PUBMED   9751059
REFERENCE   8  (bases 1 to 5913)
  AUTHORS   Blumenfeld,A., Slaugenhaupt,S.A., Axelrod,F.B., Lucente,D.E.,
            Maayan,C., Liebert,C.B., Ozelius,L.J., Trofatter,J.A., Haines,J.L.,
            Breakefield,X.O. et al.
  TITLE     Localization of the gene for familial dysautonomia on chromosome 9
            and definition of DNA markers for genetic diagnosis
  JOURNAL   Nat Genet 4 (2), 160-164 (1993)
   PUBMED   8102296
REFERENCE   9  (bases 1 to 5913)
  AUTHORS   Parisi,M.A.
  TITLE     Hirschsprung Disease Overview - RETIRED CHAPTER, FOR HISTORICAL
            REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301612
REFERENCE   10 (bases 1 to 5913)
  AUTHORS   Bar-Aluma,B.E.
  TITLE     Familial Dysautonomia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301359
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK289962.1, BC033094.1,
            AF153419.2, BF968300.1 and AL359692.9.
            
            On Nov 22, 2018 this sequence version replaced NM_003640.4.
            
            Summary: The protein encoded by this gene is a scaffold protein and
            a regulator for three different kinases involved in proinflammatory
            signaling. The encoded protein can bind NF-kappa-B-inducing kinase
            and I-kappa-B kinases through separate domains and assemble them
            into an active kinase complex. Mutations in this gene have been
            associated with familial dysautonomia. Alternative splicing results
            in multiple transcript variants encoding different isoforms.
            [provided by RefSeq, Jan 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK289962.1, AF153419.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374647.10/ ENSP00000363779.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3384              AK289962.1         1-3384
            3385-5257           BC033094.1         3155-5027
            5258-5573           AF153419.2         5257-5572
            5574-5646           BF968300.1         543-615
            5647-5665           BF968300.1         617-635
            5666-5910           AF153419.2         5665-5909
            5911-5913           AL359692.9         39347-39349         c
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q31.3"
     gene            1..5913
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /note="elongator acetyltransferase complex subunit 1"
                     /db_xref="GeneID:8518"
                     /db_xref="HGNC:HGNC:5959"
                     /db_xref="MIM:603722"
     exon            1..261
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            262..466
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             317..4315
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ikappaB kinase complex-associated protein; inhibitor of
                     kappa light polypeptide gene enhancer in B-cells, kinase
                     complex-associated protein; IKK complex-associated
                     protein; elongator complex protein 1"
                     /codon_start=1
                     /product="elongator complex protein 1 isoform 1"
                     /protein_id="NP_003631.2"
                     /db_xref="CCDS:CCDS6773.1"
                     /db_xref="GeneID:8518"
                     /db_xref="HGNC:HGNC:5959"
                     /db_xref="MIM:603722"
                     /translation="MRNLKLFRTLEFRDIQGPGNPQCFSLRTEQGTVLIGSEHGLIEV
                     DPVSREVKNEVSLVAEGFLPEDGSGRIVGVQDLLDQESVCVATASGDVILCSLSTQQL
                     ECVGSVASGISVMSWSPDQELVLLATGQQTLIMMTKDFEPILEQQIHQDDFGESKFIT
                     VGWGRKETQFHGSEGRQAAFQMQMHESALPWDDHRPQVTWRGDGQFFAVSVVCPETGA
                     RKVRVWNREFALQSTSEPVAGLGPALAWKPSGSLIASTQDKPNQQDIVFFEKNGLLHG
                     HFTLPFLKDEVKVNDLLWNADSSVLAVWLEDLQREESSIPKTCVQLWTVGNYHWYLKQ
                     SLSFSTCGKSKIVSLMWDPVTPYRLHVLCQGWHYLAYDWHWTTDRSVGDNSSDLSNVA
                     VIDGNRVLVTVFRQTVVPPPMCTYQLLFPHPVNQVTFLAHPQKSNDLAVLDASNQISV
                     YKCGDCPSADPTVKLGAVGGSGFKVCLRTPHLEKRYKIQFENNEDQDVNPLKLGLLTW
                     IEEDVFLAVSHSEFSPRSVIHHLTAASSEMDEEHGQLNVSSSAAVDGVIISLCCNSKT
                     KSVVLQLADGQIFKYLWESPSLAIKPWKNSGGFPVRFPYPCTQTELAMIGEEECVLGL
                     TDRCRFFINDIEVASNITSFAVYDEFLLLTTHSHTCQCFCLRDASFKTLQAGLSSNHV
                     SHGEVLRKVERGSRIVTVVPQDTKLVLQMPRGNLEVVHHRALVLAQIRKWLDKLMFKE
                     AFECMRKLRINLNLIYDHNPKVFLGNVETFIKQIDSVNHINLFFTELKEEDVTKTMYP
                     APVTSSVYLSRDPDGNKIDLVCDAMRAVMESINPHKYCLSILTSHVKKTTPELEIVLQ
                     KVHELQGNAPSDPDAVSAEEALKYLLHLVDVNELYDHSLGTYDFDLVLMVAEKSQKDP
                     KEYLPFLNTLKKMETNYQRFTIDKYLKRYEKAIGHLSKCGPEYFPECLNLIKDKNLYN
                     EALKLYSPSSQQYQDISIAYGEHLMQEHMYEPAGLMFARCGAHEKALSAFLTCGNWKQ
                     ALCVAAQLNFTKDQLVGLGRTLAGKLVEQRKHIDAAMVLEECAQDYEEAVLLLLEGAA
                     WEEALRLVYKYNRLDIIETNVKPSILEAQKNYMAFLDSQTATFSRHKKRLLVVRELKE
                     QAQQAGLDDEVPHGQESDLFSETSSVVSGSEMSGKYSHSNSRISARSSKNRRKAERKK
                     HSLKEGSPLEDLALLEALSEVVQNTENLKDEVYHILKVLFLFEFDEQGRELQKAFEDT
                     LQLMERSLPEIWTLTYQQNSATPVLGPNSTANSIMASYQQQKTSVPVLDAELFIPPKI
                     NRRTQWKLSLLD"
     exon            467..619
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            620..701
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            702..782
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            783..868
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            869..965
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1056
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1180
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1274
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1505
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1676
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1776
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1959
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1960..2066
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2170
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..2224
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2225..2330
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2331..2446
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2520
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2521..2599
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2600..2679
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2680..2817
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2818..2903
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2904..3052
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3053..3176
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3177..3274
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3275..3476
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3477..3538
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3539..3601
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3602..3662
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3663..3776
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3777..3888
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3889..4016
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            4017..4171
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            4172..4247
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     exon            4248..5913
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4772..4777
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /note="hexamer: AATAAA"
     polyA_site      4798
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /note="major polyA site"
     regulatory      5233..5238
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /note="hexamer: ATTAAA"
     polyA_site      5262
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /note="major polyA site"
     regulatory      5893..5898
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
                     /note="hexamer: AGTAAA"
     polyA_site      5913
                     /gene="ELP1"
                     /gene_synonym="DYS; FD; IKAP; IKBKAP; IKI3; TOT1"
ORIGIN      
        1 gcactcccag tgctgcggct gcctagttga cgcacccatt gagtcgctgg cttctttgca
       61 gcgcttcagc gttttcccct ggagggcgcc tccatccttg gaggcctagt gccgtcggag
      121 agagagcggg agccgcggac agagacgcgt gcgcaattcg gagccgactc tgggtgcgga
      181 ctgtgggagc tgactctggg tagccggctg cgcgtggctg gggaggcgag gccggacgca
      241 cctctgtttg ggggtcctca gagattaatg attcatcaag ggatagttgt acttgtctcg
      301 tgggaatcac ttcatcatgc gaaatctgaa attatttcgg accctggagt tcagggatat
      361 tcaaggtcca gggaatcctc agtgcttctc tctccgaact gaacagggga cggtgctcat
      421 tggttcagaa catggcctga tagaagtaga ccctgtctca agagaagtga aaaatgaagt
      481 ttctttggtg gcagaaggct ttctcccaga ggatggaagt ggccgcattg ttggtgttca
      541 ggacttgctg gatcaggagt ctgtgtgtgt ggccacagcc tctggagacg tcatactctg
      601 cagtctcagc acacaacagc tggagtgtgt tgggagtgta gccagtggta tctctgttat
      661 gagttggagt cctgaccaag agctggtgct tcttgccaca ggtcaacaga ccctgattat
      721 gatgacaaaa gattttgagc caatcctgga gcagcagatc catcaggatg attttggtga
      781 aagcaagttt atcactgttg gatggggtag gaaggagaca cagttccatg gatcagaagg
      841 cagacaagca gcttttcaga tgcaaatgca tgagtctgct ttgccctggg atgaccatag
      901 accacaagtt acctggcggg gggatggaca gttttttgct gtgagtgttg tttgcccaga
      961 aacaggggct cggaaggtca gagtgtggaa ccgagagttt gctttgcagt caaccagtga
     1021 gcctgtggca ggactgggac cagccctggc ttggaaaccc tcaggcagtt tgattgcatc
     1081 tacacaagat aaacccaacc agcaggatat tgtgtttttt gagaaaaatg gactccttca
     1141 tggacacttt acacttccct tccttaaaga tgaggttaag gtaaatgact tgctctggaa
     1201 tgcagattcc tctgtgcttg cagtctggct ggaagacctt cagagagaag aaagctccat
     1261 tccgaaaacc tgtgttcagc tctggactgt tggaaactat cactggtatc tcaagcaaag
     1321 tttatccttc agcacctgtg ggaagagcaa gattgtgtct ctgatgtggg accctgtgac
     1381 cccataccgg ctgcatgttc tctgtcaggg ctggcattac ctcgcctatg attggcactg
     1441 gacgactgac cggagcgtgg gagataattc aagtgacttg tccaatgtgg ctgtcattga
     1501 tggaaacagg gtgttggtga cagtcttccg gcagactgtg gttccgcctc ccatgtgcac
     1561 ctaccaactg ctgttcccac accctgtgaa tcaagtcaca ttcttagcac accctcaaaa
     1621 gagtaatgac cttgctgttc tagatgccag taaccagatt tctgtttata aatgtggtga
     1681 ttgtccaagt gctgacccta cagtgaaact gggagctgtg ggtggaagtg gatttaaagt
     1741 ttgccttaga actcctcatt tggaaaagag atacaaaatc cagtttgaga ataatgaaga
     1801 tcaagatgta aacccgctga aactaggcct tctcacttgg attgaagaag acgtcttcct
     1861 ggctgtaagc cacagtgagt tcagcccccg gtctgtcatt caccatttga ctgcagcttc
     1921 ttctgagatg gatgaagagc atggacagct caatgtcagt tcatctgcag cggtggatgg
     1981 ggtcataatc agtctatgtt gcaattccaa gaccaagtca gtagtattac agctggctga
     2041 tggccagata tttaagtacc tttgggagtc accttctctg gctattaaac catggaagaa
     2101 ctctggtgga tttcctgttc ggtttcctta tccatgcacc cagaccgaat tggccatgat
     2161 tggagaagag gaatgtgtcc ttggtctgac tgacaggtgt cgctttttca tcaatgacat
     2221 tgaggttgcg tcaaatatca cgtcatttgc agtatatgat gagtttttat tgttgacaac
     2281 ccattcccat acctgccagt gtttttgcct gagggatgct tcatttaaaa cattacaggc
     2341 cggcctgagc agcaatcatg tgtcccatgg ggaagttctg cggaaagtgg agaggggttc
     2401 acggattgtc actgttgtgc cccaggacac aaagcttgta ttacagatgc caaggggaaa
     2461 cttagaagtt gttcatcatc gagccctggt tttagctcag attcggaagt ggttggacaa
     2521 acttatgttt aaagaggcat ttgaatgcat gagaaagctg agaatcaatc tcaatctgat
     2581 ttatgatcat aaccctaagg tgtttcttgg aaatgtggaa accttcatta aacagataga
     2641 ttctgtgaat catattaact tgttttttac agaattgaaa gaagaagatg tcacgaagac
     2701 catgtaccct gcaccagtta ccagcagtgt ctacctgtcc agggatcctg acgggaataa
     2761 aatagacctt gtctgcgatg ctatgagagc agtcatggag agcataaatc ctcataaata
     2821 ctgcctatcc atacttacat ctcatgtaaa gaagacaacc ccagaactgg aaattgtact
     2881 gcaaaaagta cacgagcttc aaggaaatgc tccctctgat cctgatgctg tgagtgctga
     2941 agaggccttg aaatatttgc tgcatctggt agatgttaat gaattatatg atcattctct
     3001 tggcacctat gactttgatt tggtcctcat ggtagctgag aagtcacaga aggatcccaa
     3061 agaatatctt ccatttctta atacacttaa gaaaatggaa actaattatc agcggtttac
     3121 tatagacaaa tacttgaaac gatatgaaaa agccattggc cacctcagca aatgtggacc
     3181 tgagtacttc ccagaatgct taaacttgat aaaagataaa aacttgtata acgaagctct
     3241 gaagttatat tcaccaagct cacaacagta ccaggatatc agcattgctt atggggagca
     3301 cctgatgcag gagcacatgt atgagccagc ggggctcatg tttgcccgtt gcggtgccca
     3361 cgagaaagct ctctcagcct ttctgacatg tggcaactgg aagcaagccc tctgtgtggc
     3421 agcccagctt aactttacca aagaccagct ggtgggcctc ggcagaactc tggcaggaaa
     3481 gctggttgag cagaggaagc acattgatgc ggccatggtt ttggaagagt gtgcccagga
     3541 ttatgaagaa gctgtgctct tgctgttaga aggagctgcc tgggaagaag ctttgaggct
     3601 ggtatacaaa tataacagac tggatattat agaaaccaac gtaaagcctt ccattttaga
     3661 agcccagaaa aattatatgg catttctgga ctctcagaca gccacattca gtcgccacaa
     3721 gaaacgttta ttggtagttc gagagctcaa ggagcaagcc cagcaggcag gtctggatga
     3781 tgaggtaccc cacgggcaag agtcagacct cttctctgaa actagcagtg tcgtgagtgg
     3841 cagtgagatg agtggcaaat actcccatag taactccagg atatcagcga gatcatccaa
     3901 gaatcgccga aaagcggagc ggaagaagca cagcctcaaa gaaggcagtc cgctggagga
     3961 cctggccctc ctggaggcac tgagtgaagt ggtgcagaac actgaaaacc tgaaagatga
     4021 agtataccat attttaaagg tactctttct ctttgagttt gatgaacaag gaagggaatt
     4081 acagaaggcc tttgaagata cgctgcagtt gatggaaagg tcacttccag aaatttggac
     4141 tcttacttac cagcagaatt cagctacccc ggttctaggt cccaattcta ctgcaaatag
     4201 tatcatggca tcttatcagc aacagaagac ttcggttcct gttcttgatg ctgagctttt
     4261 tataccacca aagatcaaca gaagaaccca gtggaagctg agcctgctag actgagtgac
     4321 tgcagttagg agggatccga cagagaagac catttccact cattcctgtt gtcctaccac
     4381 cccttgctct ttgagggctg gctattgaga actggaaaga gtaaaatgat aacttacctt
     4441 agcattgcca agaacttcag cagacaacaa gcaattctat ttattttatg ttgtgtatac
     4501 atcttgatca ttagcaagac attaagcttt aaccattatg gcaccatttt gtgagaatga
     4561 ttgttctttc acttgggctg tttgagagca taattatggt aatcatgaga ttaatgtttc
     4621 atgatttcta cctccaaagt gtgaagacaa gtaaaacaat gtttctaaat tgtcttattt
     4681 tgttggcgga gaagattaca atggctatta gtgctacatt tggtcaaatg taatcactta
     4741 aatagcttct tgtcacctta aactaaagca gaataaaaag tatcctttga aattataagc
     4801 cctcctttgc tgacagctat tattttgtaa catcttacca ggtcatgtgc tttcagttat
     4861 aactgggctg agcctcctat aattacaatg tctataggga ctgttttact gcctgtgtat
     4921 tttctgctag agagttagca atgttagagc tagaacagat tagaatttct aaacagtatc
     4981 atgcacagtt ggtgtgagtg atcagtgtgc attgtatggc atgcatggtt gtgaattatt
     5041 ctctgttctc caaatactgt ttctttaact cagatatttt tgttagtgtc taggccactt
     5101 catttatttt tcgtcatggt actttactga cttctcttta ttcaattctc cacgccctca
     5161 ccaaaaaaaa ctgtctcaaa atgagaatat tttattttca tggtgagtct agaaaacgcc
     5221 cacttcattc tgattaaaaa ttcttccatg ttttaaatat cagaaccaga cctttcttac
     5281 tgtgtatctt agcccatttg tgtctctata acaacaacca gctttcaaag gaactaatag
     5341 agtgaaaact cactcattac cacgaggatg gcacaagcga ttcacgtagg atctgcccct
     5401 gtgaccaaaa cacctcccat tgggccccac ttccaacact ggtgatcaca tttcaacatg
     5461 aggtttaggg aaacaaatgc ctaaactaca gcactgtaca taaactaaca ggaaatgctg
     5521 cttttgatcc tcaaagaagt gatatagcca aaattgtaat ttaagaagcc tttcccagta
     5581 tagcaagatg ttaactatag aatcaatcta ggagtattca ctgtaaaatt caacttttct
     5641 gtatgtttga acattttcac aatctcatag gagtttttaa aaagaagaga aagaagatat
     5701 actttgcttt ggagaaatct actttttgac ttacatgggt ttgctgtaat taagtgccca
     5761 atattgaaag gctgcaagta ctttgtaatc actctttggc atgggtaaat aagcatggta
     5821 acttatattg aaatatagtg ctcttgcttt ggataactgt aaagggaccc atgctgatag
     5881 actggaaata gaagtaaatg tgtttattga taa
//
