LOCUS       NM_007108                974 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens elongin B (ELOB), transcript variant 1, mRNA.
ACCESSION   NM_007108
VERSION     NM_007108.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Li,G., Lin,X., Wang,X., Cai,L., Liu,J., Zhu,Y. and Fu,Z.
  TITLE     Enhancing radiosensitivity in triple-negative breast cancer through
            targeting ELOB
  JOURNAL   Breast Cancer 31 (3), 426-439 (2024)
   PUBMED   38472737
  REMARK    GeneRIF: Enhancing radiosensitivity in triple-negative breast
            cancer through targeting ELOB.
REFERENCE   2  (bases 1 to 974)
  AUTHORS   Sui,X.Y., Ma,X.Y., Hou,Y., Cao,S.W., Wang,Z.Q., Jia,L.J., Fan,L.,
            Shao,Z.M. and Zhang,W.J.
  TITLE     Elongin B promotes breast cancer progression by ubiquitinating
            tumor suppressor p14/ARF
  JOURNAL   Cell Biol Toxicol 40 (1), 24 (2024)
   PUBMED   38653919
  REMARK    GeneRIF: Elongin B promotes breast cancer progression by
            ubiquitinating tumor suppressor p14/ARF.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 974)
  AUTHORS   Xu,P., Liu,Y., Liu,C., Guey,B., Li,L., Melenec,P., Ricci,J. and
            Ablasser,A.
  TITLE     The CRL5-SPSB3 ubiquitin ligase targets nuclear cGAS for
            degradation
  JOURNAL   Nature 627 (8005), 873-879 (2024)
   PUBMED   38418882
REFERENCE   4  (bases 1 to 974)
  AUTHORS   Ramachandran,S., Makukhin,N., Haubrich,K., Nagala,M., Forrester,B.,
            Lynch,D.M., Casement,R., Testa,A., Bruno,E., Gitto,R. and Ciulli,A.
  TITLE     Structure-based design of a phosphotyrosine-masked covalent ligand
            targeting the E3 ligase SOCS2
  JOURNAL   Nat Commun 14 (1), 6345 (2023)
   PUBMED   37816714
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 974)
  AUTHORS   Linossi,E.M., Li,K., Veggiani,G., Tan,C., Dehkhoda,F., Hockings,C.,
            Calleja,D.J., Keating,N., Feltham,R., Brooks,A.J., Li,S.S.,
            Sidhu,S.S., Babon,J.J., Kershaw,N.J. and Nicholson,S.E.
  TITLE     Discovery of an exosite on the SOCS2-SH2 domain that enhances SH2
            binding to phosphorylated ligands
  JOURNAL   Nat Commun 12 (1), 7032 (2021)
   PUBMED   34857742
  REMARK    Erratum:[Nat Commun. 2023 Dec 1;14(1):7951. doi:
            10.1038/s41467-023-42706-4.. PMID: 38040684]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 974)
  AUTHORS   Pause,A., Lee,S., Worrell,R.A., Chen,D.Y., Burgess,W.H.,
            Linehan,W.M. and Klausner,R.D.
  TITLE     The von Hippel-Lindau tumor-suppressor gene product forms a stable
            complex with human CUL-2, a member of the Cdc53 family of proteins
  JOURNAL   Proc Natl Acad Sci U S A 94 (6), 2156-2161 (1997)
   PUBMED   9122164
REFERENCE   7  (bases 1 to 974)
  AUTHORS   Kibel,A., Iliopoulos,O., DeCaprio,J.A. and Kaelin,W.G. Jr.
  TITLE     Binding of the von Hippel-Lindau tumor suppressor protein to
            Elongin B and C
  JOURNAL   Science 269 (5229), 1444-1446 (1995)
   PUBMED   7660130
REFERENCE   8  (bases 1 to 974)
  AUTHORS   Aso,T., Lane,W.S., Conaway,J.W. and Conaway,R.C.
  TITLE     Elongin (SIII): a multisubunit regulator of elongation by RNA
            polymerase II
  JOURNAL   Science 269 (5229), 1439-1443 (1995)
   PUBMED   7660129
REFERENCE   9  (bases 1 to 974)
  AUTHORS   Duan,D.R., Pause,A., Burgess,W.H., Aso,T., Chen,D.Y., Garrett,K.P.,
            Conaway,R.C., Conaway,J.W., Linehan,W.M. and Klausner,R.D.
  TITLE     Inhibition of transcription elongation by the VHL tumor suppressor
            protein
  JOURNAL   Science 269 (5229), 1402-1406 (1995)
   PUBMED   7660122
REFERENCE   10 (bases 1 to 974)
  AUTHORS   Garrett,K.P., Aso,T., Bradsher,J.N., Foundling,S.I., Lane,W.S.,
            Conaway,R.C. and Conaway,J.W.
  TITLE     Positive regulation of general transcription factor SIII by a
            tailed ubiquitin homolog
  JOURNAL   Proc Natl Acad Sci U S A 92 (16), 7172-7176 (1995)
   PUBMED   7638163
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC092117.4, BC013306.2 and
            DT217333.1.
            
            On Jun 2, 2019 this sequence version replaced NM_007108.3.
            
            Summary: This gene encodes the protein elongin B, which is a
            subunit of the transcription factor B (SIII) complex. The SIII
            complex is composed of elongins A/A2, B and C. It activates
            elongation by RNA polymerase II by suppressing transient pausing of
            the polymerase at many sites within transcription units. Elongin A
            functions as the transcriptionally active component of the SIII
            complex, whereas elongins B and C are regulatory subunits. Elongin
            A2 is specifically expressed in the testis, and capable of forming
            a stable complex with elongins B and C. The von Hippel-Lindau tumor
            suppressor protein binds to elongins B and C, and thereby inhibits
            transcription elongation. Two alternatively spliced transcript
            variants encoding different isoforms have been described for this
            gene. Pseudogenes have been identified on chromosomes 11 and 13.
            [provided by RefSeq, Aug 2008].
            
            Transcript Variant: This variant (1) encodes the predominant
            isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189652.53580.1, BC013306.2
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000409906.9/ ENSP00000386652.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AC092117.4         168322-168351       c
            31-399              BC013306.2         1-369
            400-671             DT217333.1         392-663
            672-974             BC013306.2         641-943
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..974
                     /gene="ELOB"
                     /gene_synonym="SIII; TCEB2"
                     /note="elongin B"
                     /db_xref="GeneID:6923"
                     /db_xref="HGNC:HGNC:11619"
                     /db_xref="MIM:600787"
     exon            1..44
                     /gene="ELOB"
                     /gene_synonym="SIII; TCEB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..398
                     /gene="ELOB"
                     /gene_synonym="SIII; TCEB2"
                     /note="isoform a is encoded by transcript variant 1; SIII
                     p18; transcription elongation factor B polypeptide 2;
                     epididymis secretory sperm binding protein; transcription
                     elongation factor B (SIII), polypeptide 2 (18kDa, elongin
                     B); RNA polymerase II transcription factor SIII p18
                     subunit; elongin, 18-kD subunit; elongin 18 kDa subunit;
                     RNA polymerase II transcription factor SIII subunit B;
                     transcription elongation factor B subunit 2"
                     /codon_start=1
                     /product="elongin-B isoform a"
                     /protein_id="NP_009039.1"
                     /db_xref="CCDS:CCDS45387.1"
                     /db_xref="GeneID:6923"
                     /db_xref="HGNC:HGNC:11619"
                     /db_xref="MIM:600787"
                     /translation="MDVFLMIRRHKTTIFTDAKESSTVFELKRIVEGILKRPPDEQRL
                     YKDDQLLDDGKTLGECGFTSQTARPQAPATVGLAFRADDTFEALCIEPFSSPPELPDV
                     MKPQDSGSSANEQAVQ"
     exon            45..179
                     /gene="ELOB"
                     /gene_synonym="SIII; TCEB2"
                     /inference="alignment:Splign:2.1.0"
     exon            180..285
                     /gene="ELOB"
                     /gene_synonym="SIII; TCEB2"
                     /inference="alignment:Splign:2.1.0"
     exon            286..974
                     /gene="ELOB"
                     /gene_synonym="SIII; TCEB2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      425..430
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ELOB"
                     /gene_synonym="SIII; TCEB2"
                     /note="hexamer: AATAAA"
     polyA_site      447
                     /gene="ELOB"
                     /gene_synonym="SIII; TCEB2"
                     /note="major polyA site"
     regulatory      954..959
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ELOB"
                     /gene_synonym="SIII; TCEB2"
                     /note="hexamer: AATAAA"
     polyA_site      974
                     /gene="ELOB"
                     /gene_synonym="SIII; TCEB2"
ORIGIN      
        1 gcggctgcgc cggcgcgtcg aggggagagg cagcagccgc gatggacgtg ttcctcatga
       61 tccggcgcca caagaccacc atcttcacgg acgccaagga gtccagcacg gtgttcgaac
      121 tgaagcgcat cgtcgagggc atcctcaagc ggcctcctga cgagcagcgg ctgtacaagg
      181 atgaccaact cttggatgat ggcaagacac tgggcgagtg tggcttcacc agtcaaacag
      241 cacggccaca ggccccagcc acagtggggc tggccttccg ggcagatgac acctttgagg
      301 ccctgtgcat cgagccgttt tccagcccgc cagagctgcc cgatgtgatg aagccccagg
      361 actcgggaag cagtgccaat gaacaagccg tgcagtgagg acccccaaga ggcccatttc
      421 ccccaataaa agagatttgg gagtctgcct ggttgctgcc tctttttccc gcccctccct
      481 gggatgggtc ccactccctg tgggctcctt ttggggcttg tgcttggcag ttcctgtgct
      541 gtcctgtctc ccagatcctg agaccctggc tgagaacttg gcccagcctg ctgcttaaag
      601 gcaccatggg gacctgggtt gccctgagac ccaagccatt gttagcagct agccagccac
      661 accaaccacg ccagggggag gaaagggaag gactgggaga gacacaaaga ccagagccag
      721 cctcagggac aagagattcc agttttaggc ctttctcctt ctaagtgccc cccaccccca
      781 tagcctgcat gtccactccc agacgatggc caagagcaga aacacaagct ggagccagtg
      841 tcctggtttg acagcatgtt caacgaggga accccaagac ggacccacac aggtccaccc
      901 acgctggggg ctgtaatcac ggagggaagt ggctgccccc ttaacacacc tttaataaac
      961 agtctacaga ccca
//
