LOCUS       NM_015904               5741 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens eukaryotic translation initiation factor 5B (EIF5B),
            mRNA.
ACCESSION   NM_015904
VERSION     NM_015904.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Lapointe,C.P., Grosely,R., Sokabe,M., Alvarado,C., Wang,J.,
            Montabana,E., Villa,N., Shin,B.S., Dever,T.E., Fraser,C.S.,
            Fernandez,I.S. and Puglisi,J.D.
  TITLE     eIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit
            joining
  JOURNAL   Nature 607 (7917), 185-190 (2022)
   PUBMED   35732735
  REMARK    GeneRIF: eIF5B and eIF1A reorient initiator tRNA to allow ribosomal
            subunit joining.
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Paul,E.E., Lin,K.Y., Gamble,N., Tsai,A.W., Swan,S.H.K., Yang,Y.,
            Doran,M. and Marintchev,A.
  TITLE     Dynamic interaction network involving the conserved intrinsically
            disordered regions in human eIF5
  JOURNAL   Biophys Chem 281, 106740 (2022)
   PUBMED   34923394
  REMARK    GeneRIF: Dynamic interaction network involving the conserved
            intrinsically disordered regions in human eIF5.
REFERENCE   3  (bases 1 to 5741)
  AUTHORS   Li,Q., Xiao,M., Shi,Y., Hu,J., Bi,T., Wang,C., Yan,L. and Li,X.
  TITLE     eIF5B regulates the expression of PD-L1 in prostate cancer cells by
            interacting with Wig1
  JOURNAL   BMC Cancer 21 (1), 1022 (2021)
   PUBMED   34525951
  REMARK    GeneRIF: eIF5B regulates the expression of PD-L1 in prostate cancer
            cells by interacting with Wig1.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5741)
  AUTHORS   Bressler,K.R., Ross,J.A., Ilnytskyy,S., Vanden Dungen,K.,
            Taylor,K., Patel,K., Zovoilis,A., Kovalchuk,I. and Thakor,N.
  TITLE     Depletion of eukaryotic initiation factor 5B (eIF5B) reprograms the
            cellular transcriptome and leads to activation of endoplasmic
            reticulum (ER) stress and c-Jun N-terminal kinase (JNK)
  JOURNAL   Cell Stress Chaperones 26 (1), 253-264 (2021)
   PUBMED   33123915
  REMARK    GeneRIF: Depletion of eukaryotic initiation factor 5B (eIF5B)
            reprograms the cellular transcriptome and leads to activation of
            endoplasmic reticulum (ER) stress and c-Jun N-terminal kinase
            (JNK).
REFERENCE   5  (bases 1 to 5741)
  AUTHORS   Suresh,S., Chen,B., Zhu,J., Golden,R.J., Lu,C., Evers,B.M.,
            Novaresi,N., Smith,B., Zhan,X., Schmid,V., Jun,S., Karacz,C.M.,
            Peyton,M., Zhong,L., Wen,Z., Sathe,A.A., Xing,C., Behrens,C.,
            Wistuba,I.I., Xiao,G., Xie,Y., Fu,Y.X., Minna,J.D., Mendell,J.T.
            and O'Donnell,K.A.
  TITLE     eIF5B drives integrated stress response-dependent translation of
            PD-L1 in lung cancer
  JOURNAL   Nat Cancer 1 (5), 533-545 (2020)
   PUBMED   32984844
  REMARK    GeneRIF: eIF5B drives integrated stress response-dependent
            translation of PD-L1 in lung cancer.
REFERENCE   6  (bases 1 to 5741)
  AUTHORS   Bohnsack,M.T., Regener,K., Schwappach,B., Saffrich,R.,
            Paraskeva,E., Hartmann,E. and Gorlich,D.
  TITLE     Exp5 exports eEF1A via tRNA from nuclei and synergizes with other
            transport pathways to confine translation to the cytoplasm
  JOURNAL   EMBO J 21 (22), 6205-6215 (2002)
   PUBMED   12426392
REFERENCE   7  (bases 1 to 5741)
  AUTHORS   Sorensen,H.P., Hedegaard,J., Sperling-Petersen,H.U. and
            Mortensen,K.K.
  TITLE     Remarkable conservation of translation initiation factors:
            IF1/eIF1A and IF2/eIF5B are universally distributed phylogenetic
            markers
  JOURNAL   IUBMB Life 51 (5), 321-327 (2001)
   PUBMED   11699879
REFERENCE   8  (bases 1 to 5741)
  AUTHORS   Pestova,T.V., Lomakin,I.B., Lee,J.H., Choi,S.K., Dever,T.E. and
            Hellen,C.U.
  TITLE     The joining of ribosomal subunits in eukaryotes requires eIF5B
  JOURNAL   Nature 403 (6767), 332-335 (2000)
   PUBMED   10659855
REFERENCE   9  (bases 1 to 5741)
  AUTHORS   Wilson,S.A., Sieiro-Vazquez,C., Edwards,N.J., Iourin,O.,
            Byles,E.D., Kotsopoulou,E., Adamson,C.S., Kingsman,S.M.,
            Kingsman,A.J. and Martin-Rendon,E.
  TITLE     Cloning and characterization of hIF2, a human homologue of
            bacterial translation initiation factor 2, and its interaction with
            HIV-1 matrix
  JOURNAL   Biochem J 342 (Pt 1) (Pt 1), 97-103 (1999)
   PUBMED   10432305
REFERENCE   10 (bases 1 to 5741)
  AUTHORS   Lee,J.H., Choi,S.K., Roll-Mecak,A., Burley,S.K. and Dever,T.E.
  TITLE     Universal conservation in translation initiation revealed by human
            and archaeal homologs of bacterial translation initiation factor
            IF2
  JOURNAL   Proc Natl Acad Sci U S A 96 (8), 4342-4347 (1999)
   PUBMED   10200264
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX485839.1, AB018284.1,
            BC032639.1 and AC018690.5.
            
            On Nov 22, 2018 this sequence version replaced NM_015904.3.
            
            Summary: Accurate initiation of translation in eukaryotes is
            complex and requires many factors, some of which are composed of
            multiple subunits. The process is simpler in prokaryotes which have
            only three initiation factors (IF1, IF2, IF3). Two of these factors
            are conserved in eukaryotes: the homolog of IF1 is eIF1A and the
            homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and
            eIF5B interact on the ribosome along with other initiation factors
            and GTP to position the initiation methionine tRNA on the start
            codon of the mRNA so that translation initiates accurately.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB018284.1, AJ006776.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000289371.11/ ENSP00000289371.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                BX485839.1         19-34
            17-356              AB018284.1         1-340
            357-1730            BC032639.1         297-1670
            1731-4193           AB018284.1         1715-4177
            4194-4680           AC018690.5         29586-30072
            4681-5741           AC018690.5         30073-31133
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q11.2"
     gene            1..5741
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /note="eukaryotic translation initiation factor 5B"
                     /db_xref="GeneID:9669"
                     /db_xref="HGNC:HGNC:30793"
                     /db_xref="MIM:606086"
     exon            1..201
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..3829
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /EC_number="3.6.5.3"
                     /note="translation initiation factor IF2; eIF-5B;
                     translation initiation factor IF-2"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 5B"
                     /protein_id="NP_056988.3"
                     /db_xref="CCDS:CCDS42721.1"
                     /db_xref="GeneID:9669"
                     /db_xref="HGNC:HGNC:30793"
                     /db_xref="MIM:606086"
                     /translation="MGKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKEQEPQKSKGKK
                     KKEKKKQDFDEDDILKELEELSLEAQGIKADRETVAVKPTENNEEEFTSKDKKKKGQK
                     GKKQSFDDNDSEELEDKDSKSKKTAKPKVEMYSGSDDDDDFNKLPKKAKGKAQKSNKK
                     WDGSEEDEDNSKKIKERSRINSSGESGDESDEFLQSRKGQKKNQKNKPGPNIESGNED
                     DDASFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQKESQRKF
                     EEETVKSKVTVDTGVIPASEEKAETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKK
                     GPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQ
                     KEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKK
                     RKKIPQQLESKEVSESMELCAAVEVMEQGVPEKEETPPPVEPEEEEDTEDAGLDDWEA
                     MASDEETEKVEGNKVHIEVKENPEEEEEEEEEEEEDEESEEEEEEEGESEGSEGDEED
                     EKVSDEKDSGKTLDKKPSKEMSSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNV
                     NTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQT
                     KMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIES
                     INLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEF
                     AQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEEL
                     RAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELR
                     VKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLN
                     AIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQ
                     YAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIA
                     VFPCKIKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHK
                     QVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQK
                     SDWQLIVELKKVFEII"
     exon            202..327
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            328..412
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            413..1085
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1303
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1454
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1553
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1643
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1644..1718
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1719..2008
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2116
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2117..2227
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2228..2295
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2296..2437
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2438..2569
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2570..2752
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2753..2914
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2915..3046
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3047..3178
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3179..3255
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3256..3420
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3421..3559
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3560..3721
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3722..5741
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4163..4168
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /note="hexamer: ATTAAA"
     polyA_site      4195
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /note="major polyA site"
     regulatory      4461..4466
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /note="hexamer: AAGAAA"
     polyA_site      4484
                     /gene="EIF5B"
                     /gene_synonym="IF2"
     regulatory      5716..5721
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF5B"
                     /gene_synonym="IF2"
                     /note="hexamer: AATAAA"
     polyA_site      5741
                     /gene="EIF5B"
                     /gene_synonym="IF2"
ORIGIN      
        1 acaccatatg tgtcctgttc cagtgcgcgg gtctgtggag agccgggtgc gagcggcggc
       61 agcacgaggg gaaaagagct gagcggagac caaagtcagc cgggagacag tgggtctgtg
      121 agagaccgaa tagaggggct ggggccacga gcgccattga caagcaatgg ggaagaaaca
      181 gaaaaacaag agcgaagaca gcaccaagga tgacattgat cttgatgcct tggctgcaga
      241 aatagaagga gctggtgctg ccaaagaaca ggagcctcaa aagtcaaaag ggaaaaagaa
      301 aaaagagaaa aaaaagcagg actttgatga agatgatatc ctgaaagaac tggaagaatt
      361 gtctttggaa gctcaaggca tcaaagctga cagagaaact gttgcagtga agccaacaga
      421 aaacaatgaa gaggaattca cctcaaaaga taaaaaaaag aaaggacaga agggcaaaaa
      481 acagagtttt gatgataatg atagcgaaga attggaagat aaagattcaa aatcaaaaaa
      541 gactgcaaaa ccgaaagtgg aaatgtactc tgggagtgat gatgatgatg attttaacaa
      601 acttcctaaa aaagctaaag ggaaagctca aaaatcaaat aagaagtggg atgggtcaga
      661 ggaggatgag gataacagta aaaaaattaa agagcgttca agaataaatt cttctggtga
      721 aagtggtgat gaatcagatg aatttttgca atctagaaaa ggacagaaaa aaaatcagaa
      781 aaacaagcca ggtcctaaca tagaaagtgg gaatgaagat gatgacgcct ccttcaaaat
      841 taagacagtg gcccaaaaga aggcagaaaa gaaggagcgc gagagaaaaa agcgagatga
      901 agaaaaagcg aaactgcgga agctgaaaga aaaagaagag ttagaaacag gtaaaaagga
      961 tcagagtaaa caaaaggaat ctcaaaggaa atttgaagaa gaaactgtaa aatccaaagt
     1021 gactgttgat actggagtaa ttcctgcctc tgaagagaaa gcagagactc ccacagctgc
     1081 agaagatgac aatgaaggag acaaaaagaa gaaagataag aagaaaaaga aaggagaaaa
     1141 ggaagaaaaa gagaaagaga agaaaaaagg acctagcaaa gccactgtta aagctatgca
     1201 agaagctctg gctaagctta aagaggaaga agaaagacag aagagagaag aggaagaacg
     1261 tataaaacgg cttgaagaat tagaagccaa gcgtaaagaa gaggaacgat tggaacaaga
     1321 aaaaagagaa aggaaaaagc aaaaagaaaa agaaagaaaa gaacgcttga aaaaagaagg
     1381 gaaactttta actaaatccc agagagaagc cagagccaga gccgaagcta ctcttaaact
     1441 gctacaagct cagggtgttg aagtgccatc aaaagactct ttgccaaaga agaggccaat
     1501 ttatgaagat aaaaagagga aaaaaatacc acagcagcta gaaagtaaag aagtgtctga
     1561 atcaatggaa ttatgtgctg ctgtagaagt tatggaacaa ggagtaccag aaaaggaaga
     1621 gacaccacct cctgttgaac cagaagaaga agaagatact gaggatgctg gattggatga
     1681 ttgggaagct atggccagtg atgaggagac agaaaaagta gaaggaaaca aagttcatat
     1741 agaagtaaaa gaaaaccctg aagaggagga ggaggaggaa gaagaggaag aagaagatga
     1801 agaaagtgaa gaagaggagg aagaggaggg agaaagtgaa ggcagtgaag gtgatgagga
     1861 agatgaaaag gtgtcagatg agaaggattc agggaagaca ttagataaaa agccaagtaa
     1921 agaaatgagc tcagattctg aatatgactc tgatgatgat cggactaaag aagaaagggc
     1981 ttatgacaaa gcaaaacgga ggattgagaa acggcgactt gaacatagta aaaatgtaaa
     2041 caccgaaaag ctaagagccc ctattatctg cgtacttggg catgtggaca cagggaagac
     2101 aaaaattcta gataagctcc gtcacacaca tgtacaagat ggtgaagcag gtggtatcac
     2161 acaacaaatt ggggccacca atgttcctct tgaagctatt aatgaacaga ctaagatgat
     2221 taaaaatttt gatagagaga atgtacggat tccaggaatg ctaattattg atactcctgg
     2281 gcatgaatct ttcagtaatc tgagaaatag aggaagctct ctttgtgaca ttgccatttt
     2341 agttgttgat attatgcatg gtttggagcc ccagacaatt gagtctatca accttctcaa
     2401 atctaaaaaa tgtcccttca ttgttgcact caataagatt gataggttat atgattggaa
     2461 aaagagtcct gactctgatg tggctgctac tttaaagaag cagaaaaaga atacaaaaga
     2521 tgaatttgag gagcgagcaa aggctattat tgtagaattt gcacagcagg gtttgaatgc
     2581 tgctttgttt tatgagaata aagatccccg cacttttgtg tctttggtac ctacctctgc
     2641 acatactggt gatggcatgg gaagtctgat ctaccttctt gtagagttaa ctcagaccat
     2701 gttgagcaag agacttgcac actgtgaaga gctgagagca caggtgatgg aggttaaagc
     2761 tctcccgggg atgggcacca ctatagatgt catcttgatc aatgggcgtt tgaaggaagg
     2821 agatacaatc attgttcctg gagtagaagg gcccattgta actcagattc gaggcctcct
     2881 gttacctcct cctatgaagg aattacgagt gaagaaccag tatgaaaagc ataaagaagt
     2941 agaagcagct cagggggtaa agattcttgg aaaagacctg gagaaaacat tggctggttt
     3001 acccctcctt gtggcttata aagaagatga aatccctgtt cttaaagatg aattgatcca
     3061 tgagttaaag cagacactaa atgctatcaa attagaagaa aaaggagtct atgtccaggc
     3121 atctacactg ggttctttgg aagctctact ggaatttctg aaaacatcag aagtgcccta
     3181 tgcaggaatt aacattggcc cagtgcataa aaaagatgtt atgaaggctt cagtgatgtt
     3241 ggaacatgac cctcagtatg cagtaatttt ggccttcgat gtgagaattg aacgagatgc
     3301 acaagaaatg gctgatagtt taggagttag aatttttagt gcagaaatta tttatcattt
     3361 atttgatgcc tttacaaaat atagacaaga ctacaagaaa cagaaacaag aagaatttaa
     3421 gcacatagca gtatttccct gcaagataaa aatcctccct cagtacattt ttaattctcg
     3481 agatccgata gtgatggggg tgacggtgga agcaggtcag gtgaaacagg ggacacccat
     3541 gtgtgtccca agcaaaaatt ttgttgacat cggaatagta acaagtattg aaataaacca
     3601 taaacaagtg gatgttgcaa aaaaaggaca agaagtttgt gtaaaaatag aacctatccc
     3661 tggtgagtca cccaaaatgt ttggaagaca ttttgaagct acagatattc ttgttagtaa
     3721 gatcagccgg cagtccattg atgcactcaa agactggttc agagatgaaa tgcagaagag
     3781 tgactggcag cttattgtgg agctgaagaa agtatttgaa atcatctaat tttttcacat
     3841 ggagcaggaa ctggagtaaa tgcaatactg tgttgtaata tcccaacaaa aatcagacaa
     3901 aaaatggaac agacgtattt ggacactgat ggacttaagt atggaaggaa gaaaaatagg
     3961 tgtataaaat gttttccatg agaaaccaag aaacttacac tggtttgaca gtggtcagtt
     4021 acatgtcccc acagttccaa tgtgcctgtt cactcacctc tcccttcccc aacccttctc
     4081 tacttggctg ctgttttaaa gtttgccctt ccccaaattt ggatttttat tacagatcta
     4141 aagctctttc gattttatac tgattaaatc agtactgcag tatttgatta accaagcttc
     4201 tgcagatttt gtgattcttg ggactttttt gacgtaagaa atacttcttt atttatgcat
     4261 attcttccca cagtgatttt tccagcattc ttctgccata tgcctttagg gcttttataa
     4321 aatagaaaat taggcattct gatatttctt tagctgcttt gtgtgaaacc atggtgtaaa
     4381 agcacagctg gctgcttttt actgcttgtg tagtcacgag tccattgtaa tcatcacaat
     4441 tctaaaccaa actaccaata aagaaaacag acatccacca gtaagcaagc tctgttaggc
     4501 ttccatgtta gtgtagcttc tctcccacaa gttgtcctcc taggacaaga attatcttac
     4561 aaactaaact atcatcacac taccttgtat gccagcacct ggtaacagta gagattttta
     4621 tacattaatc ttgatctgtt ttaatcttga tctgttttag tagagatttt tatacattaa
     4681 tcttgatctg ttttaatctt gatctgtttt gtcctagaaa attccatcat acacacattt
     4741 cctgatattt gggcttagtg cttctaaatt gttgcagaca caaaacttaa tgattcattc
     4801 gtagtagtaa tctgtagcta gttttagggt tttgctgaag tcagtgtggg gtgtttgttt
     4861 gaggaaaaag ttccaaatat ccactagcat agaattttaa actattttta ttttaaagtt
     4921 atggcataac atataacata aaaatatttt atatacgttt gaaaaatcta taccgttctt
     4981 ttttatcatc aaagtttctc aatggccagt agaagcaaaa agacaacacc acctctgatc
     5041 tacgggacat aatgttccca ggaaaaaaat cttcaagtgg gtgtgagggt gtttctaatt
     5101 caaaatatgt agatttctcc gcatggaaga agtagtaaag attttcttaa catgctcctg
     5161 tgttcatgct tggagacaag aataagatgg tttagaagct ttaccctttc ttggaacaag
     5221 tagaatcccg gtctgagacc tctcaggaat ttcagagctt agcagtctgg cctggaggtt
     5281 tttcagcctt ttagtataat aaacagtagt tgcttcctag agttgagttt ttaaggattc
     5341 acaaaaagga gcctgtacaa aatatacata cagttcttta ttaaacaact gtaaacactt
     5401 cactgtaaaa atccataaaa ctttataaac aaacattttg taaatagaat ctatgctaca
     5461 gtaaaataat taacacaatt atttacatgc aatactgaca aatttggcac tttttgaaaa
     5521 gaaatgtaca aaacacttgc tttaaaagaa atttaaaatt ataaaaactc cgagcattac
     5581 tatcatgcac tttgcaaata cctcacaagc acttatggca cagctatcag agagcatcag
     5641 gctctctggt aatatttatg taacttttaa tgtgcttcca taagtttgtt gtaaaaccac
     5701 ctggacattg tcaagaataa agtcaaatgc catattccaa a
//
