LOCUS       NM_198241               5405 bp    mRNA    linear   PRI 08-FEB-2025
DEFINITION  Homo sapiens eukaryotic translation initiation factor 4 gamma 1
            (EIF4G1), transcript variant 2, mRNA.
ACCESSION   NM_198241
VERSION     NM_198241.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Li,F., He,Z., Zhang,X., Gao,D., Xu,R., Zhang,Z., Cao,X., Shan,Q.,
            Liu,Y. and Xu,Z.
  TITLE     USP10 promotes cell proliferation, migration, and invasion in NSCLC
            through deubiquitination and stabilization of EIF4G1
  JOURNAL   Sci Rep 14 (1), 23685 (2024)
   PUBMED   39390016
  REMARK    GeneRIF: USP10 promotes cell proliferation, migration, and invasion
            in NSCLC through deubiquitination and stabilization of EIF4G1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Wu,S. and Wagner,G.
  TITLE     Computational inference of eIF4F complex function and structure in
            human cancers
  JOURNAL   Proc Natl Acad Sci U S A 121 (5), e2313589121 (2024)
   PUBMED   38266053
  REMARK    GeneRIF: Computational inference of eIF4F complex function and
            structure in human cancers.
REFERENCE   3  (bases 1 to 5405)
  AUTHORS   Clark,N.K., Harris,M.T., Dahl,W.B., Knotts,Z. and Marr,M.T. 2nd.
  TITLE     The Insulin Receptor and Insulin like Growth Factor Receptor 5'
            UTRs Support Translation Initiation Independently of EIF4G1
  JOURNAL   Mol Cell Biol 43 (10), 485-499 (2023)
   PUBMED   37724583
  REMARK    GeneRIF: The Insulin Receptor and Insulin like Growth Factor
            Receptor 5' UTRs Support Translation Initiation Independently of
            EIF4G1.
REFERENCE   4  (bases 1 to 5405)
  AUTHORS   Coldwell,M.J. and Morley,S.J.
  TITLE     Specific isoforms of translation initiation factor 4GI show
            differences in translational activity
  JOURNAL   Mol Cell Biol 26 (22), 8448-8460 (2006)
   PUBMED   16982693
  REMARK    GeneRIF: We quantify the efficiency of eIF4GI promoter usage in
            mammalian cells and demonstrate that even though the longest
            isoform eIF4GIf was relatively poorly expressed when reintroduced,
            it was more efficient at promoting the translation of cellular
            mRNAs.
REFERENCE   5  (bases 1 to 5405)
  AUTHORS   Byrd,M.P., Zamora,M. and Lloyd,R.E.
  TITLE     Translation of eukaryotic translation initiation factor 4GI
            (eIF4GI) proceeds from multiple mRNAs containing a novel
            cap-dependent internal ribosome entry site (IRES) that is active
            during poliovirus infection
  JOURNAL   J Biol Chem 280 (19), 18610-18622 (2005)
   PUBMED   15755734
  REMARK    GeneRIF: intact eIF4GI protein is not required for the de novo
            synthesis of eIF4GI, suggesting its expression can continue under
            stress or infection conditions where eIF4GI is cleaved
REFERENCE   6  (bases 1 to 5405)
  AUTHORS   Byrd,M.P., Zamora,M. and Lloyd,R.E.
  TITLE     Generation of multiple isoforms of eukaryotic translation
            initiation factor 4GI by use of alternate translation initiation
            codons
  JOURNAL   Mol Cell Biol 22 (13), 4499-4511 (2002)
   PUBMED   12052860
  REMARK    GeneRIF: data suggest that expression of the eIF4GI isoforms is
            partly controlled by a complex translation strategy involving both
            cap-dependent and cap-independent mechanisms
REFERENCE   7  (bases 1 to 5405)
  AUTHORS   Bradley,C.A., Padovan,J.C., Thompson,T.L., Benoit,C.A., Chait,B.T.
            and Rhoads,R.E.
  TITLE     Mass spectrometric analysis of the N terminus of translational
            initiation factor eIF4G-1 reveals novel isoforms
  JOURNAL   J Biol Chem 277 (15), 12559-12571 (2002)
   PUBMED   11821405
  REMARK    GeneRIF: mass spectrometric analysis of N terminus reveals novel
            isoforms
REFERENCE   8  (bases 1 to 5405)
  AUTHORS   Mader,S., Lee,H., Pause,A. and Sonenberg,N.
  TITLE     The translation initiation factor eIF-4E binds to a common motif
            shared by the translation factor eIF-4 gamma and the translational
            repressors 4E-binding proteins
  JOURNAL   Mol Cell Biol 15 (9), 4990-4997 (1995)
   PUBMED   7651417
REFERENCE   9  (bases 1 to 5405)
  AUTHORS   Yan,R. and Rhoads,R.E.
  TITLE     Human protein synthesis initiation factor eIF-4 gamma is encoded by
            a single gene (EIF4G) that maps to chromosome 3q27-qter
  JOURNAL   Genomics 26 (2), 394-398 (1995)
   PUBMED   7601469
REFERENCE   10 (bases 1 to 5405)
  AUTHORS   Yan,R., Rychlik,W., Etchison,D. and Rhoads,R.E.
  TITLE     Amino acid sequence of the human protein synthesis initiation
            factor eIF-4 gamma
  JOURNAL   J Biol Chem 267 (32), 23226-23231 (1992)
   PUBMED   1429670
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC078797.20.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_198241.2.
            
            Summary: The protein encoded by this gene is a component of the
            multi-subunit protein complex EIF4F. This complex facilitates the
            recruitment of mRNA to the ribosome, which is a rate-limiting step
            during the initiation phase of protein synthesis. The recognition
            of the mRNA cap and the ATP-dependent unwinding of 5'-terminal
            secondary structure is catalyzed by factors in this complex. The
            subunit encoded by this gene is a large scaffolding protein that
            contains binding sites for other members of the EIF4F complex. A
            domain at its N-terminus can also interact with the poly(A)-binding
            protein, which may mediate the circularization of mRNA during
            translation. Alternative splicing results in multiple transcript
            variants, some of which are derived from alternative promoter
            usage. [provided by RefSeq, Aug 2010].
            
            Transcript Variant: This variant (2) differs in the 5' UTR and uses
            an alternate in-frame splice site in the central coding region,
            compared to variant 1, resulting in an isoform (5) that is 1 aa
            shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX647812.1, SRR7346977.2441908.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000346169.7/ ENSP00000316879.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-69                AC078797.20        55971-56039
            70-126              AC078797.20        56854-56910
            127-220             AC078797.20        57128-57221
            221-307             AC078797.20        57497-57583
            308-484             AC078797.20        58686-58862
            485-584             AC078797.20        59082-59181
            585-697             AC078797.20        61054-61166
            698-790             AC078797.20        61995-62087
            791-857             AC078797.20        62292-62358
            858-1679            AC078797.20        62647-63468
            1680-1768           AC078797.20        63727-63815
            1769-1955           AC078797.20        63909-64095
            1956-2089           AC078797.20        64186-64319
            2090-2248           AC078797.20        64448-64606
            2249-2434           AC078797.20        64773-64958
            2435-2632           AC078797.20        65145-65342
            2633-2779           AC078797.20        65566-65712
            2780-3016           AC078797.20        66243-66479
            3017-3121           AC078797.20        66634-66738
            3122-3281           AC078797.20        66845-67004
            3282-3382           AC078797.20        67216-67316
            3383-3485           AC078797.20        67892-67994
            3486-3588           AC078797.20        68246-68348
            3589-3821           AC078797.20        68581-68813
            3822-3940           AC078797.20        68951-69069
            3941-4113           AC078797.20        69195-69367
            4114-4239           AC078797.20        69996-70121
            4240-4321           AC078797.20        70274-70355
            4322-4420           AC078797.20        72631-72729
            4421-4555           AC078797.20        72837-72971
            4556-4637           AC078797.20        73093-73174
            4638-4778           AC078797.20        73311-73451
            4779-5405           AC078797.20        76092-76718
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q27.1"
     gene            1..5405
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /note="eukaryotic translation initiation factor 4 gamma 1"
                     /db_xref="GeneID:1981"
                     /db_xref="HGNC:HGNC:3296"
                     /db_xref="MIM:600495"
     exon            1..69
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            70..126
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            127..220
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..4960
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /note="isoform 5 is encoded by transcript variant 2;
                     EIF4-gamma; eucaryotic translation initiation factor 4G;
                     eIF-4-gamma 1"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 4 gamma
                     1 isoform 5"
                     /protein_id="NP_937884.2"
                     /db_xref="CCDS:CCDS3259.1"
                     /db_xref="GeneID:1981"
                     /db_xref="HGNC:HGNC:3296"
                     /db_xref="MIM:600495"
                     /translation="MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTP
                     SQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGA
                     YYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAP
                     TPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQ
                     ANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGS
                     EPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKP
                     VPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPV
                     PIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATS
                     PAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVA
                     VSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPR
                     PEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIF
                     ASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRG
                     PPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKR
                     TAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVI
                     DLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKD
                     DDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEA
                     IMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTS
                     SRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDK
                     RRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPG
                     GRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSL
                     SRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGL
                     RKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEA
                     VQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLY
                     EILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLE
                     ILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQR
                     ALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFE
                     TPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDE
                     DVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN"
     exon            221..307
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            308..484
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            485..584
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            585..697
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            698..790
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            791..857
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            858..1679
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            1680..1768
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            1769..1955
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2089
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            2090..2248
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            2249..2434
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            2435..2632
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            2633..2779
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            2780..3016
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            3017..3121
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            3122..3281
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            3282..3382
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            3383..3485
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            3486..3588
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            3589..3821
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            3822..3940
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            3941..4113
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            4114..4239
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            4240..4321
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            4322..4420
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            4421..4555
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            4556..4637
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            4638..4778
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     exon            4779..5405
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5385..5390
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /note="hexamer: ATTAAA"
     polyA_site      5405
                     /gene="EIF4G1"
                     /gene_synonym="EIF-4G1; EIF4F; EIF4G; EIF4GI; P220;
                     PARK18"
                     /note="major polyA site"
ORIGIN      
        1 ggaaatggtt gtggactacg tctgtgcggc tgcgtggggc tcggccgcgc ggactgaagg
       61 agactgaagg ccctcggatg cccagaacct gtaggccgca ccgtggactt gttcttaatc
      121 gagggggtgc tggggggacc ctgatgtggc accaaatgaa atgaacaaag ctccacagtc
      181 cacaggcccc ccacccgccc catcccccgg actcccacag ccagcgtttc ccccggggca
      241 gacagcgccg gtggtgttca gtacgccaca agcgacacaa atgaacacgc cttctcagcc
      301 ccgccagcac ttctacccta gccgggccca gcccccgagc agtgcagcct cccgagtgca
      361 gagtgcagcc cctgcccgcc ctggcccagc tgcccatgtc taccctgctg gatcccaagt
      421 aatgatgatc ccttcccaga tctcctaccc agcctcccag ggggcctact acatccctgg
      481 acaggggcgt tccacatacg ttgtcccgac acagcagtac cctgtgcagc caggagcccc
      541 aggcttctat ccaggtgcaa gccctacaga atttgggacc tacgctggcg cctactatcc
      601 agcccaaggg gtgcagcagt ttcccactgg cgtggccccc accccagttt tgatgaacca
      661 gccaccccag attgctccca agagggagcg taagacgatc cgaattcgag atccaaacca
      721 aggaggaaag gatatcacag aggagatcat gtctggggcc cgcactgcct ccacacccac
      781 ccctccccag acgggaggcg gtctggagcc tcaagctaat ggggagacgc cccaggttgc
      841 tgtcattgtc cggccagatg accggtcaca gggagcaatc attgctgacc ggccagggct
      901 gcctggccca gagcatagcc cttcagaatc ccagccttcg tcgccttctc cgaccccatc
      961 accatcccca gtcttggaac cggggtctga gcctaatctc gcagtcctct ctattcctgg
     1021 ggacactatg acaactatac aaatgtctgt agaagaatca acccccatct cccgtgaaac
     1081 tggggagcca tatcgcctct ctccagaacc cactcctctc gccgaaccca tactggaagt
     1141 agaagtgaca cttagcaaac cggttccaga atctgagttt tcttccagtc ctctccaggc
     1201 tcccacccct ttggcatctc acacagtgga aattcatgag cctaatggca tggtcccatc
     1261 tgaagatctg gaaccagagg tggagtcaag cccagagctt gctcctcccc cagcttgccc
     1321 ctccgaatcc cctgtgccca ttgctccaac tgcccaacct gaggaactgc tcaacggagc
     1381 cccctcgcca ccagctgtgg acttaagccc agtcagtgag ccagaggagc aggccaagga
     1441 ggtgacagca tcaatggcgc cccccaccat cccctctgct actccagcta cggctccttc
     1501 agctacttcc ccagctcagg aggaggaaat ggaagaagaa gaagaagagg aagaaggaga
     1561 agcaggagaa gcaggagaag ctgagagtga gaaaggagga gaggaactgc tccccccaga
     1621 gagtacccct attccagcca acttgtctca gaatttggag gcagcagcag ccactcaagt
     1681 ggcagtatct gtgccaaaga ggagacggaa aattaaggag ctaaataaga aggaggctgt
     1741 tggagacctt ctggatgcct tcaaggaggc gaacccggca gtaccagagg tggaaaatca
     1801 gcctcctgca ggcagcaatc caggcccaga gtctgagggc agtggtgtgc ccccacgtcc
     1861 tgaggaagca gatgagacct gggactcaaa ggaagacaaa attcacaatg ctgagaacat
     1921 ccagcccggg gaacagaagt atgaatataa gtcagatcag tggaagcctc taaacctaga
     1981 ggagaaaaaa cgttacgacc gtgagttcct gcttggtttt cagttcatct ttgccagtat
     2041 gcagaagcca gagggattgc cacatatcag tgacgtggtg ctggacaagg ccaataaaac
     2101 accactgcgg ccactggatc ccactagact acaaggcata aattgtggcc cagacttcac
     2161 tccatccttt gccaaccttg gccggacaac ccttagcacc cgtgggcccc caaggggtgg
     2221 gccaggtggg gagctgcccc gtgggccggc tggcctggga ccccggcgct ctcagcaggg
     2281 accccgaaaa gaaccacgca agatcattgc cacagtgtta atgaccgaag atataaaact
     2341 gaacaaagca gagaaagcct ggaaacccag cagcaagcgg acggcggctg ataaggatcg
     2401 aggggaagaa gatgctgatg gcagcaaaac ccaggaccta ttccgcaggg tgcgctccat
     2461 cctgaataaa ctgacacccc agatgttcca gcagctgatg aagcaagtga cgcagctggc
     2521 catcgacacc gaggaacgcc tcaaaggggt cattgacctc atttttgaga aggccatttc
     2581 agagcccaac ttctctgtgg cctatgccaa catgtgccgc tgcctcatgg cgctgaaagt
     2641 gcccactacg gaaaagccaa cagtgactgt gaacttccga aagctgttgt tgaatcgatg
     2701 tcagaaggag tttgagaaag acaaagatga tgatgaggtt tttgagaaga agcaaaaaga
     2761 gatggatgaa gctgctacgg cagaggaacg aggacgcctg aaggaagagc tggaagaggc
     2821 tcgggacata gcccggcggc gctctttagg gaatatcaag tttattggag agttgttcaa
     2881 actgaagatg ttaacagagg caataatgca tgactgtgtg gtcaaactgc ttaagaacca
     2941 tgatgaagag tcccttgagt gcctttgtcg tctgctcacc accattggca aagacctgga
     3001 ctttgaaaaa gccaagcccc gaatggatca gtatttcaac cagatggaaa aaatcattaa
     3061 agaaaagaag acgtcatccc gcatccgctt tatgctgcag gacgtgctgg atctgcgagg
     3121 gagcaattgg gtgccacgcc gaggggatca gggtcccaag accattgacc agatccataa
     3181 ggaggctgag atggaagaac atcgagagca catcaaagtg cagcagctca tggccaaggg
     3241 cagtgacaag cgtcggggcg gtcctccagg ccctcccatc agccgtggac ttccccttgt
     3301 ggatgatggt ggctggaaca cagttcccat cagcaaaggt agccgcccca ttgacacctc
     3361 acgactcacc aagatcacca agcctggctc catcgattct aacaaccagc tctttgcacc
     3421 tggagggcga ctgagctggg gcaagggcag cagcggaggc tcaggagcca agccctcaga
     3481 cgcagcatca gaagctgctc gcccagctac tagtactttg aatcgcttct cagcccttca
     3541 acaagcggta cccacagaaa gcacagataa tagacgtgtg gtgcagagga gtagcttgag
     3601 ccgagaacga ggcgagaaag ctggagaccg aggagaccgc ctagagcgga gtgaacgggg
     3661 aggggaccgt ggggaccggc ttgatcgtgc gcggacacct gctaccaagc ggagcttcag
     3721 caaggaagtg gaggagcgga gtagagaacg gccctcccag cctgaggggc tgcgcaaggc
     3781 agctagcctc acggaggatc gggaccgtgg gcgggatgcc gtgaagcgag aagctgccct
     3841 acccccagtg agccccctga aggcggctct ctctgaggag gagttagaga agaaatccaa
     3901 ggctatcatt gaggaatatc tccatctcaa tgacatgaaa gaggcagtcc agtgcgtgca
     3961 ggagctggcc tcaccctcct tgctcttcat ctttgtacgg catggtgtcg agtctacgct
     4021 ggagcgcagt gccattgctc gtgagcatat ggggcagctg ctgcaccagc tgctctgtgc
     4081 tgggcatctg tctactgctc agtactacca agggttgtat gaaatcttgg aattggctga
     4141 ggacatggaa attgacatcc cccacgtgtg gctctaccta gcggaactgg taacacccat
     4201 tctgcaggaa ggtggggtgc ccatggggga gctgttcagg gagattacaa agcctctgag
     4261 accgttgggc aaagctgctt ccctgttgct ggagatcctg ggcctcctgt gcaaaagcat
     4321 gggtcctaaa aaggtgggga cgctgtggcg agaagccggg cttagctgga aggaatttct
     4381 acctgaaggc caggacattg gtgcattcgt cgctgaacag aaggtggagt ataccctggg
     4441 agaggagtcg gaagcccctg gccagagggc actcccctcc gaggagctga acaggcagct
     4501 ggagaagctg ctgaaggagg gcagcagtaa ccagcgggtg ttcgactgga tagaggccaa
     4561 cctgagtgag cagcagatag tatccaacac gttagttcga gccctcatga cggctgtctg
     4621 ctattctgca attatttttg agactcccct ccgagtggac gttgcagtgc tgaaagcgcg
     4681 agcgaagctg ctgcagaaat acctgtgtga cgagcagaag gagctacagg cgctctacgc
     4741 cctccaggcc cttgtagtga ccttagaaca gcctcccaac ctgctgcgga tgttctttga
     4801 cgcactgtat gacgaggacg tggtgaagga ggatgccttc tacagttggg agagtagcaa
     4861 ggaccccgct gagcagcagg gcaagggtgt ggcccttaaa tctgtcacag ccttcttcaa
     4921 gtggctccgt gaagcagagg aggagtctga ccacaactga gggctggtgg ggccggggac
     4981 ctggagcccc atggacacac agatggcccg gctagccgcc tggactgcag gggggcggca
     5041 gcagcggcgg tggcagtggg tgcctgtagt gtgatgtgtc tgaactaata aagtggctga
     5101 agaggcagga tggcttgggg ctgcctgggc ccccctccag gatgccgcca ggtgtccctc
     5161 tcctccccct ggggcacaga gatatattat atataaagtc ttgaaatttg gtgtgtcttg
     5221 gggtggggag gggcaccaac gcctgcccct ggggtccttt tttttatttt ctgaaaatca
     5281 ctctcgggac tgccgtcctc gctgctgggg gcatatgccc cagcccctgt accacccctg
     5341 ctgttgcctg ggcaggggga agggggggca cggtgcctgt aattattaaa catgaattca
     5401 attaa
//
