LOCUS       NM_001134651            9978 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens eukaryotic translation initiation factor 4E family
            member 3 (EIF4E3), transcript variant 1, mRNA.
ACCESSION   NM_001134651
VERSION     NM_001134651.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Xu,X., Zhao,Y., Ying,Y., Zhu,H., Luo,J., Mou,T. and Zhang,Z.
  TITLE     m7G-related genes-NCBP2 and EIF4E3 determine immune contexture in
            head and neck squamous cell carcinoma by regulating CCL4/CCL5
            expression
  JOURNAL   Mol Carcinog 62 (8), 1091-1106 (2023)
   PUBMED   37067401
  REMARK    GeneRIF: m7G-related genes-NCBP2 and EIF4E3 determine immune
            contexture in head and neck squamous cell carcinoma by regulating
            CCL4/CCL5 expression.
REFERENCE   2  (bases 1 to 9978)
  AUTHORS   Weiss,B., Allen,G.E., Kloehn,J., Abid,K., Jaquier-Gubler,P. and
            Curran,J.A.
  TITLE     eIF4E3 forms an active eIF4F complex during stresses (eIF4FS)
            targeting mTOR and re-programs the translatome
  JOURNAL   Nucleic Acids Res 49 (9), 5159-5176 (2021)
   PUBMED   33893802
  REMARK    GeneRIF: eIF4E3 forms an active eIF4F complex during stresses
            (eIF4FS) targeting mTOR and re-programs the translatome.
REFERENCE   3  (bases 1 to 9978)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 9978)
  AUTHORS   Mrvova,S., Frydryskova,K., Pospisek,M., Vopalensky,V. and Masek,T.
  TITLE     Major splice variants and multiple polyadenylation site utilization
            in mRNAs encoding human translation initiation factors eIF4E1 and
            eIF4E3 regulate the translational regulators?
  JOURNAL   Mol Genet Genomics 293 (1), 167-186 (2018)
   PUBMED   28942592
  REMARK    GeneRIF: We performed bioinformatics analyses of ESTs and the
            3'UTRs of the main transcript splice variants of the translational
            initiation factor eIF4E1 and its family members, eIF4E2 and eIF4E3.
            We propose to elucidate the minor splice variants of eIF4E2 and
            eIF4E3 in great detail because they might produce proteins with
            modified features that fulfill different cellular roles from their
            major counterparts.
REFERENCE   5  (bases 1 to 9978)
  AUTHORS   Frydryskova,K., Masek,T., Borcin,K., Mrvova,S., Venturi,V. and
            Pospisek,M.
  TITLE     Distinct recruitment of human eIF4E isoforms to processing bodies
            and stress granules
  JOURNAL   BMC Mol Biol 17 (1), 21 (2016)
   PUBMED   27578149
  REMARK    GeneRIF: Following heat shock, eIF4E2 is found in both processing
            bodies and stress granules, whereas eIF4E3_A relocates only to
            stress granules.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9978)
  AUTHORS   Landon,A.L., Muniandy,P.A., Shetty,A.C., Lehrmann,E., Volpon,L.,
            Houng,S., Zhang,Y., Dai,B., Peroutka,R., Mazan-Mamczarz,K.,
            Steinhardt,J., Mahurkar,A., Becker,K.G., Borden,K.L. and
            Gartenhaus,R.B.
  TITLE     MNKs act as a regulatory switch for eIF4E1 and eIF4E3 driven mRNA
            translation in DLBCL
  JOURNAL   Nat Commun 5, 5413 (2014)
   PUBMED   25403230
  REMARK    GeneRIF: MNK1 and MNK2 inhibition ablates eIF4E1 phosphorylation
            and concurrently enhances eIF4E3 expression in diffuse large B-cell
            lymphoma.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 9978)
  AUTHORS   Volpon,L., Osborne,M.J., Culjkovic-Kraljacic,B. and Borden,K.L.
  TITLE     eIF4E3, a new actor in mRNA metabolism and tumor suppression
  JOURNAL   Cell Cycle 12 (8), 1159-1160 (2013)
   PUBMED   23587918
  REMARK    GeneRIF: eIF4E3 impedes oncogenic transformation and its expression
            is lost in some cancers.
REFERENCE   8  (bases 1 to 9978)
  AUTHORS   Osborne,M.J., Volpon,L., Kornblatt,J.A., Culjkovic-Kraljacic,B.,
            Baguet,A. and Borden,K.L.
  TITLE     eIF4E3 acts as a tumor suppressor by utilizing an atypical mode of
            methyl-7-guanosine cap recognition
  JOURNAL   Proc Natl Acad Sci U S A 110 (10), 3877-3882 (2013)
   PUBMED   23431134
REFERENCE   9  (bases 1 to 9978)
  AUTHORS   Joshi,B., Cameron,A. and Jagus,R.
  TITLE     Characterization of mammalian eIF4E-family members
  JOURNAL   Eur J Biochem 271 (11), 2189-2203 (2004)
   PUBMED   15153109
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC134770.2, DR159502.1, AK126999.1, AC097634.2 and AL161983.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001134651.1.
            
            Summary: EIF4E3 belongs to the EIF4E family of translational
            initiation factors that interact with the 5-prime cap structure of
            mRNA and recruit mRNA to the ribosome (Joshi et al., 2004 [PubMed
            15153109]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK126999.1, SRR18074969.1378412.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000425534.8/ ENSP00000393324.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                AC134770.2         42358-42384         c
            28-33               DR159502.1         1-6
            34-34               AC134770.2         42351-42351         c
            35-145              DR159502.1         7-117
            146-998             AK126999.1         1-853
            999-4542            AC097634.2         189736-193279       c
            4543-6103           AL161983.1         2960-4520
            6104-9978           AC097634.2         184300-188174       c
FEATURES             Location/Qualifiers
     source          1..9978
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p13"
     gene            1..9978
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /note="eukaryotic translation initiation factor 4E family
                     member 3"
                     /db_xref="GeneID:317649"
                     /db_xref="HGNC:HGNC:31837"
                     /db_xref="MIM:609896"
     exon            1..211
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..710
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /note="isoform a is encoded by transcript variant 1;
                     eukaryotic translation initiation factor 4E type 3"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 4E type
                     3 isoform a"
                     /protein_id="NP_001128123.1"
                     /db_xref="CCDS:CCDS46867.1"
                     /db_xref="GeneID:317649"
                     /db_xref="HGNC:HGNC:31837"
                     /db_xref="MIM:609896"
                     /translation="MALPPAAAPPAGAREPPGSRAAAAAAAPEPPLGLQQLSALQPEP
                     GGVPLHSSWTFWLDRSLPGATAAECASNLKKIYTVQTVQIFWSVYNNIPPVTSLPLRC
                     SYHLMRGERRPLWEEESNAKGGVWKMKVPKDSTSTVWKELLLATIGEQFTDCAAADDE
                     VIGVSVSVRDREDVVQVWNVNASLVGEATVLEKIYELLPHITFKAVFYKPHEEHHAFE
                     GGRGKH"
     exon            212..284
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /inference="alignment:Splign:2.1.0"
     exon            285..379
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /inference="alignment:Splign:2.1.0"
     exon            380..440
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /inference="alignment:Splign:2.1.0"
     exon            441..507
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /inference="alignment:Splign:2.1.0"
     exon            508..663
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /inference="alignment:Splign:2.1.0"
     exon            664..9978
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2642..2647
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /note="hexamer: AATAAA"
     polyA_site      2670
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /note="major polyA site"
     regulatory      3513..3518
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /note="hexamer: AATAAA"
     polyA_site      3536
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /note="major polyA site"
     regulatory      6065..6070
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /note="hexamer: ATTAAA"
     polyA_site      6097
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
     regulatory      9958..9963
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
                     /note="hexamer: ATTAAA"
     polyA_site      9978
                     /gene="EIF4E3"
                     /gene_synonym="eIF-4E3; eIF4E-3"
ORIGIN      
        1 gcgcgtccgc cggccttgca ggcggggcgg agaaaatggc gctgcccccg gccgccgcgc
       61 cccccgccgg ggcccgggag ccgccggggt cccgcgccgc cgccgctgcc gccgcccccg
      121 agccgccgct cggcctgcag cagctgtcgg cgctgcagcc tgagccgggc ggggtcccgc
      181 tgcactcgtc ctggaccttc tggctcgaca gatccctccc tggtgccaca gcagctgagt
      241 gcgcatcaaa tctgaagaaa atctacacag tacagacagt acagatattt tggagtgtat
      301 acaataatat ccctcctgtg actagcctgc ctttgagatg tagttatcat ttaatgagag
      361 gagagaggcg accactttgg gaagaggaga gtaatgcaaa gggtggcgta tggaagatga
      421 aagtccccaa ggacagcacg tccacagttt ggaaagagtt gctgttagca accatcgggg
      481 aacagttcac agactgtgcc gcagcagatg atgaagtaat aggagttagt gtcagtgttc
      541 gggaccgaga agacgtcgtc caagtctgga atgtaaatgc ctctttagtg ggtgaagcga
      601 ctgttttaga aaagatctat gaacttctgc cccacataac ttttaaagca gtattttata
      661 aaccccatga agagcatcat gcttttgaag gtggacgtgg aaaacactaa ttgcactctg
      721 taaagaattc tttgtccttt gctgattggt tttggaaacg gtcttaacag gagggagagt
      781 gaagagaaga cttgccgaag ccgatgctgg tcaatttaga gtgggtttct gtccttgcag
      841 atgggcaatt aggactcaaa gtggcaggtg gggtgggggg agattgtgtg ggtttctacc
      901 gtcacattac ttgcttttta aaaaaaacac acacaaaaaa cttttttttt ggctttctaa
      961 attctctgtt cttttcactc tgattttttt ttttttttgc cgtttaaaat tccttattca
     1021 gcctaattat tgttttctac actccccttt cattgaggca caagaaatgg gtttcccttt
     1081 aagtagctct gctgtaccta gttaatgaga atatgcataa tcgtgacaat actgcttttg
     1141 aataccacac tgtacaagga ggaacactag cttggagaaa tctgtcttct gattatttgt
     1201 tgtgacacat tcctatgtac tgtgagctgg gcattgtttt tttctgtccc agggaaaaga
     1261 aaaaccatac ttaatgtgat tttgcactga cagcacacaa atctttaatt ttccttttta
     1321 aaacattttt ttttagtgat aaaaggaaaa taatagttgg attgcataac atgaaaacta
     1381 taatcacaat tcagtatcag gatataaatc aagctctaag tctttggacc ctctgtggaa
     1441 acatttctaa cccgaatttt aatttggcct tttcaaccaa aggcttagag tggtagcaag
     1501 ggatttcttc caataggaaa cctgatgttt ctgatttaaa gagaaggtga tattttaatt
     1561 gtttgaatta agctccttgc aaagttcggg tgtgtttacc cacttagggc tttctttccc
     1621 agtcaacgct aacacatttt gttaaatgat ccctttccct gctcacattg tgtgtcattt
     1681 ctcatcatca gtagtcctcc agcctgggca gctgtcccca ccctttttca tgtaggtgca
     1741 ggaagttaaa tctcatttcc aggatgcatg tgaacattta caaagttgaa ctttgagtgc
     1801 attctgctca tatgaattat tgggattgtt gatatatatt gtattatgct accaaagaaa
     1861 tattggtttt attagaagga aatggtcatc ctctggacca tggagactag ctcagaatac
     1921 gctgatttcc ctctcctgac tttgccaagc ctttggctgc ttttgcctga taaagggcag
     1981 ggccatctga agacacttcc cccagtcggc tttggagtca cgggagctag tgcctgctca
     2041 cacatttttc aaaagggcag tgcactcaga acttcactgt acctgggatt tttaattcct
     2101 cttgcagtgt tgaccagcag agagacttga ggctacttta agcctccact atgtgtttgt
     2161 agataaaatt ctccattcaa acattttaaa ggactttgaa cattatctgc ttatggaagt
     2221 tgtgcccttc acttggttag taaccacctc agccataata cttaccatca taggtttctt
     2281 aaaatgcttt ttttttttcc ctaaacttga gtttccttag tgatttcaaa atgaagtata
     2341 agaatatcag atccagttag caaaagccta ggacttgttt ctccaaacat tgtactaaca
     2401 ttcaacttgt tttaaaatta tgactcaaga attttaaaaa attattctgg acatgaatta
     2461 aaactttttt ataatataag tatttttctg attgaaaaaa ggatataatt gacttcactc
     2521 taattgtcat gtatatttcc ataagtaaat ggattttgaa gtatttttat tttttgaact
     2581 ttatttaaag catttgtgat gacatgttca acttttgcat gtatgtagcc tttgaagtaa
     2641 aaataaatag gaatgttagg ctcacgttaa tatttctagt ttctgtttat gatcatggaa
     2701 catgactagc acacaaagta gatttttgcc ccagtgaact tctgcaactg tttctgaccc
     2761 tttagaaaat accaactgtg agaaggattt aatttgccta atgttaaaaa cctatcatct
     2821 aaattacttt cagagaggat tttgtcagat tttggtttgg aaattgtttt aattgaatat
     2881 ttaatcataa tagactttgt atgaactaag ctgatttaat cagtttaaaa acctctcatt
     2941 accttcttag attaaagaag ctaatactgt tttgagatag aaggaaatat tttgtggggc
     3001 atgtgacttt tgtgttaagg cagtttaccc atcttggctg atgtggggaa agtgcaaact
     3061 tccagtagaa tcccggtctt ttcatccaag tgcttgcagg ggcagccttg tagtctgcaa
     3121 agaaccatgc agaaaccaaa gagcttgctt ttttgtcttt gtaattcctt agggtctgtg
     3181 atatatgcca ggaaatctga ttgaaactaa tatgcttttt attctcccat tattccctaa
     3241 attgttatat cacaggcact tgcctacatt gggaaagtaa ggacaaataa tacccattta
     3301 aagtaacttg cctatatttt gactgtcttt cgtaagcact tgctgaactg ctgacttaac
     3361 tcagttttaa gatttcctct ttccccccaa cttttccaca tttcttaaat gagggtgtaa
     3421 tttactgcaa acatgtacat ttaatataat attgctgtta gattcgtggt acactttatg
     3481 aagcaaaaac atgaaaagga aaccacaact ctaataaact ttaaattaaa gattaagatg
     3541 actctttctc acccctcttc ttgagtttag cccatgtcta caccatgcac acagtcacaa
     3601 gactttatag cttagaaact ttaggtataa ttgggtccta tagacataat atacaatcca
     3661 ccaactgagc cttggccgaa cttgtccctt gttaaagacg tagggctagc cagccacaga
     3721 gctgggtatt gaagtcggca agttgtatcc cggtgcaggc gtccttaaat ggagagtact
     3781 cagaatctgc cagccttaac ctgcccacta gccaccctaa tcaagcatta aatatctgat
     3841 ccaatctggc cactggtgta actccgtaat cagacatcct gtttgtaaac agctcctcct
     3901 tctctccctc tccctctcag agacagaagt ggtgctgggg tttggagttt gaaaataacc
     3961 ttcctatgac aggaagtggg cttttgctat tagttgccca actcttagag gatcccagct
     4021 tgtgtcttta gacaaaactg acagcctttg gaacgatcgc atggtggcgc tgccttccag
     4081 ccaacagttc tggctcagca gcagaattta tccagctatg tttgtagaat ttttttaaga
     4141 tcctactgca ttttgctctt aagcttttat aaaaatttga cgagaaaatg taaaaatgat
     4201 acttggcaat aagacattta gttttcatgc acctaataaa aatgtgtcaa agctagatcg
     4261 ctccagatgt gcttaaaata tctcaaatcc taggctctca ttggccttat aagtgattgg
     4321 gaagaaaaag aataaaataa actcacatat aggagatatg gaaaatattt catcaaatat
     4381 tatgcccaaa gagagaaaat taggagttga ttttttttag cactctgaat atggaacttc
     4441 cttttaaaaa tgtaattcta cccttcacct ttcctacatt gtatgtttct gtgagatttt
     4501 actcattaca agtgaaagta gaggactgcc tgtgaattgt tcacttctga aaagaatata
     4561 ttgagatcag ttaaaattac tcaagagttt ggtgactcag gattgtaaaa agcctggatc
     4621 tcaacagctg ggaggagtca gagggtgaag tgggaaaaaa tagggactga gaggcaacga
     4681 aaagtccctt tgaacctgtg gtcatttata gcaattagag tgggttgccc ctctacttct
     4741 cccaagaaga aatcccaaat agattccttt catgtcagat tatactgttc tgattttatt
     4801 ttctattatt tttacatata catccaaatc taagaatact tattcagatt ggcccactca
     4861 ggccaaaagt cactgtcttg tgttctagaa attctttcat gttgctgaca taaatccatt
     4921 tttaaaagaa cattcaggga ctcttagaat tttagagtgg aagggacctt tgagatcatc
     4981 taccagtgct tctcacccct tagcatgcag caaaatcact cagatactta cttattattc
     5041 acatttcttg accccagcac tagaaatctg ggaggatatc tgcattttta acaagtgccc
     5101 tagtgattct aatgctgatg gtgctcagat cgctcttgga gaacccctga ttttatccaa
     5161 ctcccacata tgacaaatga ggaaagcctc ccaaccctaa gctctgtcag gtgacctgat
     5221 tccaatgtat gataccaaca atcttatgaa ggctggagaa aaccattaat actgagactt
     5281 gagtagatga tgctcgtgaa taagaacttt atggctggga ccacagcagt catgggagcc
     5341 catccctgga agtccataat ataaagcctt taatacagag tggtggtttt tctcttttct
     5401 aaataccatt tcagtctgtt aagagaaaag aaattagtgt tcagaaaagt agttactcca
     5461 tatgtgcagt aactactttt ctataatgtc ttatggcatc tggggttcag catcattgaa
     5521 caagattaaa tgaacgttat agagctataa aattgcatgt aaaataacac aaaactgttc
     5581 aaatgactat caaagtagcc tgggaatgaa tttccactaa aattaattgt cactttcaaa
     5641 acgtagctta gcaaagctgt tactaaactg gagagatcct ttaatacata gaaatgtcaa
     5701 aaaaatcaaa taagtattcc ctaaacgatg tgagtgataa gcaaaagtca ttgcaaagtc
     5761 agtatttttt tgttcatcat tgctactgaa attcagagtt ccattgtata tgtgtgtctt
     5821 gagtgtacct tattagaata tccaatacaa tagcttttgg atattctatg cttttgtgtc
     5881 cttcagaaat catgagattg aaggaatctg tgttaaatgt ttttgttttg gttgctgcct
     5941 gaaaaaagtt accaactcat ttagtgaaat gcaagatttc tttatgaaaa tttcaatttc
     6001 taatatcgaa gtcagataat ttttcttgaa cttttatttt ggaacagatg tttatttcgg
     6061 atctattaaa gtgtcaactt tgcattatag aactgaacat gaagtaaatt gagtgtttct
     6121 gagttttcta tagaaaattt atgctacctc tcatcttttc acatacaaat aagcaacttt
     6181 taatgaaggc ttagggtgaa aattttaatt ttagcacttt acattacatg atgaatggtg
     6241 aatattaaag gactttgaac attttaataa acttacagtg tttctgtttt caaatagagg
     6301 tattgatgct gtggatatac attaaattct ccccaaagtt ctcagacttt aatcagataa
     6361 tgtggcttac aaccattgtt tttgttttta agttttaaca ctgtcttttc agtattcatt
     6421 taaaacaagt caccatgact ttgaatatat tctcatggag aatttatttc aatgattata
     6481 taaaagaatt tcaaaattta aactagtgag catactttag cttgagacag ctaacattat
     6541 ttgtcccaaa tttaaaatct tacattgaaa gttattaatt ctttagaata gtcttacctg
     6601 tgtaagttga accaggtttg tttgattatg ttttggatct gcttgtgccc ccacccccct
     6661 attttatacc atctgtgccc tgtgcagttg gggacccttt cacatctggc tacaagctgg
     6721 tgtaatgccc ttccttttga tctggcttca tctagtgatt tgctcagtat ttttaaggtc
     6781 agactgaaaa cttttctgtt ctagttattt ttgattttat attgtgtgtt gtgtttgatg
     6841 ttcagataca tgtgaaagtt gcttctataa gtcagtattt tttattccag ccttttgcag
     6901 caataaggta agactagact atacttttct tacttgaaaa ggtagtgatt tatgtgttta
     6961 gtgcaaatgt ttagactgtt ttgcaatttt ctaatttggg actatattgc tatgaatttg
     7021 tgcttcttca tttttcaggg tagtagttca cagggtttgt ttaagtgtta gtttgtagaa
     7081 ctttattttg agatcttttg taatctgttg ctactaattc tcattctgta ttgaggttct
     7141 tatagtatag aaatgtcatt ttcatcctcc agtttaaaaa tttcatgcag tttaatcaac
     7201 cacatcactt aatcattatt agatttccag tgtgtgtgtg gtgattagaa taaccttgtt
     7261 tattacaaca catattaaaa gtttctgtcc aacacctcta atatgtgtga cgctgctagc
     7321 atggagtctg acgcttagta ggttatctgg tattctgatg gggagtaact gttgttattt
     7381 ttacacttcc ctgttgaaca catttgataa tagggctcat ctgggtctct tagaaaacgt
     7441 atcttgctta ttttaattct ttttctttac cctctattaa gaatggttcc gttttagaat
     7501 aagtcaatta ctctcgttgc tcttttgtgc tgcaatttgc tggtgagatt cagttgtccc
     7561 ctgtttcggc aagtaaagtg aatttactcc tcctttctgg ctgtctaata tattccttta
     7621 aaaacaaaca aaacgtacca acaaactctt cttccattca tctgcagact ttcctatctc
     7681 tccctgcaga aacaagcaat tcaggatctt atgtgtgatg cctaattaat tttaacacag
     7741 cgaatttaat gaaatcgttg aagagcaggg cataacacta ggaaggcttt aaaatttcat
     7801 aatgtttccc tctcatattt gcatctgatt actgccacca tgcattctgt gtgtacttta
     7861 tcatctagtc gttattgatg cactttttgg aatgtgttct cggttgttct attagcatag
     7921 gttcctctgc cttgtcacct aacgtacagt tttcttggaa gccaagacca tgcatgcctt
     7981 ttcttcctca gtgatccttt cgctgcagta actgctatta aagaactttt aactgctttt
     8041 cgttaatgaa tgcataaaaa aatctctgga atcctacaag acgatggttc ttttgttaca
     8101 tgaagaaaag aaaaaacagt agcatttgca agggccagca agttaaaata tatgtcatta
     8161 aaatatgttt ctatttccca tcatctggct aggctgtggt gctgactagc ttatttcttg
     8221 ccagagaaaa taagtttaaa tcataagcat atttaactag caagcataaa tcataaattc
     8281 tttataatcg taagcatgtt gaactctggt accatttgta cagatggcag ggctaaatgt
     8341 gtgtgctcct ctaagcatta aagatgtgga caagctctta ctccccaatt tcctctttaa
     8401 ctcaggaaac ttaaatcatt tgttttttgc acatatagga ttatataaag tatggaccta
     8461 gagaaaggtt tcagatctta ggaaacattt gagttttgtg attcatgctt actggaaaag
     8521 aatgatggaa gtgaaggtta aacactaaag ccgtgtcccc tttttaaggc gaaaataatt
     8581 agtgtttcat gaagatagtc aagttataaa ggacaagact gtttttcaga attttttttt
     8641 ttttttgaaa acaaacatct tggaatccac attttaacag aggtccatca gctttcaaag
     8701 agtgttccct agaagggtgg ttccacagaa acactttcag atatggcaaa gttttcattg
     8761 taaaatacat tttaagaagt atttttaaaa gtgtaataaa gcaataacac acagtgacat
     8821 gcatcacatg gccaaatttt aacattttgg aagccagtaa gtataactta tgtactcact
     8881 gattttgttt tatgaaatga aacaaaggtt ttataaaacc tttttattgt aaaataaagg
     8941 tacagaaaac cacacaaaac aaatgtgtaa cttactgaat tgttacaagg agaacactct
     9001 ataacagcag gccaagaaat agaactttgc cggtcacccc aggagccctt tcttgtgtcc
     9061 ccgccctctc cactaaaata accattatcc tgacctacag gagacacttg catttctctc
     9121 tggttttatc acaggtgata aacatcattt tacatataga atcttctcta actatgaacg
     9181 ttggttcaga aaacttattt gtaagtcttt taaatgatga gtgcaaaatg acacactata
     9241 agcaattgat acccccgtaa gttagtaact gggcagaatt taatgtgctt ttgctgtagt
     9301 gtgtggctct gtccatagct ataatatcca caataacaat agcatgtatg gagttccatg
     9361 ccatctaagc atagaggtta gttctcccca acactctacg aggtagatgc tgtcattgtt
     9421 gtccccagtt tgtgtatgtg gaaacatatg catgaggttc agagaggtga aataacttgc
     9481 cgaaggtcac accagtagtc agttgcagaa ccaagataca tctaaggtac tgtggctcca
     9541 gcctgttctt cagtgaggct gccttggtgg aagttgaaga tatgatgtgc aagaattttg
     9601 agattcctta tgagagtttg tttacaaaga aaaggtttta cagctttaag aaaaagatgg
     9661 aaaaatcact ggcctagttc aaacttttca ttttacagat gaagaaatga cccagaaagt
     9721 ataaagaatt cgttcaaggt cacacaagta gttggtggca aagttgcagc tatatcccaa
     9781 atctcctggc tgttaaaatc aatgatcttt tcttcattcc atatattatg tggaaaaaat
     9841 gcatgttttc taacctgctt ctttgcgaca actccatgta agcagatttg tcttaaaacc
     9901 agtttatagc taggacacac catctacttg cttattttct caaggtttta actgggaatt
     9961 aaaaaattta tatctgaa
//
