LOCUS       NM_003758               2479 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens eukaryotic translation initiation factor 3 subunit J
            (EIF3J), transcript variant 1, mRNA.
ACCESSION   NM_003758
VERSION     NM_003758.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Zheng,J.Y., Zhu,T., Zhuo,W., Mao,X.Y., Yin,J.Y., Li,X., He,Y.J.,
            Zhang,W., Liu,C. and Liu,Z.Q.
  TITLE     eIF3a sustains non-small cell lung cancer stem cell-like properties
            by promoting YY1-mediated transcriptional activation of
            beta-catenin
  JOURNAL   Biochem Pharmacol 213, 115616 (2023)
   PUBMED   37211173
  REMARK    GeneRIF: eIF3a sustains non-small cell lung cancer stem cell-like
            properties by promoting YY1-mediated transcriptional activation of
            beta-catenin.
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 2479)
  AUTHORS   Egorova,T., Biziaev,N., Shuvalov,A., Sokolova,E., Mukba,S.,
            Evmenov,K., Zotova,M., Kushchenko,A., Shuvalova,E. and Alkalaeva,E.
  TITLE     eIF3j facilitates loading of release factors into the ribosome
  JOURNAL   Nucleic Acids Res 49 (19), 11181-11196 (2021)
   PUBMED   34591963
  REMARK    GeneRIF: eIF3j facilitates loading of release factors into the
            ribosome.
REFERENCE   4  (bases 1 to 2479)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 2479)
  AUTHORS   Poh,J., Ponsford,A.H., Boyd,J., Woodsmith,J., Stelzl,U., Wanker,E.,
            Harper,N., MacEwan,D. and Sanderson,C.M.
  TITLE     A functionally defined high-density NRF2 interactome reveals new
            conditional regulators of ARE transactivation
  JOURNAL   Redox Biol 37, 101686 (2020)
   PUBMED   32911434
REFERENCE   6  (bases 1 to 2479)
  AUTHORS   Fraser,C.S., Lee,J.Y., Mayeur,G.L., Bushell,M., Doudna,J.A. and
            Hershey,J.W.
  TITLE     The j-subunit of human translation initiation factor eIF3 is
            required for the stable binding of eIF3 and its subcomplexes to 40
            S ribosomal subunits in vitro
  JOURNAL   J Biol Chem 279 (10), 8946-8956 (2004)
   PUBMED   14688252
REFERENCE   7  (bases 1 to 2479)
  AUTHORS   Mayeur,G.L., Fraser,C.S., Peiretti,F., Block,K.L. and Hershey,J.W.
  TITLE     Characterization of eIF3k: a newly discovered subunit of mammalian
            translation initiation factor elF3
  JOURNAL   Eur J Biochem 270 (20), 4133-4139 (2003)
   PUBMED   14519125
REFERENCE   8  (bases 1 to 2479)
  AUTHORS   Valasek,L., Hasek,J., Nielsen,K.H. and Hinnebusch,A.G.
  TITLE     Dual function of eIF3j/Hcr1p in processing 20 S pre-rRNA and
            translation initiation
  JOURNAL   J Biol Chem 276 (46), 43351-43360 (2001)
   PUBMED   11560931
REFERENCE   9  (bases 1 to 2479)
  AUTHORS   Bushell,M., Wood,W., Clemens,M.J. and Morley,S.J.
  TITLE     Changes in integrity and association of eukaryotic protein
            synthesis initiation factors during apoptosis
  JOURNAL   Eur J Biochem 267 (4), 1083-1091 (2000)
   PUBMED   10672017
REFERENCE   10 (bases 1 to 2479)
  AUTHORS   Block,K.L., Vornlocher,H.P. and Hershey,J.W.
  TITLE     Characterization of cDNAs encoding the p44 and p35 subunits of
            human translation initiation factor eIF3
  JOURNAL   J Biol Chem 273 (48), 31901-31908 (1998)
   PUBMED   9822659
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Francesco Amaldi. The reference sequence was
            derived from BC002719.2, AC009996.7 and BU621047.1.
            
            On Nov 22, 2018 this sequence version replaced NM_003758.3.
            
            Summary: This gene encodes a core subunit of the eukaryotic
            initiation factor 3 complex, which participates in the initiation
            of translation by aiding in the recruitment of protein and mRNA
            components to the 40S ribosome. There are pseudogenes for this gene
            on chromosomes 1, 3, and 9. Alternative splicing results in
            multiple transcript variants encoding different isoforms. [provided
            by RefSeq, Sep 2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.707346.1,
                                           SRR1660803.116016.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2162823, SAMEA2163105
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261868.10/ ENSP00000261868.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1765              BC002719.2         13-1777
            1766-1796           AC009996.7         47916-47946
            1797-2479           BU621047.1         17-699              c
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.1"
     gene            1..2479
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /note="eukaryotic translation initiation factor 3 subunit
                     J"
                     /db_xref="GeneID:8669"
                     /db_xref="HGNC:HGNC:3270"
                     /db_xref="MIM:603910"
     exon            1..91
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..825
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     eukaryotic translation initiation factor 3, subunit 1
                     (alpha, 35kD)"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 3
                     subunit J isoform 1"
                     /protein_id="NP_003749.2"
                     /db_xref="CCDS:CCDS10111.1"
                     /db_xref="GeneID:8669"
                     /db_xref="HGNC:HGNC:3270"
                     /db_xref="MIM:603910"
                     /translation="MAAAAAAAGDSDSWDADAFSVEDPVRKVGGGGTAGGDRWEGEDE
                     DEDVKDNWDDDDDEKKEEAEVKPEVKISEKKKIAEKIKEKERQQKKRQEEIKKRLEEP
                     EEPKVLTPEEQLADKLRLKKLQEESDLELAKETFGVNNAVYGIDAMNPSSRDDFTEFG
                     KLLKDKITQYEKSLYYASFLEVLVRDVCISLEIDDLKKITNSLTVLCSEKQKQEKQSK
                     AKKKKKGVVPGGGLKATMKDDLADYGGYDGGYVQDYEDFM"
     exon            92..195
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /inference="alignment:Splign:2.1.0"
     exon            196..250
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /inference="alignment:Splign:2.1.0"
     exon            251..342
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /inference="alignment:Splign:2.1.0"
     exon            343..457
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /inference="alignment:Splign:2.1.0"
     exon            458..619
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /inference="alignment:Splign:2.1.0"
     exon            620..693
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /inference="alignment:Splign:2.1.0"
     exon            694..2479
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1738..1743
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /note="hexamer: AATAAA"
     polyA_site      1765
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /note="major polyA site"
     regulatory      1853..1858
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /note="hexamer: AATAAA"
     polyA_site      1884
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
     regulatory      2457..2462
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /note="hexamer: ATTAAA"
     polyA_site      2479
                     /gene="EIF3J"
                     /gene_synonym="eIF3-alpha; eIF3-p35; EIF3S1"
                     /note="major polyA site"
ORIGIN      
        1 aactcctcgc tagctctccc tctcacacac gctcacaccc ggctcgagat ggcggcggcg
       61 gcggcggcgg cgggggactc ggactcctgg gacgccgacg ctttctccgt ggaagaccca
      121 gtgcggaagg tggggggcgg cggcactgcc ggcggggacc gctgggaagg cgaggacgag
      181 gacgaggacg tcaaggataa ctgggatgac gatgatgatg aaaaaaaaga ggaagcagaa
      241 gtaaaaccag aggtaaaaat ttcagaaaag aaaaaaatag cagagaagat aaaagagaaa
      301 gaacggcaac agaagaaaag gcaagaagaa attaaaaaga ggttagaaga acccgaagaa
      361 cctaaagtgc taacaccaga agaacaatta gcagataaac tgcggctaaa gaaattacag
      421 gaagagtcag acctcgaatt agcaaaggaa acttttggtg ttaataatgc agtttatgga
      481 atagatgcta tgaacccatc ttcaagagat gactttacag agtttggaaa gttactaaaa
      541 gataaaatta cacaatatga aaagtcacta tattatgcca gttttttgga agtcttagtt
      601 cgagatgtgt gtatttcatt ggaaattgat gacttgaaaa aaattaccaa ttcactgact
      661 gtgctttgca gtgaaaaaca gaagcaagaa aagcaaagca aagccaaaaa gaagaagaaa
      721 ggtgtggttc ctggaggggg attaaaagcc accatgaaag atgatctggc agattatggt
      781 ggttatgatg gaggatatgt acaagactat gaagacttca tgtgacattt tatcttttct
      841 tggtgtcatc tttatgttgc ccacaatccc ttgaacatgt agcacaactt cctttccttt
      901 cagttctgcc aaatgctaca atcagaagtg cagtatcttt tgtgctggtt atttaacccc
      961 ttgacactta ggtgctaatg tgcaaatgag ggaacttgga tcttgctgcc aaggggttaa
     1021 aattgggaac ctaagttgct actaaatcat agttcaaaac ctaataatgt tgtcgttgtt
     1081 gctatctgat ttcatagcag cagtcactaa attggaaaca aaaggttgca acgtgacaaa
     1141 aaaaattgtg tagtatttac cagcaccatt cagtaataca gccttaacca tacctccttg
     1201 aactacttca taacttgtca agaaaagcag tttgcagcaa gggcatgtgg tgtgcaccta
     1261 gtattaaaat tgctttgtct taaaattgaa catgaggata ttaaaaatac attgtgaaga
     1321 agactgctta tctcagagtg aagatactgc ggctgaaaag cactagtttg atataaaatt
     1381 aaaatgacca aaaccctcca actttgaagc taaagaaggt aaacctttcc attattgcat
     1441 tacatgttgt ggaatctctc gagtgcaaag actgtctagt tatttatcag gctatttcta
     1501 ctgatgaact gcttcaggtg ggggagggaa acttattttt atttgcctga tttaagtgtc
     1561 tgagaaacaa atctttgttc tcttaggctg caatggaaca actttaccag ggttttggca
     1621 tttcctttcc tttcctttat aaaacatgct cagcaaactg caccagttaa ctacagtttg
     1681 gtaaattgtt atgttaacaa ttatgacatc tgcaatgttt tataaagcaa ctaatttaat
     1741 aaaatcactg ttgtgaggac ttaaattttg tgttacctcc caagagatac tttttgagag
     1801 tatagaacac agctcttggg agtacagttc tctacgttct ctactaaatc ttaataaatg
     1861 cttgacatag ttacagcttt aaaacatgag tgatttgcca ggtccttatg ttgtcaccat
     1921 agagcaacaa aggtataggg ctgccttcct cttatttatt tggggacatt attttgttat
     1981 ttagatacca aggcctaatt aattaagtac ctataagaac tatttatttg gagtaactga
     2041 gcctgtaact caggtttatg gctgttaagt atagattggg gaatctttat tatgtcttct
     2101 cctaagcagt ttaaccaaat gtgtggttag tgttttttta ttcccctaag acagaaagaa
     2161 caaaaaatgt tttaaatttc tcttatatag gaaataatag gaacgtcaaa gctctgtata
     2221 cctactaagt ggaaaacaag accatcatct aagtgatttg agaaattaac aaaagtagtg
     2281 actacacagc aataattaca gtaaattaaa taaagattcc tttaaggcag acaagggcta
     2341 agatttcctt agcagtaata atgacataca ctgaattgaa aatctatttt attacagaaa
     2401 gatcagtttc taacaaatga aaatgtatca cctgttcctt aactgtgtaa ataataatta
     2461 aatttctttg aaactggaa
//
