LOCUS       NM_003755               1103 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens eukaryotic translation initiation factor 3 subunit G
            (EIF3G), mRNA.
ACCESSION   NM_003755
VERSION     NM_003755.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Chang,J., Shin,M.K., Park,J., Hwang,H.J., Locker,N., Ahn,J.,
            Kim,D., Baek,D., Park,Y., Lee,Y., Boo,S.H., Kim,H.I. and Kim,Y.K.
  TITLE     An interaction between eIF4A3 and eIF3g drives the internal
            initiation of translation
  JOURNAL   Nucleic Acids Res 51 (20), 10950-10969 (2023)
   PUBMED   37811880
  REMARK    GeneRIF: An interaction between eIF4A3 and eIF3g drives the
            internal initiation of translation.
REFERENCE   3  (bases 1 to 1103)
  AUTHORS   Abaeva,I.S., Arhab,Y., Miscicka,A., Hellen,C.U.T. and Pestova,T.V.
  TITLE     In vitro reconstitution of SARS-CoV-2 Nsp1-induced mRNA cleavage
            reveals the key roles of the N-terminal domain of Nsp1 and the RRM
            domain of eIF3g
  JOURNAL   Genes Dev 37 (17-18), 844-860 (2023)
   PUBMED   37821106
  REMARK    GeneRIF: In vitro reconstitution of SARS-CoV-2 Nsp1-induced mRNA
            cleavage reveals the key roles of the N-terminal domain of Nsp1 and
            the RRM domain of eIF3g.
REFERENCE   4  (bases 1 to 1103)
  AUTHORS   Pintacuda,G., Hsu,Y.H., Tsafou,K., Li,K.W., Martin,J.M.,
            Riseman,J., Biagini,J.C., Ching,J.K.T., Mena,D.,
            Gonzalez-Lozano,M.A., Egri,S.B., Jaffe,J., Smit,A.B., Fornelos,N.,
            Eggan,K.C. and Lage,K.
  TITLE     Protein interaction studies in human induced neurons indicate
            convergent biology underlying autism spectrum disorders
  JOURNAL   Cell Genom 3 (3), 100250 (2023)
   PUBMED   36950384
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1103)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 1103)
  AUTHORS   Hou,C.L., Tang,Cj., Roffler,S.R. and Tang,T.K.
  TITLE     Protein 4.1R binding to eIF3-p44 suggests an interaction between
            the cytoskeletal network and the translation apparatus
  JOURNAL   Blood 96 (2), 747-753 (2000)
   PUBMED   10887144
REFERENCE   7  (bases 1 to 1103)
  AUTHORS   Bandyopadhyay,A. and Maitra,U.
  TITLE     Cloning and characterization of the p42 subunit of mammalian
            translation initiation factor 3 (eIF3): demonstration that eIF3
            interacts with eIF5 in mammalian cells
  JOURNAL   Nucleic Acids Res 27 (5), 1331-1337 (1999)
   PUBMED   9973622
REFERENCE   8  (bases 1 to 1103)
  AUTHORS   Block,K.L., Vornlocher,H.P. and Hershey,J.W.
  TITLE     Characterization of cDNAs encoding the p44 and p35 subunits of
            human translation initiation factor eIF3
  JOURNAL   J Biol Chem 273 (48), 31901-31908 (1998)
   PUBMED   9822659
REFERENCE   9  (bases 1 to 1103)
  AUTHORS   Methot,N., Rom,E., Olsen,H. and Sonenberg,N.
  TITLE     The human homologue of the yeast Prt1 protein is an integral part
            of the eukaryotic initiation factor 3 complex and interacts with
            p170
  JOURNAL   J Biol Chem 272 (2), 1110-1116 (1997)
   PUBMED   8995410
REFERENCE   10 (bases 1 to 1103)
  AUTHORS   Asano,K., Kinzy,T.G., Merrick,W.C. and Hershey,J.W.
  TITLE     Conservation and diversity of eukaryotic translation initiation
            factor eIF3
  JOURNAL   J Biol Chem 272 (2), 1101-1109 (1997)
   PUBMED   8995409
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Francesco Amaldi. The reference sequence was
            derived from BC000733.2 and AA588881.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003755.4.
            
            Summary: This gene encodes a core subunit of the eukaryotic
            translation initiation factor 3 (eIF3) complex, which is required
            for initiation of protein translation. An N-terminal caspase
            cleavage product of the encoded protein may stimulate degradation
            of DNA. A mutation in this gene is associated with narcolepsy.
            [provided by RefSeq, Jul 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF094850.1, BC000733.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467144 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000253108.9/ ENSP00000253108.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1099              BC000733.2         3-1101
            1100-1103           AA588881.1         31-34               c
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.2"
     gene            1..1103
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /note="eukaryotic translation initiation factor 3 subunit
                     G"
                     /db_xref="GeneID:8666"
                     /db_xref="HGNC:HGNC:3274"
                     /db_xref="MIM:603913"
     exon            1..60
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..1003
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /note="eukaryotic translation initiation factor 3 subunit
                     p42; eukaryotic translation initiation factor 3, subunit 4
                     delta, 44kDa; eukaryotic translation initiation factor 3
                     RNA-binding subunit"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 3
                     subunit G"
                     /protein_id="NP_003746.2"
                     /db_xref="CCDS:CCDS12227.1"
                     /db_xref="GeneID:8666"
                     /db_xref="HGNC:HGNC:3274"
                     /db_xref="MIM:603913"
                     /translation="MPTGDFDSKPSWADQVEEEGEDDKCVTSELLKGIPLATGDTSPE
                     PELLPGAPLPPPKEVINGNIKTVTEYKIDEDGKKFKIVRTFRIETRKASKAVARRKNW
                     KKFGNSEFDPPGPNVATTTVSDDVSMTFITSKEDLNCQEEEDPMNKLKGQKIVSCRIC
                     KGDHWTTRCPYKDTLGPMQKELAEQLGLSTGEKEKLPGELEPVQATQNKTGKYVPPSL
                     RDGASRRGESMQPNRRADDNATIRVTNLSEDTRETDLQELFRPFGSISRIYLAKDKTT
                     GQSKGFAFISFHRREDAARAIAGVSGFGYDHLILNVEWAKPSTN"
     exon            61..107
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /inference="alignment:Splign:2.1.0"
     exon            108..191
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /inference="alignment:Splign:2.1.0"
     exon            192..280
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /inference="alignment:Splign:2.1.0"
     exon            281..340
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /inference="alignment:Splign:2.1.0"
     exon            341..445
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /inference="alignment:Splign:2.1.0"
     exon            446..635
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /inference="alignment:Splign:2.1.0"
     exon            636..743
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /inference="alignment:Splign:2.1.0"
     exon            744..880
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /inference="alignment:Splign:2.1.0"
     exon            881..987
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1103
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1080..1085
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /note="hexamer: AATAAA"
     polyA_site      1103
                     /gene="EIF3G"
                     /gene_synonym="eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4"
                     /note="major polyA site"
ORIGIN      
        1 ctctggccgg gcttgggctg cgtggagaat actttttgcg atgcctactg gagactttga
       61 ttcgaagccc agttgggccg accaggtgga ggaggagggg gaggacgaca aatgtgtcac
      121 cagcgagctc ctcaagggga tccctctggc cacaggtgac accagcccag agccagagct
      181 actgccggga gctccactgc cgcctcccaa ggaggtcatc aacggaaaca taaagacagt
      241 gacagagtac aagatagatg aggatggcaa gaagttcaag attgtccgca ccttcaggat
      301 tgagacccgg aaggcttcaa aggctgtcgc aaggaggaag aactggaaga agttcgggaa
      361 ctcagagttt gacccccccg gacccaatgt ggccaccacc actgtcagtg acgatgtctc
      421 tatgacgttc atcaccagca aagaggacct gaactgccag gaggaggagg accctatgaa
      481 caaactcaag ggccagaaga tcgtgtcctg ccgcatctgc aagggcgacc actggaccac
      541 ccgctgcccc tacaaggata cgctggggcc catgcagaag gagctggccg agcagctggg
      601 cctgtctact ggcgagaagg agaagctgcc gggagagcta gagccggtgc aggccacgca
      661 gaacaagaca gggaagtatg tgccgccgag cctgcgcgac ggggccagcc gccgcgggga
      721 gtccatgcag cccaaccgca gagccgacga caacgccacc atccgtgtca ccaacttgtc
      781 agaggacacg cgtgagaccg acctgcagga gctcttccgg cctttcggct ccatctcccg
      841 catctacctg gctaaggaca agaccactgg ccaatccaag ggctttgcct tcatcagctt
      901 ccaccgccgc gaggatgctg cgcgtgccat tgccggggtg tccggctttg gctacgacca
      961 cctcatcctc aacgtcgagt gggccaagcc gtccaccaac taagccagct gccactgtgt
     1021 actcggtccg ggacccttgg cgacagaaga cagcctccga gagcgcgggc tccaagggca
     1081 ataaagcagc tccactctct cta
//
