LOCUS       NM_003908               2556 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens eukaryotic translation initiation factor 2 subunit
            beta (EIF2S2), transcript variant 1, mRNA.
ACCESSION   NM_003908
VERSION     NM_003908.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Fatalska,A., Hodgson,G., Freund,S.M.V., Maslen,S.L., Morgan,T.,
            Thorkelsson,S.R., van Slegtenhorst,M., Lorenz,S., Andreeva,A.,
            Kaat,L.D. and Bertolotti,A.
  TITLE     Recruitment of trimeric eIF2 by phosphatase non-catalytic subunit
            PPP1R15B
  JOURNAL   Mol Cell 84 (3), 506-521 (2024)
   PUBMED   38159565
  REMARK    GeneRIF: Recruitment of trimeric eIF2 by phosphatase non-catalytic
            subunit PPP1R15B.
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 2556)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2556)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 2556)
  AUTHORS   Zhang,J., Li,S., Zhang,L., Xu,J., Song,M., Shao,T., Huang,Z. and
            Li,Y.
  TITLE     RBP EIF2S2 Promotes Tumorigenesis and Progression by Regulating
            MYC-Mediated Inhibition via FHIT-Related Enhancers
  JOURNAL   Mol Ther 28 (4), 1105-1118 (2020)
   PUBMED   32059763
  REMARK    GeneRIF: RBP EIF2S2 Promotes Tumorigenesis and Progression by
            Regulating MYC-Mediated Inhibition via FHIT-Related Enhancers.
            Erratum:[Mol Ther. 2021 Feb 3;29(2):886. doi:
            10.1016/j.ymthe.2020.12.020.. PMID: 33341125]
REFERENCE   6  (bases 1 to 2556)
  AUTHORS   Das,S., Maiti,T., Das,K. and Maitra,U.
  TITLE     Specific interaction of eukaryotic translation initiation factor 5
            (eIF5) with the beta-subunit of eIF2
  JOURNAL   J Biol Chem 272 (50), 31712-31718 (1997)
   PUBMED   9395514
REFERENCE   7  (bases 1 to 2556)
  AUTHORS   Brand,S.R., Kobayashi,R. and Mathews,M.B.
  TITLE     The Tat protein of human immunodeficiency virus type 1 is a
            substrate and inhibitor of the interferon-induced, virally
            activated protein kinase, PKR
  JOURNAL   J Biol Chem 272 (13), 8388-8395 (1997)
   PUBMED   9079663
REFERENCE   8  (bases 1 to 2556)
  AUTHORS   Welsh,G.I., Price,N.T., Bladergroen,B.A., Bloomberg,G. and
            Proud,C.G.
  TITLE     Identification of novel phosphorylation sites in the beta-subunit
            of translation initiation factor eIF-2
  JOURNAL   Biochem Biophys Res Commun 201 (3), 1279-1288 (1994)
   PUBMED   8024572
REFERENCE   9  (bases 1 to 2556)
  AUTHORS   Pathak,V.K., Nielsen,P.J., Trachsel,H. and Hershey,J.W.
  TITLE     Structure of the beta subunit of translational initiation factor
            eIF-2
  JOURNAL   Cell 54 (5), 633-639 (1988)
   PUBMED   3044606
REFERENCE   10 (bases 1 to 2556)
  AUTHORS   Safer,B., Adams,S.L., Anderson,W.F. and Merrick,W.C.
  TITLE     Binding of MET-TRNAf and GTP to homogeneous initiation factor MP
  JOURNAL   J Biol Chem 250 (23), 9076-9082 (1975)
   PUBMED   1104615
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Francesco Amaldi. The reference sequence was
            derived from BC000461.2, AL031668.23 and CA436754.1.
            
            On Nov 22, 2018 this sequence version replaced NM_003908.4.
            
            Summary: Eukaryotic translation initiation factor 2 (EIF-2)
            functions in the early steps of protein synthesis by forming a
            ternary complex with GTP and initiator tRNA and binding to a 40S
            ribosomal subunit. EIF-2 is composed of three subunits, alpha,
            beta, and gamma, with the protein encoded by this gene representing
            the beta subunit. The beta subunit catalyzes the exchange of GDP
            for GTP, which recycles the EIF-2 complex for another round of
            initiation. Multiple transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Oct
            2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.96523.1,
                                           SRR1660803.162664.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374980.3/ ENSP00000364119.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1243              BC000461.2         4-1246
            1244-2519           AL031668.23        72132-73407         c
            2520-2556           CA436754.1         29-65               c
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.22"
     gene            1..2556
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /note="eukaryotic translation initiation factor 2 subunit
                     beta"
                     /db_xref="GeneID:8894"
                     /db_xref="HGNC:HGNC:3266"
                     /db_xref="MIM:603908"
     exon            1..148
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /inference="alignment:Splign:2.1.0"
     CDS             134..1135
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /note="isoform 1 is encoded by transcript variant 1;
                     eukaryotic translation initiation factor 2 subunit 2;
                     protein phosphatase 1, regulatory subunit 67; eukaryotic
                     translation initiation factor 2, subunit 2 beta, 38kDa;
                     eIF2-beta"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 2
                     subunit 2 isoform 1"
                     /protein_id="NP_003899.2"
                     /db_xref="CCDS:CCDS13231.1"
                     /db_xref="GeneID:8894"
                     /db_xref="HGNC:HGNC:3266"
                     /db_xref="MIM:603908"
                     /translation="MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEV
                     EPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLK
                     IESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFS
                     NQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTS
                     FVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIK
                     EYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN"
     exon            149..326
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /inference="alignment:Splign:2.1.0"
     exon            327..430
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /inference="alignment:Splign:2.1.0"
     exon            431..566
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /inference="alignment:Splign:2.1.0"
     exon            567..667
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /inference="alignment:Splign:2.1.0"
     exon            668..816
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /inference="alignment:Splign:2.1.0"
     exon            817..873
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /inference="alignment:Splign:2.1.0"
     exon            874..959
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /inference="alignment:Splign:2.1.0"
     exon            960..2556
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1404..1409
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /note="hexamer: ATTAAA"
     regulatory      1413..1418
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /note="hexamer: ATTATA"
     polyA_site      1425
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
     polyA_site      1434
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /note="major polyA site"
     regulatory      2526..2531
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
                     /note="hexamer: AATAAA"
     polyA_site      2556
                     /gene="EIF2S2"
                     /gene_synonym="eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67"
ORIGIN      
        1 gtcgtttcct ttcgctgatg caagagccta gtgcggtggt gggagaggta tcggcagggg
       61 cagcgctgcc gccggggcct ggggctgacc cgtctgactt cccgtccgtg ccgagcccac
      121 tcgagccgca gccatgtctg gggacgagat gatttttgat cctactatga gcaagaagaa
      181 aaagaagaag aagaagcctt ttatgttaga tgaggaaggg gatacccaaa cagaggaaac
      241 ccagccttca gaaacaaaag aagtggagcc agagccaact gaggacaagg atttggaagc
      301 tgatgaagag gacactagga aaaaagatgc ttctgatgat ctagatgact tgaacttctt
      361 taatcaaaag aaaaagaaga aaaaaactaa aaagatattt gatattgatg aagctgaaga
      421 aggtgtaaag gatcttaaga ttgaaagtga tgttcaagaa ccaactgaac cagaggatga
      481 ccttgacatt atgcttggca ataaaaagaa gaaaaagaag aatgttaagt tcccagatga
      541 ggatgaaata ctagagaaag atgaagctct agaagatgaa gacaacaaaa aagatgatgg
      601 tatctcattc agtaatcaga caggccctgc ttgggcaggc tcagaaagag actacacata
      661 cgaggagctg ctgaatcgag tgttcaacat catgagggaa aagaatccag atatggttgc
      721 tggggagaaa aggaaatttg tcatgaaacc tccacaagtc gtccgagtag gaaccaagaa
      781 aacttctttt gtcaacttta cagatatctg taaactatta catcgtcagc ccaaacatct
      841 ccttgcattt ttgttggctg aattgggtac aagtggttct atagatggta ataaccaact
      901 tgtaatcaaa ggaagattcc aacagaaaca gatagaaaat gtcttgagaa gatatatcaa
      961 ggaatatgtc acttgtcaca catgccgatc accggacaca atcctgcaga aggacacacg
     1021 actctatttc ctacagtgcg aaacttgtca ttctagatgt tctgttgcca gtatcaaaac
     1081 cggcttccag gctgtcacgg gcaagcgagc acagctccgt gccaaagcta actaatttgc
     1141 taatcactga ttttgcaaag cttgttgtgg agatgtggct ggacaggttt gccatcagag
     1201 tggatatacc gttgtattaa aaacaagata aaaaagctgc caagattttt ggcgagtggt
     1261 tggtctgaag tccttgcaag acgctgatgc tcaagctgtt gacatactca ttgcctactt
     1321 taacacctgt cagagaaacg tgatatgggg taaggaggtg cttttttaaa atcgttcata
     1381 gacttctgta aaatgcaaga taaattaaag ttattataac agtgattctt tcaatttggt
     1441 ttgtccttca gttttctttt ctataaatgt gcttggtgaa atcagcaaga aaccaaccat
     1501 ataaaacagt gtgtgccggg ctctgactga gaaactgaac aatggcaggc gcttcatctg
     1561 ggcagccatt tttcttctct gcttctcttg agatacatag ttaaagtaga cacggcctcc
     1621 cacgcgagtg tggtgggacc ttgagactta ggaggattgc ctgttttcag tgtccaggga
     1681 atgactccat agatttgaat tcttttccgt atgtggggat ggttgctact acctcaccgc
     1741 caagtcctcc cccagctgtt accacatgag ggaatacaag gtgctaggta ccctccttcc
     1801 tcgttggcca ctaagaggtc ttagaaacat agcctgtaaa tgtgtcttgt aactggcaca
     1861 ctaaaaaaga tcctagttga gcatctctct tggtgaggaa gcgatgtgtg tgcggttcag
     1921 gaaactcctc caagaggagg aggagtccaa acagggatgc cagaggaagc tttccgtttt
     1981 cctagccaac ttgtgttctg ggtttgaaaa agacccacca caaatgcttt ttctattttc
     2041 tgatttaaaa gtcgcttttg aatatgacca tgagaaacca agaaatgctg cttgtgtggt
     2101 gctctgcttc ctgaggattt ggctggaagg ggattctccg ctggcacatg ggagaaggcc
     2161 atcttctgtg tcggtgctgt gatctgcctt gttctcactt gctgaggata agggcataca
     2221 cgctgctcct cgctttcttg ttgcccgact tcgtactaag cagctcagga gcagtcactc
     2281 agacccaaat gtcttgactg tgttgttttt aatcactgtg acccttaaag tacaggccaa
     2341 gtgttgtcaa acaccttggc taaaacagtg gagggtgatg ggtaaagcag tagagggccc
     2401 tcaacccaca cactggctga aactgccacc aactgccacg atgaacccaa ctgctgttta
     2461 tgcccccatt ttcctttttt tgtatctaca cccacacgat tcccaatgtt ggatatttct
     2521 acatgaataa agcaaggatc agtgcctctt atgtaa
//
