LOCUS       NM_001013703            5538 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens eukaryotic translation initiation factor 2 alpha
            kinase 4 (EIF2AK4), mRNA.
ACCESSION   NM_001013703 XM_496066
VERSION     NM_001013703.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Zhu,M.M., Dai,J., Dai,Z., Peng,Y. and Zhao,Y.Y.
  TITLE     GCN2 kinase activation mediates pulmonary vascular remodeling and
            pulmonary arterial hypertension
  JOURNAL   JCI Insight 9 (20), e177926 (2024)
   PUBMED   39316438
  REMARK    GeneRIF: GCN2 kinase activation mediates pulmonary vascular
            remodeling and pulmonary arterial hypertension.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Piecyk,M., Triki,M., Laval,P.A., Duret,C., Fauvre,J.,
            Cussonneau,L., Machon,C., Guitton,J., Rama,N., Gibert,B., Ichim,G.,
            Catez,F., Bourdelais,F., Durand,S., Diaz,J.J., Coste,I., Renno,T.,
            Manie,S.N., Aznar,N., Ansieau,S., Ferraro-Peyret,C. and
            Chaveroux,C.
  TITLE     The stress sensor GCN2 differentially controls ribosome biogenesis
            in colon cancer according to the nutritional context
  JOURNAL   Mol Oncol 18 (9), 2111-2135 (2024)
   PUBMED   37452637
  REMARK    GeneRIF: The stress sensor GCN2 differentially controls ribosome
            biogenesis in colon cancer according to the nutritional context.
REFERENCE   3  (bases 1 to 5538)
  AUTHORS   Emanuelli,G., Zhu,J., Li,W., Morrell,N.W. and Marciniak,S.J.
  TITLE     Functional validation of EIF2AK4 (GCN2) missense variants
            associated with pulmonary arterial hypertension
  JOURNAL   Hum Mol Genet 33 (17), 1495-1505 (2024)
   PUBMED   38776952
  REMARK    GeneRIF: Functional validation of EIF2AK4 (GCN2) missense variants
            associated with pulmonary arterial hypertension.
REFERENCE   4  (bases 1 to 5538)
  AUTHORS   Misra,J., Carlson,K.R., Spandau,D.F. and Wek,R.C.
  TITLE     Multiple mechanisms activate GCN2 eIF2 kinase in response to
            diverse stress conditions
  JOURNAL   Nucleic Acids Res 52 (4), 1830-1846 (2024)
   PUBMED   38281137
  REMARK    GeneRIF: Multiple mechanisms activate GCN2 eIF2 kinase in response
            to diverse stress conditions.
REFERENCE   5  (bases 1 to 5538)
  AUTHORS   Hou,Y., Wei,D., Zhang,Z., Lei,T., Li,S., Bao,J., Guo,H., Tan,L.,
            Xie,X., Zhuang,Y., Lu,Z. and Zhao,Y.
  TITLE     Downregulation of nutrition sensor GCN2 in macrophages contributes
            to poor wound healing in diabetes
  JOURNAL   Cell Rep 43 (1), 113658 (2024)
   PUBMED   38175755
  REMARK    GeneRIF: Downregulation of nutrition sensor GCN2 in macrophages
            contributes to poor wound healing in diabetes.
REFERENCE   6  (bases 1 to 5538)
  AUTHORS   Dey,M., Cao,C., Sicheri,F. and Dever,T.E.
  TITLE     Conserved intermolecular salt bridge required for activation of
            protein kinases PKR, GCN2, and PERK
  JOURNAL   J Biol Chem 282 (9), 6653-6660 (2007)
   PUBMED   17202131
REFERENCE   7  (bases 1 to 5538)
  AUTHORS   Berlanga,J.J., Ventoso,I., Harding,H.P., Deng,J., Ron,D.,
            Sonenberg,N., Carrasco,L. and de Haro,C.
  TITLE     Antiviral effect of the mammalian translation initiation factor
            2alpha kinase GCN2 against RNA viruses
  JOURNAL   EMBO J 25 (8), 1730-1740 (2006)
   PUBMED   16601681
REFERENCE   8  (bases 1 to 5538)
  AUTHORS   Costa-Mattioli,M., Gobert,D., Harding,H., Herdy,B., Azzi,M.,
            Bruno,M., Bidinosti,M., Ben Mamou,C., Marcinkiewicz,E., Yoshida,M.,
            Imataka,H., Cuello,A.C., Seidah,N., Sossin,W., Lacaille,J.C.,
            Ron,D., Nader,K. and Sonenberg,N.
  TITLE     Translational control of hippocampal synaptic plasticity and memory
            by the eIF2alpha kinase GCN2
  JOURNAL   Nature 436 (7054), 1166-1173 (2005)
   PUBMED   16121183
REFERENCE   9  (bases 1 to 5538)
  AUTHORS   Zhang,P., McGrath,B.C., Reinert,J., Olsen,D.S., Lei,L., Gill,S.,
            Wek,S.A., Vattem,K.M., Wek,R.C., Kimball,S.R., Jefferson,L.S. and
            Cavener,D.R.
  TITLE     The GCN2 eIF2alpha kinase is required for adaptation to amino acid
            deprivation in mice
  JOURNAL   Mol Cell Biol 22 (19), 6681-6688 (2002)
   PUBMED   12215525
REFERENCE   10 (bases 1 to 5538)
  AUTHORS   Berlanga,J.J., Santoyo,J. and De Haro,C.
  TITLE     Characterization of a mammalian homolog of the GCN2 eukaryotic
            initiation factor 2alpha kinase
  JOURNAL   Eur J Biochem 265 (2), 754-762 (1999)
   PUBMED   10504407
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC012377.5, CN286138.1,
            BC078179.1, AB037759.1, BC014915.1, BC009350.2 and AL157497.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001013703.3.
            
            Summary: This gene encodes a member of a family of kinases that
            phosphorylate the alpha subunit of eukaryotic translation
            initiation factor-2 (EIF2), resulting in the downregulaton of
            protein synthesis. The encoded protein responds to amino acid
            deprivation by binding uncharged transfer RNAs. It may also be
            activated by glucose deprivation and viral infection. Mutations in
            this gene have been found in individuals suffering from autosomal
            recessive pulmonary venoocclusive-disease-2. [provided by RefSeq,
            Mar 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1965299,
                              SAMEA1966682 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000263791.10/ ENSP00000263791.5
            RefSeq Select criteria    :: based on single protein-coding
                                         transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 AC012377.5         173251-173259
            10-179              CN286138.1         1-170
            180-180             AC012377.5         173430-173430
            181-604             BC078179.1         150-573
            605-607             AB037759.1         62-64
            608-1899            BC078179.1         577-1868
            1900-3062           AB037759.1         1357-2519
            3063-4295           BC014915.1         1421-2653
            4296-5443           BC009350.2         1757-2904
            5444-5537           AB037759.1         4901-4994
            5538-5538           AL157497.1         3326-3326
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.1"
     gene            1..5538
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /note="eukaryotic translation initiation factor 2 alpha
                     kinase 4"
                     /db_xref="GeneID:440275"
                     /db_xref="HGNC:HGNC:19687"
                     /db_xref="MIM:609280"
     exon            1..225
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..5031
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /EC_number="2.7.11.1"
                     /note="GCN2 eIF2alpha kinase; GCN2-like protein; general
                     control nonderepressible 2"
                     /codon_start=1
                     /product="eIF-2-alpha kinase GCN2"
                     /protein_id="NP_001013725.2"
                     /db_xref="CCDS:CCDS42016.1"
                     /db_xref="GeneID:440275"
                     /db_xref="HGNC:HGNC:19687"
                     /db_xref="MIM:609280"
                     /translation="MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPD
                     ACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNES
                     VNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQR
                     LLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPG
                     GHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPD
                     QLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKID
                     KCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHLS
                     HSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL
                     ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDL
                     PADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIP
                     SNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPIN
                     PASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAA
                     RGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSASARFPATGPGSSDDEDDDEDE
                     HGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYL
                     YIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFL
                     DSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQG
                     STKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHA
                     KQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTM
                     MAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLL
                     LPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPR
                     KLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTM
                     LLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIE
                     QKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYK
                     VQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAI
                     DKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQ
                     EELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLETELVDHVLQKLRTKV
                     TDERNGREASDNLAVQNLKGSFSNASGLFEIHGATVVPIVSVLAPEKLSASTRRRYET
                     QVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRL
                     PKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF"
     exon            226..338
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            339..441
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            442..594
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            595..675
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            676..824
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            825..940
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1098
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1634
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1741
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..1899
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..2330
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2331..2400
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2401..2484
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2485..2607
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2608..2712
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2713..2767
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2768..2847
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2848..2949
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2950..3003
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3004..3240
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3241..3316
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3317..3438
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3439..3488
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3489..3657
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3658..3774
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3775..3840
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3841..4011
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4012..4146
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4147..4254
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4255..4383
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4384..4470
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4471..4583
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4584..4642
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4643..4740
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4741..4809
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4810..4854
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4855..4973
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4974..5538
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5521..5526
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /note="hexamer: AATAAA"
     polyA_site      5538
                     /gene="EIF2AK4"
                     /gene_synonym="GCN2; PVOD2"
                     /note="major polyA site"
ORIGIN      
        1 agcgcggagc cccgccccgc aggctgccgg gggcccaccg ccgcccaggc aaggccgccc
       61 tgccttgggc gcagcgctgc catggctggg ggccgtgggg cccccgggcg cggccgggac
      121 gagcctccgg agagctaccc gcaacgacag gaccacgagc tacaggccct ggaggccatt
      181 tacggcgcgg acttccaaga cctgcggccg gacgcttgcg gaccggtcaa agagccccct
      241 gaaatcaatt tagttttgta ccctcaaggc ctaactggtg aagaagtata tgtaaaagtg
      301 gatttgaggg ttaaatgccc acctacctat ccagatgtag ttcctgaaat agagttaaaa
      361 aatgccaaag gtctatcaaa tgaaagtgtc aatttgttaa aatctcgcct agaagaactg
      421 gccaagaaac actgtgggga ggtgatgatc tttgaactgg cttaccacgt gcagtcattt
      481 ctcagcgagc ataacaagcc ccctcccaag tcttttcatg aagaaatgct ggaaaggcgg
      541 gctcaggagg agcagcagag gctgttggag gccaagcgga aagaagagca ggagcaacgt
      601 gaaatcctgc atgagattca gagaaggaaa gaagagataa aagaagagaa aaaaaggaaa
      661 gaaatggcta agcaggaacg tttggaaatt gctagtttgt caaaccaaga tcatacctct
      721 aagaaggacc caggaggaca cagaacggct gccattctac atggaggctc tcctgacttt
      781 gtaggaaatg gtaaacatcg ggcaaactcc tcaggaaggt ctaggcgaga acgtcagtat
      841 tctgtatgta atagtgaaga ttctcctggc tcttgtgaaa ttctgtattt caatatgggg
      901 agtcctgatc agctcatggt gcacaaaggg aaatgtattg gcagtgatga acaacttgga
      961 aaattagtct acaatgcttt ggaaacagcc actggtggct ttgtcttgtt gtatgagtgg
     1021 gtccttcagt ggcagaaaaa aatgggtcca ttccttacca gtcaagaaaa agagaagatt
     1081 gataagtgca aaaagcagat tcaaggaaca gaaacagaat tcaactcact ggtaaaattg
     1141 agccatccaa atgtagtacg ctaccttgca atgaatctca aagagcaaga cgactccatc
     1201 gtggtggaca ttttagtgga gcacattagt ggggtctctc ttgctgcaca cctgagccac
     1261 tcaggcccca tccctgtgca tcagcttcgc aggtacacag ctcagctcct gtcaggcctt
     1321 gattatctgc acagcaattc tgtggtgcat aaggtcctga gtgcatctaa tgtcttggtg
     1381 gatgcagaag gcaccgtcaa gattacggac tatagcattt ctaagcgcct cgcagacatt
     1441 tgcaaggagg atgtgtttga gcaaacccga gttcgtttta gtgacaatgc tctgccttat
     1501 aaaacgggga agaaaggaga tgtttggcgt cttggccttc tgctgctgtc cctcagccaa
     1561 ggacaggaat gtggagagta ccctgtgacc atccctagtg acttaccagc tgactttcaa
     1621 gattttctaa agaaatgtgt gtgcttggat gacaaggaaa gatggagtcc ccagcagttg
     1681 ttgaaacaca gctttataaa tccccagcca aaaatgcctc tagtggaaca aagtcctgaa
     1741 gattctgaag gacaagatta tgttgagact gttattccta gcaaccggct acccagtgct
     1801 gccttcttta gtgagacaca gagacagttt tcccgatact tcattgagtt tgaagaatta
     1861 caacttcttg gtaaaggagc ttttggagct gtcatcaagg tgcagaacaa gttggacggc
     1921 tgctgctacg cagtgaagcg catccccatc aacccggcca gccggcagtt ccgcaggatc
     1981 aagggcgaag tgacactgct gtcacggctg caccatgaga acattgtgcg ctactacaac
     2041 gcctggatcg agcggcacga gcggccggcg ggaccgggga cgccgccccc ggactccggg
     2101 cccctggcca aggatgaccg agctgcacgc gggcagccgg cgagcgacac agacggcctg
     2161 gacagcgtag aggccgccgc gccgccaccc atcctcagca gctcggtgga gtggagcact
     2221 tcgggcgagc gctcggccag tgcccgtttc cccgccaccg gcccgggctc cagcgatgac
     2281 gaggacgacg acgaggacga gcacggtggc gtcttctccc agtccttcct gcctgcttca
     2341 gattctgaaa gtgatattat ctttgacaat gaagatgaga acagtaaaag tcagaatcag
     2401 gatgaagatt gcaatgaaaa gaatggctgc catgaaagtg agccatcagt gacgactgag
     2461 gctgtgcact acctatacat ccagatggag tactgtgaga agagcacttt acgagacacc
     2521 attgaccagg gactgtatcg agacaccgtc agactctgga ggctttttcg agagattctg
     2581 gatggattag cttatatcca tgagaaagga atgattcacc gggatttgaa gcctgtcaac
     2641 atttttttgg attctgatga ccatgtgaaa ataggtgatt ttggtttggc gacagaccat
     2701 ctagcctttt ctgctgacag caaacaagac gatcagacag gagacttgat taagtcagac
     2761 ccttcaggtc acttaactgg gatggttggc actgctctct atgtaagccc agaggtccaa
     2821 ggaagcacca aatctgcata caaccagaaa gtggatctct tcagcctggg aattatcttc
     2881 tttgagatgt cctatcaccc catggtcacg gcttcagaaa ggatctttgt tctcaaccaa
     2941 ctcagagatc ccacttcgcc taagtttcca gaagactttg acgatggaga gcatgcaaag
     3001 cagaaatcag tcatctcctg gctgttgaac cacgatccag caaaacggcc cacagccaca
     3061 gaactgctca agagtgagct gctgccccca ccccagatgg aggagtcaga gctgcatgaa
     3121 gtgctgcacc acacgctgac caacgtggat gggaaggcct accgcaccat gatggcccag
     3181 atcttctcgc agcgcatctc ccctgccatc gattacacct atgacagcga catactgaag
     3241 ggcaacttct caatccgtac agccaagatg cagcagcatg tgtgtgaaac catcatccgc
     3301 atctttaaaa gacatggagc tgttcagttg tgtactccac tactgcttcc ccgaaacaga
     3361 caaatatatg agcacaacga agctgcccta ttcatggacc acagcgggat gctggtgatg
     3421 cttccttttg acctgcggat cccttttgca agatatgtgg caagaaataa tatattgaat
     3481 ttaaaacgat actgcataga acgtgtgttc aggccgcgca agttagatcg atttcatccc
     3541 aaagaacttc tggagtgtgc atttgatatt gtcacttcta ccaccaacag ctttctgccc
     3601 actgctgaaa ttatctacac tatctatgaa atcatccaag agtttccagc acttcaggaa
     3661 agaaattaca gtatttattt gaaccatacc atgttattga aagcaatact cttacactgt
     3721 gggatcccag aagataaact cagtcaagtc tacattattc tgtatgatgc tgtgacagag
     3781 aagctgacga ggagagaagt ggaagctaaa ttttgtaatc tgtctttgtc ttctaatagt
     3841 ctgtgtcgac tctacaagtt tattgaacag aagggagatt tgcaagatct tatgccaaca
     3901 ataaattcat taataaaaca gaaaacaggt attgcacagt tggtgaagta tggcttaaaa
     3961 gacctagagg aggttgttgg actgttgaag aaactcggca tcaagttaca ggtcttgatc
     4021 aatttgggct tggtttacaa ggtgcagcag cacaatggaa tcatcttcca gtttgtggct
     4081 ttcatcaaac gaaggcaaag ggctgtacct gaaatcctcg cagctggagg cagatatgac
     4141 ctgctgattc cccagtttag agggccacaa gctctggggc cagttcccac tgccattggg
     4201 gtcagcatag ctatagacaa gatatctgct gctgtcctca acatggagga atctgttaca
     4261 ataagctctt gtgacctcct ggttgtaagt gttggccaga tgtctatgtc cagggccatc
     4321 aacctaaccc agaaactctg gacagcaggc atcacagcag aaatcatgta cgactggtca
     4381 cagtcccaag aggaattaca agagtactgc agacatcatg aaatcaccta tgtggccctt
     4441 gtctcggata aagaaggaag ccatgtcaag gttaagtctt tcgagaagga aaggcagaca
     4501 gagaagcgtg tgctggagac tgaacttgtg gaccatgtac tgcagaaact gaggactaaa
     4561 gtcactgatg aaaggaatgg cagagaagct tccgataatc ttgcagtgca aaatctgaag
     4621 gggtcatttt ctaatgcttc aggtttgttt gaaatccatg gagcaacagt ggttcccatt
     4681 gtgagtgtgc tagccccgga gaagctgtca gccagcacta ggaggcgcta tgaaactcag
     4741 gtacaaactc gacttcagac ctcccttgcc aacttacatc agaaaagcag tgaaattgaa
     4801 attctggctg tggatctacc caaagaaaca atattacagt ttttatcatt agagtgggat
     4861 gctgatgaac aggcatttaa cacaactgtg aagcagctgc tgtcacgcct gccaaagcaa
     4921 agatacctca aattagtctg tgatgaaatt tataacatca aagtagaaaa aaaggtgtct
     4981 gtgctatttc tgtacagcta tagagatgac tactacagaa tcttatttta accctaaaga
     5041 actgtcgtta acctcattca aacagacaga ggcttatact ggaataatgg aatgttgtac
     5101 attcatcata atttaaaatt aaattctaag aagaggctgg gtgcagtggc tcacaccttt
     5161 aatcccagca ctttgggaag ccaaggcagg aagactgctt gaaaccagga gtttgagacc
     5221 agcctgagca acaaagcaag accccatctc tataaaaact aaaaaaatta gttgggcatg
     5281 gtggcacatg cctgtagtcc cagctactcc agaggctgag atggatcatc tgagcctcag
     5341 gaggttgagg ctgcagtgag ctgtgactgc gccactgcac tccagtctgg gacaacagag
     5401 caagaccctg tcttaaaaaa aaaaagaaaa aaaaaatttt tttctaagaa gctgtcctac
     5461 aaagttgagc tttgttagtt tttcatgtgt aatatattat aaatttatct tttgggatat
     5521 aataaatgct ttcatata
//
