LOCUS       NM_032025               3879 bp    mRNA    linear   PRI 27-JUL-2024
DEFINITION  Homo sapiens eukaryotic translation initiation factor 2A (EIF2A),
            transcript variant 1, mRNA.
ACCESSION   NM_032025
VERSION     NM_032025.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Grove,D.J., Russell,P.J. and Kearse,M.G.
  TITLE     To initiate or not to initiate: A critical assessment of eIF2A,
            eIF2D, and MCT-1.DENR to deliver initiator tRNA to ribosomes
  JOURNAL   Wiley Interdiscip Rev RNA 15 (2), e1833 (2024)
   PUBMED   38433101
  REMARK    GeneRIF: To initiate or not to initiate: A critical assessment of
            eIF2A, eIF2D, and MCT-1.DENR to deliver initiator tRNA to
            ribosomes.
            Review article
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Grove,D.J., Levine,D.J. and Kearse,M.G.
  TITLE     Increased levels of eIF2A inhibit translation by sequestering 40S
            ribosomal subunits
  JOURNAL   Nucleic Acids Res 51 (18), 9983-10000 (2023)
   PUBMED   37602404
  REMARK    GeneRIF: Increased levels of eIF2A inhibit translation by
            sequestering 40S ribosomal subunits.
REFERENCE   3  (bases 1 to 3879)
  AUTHORS   Wei,L.H., Sun,Y. and Guo,J.U.
  TITLE     Genome-wide CRISPR screens identify noncanonical translation factor
            eIF2A as an enhancer of SARS-CoV-2 programmed -1 ribosomal
            frameshifting
  JOURNAL   Cell Rep 42 (8), 112987 (2023)
   PUBMED   37581984
  REMARK    GeneRIF: Genome-wide CRISPR screens identify noncanonical
            translation factor eIF2A as an enhancer of SARS-CoV-2 programmed -1
            ribosomal frameshifting.
REFERENCE   4  (bases 1 to 3879)
  AUTHORS   Waisner,H., Grieshaber,B., Saud,R., Henke,W., Stephens,E.B. and
            Kalamvoki,M.
  TITLE     SARS-CoV-2 Harnesses Host Translational Shutoff and Autophagy To
            Optimize Virus Yields: the Role of the Envelope (E) Protein
  JOURNAL   Microbiol Spectr 11 (1), e0370722 (2023)
   PUBMED   36622177
REFERENCE   5  (bases 1 to 3879)
  AUTHORS   Kim,H., Aponte-Diaz,D., Sotoudegan,M.S., Shengjuler,D., Arnold,J.J.
            and Cameron,C.E.
  TITLE     The enterovirus genome can be translated in an IRES-independent
            manner that requires the initiation factors eIF2A/eIF2D
  JOURNAL   PLoS Biol 21 (1), e3001693 (2023)
   PUBMED   36689548
  REMARK    GeneRIF: The enterovirus genome can be translated in an
            IRES-independent manner that requires the initiation factors
            eIF2A/eIF2D.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3879)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 3879)
  AUTHORS   Komar,A.A., Gross,S.R., Barth-Baus,D., Strachan,R., Hensold,J.O.,
            Goss Kinzy,T. and Merrick,W.C.
  TITLE     Novel characteristics of the biological properties of the yeast
            Saccharomyces cerevisiae eukaryotic initiation factor 2A
  JOURNAL   J Biol Chem 280 (16), 15601-15611 (2005)
   PUBMED   15718232
REFERENCE   8  (bases 1 to 3879)
  AUTHORS   Zoll,W.L., Horton,L.E., Komar,A.A., Hensold,J.O. and Merrick,W.C.
  TITLE     Characterization of mammalian eIF2A and identification of the yeast
            homolog
  JOURNAL   J Biol Chem 277 (40), 37079-37087 (2002)
   PUBMED   12133843
REFERENCE   9  (bases 1 to 3879)
  AUTHORS   Sudhakar,A., Krishnamoorthy,T., Jain,A., Chatterjee,U.,
            Hasnain,S.E., Kaufman,R.J. and Ramaiah,K.V.
  TITLE     Serine 48 in initiation factor 2 alpha (eIF2 alpha) is required for
            high-affinity interaction between eIF2 alpha(P) and eIF2B
  JOURNAL   Biochemistry 38 (46), 15398-15405 (1999)
   PUBMED   10563826
REFERENCE   10 (bases 1 to 3879)
  AUTHORS   Merrick,W.C.
  TITLE     Mechanism and regulation of eukaryotic protein synthesis
  JOURNAL   Microbiol Rev 56 (2), 291-315 (1992)
   PUBMED   1620067
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Francesco Amaldi. The reference sequence was
            derived from AC107426.13.
            
            On Nov 23, 2018 this sequence version replaced NM_032025.4.
            
            Summary: This gene encodes a eukaryotic translation initiation
            factor that catalyzes the formation of puromycin-sensitive 80 S
            preinitiation complexes and the poly(U)-directed synthesis of
            polyphenylalanine at low concentrations of Mg2+. This gene should
            not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha
            subunit of the eIF2 translation initiation complex. Although both
            of these proteins function in binding initiator tRNA to the 40 S
            ribosomal subunit, the encoded protein does so in a codon-dependent
            manner, whereas eIF2 complex requires GTP. Alternative splicing of
            this gene results in multiple transcript variants encoding
            different isoforms. [provided by RefSeq, Jan 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.230470.1,
                                           SRR1803612.176275.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000460851.6/ ENSP00000417229.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                AC107426.13        44060-44103         c
            45-114              AC107426.13        38465-38534         c
            115-189             AC107426.13        32428-32502         c
            190-308             AC107426.13        28230-28348         c
            309-408             AC107426.13        27276-27375         c
            409-491             AC107426.13        26509-26591         c
            492-565             AC107426.13        23124-23197         c
            566-710             AC107426.13        22844-22988         c
            711-827             AC107426.13        22598-22714         c
            828-1399            AC107426.13        18361-18932         c
            1400-1513           AC107426.13        15128-15241         c
            1514-1642           AC107426.13        9144-9272           c
            1643-1708           AC107426.13        7625-7690           c
            1709-3879           AC107426.13        4874-7044           c
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q25.1"
     gene            1..3879
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /note="eukaryotic translation initiation factor 2A"
                     /db_xref="GeneID:83939"
                     /db_xref="HGNC:HGNC:3254"
                     /db_xref="MIM:609234"
     exon            1..44
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..1774
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /note="isoform 1 is encoded by transcript variant 1; 65
                     kDa eukaryotic translation initiation factor 2A;
                     eukaryotic translation initiation factor 2A, 65kDa"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 2A
                     isoform 1"
                     /protein_id="NP_114414.2"
                     /db_xref="CCDS:CCDS46935.1"
                     /db_xref="GeneID:83939"
                     /db_xref="HGNC:HGNC:3254"
                     /db_xref="MIM:609234"
                     /translation="MAPSTPLLTVRGSEGLYMVNGPPHFTESTVFPRESGKNCKVCIF
                     SKDGTLFAWGNGEKVNIISVTNKGLLHSFDLLKAVCLEFSPKNTVLATWQPYTTSKDG
                     TAGIPNLQLYDVKTGTCLKSFIQKKMQNWCPSWSEDETLCARNVNNEVHFFENNNFNT
                     IANKLHLQKINDFVLSPGPQPYKVAVYVPGSKGAPSFVRLYQYPNFAGPHAALANKSF
                     FKADKVTMLWNKKATAVLVIASTDVDKTGASYYGEQTLHYIATNGESAVVQLPKNGPI
                     YDVVWNSSSTEFCAVYGFMPAKATIFNLKCDPVFDFGTGPRNAAYYSPHGHILVLAGF
                     GNLRGQMEVWDVKNYKLISKPVASDSTYFAWCPDGEHILTATCAPRLRVNNGYKIWHY
                     TGSILHKYDVPSNAELWQVSWQPFLDGIFPAKTITYQAVPSEVPNEEPKVATAYRPPA
                     LRNKPITNSKLHEEEPPQNMKPQSGNDKPLSKTALKNQRKHEAKKAAKQEARSDKSPD
                     LAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAIEQLKEQAATGKQLEKNQLE
                     KIQKETALLQELEDLELGI"
     exon            45..114
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /inference="alignment:Splign:2.1.0"
     exon            115..189
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /inference="alignment:Splign:2.1.0"
     exon            190..308
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /inference="alignment:Splign:2.1.0"
     exon            309..408
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /inference="alignment:Splign:2.1.0"
     exon            409..491
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /inference="alignment:Splign:2.1.0"
     exon            492..565
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /inference="alignment:Splign:2.1.0"
     exon            566..710
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /inference="alignment:Splign:2.1.0"
     exon            711..827
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /inference="alignment:Splign:2.1.0"
     exon            828..1399
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1513
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /inference="alignment:Splign:2.1.0"
     exon            1514..1642
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1708
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /inference="alignment:Splign:2.1.0"
     exon            1709..3879
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2084..2089
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /note="hexamer: AACAAA"
     polyA_site      2105
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /note="major polyA site"
     regulatory      3857..3862
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
                     /note="hexamer: AATAAA"
     polyA_site      3879
                     /gene="EIF2A"
                     /gene_synonym="CDA02; EIF-2A; MST089; MSTP004; MSTP089"
ORIGIN      
        1 ctctttccgg gacaacatgg cgccgtccac gccgctcttg acagtccgag gatcagaagg
       61 actgtacatg gtgaatggac caccacattt tacagaaagc acagtgtttc caagggaatc
      121 tgggaagaat tgcaaagtct gtatctttag taaggatggg accttgtttg cctggggcaa
      181 tggagaaaaa gtaaatatta tcagtgtcac taacaaggga ctactgcact ccttcgacct
      241 cctgaaggca gtttgccttg aattctcacc caaaaatact gtcctggcaa cgtggcagcc
      301 ttacactact tctaaagatg gcacagctgg gatacccaac ctacaacttt atgatgtgaa
      361 aactgggaca tgtttgaaat ctttcatcca gaaaaaaatg caaaattggt gtccatcctg
      421 gtcagaagat gaaactcttt gtgcccgcaa tgttaacaat gaagttcact tctttgaaaa
      481 caacaatttt aacacaattg caaataaatt gcatttgcaa aaaattaatg attttgtatt
      541 atcacctgga ccccaaccat acaaggtggc tgtctatgtt ccaggaagta aaggtgcacc
      601 ttcatttgtt agattatatc agtaccccaa ctttgctgga cctcatgcag ctttagctaa
      661 taaaagtttc tttaaggcag ataaagttac aatgctgtgg aataaaaaag ctactgctgt
      721 gttggtaata gctagcacag atgttgacaa gacaggagct tcctactatg gagaacaaac
      781 tctacactac attgcaacaa atggagaaag tgctgtagtg caattaccaa aaaatggccc
      841 catttatgat gtagtttgga attctagttc tactgagttt tgtgctgtat atggttttat
      901 gcctgccaaa gcgacaattt tcaacttgaa atgtgatcct gtatttgact ttggaactgg
      961 tcctcgtaat gcagcctact atagccctca tggacatata ttagtattag ctggatttgg
     1021 aaatctgagg ggacaaatgg aagtgtggga tgtgaaaaac tacaaactta tttctaaacc
     1081 ggtggcttct gattctacat attttgcttg gtgcccggat ggtgagcata ttttaacagc
     1141 tacatgtgct cccaggttac gggttaataa tggatacaaa atttggcatt atactggctc
     1201 tatcttgcac aagtatgatg tgccatcaaa tgcagaatta tggcaggttt cttggcagcc
     1261 atttttggat ggaatatttc cagcaaaaac aataacttac caagcagttc caagtgaagt
     1321 acccaatgag gaacctaaag ttgcaacagc ttatagaccc ccagctttaa gaaataaacc
     1381 aatcaccaat tccaaattgc atgaagagga accacctcag aatatgaaac cacaatcagg
     1441 aaacgataag ccattatcaa aaacagctct taaaaatcaa aggaagcatg aagctaagaa
     1501 agctgcaaag caggaagcaa gaagtgacaa gagtccagat ttggcaccta ctcctgcccc
     1561 acagagcaca ccacgaaaca ctgtctctca gtcaatttct ggggaccctg agatagacaa
     1621 aaaaatcaag aacctaaaga agaaactgaa agcaatcgaa caactgaaag aacaagcagc
     1681 aactggaaaa cagctagaaa aaaatcagtt ggagaaaatt cagaaagaaa cagcccttct
     1741 ccaggagctg gaagatttgg aattgggtat ttaaagattc acggaaagca agttgatgac
     1801 cagaaatcag tgcaaacaca tcttctgtta aacccattgg tatacacaga atattcctgt
     1861 gcccacactt aatgtcaatc tataatttta accatttatc caagattcta ctaagtgtaa
     1921 aattatttaa taatgtctat taaattgata tttatatctt gcatcctata tcatgtcaat
     1981 atgtgatata gaaaagagat acgtgaattt tttagctaag cttgacagat tgaaagacaa
     2041 gtgtcatttt tttttgtaga gggtgatata taccatgtaa atgaacaaag acattttaaa
     2101 tttaatcact gtttttatta taagttcctt agttcagata tcctttatta atttataaga
     2161 tgtgtaaaac tacttaattc tcacaagact ctatgaagta attcttttaa tctctatttt
     2221 ataaatgaaa aaactaaggc cataaattta agaaatttgt cagtgtctca aagctaggaa
     2281 atggccatct aatcccagag cctatgcttt aggtcactat gatatatact actcctgtat
     2341 cctcaagtaa tgtatcattt agtaacacta agcaccctgc ttagttttca tggatactgc
     2401 caaattgccc tatctcaagc tttatacttg caccatttaa agttttccac actgttggca
     2461 atatcagaag tgataaggct ttgaaatgtt cgccactttg ataaagagga aatgattgtt
     2521 tgttactgtt atatacatgt atttttatca gagatgggtt atgtactttt ccacgtttga
     2581 tagttggttg tatttcttta gtgaattgat ttttcttttc ctttgcccat ttttctgttt
     2641 ggatgtttga ctttcttctt aatttgtaag agtatcctat gtagtaacaa aatccttttt
     2701 taaaaatttt taaagaaaaa gtatacacac aggacttttt tttttctaat tcaaaaaata
     2761 caaaggcatg caatgaaaat taagtctgtt ccactaccac attttccagt tttttgtgta
     2821 tctttctaat gatagatacc atatgagtat tcaaatatac atacacatac acttaaaaaa
     2881 aaaagatggc atatactaac tgttctgttt ggggcttttt ggtggtggtg tttgtttgac
     2941 agggtctcac tctgcctccc aggttggagt gcaccttgaa ctcctggact caagtgatcc
     3001 ttcacctcct ccttccaagt atctgacact acaggcttgc atcactatgc ccagcttata
     3061 ctcaccatag tggttctgta ctcccttaaa atattctgga ggccaggcat gctggctcac
     3121 acctgtaatc ccagcacctt gggaggccaa ggtgggtgga tcacctgagg tcaggagttc
     3181 aagaccagcc tgatcaacat ggtgaaaccc catctctact aaaaatacaa aagtatccag
     3241 gcgtggtagc acatacctgt aatcccagct acttgggagg ctgaggtagg aaaattgttt
     3301 gaacccggca gacagaggtt gcagtgagcc gacatcgtgc cattgcactc caagcctggg
     3361 caacaagagt gaaaccgtct cacacacaca caaaaaaaaa tattttggag ctccttctat
     3421 atgagtctct ctctcttatc tctctcagga tgtgtgtatg tgtttgtttc tctggaaaac
     3481 cctgactaat acagattagc tatgtattag gtaatacata atgcctaata cataactaat
     3541 atatggacag gaagttaata gactgactgt gccaccttaa aactcgtatg ttgaagccct
     3601 aacctccaac gtggtggtat tgggagatgg gccttttgga aggtaagtgt gtttgaatga
     3661 ggtgaagagg ttggggactt cgtgatggga ttattgtcct tacaagaaaa gacaccagag
     3721 agcttgttct gtctctttgg gccatgtgaa gacatggtga gaaagcagcc agctgtaagc
     3781 cagagagtcc tcaccagaac ctaaccatgc tggtgatatc ctcatctcag acttctaacc
     3841 tccagaactg tgagaaaata aacttctttg tttaaacca
//
