LOCUS       NM_139265               6401 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens EH domain containing 4 (EHD4), mRNA.
ACCESSION   NM_139265
VERSION     NM_139265.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Malinova,T.S., Angulo-Urarte,A., Nuchel,J., Tauber,M., van der
            Stoel,M.M., Janssen,V., de Haan,A., Groenen,A.G., Tebbens,M.,
            Graupera,M., Plomann,M. and Huveneers,S.
  TITLE     A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin
            trafficking for endothelial migration and angiogenesis
  JOURNAL   Nat Commun 12 (1), 2610 (2021)
   PUBMED   33972531
  REMARK    GeneRIF: A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates
            VE-cadherin trafficking for endothelial migration and angiogenesis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6401)
  AUTHORS   Lin,Y.S., Huang,K.Y., Yu,H.C., Lu,M.C., Fan,C.J., Huang Tseng,H.Y.,
            Jhuang,B.Y., Liu,S.Q., Lai,N.S., Lin,T.H. and Huang,H.B.
  TITLE     Identification of phostensin in association with Eps 15 homology
            domain-containing protein 1 (EHD1) and EHD4
  JOURNAL   Biochem Biophys Res Commun 531 (2), 236-241 (2020)
   PUBMED   32800345
  REMARK    GeneRIF: Identification of phostensin in association with Eps 15
            homology domain-containing protein 1 (EHD1) and EHD4.
REFERENCE   4  (bases 1 to 6401)
  AUTHORS   Jones,T., Naslavsky,N. and Caplan,S.
  TITLE     Eps15 Homology Domain Protein 4 (EHD4) is required for Eps15
            Homology Domain Protein 1 (EHD1)-mediated endosomal recruitment and
            fission
  JOURNAL   PLoS One 15 (9), e0239657 (2020)
   PUBMED   32966336
  REMARK    GeneRIF: Eps15 Homology Domain Protein 4 (EHD4) is required for
            Eps15 Homology Domain Protein 1 (EHD1)-mediated endosomal
            recruitment and fission.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6401)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6401)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   7  (bases 1 to 6401)
  AUTHORS   Sharma,M., Naslavsky,N. and Caplan,S.
  TITLE     A role for EHD4 in the regulation of early endosomal transport
  JOURNAL   Traffic 9 (6), 995-1018 (2008)
   PUBMED   18331452
  REMARK    GeneRIF: EHD4 is involved in the control of trafficking at the
            early endosome and regulates exit of cargo toward both the
            recycling compartment and the late endocytic pathway.
REFERENCE   8  (bases 1 to 6401)
  AUTHORS   George,M., Ying,G., Rainey,M.A., Solomon,A., Parikh,P.T., Gao,Q.,
            Band,V. and Band,H.
  TITLE     Shared as well as distinct roles of EHD proteins revealed by
            biochemical and functional comparisons in mammalian cells and C.
            elegans
  JOURNAL   BMC Cell Biol 8, 3 (2007)
   PUBMED   17233914
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 6401)
  AUTHORS   Kuo,H.J., Tran,N.T., Clary,S.A., Morris,N.P. and Glanville,R.W.
  TITLE     Characterization of EHD4, an EH domain-containing protein expressed
            in the extracellular matrix
  JOURNAL   J Biol Chem 276 (46), 43103-43110 (2001)
   PUBMED   11533061
REFERENCE   10 (bases 1 to 6401)
  AUTHORS   Pohl,U., Smith,J.S., Tachibana,I., Ueki,K., Lee,H.K., Ramaswamy,S.,
            Wu,Q., Mohrenweiser,H.W., Jenkins,R.B. and Louis,D.N.
  TITLE     EHD2, EHD3, and EHD4 encode novel members of a highly conserved
            family of EH domain-containing proteins
  JOURNAL   Genomics 63 (2), 255-262 (2000)
   PUBMED   10673336
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP314075.1, BC051823.1, AK292634.1, CF541306.1, AC039056.7 and
            AC020659.5.
            
            On Nov 23, 2018 this sequence version replaced NM_139265.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.18768.1,
                                           SRR1660807.224957.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2155974
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000220325.9/ ENSP00000220325.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 BP314075.1         1-9
            10-908              BC051823.1         1-899
            909-1778            AK292634.1         906-1775
            1779-2491           BC051823.1         1770-2482
            2492-2623           CF541306.1         293-424             c
            2624-2894           BC051823.1         2615-2885
            2895-3839           AC039056.7         22960-23904         c
            3840-6401           AC020659.5         136815-139376       c
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.1"
     gene            1..6401
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /note="EH domain containing 4"
                     /db_xref="GeneID:30844"
                     /db_xref="HGNC:HGNC:3245"
                     /db_xref="MIM:605892"
     exon            1..299
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..1689
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /note="ortholog of rat pincher; hepatocellular
                     carcinoma-associated protein HCA11; PAST homolog 4;
                     hepatocellular carcinoma-associated protein 10/11"
                     /codon_start=1
                     /product="EH domain-containing protein 4"
                     /protein_id="NP_644670.1"
                     /db_xref="CCDS:CCDS10081.1"
                     /db_xref="GeneID:30844"
                     /db_xref="HGNC:HGNC:3245"
                     /db_xref="MIM:605892"
                     /translation="MFSWMGRQAGGRERAGGADAVQTVTGGLRSLYLRKVLPLEEAYR
                     FHEFHSPALEDADFENKPMILLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIA
                     VMYGETEGSTPGNALVVDPKKPFRKLSRFGNAFLNRFMCSQLPNQVLKSISVIDSPGI
                     LSGEKQRISRGYDFCQVLQWFAERVDRIILLFDAHKLDISDEFSEAIKAFRGQDDKIR
                     VVLNKADQVDTQQLMRVYGALMWSLGKVINTPEVLRVYIGSFWAQPLQNTDNRRLFEA
                     EAQDLFRDIQSLPQKAAVRKLNDLIKRARLAKVHAYIISYLKKEMPSVFGKENKKREL
                     ISRLPEIYIQLQREYQISAGDFPEVKAMQEQLENYDFTKFHSLKPKLIEAVDNMLSNK
                     ISPLMNLISQEETSTPTQLVQGGAFDGTTEGPFNQGYGEGAKEGADEEEWVVAKDKPV
                     YDELFYTLSPINGKISGVNAKKEMVTSKLPNSVLGKIWKLADCDCDGMLDEEEFALAK
                     HLIKIKLDGYELPSSLPPHLVPPSHRKSLPKAD"
     exon            300..476
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            477..574
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            575..987
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1152
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..6401
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2875..2880
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /note="hexamer: TATAAA"
     polyA_site      2894
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /note="major polyA site"
     regulatory      3932..3937
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /note="hexamer: TATAAA"
     polyA_site      3951
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /note="major polyA site"
     regulatory      5299..5304
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /note="hexamer: AATAAA"
     polyA_site      5321
                     /gene="EHD4"
                     /gene_synonym="PAST4"
     regulatory      6378..6383
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /note="hexamer: AATAAA"
     polyA_site      6401
                     /gene="EHD4"
                     /gene_synonym="PAST4"
ORIGIN      
        1 agtgcgagcc ggggagagtc gaacccggag cagggtccca tccgagcgtg gactggcggc
       61 aggatgttca gctggatggg gcggcaggcg ggcgggcgcg aacgcgctgg cggcgcggac
      121 gcggtgcaga cggtgacggg cgggctgcgc tcgctctacc tgcgcaaggt gctgccgctg
      181 gaggaggcgt accgcttcca cgagttccac tcgcctgcgc tggaggacgc cgacttcgag
      241 aacaagccca tgatcctgct ggtgggccag tacagcaccg gcaagaccac cttcatcaga
      301 tacctactgg agcaggattt cccaggcatg aggattggtc cggagcccac cacagactcc
      361 ttcatcgccg tgatgtatgg agagactgag ggcagcaccc cagggaatgc tttagtcgtg
      421 gaccccaaaa agccgtttag aaagctcagt cgctttggaa acgctttcct gaatcgattc
      481 atgtgctcac agctccccaa tcaggtcctg aagagcatca gcgtcatcga cagccccggc
      541 atcctttctg gggagaagca gcgcatcagc cgaggctatg acttctgcca ggtcctgcag
      601 tggtttgccg agagggtgga caggatcatc ctgctctttg acgctcacaa gctggacatc
      661 tcagatgaat tctcagaggc catcaaggcc ttccggggcc aggacgacaa gatccgtgtc
      721 gtgctgaaca aggccgacca agtggacacg cagcagctga tgcgggtcta cggggccctc
      781 atgtggtccc taggcaaggt catcaacacg cccgaggtac tgcgcgtcta cattggctcc
      841 ttctgggcgc agcccctgca gaacacggac aaccgccggc tcttcgaggc tgaggcccag
      901 gacctcttta gagacatcca gagcctcccc cagaaggcag cggtgcgcaa gctcaacgac
      961 ctcatcaagc gagcgaggct ggccaaggtg catgcctaca tcatcagcta cctgaagaag
     1021 gagatgccaa gtgtatttgg aaaggaaaac aagaagagag agcttatcag caggctaccg
     1081 gaaatctaca ttcagctaca gcgagaatac cagatttctg caggggactt ccctgaggtc
     1141 aaggctatgc aggaacagct tgagaactat gacttcacca aattccactc gctgaagccc
     1201 aagctgatcg aggcagtgga caacatgctg agcaacaaga tctcgcccct catgaacctc
     1261 atcagccagg aggagacgag cacgcccacg cagctggtgc agggcggcgc cttcgatggc
     1321 accaccgagg gccccttcaa ccagggctac ggggagggtg ccaaggaggg cgccgacgag
     1381 gaggagtggg tcgtggccaa agacaagccc gtctacgacg agctcttcta cactctgtcg
     1441 cccatcaatg gcaagatatc aggtgtcaac gccaagaagg agatggtgac ctccaagctg
     1501 cccaacagcg tcctgggcaa gatctggaag ctggccgact gcgactgcga cggcatgctt
     1561 gatgaggagg agttcgcgct ggccaagcac ctcatcaaga tcaagctcga cggctacgag
     1621 ctgcccagca gcctgccccc ccacctcgtg cccccctcgc acaggaagtc cctgcccaag
     1681 gccgactgag gggtgggctg cagaacgggg tgggaactgg gggacctggg cctcaggcct
     1741 gctccaccac tgactcaccg aatgaccttg ggcaaggcac tgccctctct gtgccttggt
     1801 ttccccatct gtagaatggg gagggtggac actggaaact agatgacttc tttcacctcc
     1861 aaaattccct tagtttctat gaaaatattg ggggtagggg ggtggattag gagattgaag
     1921 ggttgagaag aaagagaaat tgtccaaaga gtcctcagaa cctgcctgga gaaatgcgca
     1981 tgggggtggg cctggtaagt cccaggaacc acaggaagtg agctaaggct cacccagagc
     2041 agctgggtct ccaggctgcc tgggcttttt gtcctcatga acaactctgg agcagctgtc
     2101 tgtccctcag agggcacctg gaggcagcaa agattatctt atttatttta ttttgttttc
     2161 tgctatattt agagtggcaa aaaaatagag cagagggttt ctgctgtctc cagactgtct
     2221 accaaagaga aggcgacaga tgcctcctgg gtttggaagg gggatgcact gtggatctgc
     2281 catccattct gcagtctcca gcagagcagg cagtcaggcc ccagcgtgcc tccatcccag
     2341 tgccccgatg accatctggt cagccctccc aacctccctt ccagggggct taccagcaaa
     2401 gccatattcg gctaggaatt tggaattcac aacctttatt aacccctggc aaaacttccc
     2461 gtttgcatgt caaatcacat tgaagcctga aaattagctt ttcttactgg tttttccaat
     2521 aagtaatagc aaagcccact tatcatactt aaactacagt aaagaaaaga ggtggttctc
     2581 tgcagtgatc gtttagtgtg gccctcaaac attaaatatc ccgaggtctc cttggtgggt
     2641 ggcaggattt aaattcaatc aaatcctgtc ctagtgtgtg cagtgtcttc ggccctgtgg
     2701 acacaggtga atgagggacc agccctgccc tgggctgttg agaagagatc agtccaccca
     2761 gagttagaca ttgtttttgt agaaaacagg catttattat gtctagggtt ttgtgttttt
     2821 tttttttcct tacaggataa aagcctttat acagaaacaa aatggaacct ttcatataaa
     2881 accttttctt taaaggctgt gtggttcgta tcatgaatgc ccatcacgag gcaggattcg
     2941 ggcacatacc tgtacttctg ctccagagtg tgcgctcccc aaatcccctg agtaaacctc
     3001 tctggactgc tgctcagaca cctgcgtcag gtggaggtgc ctccccctct gtgggcacca
     3061 gcttggggag gtaggtgact tttccttgca aatctccctg cctcatctcc agaaaattgc
     3121 atacctgcat ggcctctctg cattatttat gcaggaagag acacgaaaca gcaatggcca
     3181 catggtatgg gggcctgctg gcaccagaag tgaatgaaat cagaaccgtc ccactgagtc
     3241 ttccaaaagg cacataattt tttttttctg tcacctgcct ttggtgacag ggtcaaaggc
     3301 acagattgaa gagaaaatct tgatttggga gaagcattta tatccagatg cctaccctta
     3361 cactccctag cttactttct acataattta gttttaagag caggaaatgc ataacatggt
     3421 ttttagtctt aacaagcact taaattcatt agacgcatag aaaagaacca tttattattt
     3481 tattattttt atttttattt tgagaggaag tctctgtcac ccaggctgga gtgcagtggc
     3541 gcaatctcag ttcactgcaa cctccgcctc ccaggttcaa gtgattcccc tgcctcagcc
     3601 tcccaagtag ctgggactac aggcacccgc caccacgccc ggctaatttt tgtattttag
     3661 tagagatggg gttttaccat gttggtcaat atggtctcga tctcctgacc tcgtgatcta
     3721 cccacctcgg cctcccaaag tgctgtaacc taagcagaca gttctgtgaa tgcgtgagga
     3781 ccacctgaag tctgcagcca cggtggaggt caccaaaggg tgaactgaga gagaagaggg
     3841 aattcacttg cccaaaggtt tacagatgcc caggcaaact gatttttcca tccagaatta
     3901 tcttgttctt taactctcct caattgtcat atataaacat gaatttatat acatgtgtgt
     3961 cacatgagta tgtcgtttta aaacaacgtg tacatatgta agttttgtct ggatacagaa
     4021 agctgcttta gagggcagca ggttgaggtc tctgatttct ccgtggagga aaacctctca
     4081 ggcactgtga tctccctacc tctgcgctct caggtgggag aggcgtcagg cagcggagtc
     4141 ctttccctgc tggcacctgg taatgttctt ggagagaaag gaaaaaaaaa ggaaatcatt
     4201 tggccacttt tcttaatatc ctcttacatg cagtcattca tcaagaaaag ttgactggaa
     4261 aagaaaacac tagaaaatat gagggatggc gaccaagtga ggacttgtat cttcccagga
     4321 ccagtacaaa gttctgtgaa tgtgtccaca ccaccagctg tctccctacc ccacacggca
     4381 gggcggcagg gccgaccctg gggcctgctc accatcaccg ttgcccttcc tgcaccaccc
     4441 caattttcaa agaacctctt ccacagagtg tttatgtata ggagagaggg tggaagtacc
     4501 taaactctag aactacaaca tctaagaagc tgaggccctg ttttctacac agcatatggt
     4561 atcgcataac acaaaccttc cggtcttgag tttaaaggga aaatgaaaac tccagcctat
     4621 tgctcccaca gtctgcttcc aagttgtcag cctgccagtg agggtctcct gaccccgttt
     4681 gtagagaaac actgcactaa gacttgggaa tctccccagc tcccagggag gggtggcaca
     4741 caacagcctt ccaacctgct tccgcctcct gctccagccc tgcagcgtct gtcaattcat
     4801 ttctcaactc accgcacttg gatggcctgg gggcagggtt gctcttctgg gggagggtca
     4861 ttcattcagt tggcttttgg ctttcctttc tccgggctgg gcctggttct cttttgaaca
     4921 cttgtcgtgc tgcagcaaga gcatctttgg atttggggct gtggagctgt ggttcacccg
     4981 cgggagtcac agccgcggcc tggacctttc cacctcgcac cctcgctcac cctccttcat
     5041 gatgcctgga ggatgcctga gacaattgcc ttgaagatga ggaaagtgtt tcactgttta
     5101 ttttctccat gggatgtagc aggcaagcat gcttaggaca gaaaaccact gagtttactc
     5161 aggtaaatgc gggcctaata caagagtatt gggttccaat gttggttaac caatgcagtt
     5221 aatacgcaat gcttctattt tttttctttt tgtaatctta tttagaagtt tcaaacaaaa
     5281 agacatagtc tgctgatcaa taaacatgga gctgccctct cccactggct cattttccag
     5341 tggctgcgtg ctggcctctc ggagcagagt gtggatccct ggatccaggt tgaaccgggg
     5401 gagaagagtt agggagaggg tgtgcctgtg agccccaact cttcctgtgg agagaaaccc
     5461 aaaatgcagc cgcttcagaa ggaaagcggc cccatcaggt ggggacagaa gagggtaggc
     5521 agcagaggcc aggtctgtgg acaaaagccc cacttcagag tggttggaag ccaggacagg
     5581 agggactcat ggaggtgacc gcttcacagg agtcctttct ccagtcgtgc tagggaggag
     5641 cccctgcctg tccagcactg cccctgcctc ttgctgtacc ttgccagtgt gccctccccc
     5701 cgcccactac catagtaggc atctggcttt tcccaccaac ccatctacct ttttggtggt
     5761 ccagacctac agtctccttg cagagcactc ctcctcccac cctcacttca agtgaggttt
     5821 ggatgagatt gaccacatcc ccttggtcac aaatattggc tcaggaatgg acatgtggcc
     5881 ctgttcaagc ctatgagagc caggaccccc agacttatgt tagaactctt gagagagata
     5941 ctctgtcttc tctgaacctt gcagtatata gaaatgagcc tattgatgca acaatataaa
     6001 tggcactgag aggggtgtcc agagctgctg gggtcagggg agggaggtca gcctggcaga
     6061 agccatcttg tggagcatga aaatgaaaga gaccttgaga aaggccagag gaatccaggc
     6121 ctgtggtgac aacatttgag ctgctggatc aaacctcacc tgaagtcctt cctattccta
     6181 ctagatattt ttttccactg tgtgggccaa tgcattccct ttatactgtg taagccaggc
     6241 atggtggctc acacctataa tcccagcaat gcaggaggct gaagtgggaa gatcgttgag
     6301 gccaggagtt caagaccagc ctgggcaacc taagaagacc ctgtctctaa aaaagaatta
     6361 aatataaata aataaataat aaaaatataa tgtttaaacc a
//
