LOCUS       NP_644670                541 aa            linear   PRI 27-APR-2025
DEFINITION  EH domain-containing protein 4 [Homo sapiens].
ACCESSION   NP_644670
VERSION     NP_644670.1
DBSOURCE    REFSEQ: accession NM_139265.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 541)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (residues 1 to 541)
  AUTHORS   Malinova,T.S., Angulo-Urarte,A., Nuchel,J., Tauber,M., van der
            Stoel,M.M., Janssen,V., de Haan,A., Groenen,A.G., Tebbens,M.,
            Graupera,M., Plomann,M. and Huveneers,S.
  TITLE     A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin
            trafficking for endothelial migration and angiogenesis
  JOURNAL   Nat Commun 12 (1), 2610 (2021)
   PUBMED   33972531
  REMARK    GeneRIF: A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates
            VE-cadherin trafficking for endothelial migration and angiogenesis.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 541)
  AUTHORS   Lin,Y.S., Huang,K.Y., Yu,H.C., Lu,M.C., Fan,C.J., Huang Tseng,H.Y.,
            Jhuang,B.Y., Liu,S.Q., Lai,N.S., Lin,T.H. and Huang,H.B.
  TITLE     Identification of phostensin in association with Eps 15 homology
            domain-containing protein 1 (EHD1) and EHD4
  JOURNAL   Biochem Biophys Res Commun 531 (2), 236-241 (2020)
   PUBMED   32800345
  REMARK    GeneRIF: Identification of phostensin in association with Eps 15
            homology domain-containing protein 1 (EHD1) and EHD4.
REFERENCE   4  (residues 1 to 541)
  AUTHORS   Jones,T., Naslavsky,N. and Caplan,S.
  TITLE     Eps15 Homology Domain Protein 4 (EHD4) is required for Eps15
            Homology Domain Protein 1 (EHD1)-mediated endosomal recruitment and
            fission
  JOURNAL   PLoS One 15 (9), e0239657 (2020)
   PUBMED   32966336
  REMARK    GeneRIF: Eps15 Homology Domain Protein 4 (EHD4) is required for
            Eps15 Homology Domain Protein 1 (EHD1)-mediated endosomal
            recruitment and fission.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 541)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 541)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   7  (residues 1 to 541)
  AUTHORS   Sharma,M., Naslavsky,N. and Caplan,S.
  TITLE     A role for EHD4 in the regulation of early endosomal transport
  JOURNAL   Traffic 9 (6), 995-1018 (2008)
   PUBMED   18331452
  REMARK    GeneRIF: EHD4 is involved in the control of trafficking at the
            early endosome and regulates exit of cargo toward both the
            recycling compartment and the late endocytic pathway.
REFERENCE   8  (residues 1 to 541)
  AUTHORS   George,M., Ying,G., Rainey,M.A., Solomon,A., Parikh,P.T., Gao,Q.,
            Band,V. and Band,H.
  TITLE     Shared as well as distinct roles of EHD proteins revealed by
            biochemical and functional comparisons in mammalian cells and C.
            elegans
  JOURNAL   BMC Cell Biol 8, 3 (2007)
   PUBMED   17233914
  REMARK    Publication Status: Online-Only
REFERENCE   9  (residues 1 to 541)
  AUTHORS   Kuo,H.J., Tran,N.T., Clary,S.A., Morris,N.P. and Glanville,R.W.
  TITLE     Characterization of EHD4, an EH domain-containing protein expressed
            in the extracellular matrix
  JOURNAL   J Biol Chem 276 (46), 43103-43110 (2001)
   PUBMED   11533061
REFERENCE   10 (residues 1 to 541)
  AUTHORS   Pohl,U., Smith,J.S., Tachibana,I., Ueki,K., Lee,H.K., Ramaswamy,S.,
            Wu,Q., Mohrenweiser,H.W., Jenkins,R.B. and Louis,D.N.
  TITLE     EHD2, EHD3, and EHD4 encode novel members of a highly conserved
            family of EH domain-containing proteins
  JOURNAL   Genomics 63 (2), 255-262 (2000)
   PUBMED   10673336
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP314075.1, BC051823.1, AK292634.1, CF541306.1, AC039056.7 and
            AC020659.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.18768.1,
                                           SRR1660807.224957.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2155974
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000220325.9/ ENSP00000220325.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.1"
     Protein         1..541
                     /product="EH domain-containing protein 4"
                     /note="ortholog of rat pincher; hepatocellular
                     carcinoma-associated protein HCA11; PAST homolog 4;
                     hepatocellular carcinoma-associated protein 10/11"
                     /calculated_mol_wt=61044
     Site            1
                     /site_type="acetylation"
                     /note="N-acetylmethionine.
                     /evidence=ECO:0000250|UniProtKB:Q9H4M9; propagated from
                     UniProtKB/Swiss-Prot (Q9H223.1)"
     Region          27..59
                     /region_name="EHD_N"
                     /note="N-terminal EH-domain containing protein; pfam16880"
                     /db_xref="CDD:465295"
     Region          63..303
                     /region_name="EHD"
                     /note="Eps15 homology domain (EHD), C-terminal domain;
                     cd09913"
                     /db_xref="CDD:206740"
     Region          68..75
                     /region_name="G1 motif.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01055"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H223.1)"
     Site            68..75
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206740"
     Site            order(73..76,156,223,225,259..261)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206740"
     Region          94..95
                     /region_name="G2 motif.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01055"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H223.1)"
     Site            97
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206740"
     Site            101..103
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206740"
     Region          156..159
                     /region_name="G3 motif.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01055"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H223.1)"
     Site            156..159
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206740"
     Site            order(158..159,186..187)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206740"
     Site            162
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q9H223.1)"
     Region          222..225
                     /region_name="G4 motif.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01055"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H223.1)"
     Site            222..225
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206740"
     Region          246
                     /region_name="G5 motif.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01055"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H223.1)"
     Site            259..261
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206740"
     Region          292..397
                     /region_name="DUF5600"
                     /note="Domain of unknown function (DUF5600); pfam18150"
                     /db_xref="CDD:465667"
     Region          441..534
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Site            451
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0007744|PubMed:15592455; propagated from
                     UniProtKB/Swiss-Prot (Q9H223.1)"
     Site            459
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163;
                     propagated from UniProtKB/Swiss-Prot (Q9H223.1)"
     CDS             1..541
                     /gene="EHD4"
                     /gene_synonym="PAST4"
                     /coded_by="NM_139265.4:64..1689"
                     /db_xref="CCDS:CCDS10081.1"
                     /db_xref="GeneID:30844"
                     /db_xref="HGNC:HGNC:3245"
                     /db_xref="MIM:605892"
ORIGIN      
        1 mfswmgrqag greraggada vqtvtgglrs lylrkvlple eayrfhefhs paledadfen
       61 kpmillvgqy stgkttfiry lleqdfpgmr igpepttdsf iavmygeteg stpgnalvvd
      121 pkkpfrklsr fgnaflnrfm csqlpnqvlk sisvidspgi lsgekqrisr gydfcqvlqw
      181 faervdriil lfdahkldis defseaikaf rgqddkirvv lnkadqvdtq qlmrvygalm
      241 wslgkvintp evlrvyigsf waqplqntdn rrlfeaeaqd lfrdiqslpq kaavrklndl
      301 ikrarlakvh ayiisylkke mpsvfgkenk krelisrlpe iyiqlqreyq isagdfpevk
      361 amqeqlenyd ftkfhslkpk lieavdnmls nkisplmnli sqeetstptq lvqggafdgt
      421 tegpfnqgyg egakegadee ewvvakdkpv ydelfytlsp ingkisgvna kkemvtsklp
      481 nsvlgkiwkl adcdcdgmld eeefalakhl ikikldgyel psslpphlvp pshrkslpka
      541 d
//
