LOCUS       NM_014601               3506 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens EH domain containing 2 (EHD2), mRNA.
ACCESSION   NM_014601
VERSION     NM_014601.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Li,W., Li,G. and Cao,L.
  TITLE     Circular RNA Eps15-homology domain-containing protein 2 induce
            resistance of renal cell carcinoma to sunitinib via
            microRNA-4731-5p/ABCF2 axis
  JOURNAL   Bioengineered 13 (4), 9729-9740 (2022)
   PUBMED   35412955
  REMARK    GeneRIF: Circular RNA Eps15-homology domain-containing protein 2
            induce resistance of renal cell carcinoma to sunitinib via
            microRNA-4731-5p/ABCF2 axis.
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Zhang,M.S., Cui,J.D., Lee,D., Yuen,V.W., Chiu,D.K., Goh,C.C.,
            Cheu,J.W., Tse,A.P., Bao,M.H., Wong,B.P.Y., Chen,C.Y., Wong,C.M.,
            Ng,I.O. and Wong,C.C.
  TITLE     Hypoxia-induced macropinocytosis represents a metabolic route for
            liver cancer
  JOURNAL   Nat Commun 13 (1), 954 (2022)
   PUBMED   35177645
  REMARK    GeneRIF: Hypoxia-induced macropinocytosis represents a metabolic
            route for liver cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3506)
  AUTHORS   Guan,C., Lu,C., Xiao,M. and Chen,W.
  TITLE     EHD2 Overexpression Suppresses the Proliferation, Migration, and
            Invasion in Human Colon Cancer
  JOURNAL   Cancer Invest 39 (4), 297-309 (2021)
   PUBMED   33356637
  REMARK    GeneRIF: EHD2 Overexpression Suppresses the Proliferation,
            Migration, and Invasion in Human Colon Cancer.
REFERENCE   4  (bases 1 to 3506)
  AUTHORS   Fan,H., Liu,T., Tian,H. and Zhang,S.
  TITLE     TUSC8 inhibits the development of osteosarcoma by sponging
            miR-197-3p and targeting EHD2
  JOURNAL   Int J Mol Med 46 (4), 1311-1320 (2020)
   PUBMED   32945345
  REMARK    GeneRIF: TUSC8 inhibits the development of osteosarcoma by sponging
            miR1973p and targeting EHD2.
            Erratum:[Int J Mol Med. 2021 Apr;47(4):58. doi:
            10.3892/ijmm.2021.4891.. PMID: 33655323]
REFERENCE   5  (bases 1 to 3506)
  AUTHORS   Matthaeus,C., Lahmann,I., Kunz,S., Jonas,W., Melo,A.A., Lehmann,M.,
            Larsson,E., Lundmark,R., Kern,M., Bluher,M., Olschowski,H.,
            Kompa,J., Brugger,B., Muller,D.N., Haucke,V., Schurmann,A.,
            Birchmeier,C. and Daumke,O.
  TITLE     EHD2-mediated restriction of caveolar dynamics regulates cellular
            fatty acid uptake
  JOURNAL   Proc Natl Acad Sci U S A 117 (13), 7471-7481 (2020)
   PUBMED   32170013
  REMARK    GeneRIF: EHD2-mediated restriction of caveolar dynamics regulates
            cellular fatty acid uptake.
REFERENCE   6  (bases 1 to 3506)
  AUTHORS   Aboulaich,N., Vainonen,J.P., Stralfors,P. and Vener,A.V.
  TITLE     Vectorial proteomics reveal targeting, phosphorylation and specific
            fragmentation of polymerase I and transcript release factor (PTRF)
            at the surface of caveolae in human adipocytes
  JOURNAL   Biochem J 383 (Pt 2), 237-248 (2004)
   PUBMED   15242332
REFERENCE   7  (bases 1 to 3506)
  AUTHORS   Park,S.Y., Ha,B.G., Choi,G.H., Ryu,J., Kim,B., Jung,C.Y. and Lee,W.
  TITLE     EHD2 interacts with the insulin-responsive glucose transporter
            (GLUT4) in rat adipocytes and may participate in insulin-induced
            GLUT4 recruitment
  JOURNAL   Biochemistry 43 (23), 7552-7562 (2004)
   PUBMED   15182197
REFERENCE   8  (bases 1 to 3506)
  AUTHORS   Naslavsky,N., Boehm,M., Backlund,P.S. Jr. and Caplan,S.
  TITLE     Rabenosyn-5 and EHD1 interact and sequentially regulate protein
            recycling to the plasma membrane
  JOURNAL   Mol Biol Cell 15 (5), 2410-2422 (2004)
   PUBMED   15020713
REFERENCE   9  (bases 1 to 3506)
  AUTHORS   Guilherme,A., Soriano,N.A., Bose,S., Holik,J., Bose,A.,
            Pomerleau,D.P., Furcinitti,P., Leszyk,J., Corvera,S. and Czech,M.P.
  TITLE     EHD2 and the novel EH domain binding protein EHBP1 couple
            endocytosis to the actin cytoskeleton
  JOURNAL   J Biol Chem 279 (11), 10593-10605 (2004)
   PUBMED   14676205
REFERENCE   10 (bases 1 to 3506)
  AUTHORS   Pohl,U., Smith,J.S., Tachibana,I., Ueki,K., Lee,H.K., Ramaswamy,S.,
            Wu,Q., Mohrenweiser,H.W., Jenkins,R.B. and Louis,D.N.
  TITLE     EHD2, EHD3, and EHD4 encode novel members of a highly conserved
            family of EH domain-containing proteins
  JOURNAL   Genomics 63 (2), 255-262 (2000)
   PUBMED   10673336
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK315548.1, DB260808.1,
            BC014445.1 and BG677716.1.
            
            On Nov 23, 2018 this sequence version replaced NM_014601.3.
            
            Summary: This gene encodes a member of the EH domain-containing
            protein family. These proteins are characterized by a C-terminal
            EF-hand domain, a nucleotide-binding consensus site at the N
            terminus and a bipartite nuclear localization signal. The encoded
            protein interacts with the actin cytoskeleton through an N-terminal
            domain and also binds to an EH domain-binding protein through the
            C-terminal EH domain. This interaction appears to connect
            clathrin-dependent endocytosis to actin, suggesting that this gene
            product participates in the endocytic pathway. [provided by RefSeq,
            Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC014445.1, SRR1660809.52005.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263277.8/ ENSP00000263277.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1804              AK315548.1         3-1806
            1805-1887           DB260808.1         466-548
            1888-2661           BC014445.1         1878-2651
            2662-2896           BG677716.1         92-326
            2897-3506           BC014445.1         2891-3500
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..3506
                     /gene="EHD2"
                     /gene_synonym="PAST2"
                     /note="EH domain containing 2"
                     /db_xref="GeneID:30846"
                     /db_xref="HGNC:HGNC:3243"
                     /db_xref="MIM:605890"
     exon            1..117
                     /gene="EHD2"
                     /gene_synonym="PAST2"
                     /inference="alignment:Splign:2.1.0"
     exon            118..576
                     /gene="EHD2"
                     /gene_synonym="PAST2"
                     /inference="alignment:Splign:2.1.0"
     CDS             173..1804
                     /gene="EHD2"
                     /gene_synonym="PAST2"
                     /note="PAST homolog 2"
                     /codon_start=1
                     /product="EH domain-containing protein 2"
                     /protein_id="NP_055416.2"
                     /db_xref="CCDS:CCDS12704.1"
                     /db_xref="GeneID:30846"
                     /db_xref="HGNC:HGNC:3243"
                     /db_xref="MIM:605890"
                     /translation="MFSWLKRGGARGQQPEAIRTVTSALKELYRTKLLPLEEHYRFGA
                     FHSPALEDADFDGKPMVLVAGQYSTGKTSFIQYLLEQEVPGSRVGPEPTTDCFVAVMH
                     GDTEGTVPGNALVVDPDKPFRKLNPFGNTFLNRFMCAQLPNQVLESISIIDTPGILSG
                     AKQRVSRGYDFPAVLRWFAERVDLIILLFDAHKLEISDEFSEAIGALRGHEDKIRVVL
                     NKADMVETQQLMRVYGALMWALGKVVGTPEVLRVYIGSFWSQPLLVPDNRRLFELEEQ
                     DLFRDIQGLPRHAALRKLNDLVKRARLVRVHAYIISYLKKEMPSVFGKENKKKQLILK
                     LPVIFAKIQLEHHISPGDFPDCQKMQELLMAHDFTKFHSLKPKLLEALDEMLTHDIAK
                     LMPLLRQEELESTEVGVQGGAFEGTHMGPFVERGPDEAMEDGEEGSDDEAEWVVTKDK
                     SKYDEIFYNLAPADGKLSGSKAKTWMVGTKLPNSVLGRIWKLSDVDRDGMLDDEEFAL
                     ASHLIEAKLEGHGLPANLPRRLVPPSKRRHKGSAE"
     exon            577..674
                     /gene="EHD2"
                     /gene_synonym="PAST2"
                     /inference="alignment:Splign:2.1.0"
     exon            675..1087
                     /gene="EHD2"
                     /gene_synonym="PAST2"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1252
                     /gene="EHD2"
                     /gene_synonym="PAST2"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..3506
                     /gene="EHD2"
                     /gene_synonym="PAST2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3484..3489
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EHD2"
                     /gene_synonym="PAST2"
                     /note="hexamer: AATAAA"
     polyA_site      3506
                     /gene="EHD2"
                     /gene_synonym="PAST2"
                     /note="major polyA site"
ORIGIN      
        1 agacggctcc ggacgggacc gcgagcacag gccgctccgc gggcgcttcg gatcctcgcg
       61 ggaccccacc ctctcccagc ctgcccagcc cgctgcagcc gccagcgcgc cccgtcggca
      121 gctctccatc tgcacgtctc tccgtgaacc ccgtgagcgg tgtgcagcca ccatgttcag
      181 ctggctgaag cggggcgggg cacggggcca gcagcccgag gccatccgca cggtgacctc
      241 ggccctcaag gagctgtacc gcacgaagct gctgccgctg gaggagcact accgctttgg
      301 ggccttccac tcgccggccc tggaggacgc agacttcgac ggcaagccca tggtgctggt
      361 ggccggccag tacagcacgg gcaagaccag cttcatccag tacctgctgg agcaggaggt
      421 gcccggctcc cgcgtggggc ctgagcccac caccgactgc tttgtggccg tcatgcacgg
      481 ggacactgag ggcaccgtgc ccggcaacgc cctcgtcgtg gacccggaca agcccttccg
      541 caaactcaac cctttcggaa acaccttcct caacaggttc atgtgtgccc agctccctaa
      601 tcaggtcctg gagagcatca gcatcatcga caccccgggt atcctgtcgg gtgccaagca
      661 gagagtgagc cgcggctacg acttcccggc cgtgctgcgc tggttcgcgg agcgcgtgga
      721 cctcatcatc ctgctctttg atgcgcacaa gctggagatc tcggacgagt tctcagaggc
      781 catcggcgcg ttgcggggcc atgaggacaa gatccgcgtg gtgctcaaca aggccgacat
      841 ggtggagacg cagcagctga tgcgcgtcta cggcgcgctc atgtgggcgc tgggcaaggt
      901 ggtgggcacg cccgaggtgc tgcgcgtcta catcggctcc ttctggtccc agcccctcct
      961 cgtgcccgac aaccggcgcc tcttcgagct ggaggagcag gacctcttcc gcgacatcca
     1021 gggcctgccc cggcacgcag ccttgcgcaa gctcaacgac ctggtgaaga gggcccggct
     1081 ggtgcgagtt cacgcttaca tcatcagcta cctgaagaag gagatgccct ctgtgtttgg
     1141 gaaggagaac aagaagaagc agctgatcct caaactgccc gtcatctttg cgaagattca
     1201 gctggaacat cacatctccc ctggggactt tcctgattgc cagaaaatgc aggagctgct
     1261 gatggcgcac gacttcacca agtttcactc gctgaagccg aagctgctag aggcactgga
     1321 cgagatgctg acgcacgaca tcgccaagct catgcccctg ctgcggcagg aggagctgga
     1381 gagcaccgag gtgggcgtgc aggggggcgc ttttgagggc acccacatgg gcccgtttgt
     1441 ggagcgggga cctgacgagg ccatggagga cggcgaggag ggctcggacg acgaggccga
     1501 gtgggtggtg accaaggaca agtccaaata cgacgagatc ttctacaacc tggcgcctgc
     1561 cgacggcaag ctgagcggct ccaaggccaa gacctggatg gtggggacca agctccccaa
     1621 ctcagtgctg gggcgcatct ggaagctcag cgatgtggac cgcgacggca tgctggatga
     1681 tgaggagttc gcgctggcca gccacctcat cgaggccaag ctggaaggcc acgggctgcc
     1741 cgccaacctg ccccgtcgcc tggtgccacc ctccaagcga cgccacaagg gctccgccga
     1801 gtgagccggc ccccctccca tggccctgct gtggctcccc agctccagtc ggctgcacgc
     1861 acacccctgc tccggctcac acacgccctg cctgccctcc ctgcccagct gtaaggaccg
     1921 ggggtctccc tcctcactac cgccagacac cccggtggaa gcatttagag gggaccacgg
     1981 gagggacaag gcttctctgt ccgcccttca cacctccagc ctcacgttca cttaggcaca
     2041 tcacacacac actggcacac gcaggcatcc atccatccgt cattcattca aatatttatt
     2101 gagcacctac tatgtgccca gccctgttct aggcactggg cattaccata gagaacaaaa
     2161 tagacaaata catctgccct catggaaggt gacgttccca ggagagggca cctacacagt
     2221 cacgcaaaca cacactaatt cctggcaggg cccccagccc ctcccctggc tgagcagccc
     2281 tgtggctgaa atgactagca gataaacaga cccccttctg ctccgcttcc tcctgcccag
     2341 ccaggcaaca ccctcaaccg gctccatcac atcctcaggt ctcgggacca tggggggctc
     2401 agaggggaga cacacctact gcttcctcag atgggcccct ccgcagcccc ttcccttgct
     2461 cggggaaagc ccccaattct gcccacaccc atttatttcc ttccttcctt ccttcttttc
     2521 tttccttcct tccttctttt ttgtttttgc ccccaattct gcccataccc atttctttct
     2581 ttccttcctt ccttcttttt tgtttttgcc cccagttctg tccacacccc ttccctttcc
     2641 tgtcctgtcc tttctttctt ttttgataga atcttgctct gtcgcccagg ctggagtgca
     2701 gtggtgagat ctcagctcac tgcaacctcc acctcctggg ttgaagtgat tctcgtgcct
     2761 cagcctcctg agtagctggg actgcaggca cgcgccacca cgcccagcta atttttgtat
     2821 ttgagtagag acggggtttc accatgttgg ccaggctggt ctcgaactcc gcatctcagg
     2881 tgatctgctc gccttggcct cccaaagtga tgggattaca ggcatgagcc accgtgcccg
     2941 gcttcacacc catttcttta aaaaggatcc cgtagcaggc agaaaagccc cttccatcct
     3001 gctcctctga tactgtgccc ccttggagat atttccgtcc tccacccacg tgtctgtggc
     3061 tggaactgcc cagcctgctc ctggccccct ggaagcctcc ccacagctgg taatctggac
     3121 ttaaggattg ctgggccacc gcctctctgc ctaccaccat tccatattta agtggagccc
     3181 ctacgtagaa aggccccggg gctttatttt agtctccttt tcagggatgt cgtgggcggg
     3241 ggagggggtt cttggtgcta cagccctctc cccaccccta aagggacgcc gacgctgttt
     3301 gctgccttca ccacatatta gtgcttgacc ctggcagggg accccatgga aaagatgggg
     3361 aagagcaaaa tacatggaga cgacgcaccc tccaggatgc tcgctgggat tcccacgccc
     3421 accactgtcc cccaccccat ggctgggagg ggcctctgaa cggaacagtg tccccacaga
     3481 gcgaataaag ccaaggcttc ttccca
//
