LOCUS       NM_001354215            5072 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens EH domain binding protein 1 (EHBP1), transcript
            variant 8, mRNA.
ACCESSION   NM_001354215 XM_017003645
VERSION     NM_001354215.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Rai,A., Bleimling,N., Vetter,I.R. and Goody,R.S.
  TITLE     The mechanism of activation of the actin binding protein EHBP1 by
            Rab8 family members
  JOURNAL   Nat Commun 11 (1), 4187 (2020)
   PUBMED   32826901
  REMARK    GeneRIF: The mechanism of activation of the actin binding protein
            EHBP1 by Rab8 family members.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Beikzadeh,B., Angaji,S.A. and Abolhasani,M.
  TITLE     Association study between common variations in some candidate genes
            and prostate adenocarcinoma predisposition through multi-stage
            approach in Iranian population
  JOURNAL   BMC Med Genet 21 (1), 81 (2020)
   PUBMED   32295536
  REMARK    GeneRIF: The purpose of this study was to evaluate the association
            of rs16901979, rs4242382 and rs1447295 on 8q24 locus, rs2735839
            (KLK3 gene) and rs721048 (EHBP1 gene) with prostate adenocarcinoma
            through multi-stage approach to identify the polymorphisms
            associated with prostate cancer and use them as screening factors
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5072)
  AUTHORS   Li,Z., Schulze,R.J., Weller,S.G., Krueger,E.W., Schott,M.B.,
            Zhang,X., Casey,C.A., Liu,J., Stockli,J., James,D.E. and
            McNiven,M.A.
  TITLE     A novel Rab10-EHBP1-EHD2 complex essential for the autophagic
            engulfment of lipid droplets
  JOURNAL   Sci Adv 2 (12), e1601470 (2016)
   PUBMED   28028537
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5072)
  AUTHORS   Willer,C.J., Schmidt,E.M., Sengupta,S., Peloso,G.M., Gustafsson,S.,
            Kanoni,S., Ganna,A., Chen,J., Buchkovich,M.L., Mora,S.,
            Beckmann,J.S., Bragg-Gresham,J.L., Chang,H.Y., Demirkan,A., Den
            Hertog,H.M., Do,R., Donnelly,L.A., Ehret,G.B., Esko,T.,
            Feitosa,M.F., Ferreira,T., Fischer,K., Fontanillas,P., Fraser,R.M.,
            Freitag,D.F., Gurdasani,D., Heikkila,K., Hypponen,E., Isaacs,A.,
            Jackson,A.U., Johansson,A., Johnson,T., Kaakinen,M., Kettunen,J.,
            Kleber,M.E., Li,X., Luan,J., Lyytikainen,L.P., Magnusson,P.K.E.,
            Mangino,M., Mihailov,E., Montasser,M.E., Muller-Nurasyid,M.,
            Nolte,I.M., O'Connell,J.R., Palmer,C.D., Perola,M., Petersen,A.K.,
            Sanna,S., Saxena,R., Service,S.K., Shah,S., Shungin,D., Sidore,C.,
            Song,C., Strawbridge,R.J., Surakka,I., Tanaka,T., Teslovich,T.M.,
            Thorleifsson,G., Van den Herik,E.G., Voight,B.F., Volcik,K.A.,
            Waite,L.L., Wong,A., Wu,Y., Zhang,W., Absher,D., Asiki,G.,
            Barroso,I., Been,L.F., Bolton,J.L., Bonnycastle,L.L., Brambilla,P.,
            Burnett,M.S., Cesana,G., Dimitriou,M., Doney,A.S.F., Doring,A.,
            Elliott,P., Epstein,S.E., Ingi Eyjolfsson,G., Gigante,B.,
            Goodarzi,M.O., Grallert,H., Gravito,M.L., Groves,C.J., Hallmans,G.,
            Hartikainen,A.L., Hayward,C., Hernandez,D., Hicks,A.A., Holm,H.,
            Hung,Y.J., Illig,T., Jones,M.R., Kaleebu,P., Kastelein,J.J.P.,
            Khaw,K.T., Kim,E., Klopp,N., Komulainen,P., Kumari,M.,
            Langenberg,C., Lehtimaki,T., Lin,S.Y., Lindstrom,J., Loos,R.J.F.,
            Mach,F., McArdle,W.L., Meisinger,C., Mitchell,B.D., Muller,G.,
            Nagaraja,R., Narisu,N., Nieminen,T.V.M., Nsubuga,R.N., Olafsson,I.,
            Ong,K.K., Palotie,A., Papamarkou,T., Pomilla,C., Pouta,A.,
            Rader,D.J., Reilly,M.P., Ridker,P.M., Rivadeneira,F., Rudan,I.,
            Ruokonen,A., Samani,N., Scharnagl,H., Seeley,J., Silander,K.,
            Stancakova,A., Stirrups,K., Swift,A.J., Tiret,L.,
            Uitterlinden,A.G., van Pelt,L.J., Vedantam,S., Wainwright,N.,
            Wijmenga,C., Wild,S.H., Willemsen,G., Wilsgaard,T., Wilson,J.F.,
            Young,E.H., Zhao,J.H., Adair,L.S., Arveiler,D., Assimes,T.L.,
            Bandinelli,S., Bennett,F., Bochud,M., Boehm,B.O., Boomsma,D.I.,
            Borecki,I.B., Bornstein,S.R., Bovet,P., Burnier,M., Campbell,H.,
            Chakravarti,A., Chambers,J.C., Chen,Y.I., Collins,F.S.,
            Cooper,R.S., Danesh,J., Dedoussis,G., de Faire,U., Feranil,A.B.,
            Ferrieres,J., Ferrucci,L., Freimer,N.B., Gieger,C., Groop,L.C.,
            Gudnason,V., Gyllensten,U., Hamsten,A., Harris,T.B., Hingorani,A.,
            Hirschhorn,J.N., Hofman,A., Hovingh,G.K., Hsiung,C.A.,
            Humphries,S.E., Hunt,S.C., Hveem,K., Iribarren,C., Jarvelin,M.R.,
            Jula,A., Kahonen,M., Kaprio,J., Kesaniemi,A., Kivimaki,M.,
            Kooner,J.S., Koudstaal,P.J., Krauss,R.M., Kuh,D., Kuusisto,J.,
            Kyvik,K.O., Laakso,M., Lakka,T.A., Lind,L., Lindgren,C.M.,
            Martin,N.G., Marz,W., McCarthy,M.I., McKenzie,C.A., Meneton,P.,
            Metspalu,A., Moilanen,L., Morris,A.D., Munroe,P.B., Njolstad,I.,
            Pedersen,N.L., Power,C., Pramstaller,P.P., Price,J.F., Psaty,B.M.,
            Quertermous,T., Rauramaa,R., Saleheen,D., Salomaa,V.,
            Sanghera,D.K., Saramies,J., Schwarz,P.E.H., Sheu,W.H.,
            Shuldiner,A.R., Siegbahn,A., Spector,T.D., Stefansson,K.,
            Strachan,D.P., Tayo,B.O., Tremoli,E., Tuomilehto,J., Uusitupa,M.,
            van Duijn,C.M., Vollenweider,P., Wallentin,L., Wareham,N.J.,
            Whitfield,J.B., Wolffenbuttel,B.H.R., Ordovas,J.M., Boerwinkle,E.,
            Palmer,C.N.A., Thorsteinsdottir,U., Chasman,D.I., Rotter,J.I.,
            Franks,P.W., Ripatti,S., Cupples,L.A., Sandhu,M.S., Rich,S.S.,
            Boehnke,M., Deloukas,P., Kathiresan,S., Mohlke,K.L., Ingelsson,E.
            and Abecasis,G.R.
  CONSRTM   Global Lipids Genetics Consortium
  TITLE     Discovery and refinement of loci associated with lipid levels
  JOURNAL   Nat Genet 45 (11), 1274-1283 (2013)
   PUBMED   24097068
REFERENCE   5  (bases 1 to 5072)
  AUTHORS   Xu,J., Kibel,A.S., Hu,J.J., Turner,A.R., Pruett,K., Zheng,S.L.,
            Sun,J., Isaacs,S.D., Wiley,K.E., Kim,S.T., Hsu,F.C., Wu,W.,
            Torti,F.M., Walsh,P.C., Chang,B.L. and Isaacs,W.B.
  TITLE     Prostate cancer risk associated loci in African Americans
  JOURNAL   Cancer Epidemiol Biomarkers Prev 18 (7), 2145-2149 (2009)
   PUBMED   19549807
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 5072)
  AUTHORS   Wiklund,F.E., Adami,H.O., Zheng,S.L., Stattin,P., Isaacs,W.B.,
            Gronberg,H. and Xu,J.
  TITLE     Established prostate cancer susceptibility variants are not
            associated with disease outcome
  JOURNAL   Cancer Epidemiol Biomarkers Prev 18 (5), 1659-1662 (2009)
   PUBMED   19423541
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 5072)
  AUTHORS   Waters,K.M., Le Marchand,L., Kolonel,L.N., Monroe,K.R., Stram,D.O.,
            Henderson,B.E. and Haiman,C.A.
  TITLE     Generalizability of associations from prostate cancer genome-wide
            association studies in multiple populations
  JOURNAL   Cancer Epidemiol Biomarkers Prev 18 (4), 1285-1289 (2009)
   PUBMED   19318432
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 5072)
  AUTHORS   Gudmundsson,J., Sulem,P., Rafnar,T., Bergthorsson,J.T.,
            Manolescu,A., Gudbjartsson,D., Agnarsson,B.A., Sigurdsson,A.,
            Benediktsdottir,K.R., Blondal,T., Jakobsdottir,M., Stacey,S.N.,
            Kostic,J., Kristinsson,K.T., Birgisdottir,B., Ghosh,S.,
            Magnusdottir,D.N., Thorlacius,S., Thorleifsson,G., Zheng,S.L.,
            Sun,J., Chang,B.L., Elmore,J.B., Breyer,J.P., McReynolds,K.M.,
            Bradley,K.M., Yaspan,B.L., Wiklund,F., Stattin,P., Lindstrom,S.,
            Adami,H.O., McDonnell,S.K., Schaid,D.J., Cunningham,J.M., Wang,L.,
            Cerhan,J.R., St Sauver,J.L., Isaacs,S.D., Wiley,K.E., Partin,A.W.,
            Walsh,P.C., Polo,S., Ruiz-Echarri,M., Navarrete,S., Fuertes,F.,
            Saez,B., Godino,J., Weijerman,P.C., Swinkels,D.W., Aben,K.K.,
            Witjes,J.A., Suarez,B.K., Helfand,B.T., Frigge,M.L.,
            Kristjansson,K., Ober,C., Jonsson,E., Einarsson,G.V., Xu,J.,
            Gronberg,H., Smith,J.R., Thibodeau,S.N., Isaacs,W.B.,
            Catalona,W.J., Mayordomo,J.I., Kiemeney,L.A., Barkardottir,R.B.,
            Gulcher,J.R., Thorsteinsdottir,U., Kong,A. and Stefansson,K.
  TITLE     Common sequence variants on 2p15 and Xp11.22 confer susceptibility
            to prostate cancer
  JOURNAL   Nat Genet 40 (3), 281-283 (2008)
   PUBMED   18264098
REFERENCE   9  (bases 1 to 5072)
  AUTHORS   Guilherme,A., Soriano,N.A., Furcinitti,P.S. and Czech,M.P.
  TITLE     Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated
            GLUT4 recycling in 3T3-L1 adipocytes
  JOURNAL   J Biol Chem 279 (38), 40062-40075 (2004)
   PUBMED   15247266
REFERENCE   10 (bases 1 to 5072)
  AUTHORS   Guilherme,A., Soriano,N.A., Bose,S., Holik,J., Bose,A.,
            Pomerleau,D.P., Furcinitti,P., Leszyk,J., Corvera,S. and Czech,M.P.
  TITLE     EHD2 and the novel EH domain binding protein EHBP1 couple
            endocytosis to the actin cytoskeleton
  JOURNAL   J Biol Chem 279 (11), 10593-10605 (2004)
   PUBMED   14676205
  REMARK    GeneRIF: EHBP1 is an EH domain binding protein that, along with
            EHD2, has a role in coupling endocytosis to the actin cytoskeleton
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB072549.1, AY331186.2,
            DA403235.1, BP361773.1, AY531390.1, BQ215191.1 and BC067215.1.
            
            On Aug 29, 2017 this sequence version replaced XM_017003645.1.
            
            Summary: This gene encodes an Eps15 homology domain binding
            protein. The encoded protein may play a role in endocytic
            trafficking. A single nucleotide polymorphism in this gene is
            associated with an aggressive form of prostate cancer. Alternate
            splicing results in multiple transcript variants. [provided by
            RefSeq, Feb 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.209121.1,
                                           SRR1803616.133889.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                DB072549.1         1-85
            86-714              AY331186.2         56-684
            715-1063            DA403235.1         197-545
            1064-1323           BP361773.1         323-582
            1324-2899           AY531390.1         839-2414
            2900-3063           BQ215191.1         1-164
            3064-4073           AY531390.1         2582-3591
            4074-5072           BC067215.1         3820-4818
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p15"
     gene            1..5072
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /note="EH domain binding protein 1"
                     /db_xref="GeneID:23301"
                     /db_xref="HGNC:HGNC:29144"
                     /db_xref="MIM:609922"
     exon            1..85
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            86..484
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     CDS             381..4073
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /note="isoform 4 is encoded by transcript variant 8; NPF
                     calponin-like protein; testis tissue sperm-binding protein
                     Li 50e"
                     /codon_start=1
                     /product="EH domain-binding protein 1 isoform 4"
                     /protein_id="NP_001341144.1"
                     /db_xref="GeneID:23301"
                     /db_xref="HGNC:HGNC:29144"
                     /db_xref="MIM:609922"
                     /translation="MASVWKRLQRVGKHASKFQFVASYQELMVECTKKWQPDKLVVVW
                     TRRSRRKSSKAHSWQPGIKNPYRGVVVWPVPENIEITVTLFKDPHAEEFEDKEWTFVI
                     ENESPSGRRKALATSSINMKQYASPMPTQTDVKLKFKPLSKKVVSAALQFSLSCIFLR
                     EGKATDEDMQSLASLMSMKQADIGNLDDFEEDNEDDDENRVNQEEKAAKITEIVNQLN
                     ALSSLDEDQDDCIKQANMRSAKSASSSEELINKLNFLDEAEKDLATVNSNPFDDPDAA
                     ELNPFGDPDSEEPITETASPRKTEDSFYNNSYNPFKEVQTPQYLNPFDEPEAFVTIKD
                     SPPQSTKRKNIRPVDMSKYLYADSSKTEEEELDESNPFYEPKSTPPPNNLVNPVQELE
                     TERRVKRKAPAPPVLSPKTGVLNENTVSAGKDLSTSPKPSPIPSPVLGRKPNASQSLL
                     VWCKEVTKNYRGVKITNFTTSWRNGLSFCAILHHFRPDLIDYKSLNPQDIKENNKKAY
                     DGFASIGISRLLEPSDMVLLAIPDKLTVMTYLYQIRAHFSGQELNVVQIEENSSKSTY
                     KVGNYETDTNSSVDQEKFYAELSDLKREPELQQPISGAVDFLSQDDSVFVNDSGVGES
                     ESEHQTPDDHLSPSTASPYCRRTKSDTEPQKSQQSSGRTSGSDDPGICSNTDSTQAQV
                     LLGKKRLLKAETLELSDLYVSDKKKDMSPPFICEETDEQKLQTLDIGSNLEKEKLENS
                     RSLECRSDPESPIKKTSLSPTSKLGYSYSRDLDLAKKKHASLRQTESDPDADRTTLNH
                     ADHSSKIVQHRLLSRQEELKERARVLLEQARRDAALKAGNKHNTNTATPFCNRQLSDQ
                     DEERRRQLRERARQLIAEARSGVKMSELPSYGEMAAEKLKERSKASGDENDNIEIDTN
                     EEIPEGFVVGGGDELTNLENDLDTPEQNSKLVDLKLKKLLEVQPQVANSPSSAAQKAV
                     TESSEQDMKSGTEDLRTERLQKTTERFRNPVVFSKDSTVRKTQLQSFSQYIENRPEMK
                     RQRSIQEDTKKGNEEKAAITETQRKPSEDEVLNKGFKDTSQYVVGELAALENEQKQID
                     TRAALVEKRLRYLMDTGRNTEEEEAMMQEWFMLVNKKNALIRRMNQLSLLEKEHDLER
                     RYELLNRELRAMLAIEDWQKTEAQKRREQLLLDELVALVNKRDALVRDLDAQEKQAEE
                     EDEHLERTLEQNKGKMAKKEEKCVLQ"
     exon            485..542
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            543..638
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            639..692
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            693..874
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1014
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1119
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1242
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1483
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1670
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1849
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1850..1898
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..2801
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2802..2945
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2946..3090
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3091..3198
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3199..3323
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3324..3462
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3463..3569
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3570..3693
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3694..3793
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3794..3867
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3868..3982
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3983..5060
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5032..5037
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /note="hexamer: AATAAA"
     polyA_site      5060
                     /gene="EHBP1"
                     /gene_synonym="HPC12; NACSIN"
                     /note="major polyA site"
ORIGIN      
        1 acgcgcgggg cctggccaat ggcggcgggg cggtggaggt tgagctgccg tgggtagggt
       61 tagtgtgaga ggtgcggcgg gggagccctc cagaataccc atcatatagc ccctgaggtg
      121 gcatggtgat gtctccatga gggaacccct tcccactcat cctgtcacgt atatcatagt
      181 gttcttgact gggccattca tctaagatgg gatttaccct gtgaaacagg gagaagactt
      241 atggacccca agcatcattt cgagttgtag ttgagttttt aaaagacata catgcaaagt
      301 tcctttgctt tggaccctct gcattattaa agctgctgta ttgctaaccc agaactgctc
      361 cagtgtcttg actgatcatc atggcttcag tttggaagag actgcagcgt gtgggaaaac
      421 atgcatccaa gttccagttt gtggcctcct accaggagct catggttgag tgtacgaaga
      481 aatggcaacc agataaactg gtggtagttt ggaccagaag aagccgaagg aagtcttcta
      541 aggcacatag ctggcaacct ggaataaaaa atccctatcg tggtgttgtt gtgtggcctg
      601 ttcctgaaaa cattgaaatc actgtaacac tttttaagga tcctcatgcg gaagaatttg
      661 aagacaaaga gtggacattt gtcatagaaa atgaatcccc ttctggtcga aggaaagctc
      721 ttgctactag cagcatcaat atgaaacagt atgcaagccc tatgccaact cagactgatg
      781 tcaagttaaa attcaagcca ttatctaaaa aagttgtatc tgccgctctt cagttttcat
      841 tatcttgcat ttttctgagg gaaggaaaag ccacagatga agacatgcaa agtttggcta
      901 gtttgatgag tatgaagcag gctgacattg gcaatttaga tgacttcgaa gaagataatg
      961 aagatgatga tgagaacaga gtgaaccaag aagaaaaggc agctaaaatt acagaaattg
     1021 taaaccagtt gaatgctctg agcagcttag atgaagatca agatgactgc ataaagcaag
     1081 caaatatgcg ttcagctaaa tcagccagtt cctctgaaga gcttatcaac aaacttaact
     1141 ttttggatga agcagaaaag gacttggcca ccgtgaattc aaatccattt gatgatcctg
     1201 atgctgcaga attaaatcca tttggagatc ctgactcaga agaacctatc actgaaacag
     1261 cttcacctag aaaaacagaa gactcttttt ataataacag ctataatccc tttaaagagg
     1321 tgcagactcc acagtatttg aacccattcg atgagccaga agcatttgtg accataaagg
     1381 attctcctcc ccagtctaca aaaagaaaaa atataagacc tgtggatatg agcaagtacc
     1441 tctatgctga tagttctaaa actgaagaag aagaattgga tgaatcaaat cctttttatg
     1501 aacctaaatc aactcctcct ccaaataatt tggtaaatcc tgttcaagaa ctagaaactg
     1561 aaaggcgagt gaaaagaaag gccccggctc caccagtcct ctcaccaaaa acaggagtat
     1621 taaatgaaaa cacagtttct gcaggaaaag atctctctac ttctcctaag ccaagcccta
     1681 taccaagtcc tgttttgggg cgaaagccaa atgctagtca gtctttgctt gtatggtgta
     1741 aagaagttac aaagaactac cgaggagtaa aaatcaccaa ttttactaca tcgtggagaa
     1801 atggtttatc tttttgtgca atattacacc actttagacc agatttaatt gactacaagt
     1861 ctctgaatcc tcaagatatt aaagagaaca acaaaaaggc atacgatgga tttgccagca
     1921 taggaatttc ccgattattg gaaccttctg atatggtatt attagcaatt cctgataaac
     1981 tgactgttat gacttatctc tatcaaataa gggcacattt cagtggccaa gaactaaatg
     2041 tcgttcagat agaggaaaac agcagtaaaa gcacatataa agttggaaac tatgaaacag
     2101 atacaaacag ttctgttgat caagaaaaat tctatgcaga gcttagtgat ctgaagcggg
     2161 agcctgaact acaacagcct atcagcggag cagtagactt cttatcacag gatgactctg
     2221 tatttgtaaa tgatagcggg gttggagagt cagaaagtga gcatcaaact cctgatgatc
     2281 accttagtcc aagcacagcc tccccttact gtcgcaggac taaaagtgac acagaacccc
     2341 agaagtctca gcagagctct ggaaggactt caggatctga tgaccctgga atatgttcca
     2401 atacagattc aacccaagca caggttttgt taggcaaaaa gagactattg aaagctgaga
     2461 ctttagaatt gagtgactta tatgttagtg ataagaagaa ggatatgtct ccacccttta
     2521 tttgtgagga gacagatgaa caaaagcttc aaactctaga catcggtagt aacttggaga
     2581 aagaaaaatt agagaattcc agatccttag aatgcagatc agatccagaa tctcctatca
     2641 aaaaaacaag tttatctcct acttctaaac ttggatactc atatagtaga gatctagacc
     2701 ttgctaagaa aaaacatgct tccctgaggc agacggagtc tgatccagat gctgatagaa
     2761 ccactttaaa tcatgcagat cattcatcaa aaatagtcca gcatcgattg ttatctagac
     2821 aagaagaact taaggaaaga gcaagagttc tgcttgagca agcaagaaga gatgcagcct
     2881 taaaggcggg gaataagcac aataccaaca cagccacccc attctgcaac aggcagctaa
     2941 gtgatcaaga tgaagagcga cgtcggcagc tgagagagag agctcgtcag ctaatagcag
     3001 aagctcgatc tggagtgaag atgtcagaac ttcccagcta tggtgaaatg gctgcagaaa
     3061 agttgaaaga aaggtcaaag gcatctggag atgaaaatga taatattgag atagatacta
     3121 acgaggagat ccctgaaggc tttgttgtag gaggtggaga tgaacttact aacttagaaa
     3181 atgaccttga tactcccgaa caaaacagta agttggtgga cttgaagctg aagaagctcc
     3241 tagaagttca gccacaggtg gcaaattcac cctccagtgc tgcccagaaa gctgtaactg
     3301 agagctcaga gcaggacatg aaaagtggca cagaagatct ccggactgaa cgattacaaa
     3361 aaacaacaga acgttttaga aatcctgttg tgttcagcaa agattctaca gtcagaaaaa
     3421 ctcaacttca gtctttcagc caatatattg agaatagacc agagatgaaa aggcagagat
     3481 caatacagga agatacaaag aaaggaaatg aggagaaggc agcgataact gaaactcaga
     3541 ggaagccatc agaagatgaa gtgcttaata aagggttcaa agacaccagt cagtatgtag
     3601 taggagaatt ggcagcacta gagaatgagc aaaagcaaat tgacacccgt gccgcgctgg
     3661 tggagaagcg ccttcgctat ctcatggaca caggaaggaa cacagaagaa gaagaagcta
     3721 tgatgcagga atggtttatg ttagttaata agaaaaatgc cttaataagg agaatgaatc
     3781 agctctctct tctggaaaaa gaacatgatt tagaacgacg gtatgagctg ctgaaccggg
     3841 aattgagggc aatgctagcc attgaagact ggcagaagac cgaggcccag aagcgacgcg
     3901 aacagcttct gctagatgag ctggtggccc tggtgaacaa gcgcgatgcg ctcgtcaggg
     3961 acctggacgc gcaggagaag caggccgaag aagaagatga gcatttggag cgaactctgg
     4021 agcaaaacaa aggcaagatg gccaagaaag aggagaaatg tgttcttcag tagccatcag
     4081 atcagaaaga atctctccca acattttaga gtcttgcttc ccaaaccaga aaaagtcaga
     4141 ctcattgttg atttaaaact tttaacattt tgtttggctg gattgtacta ctttacctct
     4201 actttaccac caccaccctt ttcctccctc ctttccaaat aatatacaga actccaaaat
     4261 agcttcattt aaggattttt ttgtgagtta acaatttcct tgaaatcctg tgaaatagat
     4321 ttgcacagac accttgtgag tgattggtat tggaggtgtt caagaaactg ttcgaaaaag
     4381 aacaaaaaca cttccctcgt tattttctct cattttttga tgagaggaaa atttgaaaca
     4441 ttattcttgt tgttgttggt aatagcataa tgacagtggg aggggtacaa ggggataaga
     4501 aaaatgtcat gatttttttc cggtcctgcc acatgtaaca cttactctgt tacctaaatt
     4561 ttatagttag atcatatcca atctacttat taaactgtgt tctatttacc agtggagttt
     4621 ttctgcagtg gttgcgtttc actgtaagga taatggagtt cctctcctct gctttcctca
     4681 gaggatggtc ctttaacata gccagaaaca agccctgtgg tttgaaggtg agctgtgagg
     4741 atgggactaa ttgatatgca ccagtttaca aagacagtct tatcatccga gaatacacca
     4801 tctttttctc tggataatta tttcttacat catgcttgat tcctacattt tgttgggtct
     4861 caacattggc tcacgaatgc tgttaatatt tattctgtat tgataaaaag tctgtcttgc
     4921 cactacaagt aaatccccca tttaatattt tcttctttag catagcactg tcattttttg
     4981 tgaaaatggt tatgtttatt tattacaata ctgagtcata tataaatttt caataaaagc
     5041 agaaactttc ttaccttaaa aaaaaaaaaa aa
//
