LOCUS       NM_001965               2220 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens early growth response 4 (EGR4), mRNA.
ACCESSION   NM_001965
VERSION     NM_001965.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   He,S., Lin,J., Xu,Y., Lin,L. and Feng,J.
  TITLE     A positive feedback loop between ZNF205-AS1 and EGR4 promotes
            non-small cell lung cancer growth
  JOURNAL   J Cell Mol Med 23 (2), 1495-1508 (2019)
   PUBMED   30556283
  REMARK    GeneRIF: our study demonstrated that the positive feedback loop
            between ZNF205-AS1 and EGR4 promotes non-small cell lung cancer
            (NSCLC) growth, and implied that targeting this feedback loop may
            be promising therapeutic strategy for NSCLC
REFERENCE   3  (bases 1 to 2220)
  AUTHORS   Yin,Y., Morgunova,E., Jolma,A., Kaasinen,E., Sahu,B.,
            Khund-Sayeed,S., Das,P.K., Kivioja,T., Dave,K., Zhong,F.,
            Nitta,K.R., Taipale,M., Popov,A., Ginno,P.A., Domcke,S., Yan,J.,
            Schubeler,D., Vinson,C. and Taipale,J.
  TITLE     Impact of cytosine methylation on DNA binding specificities of
            human transcription factors
  JOURNAL   Science 356 (6337) (2017)
   PUBMED   28473536
REFERENCE   4  (bases 1 to 2220)
  AUTHORS   Sung,S.R., Song,S.H., Kang,K.M., Park,J.E., Nam,Y.J., Shin,Y.J.,
            Cha,D.H., Seo,J.T., Yoon,T.K. and Shim,S.H.
  TITLE     Sequence variations of the EGR4 gene in Korean men with
            spermatogenesis impairment
  JOURNAL   BMC Med Genet 18 (1), 47 (2017)
   PUBMED   28464846
  REMARK    GeneRIF: this study is the first to screen the EGR4 gene in
            relation to male infertility. However, our findings did not clearly
            explain how nonsynonymous EGR4 variations affect spermatogenesis
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2220)
  AUTHORS   Matsuo,T., Dat le,T., Komatsu,M., Yoshimaru,T., Daizumoto,K.,
            Sone,S., Nishioka,Y. and Katagiri,T.
  TITLE     Early growth response 4 is involved in cell proliferation of small
            cell lung cancer through transcriptional activation of its
            downstream genes
  JOURNAL   PLoS One 9 (11), e113606 (2014)
   PUBMED   25411851
  REMARK    GeneRIF: EGR4 directly transactivates specific variants (V3 and V4)
            of the PTHrP gene resulting in subsequent transactivation of the
            RANKL, IL-6 and IL-8 genes via paracrine action of the PTHrP
            protein.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2220)
  AUTHORS   Decker,E.L., Nehmann,N., Kampen,E., Eibel,H., Zipfel,P.F. and
            Skerka,C.
  TITLE     Early growth response proteins (EGR) and nuclear factors of
            activated T cells (NFAT) form heterodimers and regulate
            proinflammatory cytokine gene expression
  JOURNAL   Nucleic Acids Res 31 (3), 911-921 (2003)
   PUBMED   12560487
REFERENCE   7  (bases 1 to 2220)
  AUTHORS   Holst,C., Skerka,C., Lichter,P., Bialonski,A. and Zipfel,P.F.
  TITLE     Genomic organization, chromosomal localization and promoter
            function of the human zinc-finger gene pAT133
  JOURNAL   Hum Mol Genet 2 (4), 367-372 (1993)
   PUBMED   8504297
REFERENCE   8  (bases 1 to 2220)
  AUTHORS   Crosby,S.D., Veile,R.A., Donis-Keller,H., Baraban,J.M., Bhat,R.V.,
            Simburger,K.S. and Milbrandt,J.
  TITLE     Neural-specific expression, genomic structure, and chromosomal
            localization of the gene encoding the zinc-finger transcription
            factor NGFI-C
  JOURNAL   Proc Natl Acad Sci U S A 89 (10), 4739-4743 (1992)
   PUBMED   1584812
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6663. doi:
            10.1073/pnas.89.14.6663.. PMID: 1631170]
REFERENCE   9  (bases 1 to 2220)
  AUTHORS   Muller,H.J., Skerka,C., Bialonski,A. and Zipfel,P.F.
  TITLE     Clone pAT 133 identifies a gene that encodes another human member
            of a class of growth factor-induced genes with almost identical
            zinc-finger domains
  JOURNAL   Proc Natl Acad Sci U S A 88 (22), 10079-10083 (1991)
   PUBMED   1658795
REFERENCE   10 (bases 1 to 2220)
  AUTHORS   Crosby,S.D., Puetz,J.J., Simburger,K.S., Fahrner,T.J. and
            Milbrandt,J.
  TITLE     The early response gene NGFI-C encodes a zinc finger
            transcriptional activator and is a member of the GCGGGGGCG (GSG)
            element-binding protein family
  JOURNAL   Mol Cell Biol 11 (8), 3835-3841 (1991)
   PUBMED   2072895
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC010913.9.
            
            On Oct 16, 2018 this sequence version replaced NM_001965.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.485855.1,
                                           SRR1803615.131529.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000436467.4/ ENSP00000419687.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-367               AC010913.9         205454-205820       c
            368-2220            AC010913.9         203201-205053       c
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p13.2"
     gene            1..2220
                     /gene="EGR4"
                     /gene_synonym="AT133; NGFI-C; NGFIC; PAT133"
                     /note="early growth response 4"
                     /db_xref="GeneID:1961"
                     /db_xref="HGNC:HGNC:3241"
                     /db_xref="MIM:128992"
     exon            1..367
                     /gene="EGR4"
                     /gene_synonym="AT133; NGFI-C; NGFIC; PAT133"
                     /inference="alignment:Splign:2.1.0"
     CDS             232..1692
                     /gene="EGR4"
                     /gene_synonym="AT133; NGFI-C; NGFIC; PAT133"
                     /codon_start=1
                     /product="early growth response protein 4"
                     /protein_id="NP_001956.4"
                     /db_xref="CCDS:CCDS1925.3"
                     /db_xref="GeneID:1961"
                     /db_xref="HGNC:HGNC:3241"
                     /db_xref="MIM:128992"
                     /translation="MLHLSEFSEPDALLVKSTEGCCAEPSAELPRLPARDAPAATGYP
                     GAGDFLSWALNSCGASGDLADSCFLEGPAPTPPPGLSYSGSFFIQAVPEHPHDPEALF
                     NLMSGILGLAPFPGPEAAASRSPLDAPFPAGSDALLPGPPDLYSPDLGAAPFPEAFWE
                     ASPCAGAPSQCLYEPQLSPPDVKPGLRAPPASPALDAVSAFKGPYAPWELLSVGAPGN
                     CGSQGDYQAAPEARFPVIGTKIEDLLSISCPAELPAVPANRLYPSGAYDAFPLAPGDL
                     GEGAEGLPGLLTPPSGEGGSSGDGGEFLASTQPQLSPLGLRSAAAADFPKPLVADIPG
                     SSGVAAPPVPPPPPTPFPQAKARRKGRRGGKCSTRCFCPRPHAKAFACPVESCVRSFA
                     RSDELNRHLRIHTGHKPFQCRICLRNFSRSDHLTTHVRTHTGEKPFACDVCGRRFARS
                     DEKKRHSKVHLKQKARAEERLKGLGFYSLGLSFASL"
     exon            368..2220
                     /gene="EGR4"
                     /gene_synonym="AT133; NGFI-C; NGFIC; PAT133"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2195..2200
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EGR4"
                     /gene_synonym="AT133; NGFI-C; NGFIC; PAT133"
                     /note="hexamer: ATTAAA"
     polyA_site      2220
                     /gene="EGR4"
                     /gene_synonym="AT133; NGFI-C; NGFIC; PAT133"
                     /note="major polyA site"
ORIGIN      
        1 gagcccggca gcgctttgga gaggcgagga gccgccgccc gaggccggtg cgggcgagcg
       61 agggcgccgc ggctccccga ctcctttccc agaggtgagt gcccgaagcc aggagcccgg
      121 gcgcctaggt ctgtgcgctg cggggaaccc ctaccgccag cctccccgcc acccgcgcgc
      181 ccccaagccc agcgggcgag gccccgggcg ccccacagcc ggcgccgcgc catgctccac
      241 cttagcgagt tttccgaacc cgacgcgctc ctcgtcaagt ccactgaagg ctgttgcgcc
      301 gaacccagcg ctgaattgcc ccggctgcct gccagggacg ctcccgcggc caccggctac
      361 cctggagcag gcgacttctt gagctgggct ttgaacagct gcggcgcaag tggggactta
      421 gccgactcct gcttcctgga ggggcctgcg cccacacccc ctcccggcct cagctacagc
      481 ggtagcttct tcattcaggc agtgcccgaa cacccgcacg acccggaggc actcttcaac
      541 ctcatgtcgg gcatcttagg cctggcaccc ttccccggtc cagaggcagc agcgtccaga
      601 tccccgctgg atgccccttt tcctgcgggg tccgatgcct tgctgccggg tccgccggac
      661 ctttactccc cggatctggg cgctgcccct ttcccagagg cgttctggga ggcctcgcct
      721 tgcgcgggtg ccccctcgca gtgcctgtat gagcctcagc tctccccgcc cgacgtcaag
      781 cccggcctcc gggcgcctcc cgcctcgcca gcgctggacg ctgtctctgc cttcaagggt
      841 ccctacgcgc cctgggagct gctttctgtg ggggccccag ggaactgtgg gtcacaggga
      901 gactaccagg ccgccccgga ggctcgtttt cccgtaatag ggaccaagat tgaggacttg
      961 ctgtccatca gctgccctgc ggaactgccg gccgtcccag ccaacagact ctatcccagc
     1021 ggggcctatg acgctttccc gctggccccg ggtgacttag gggagggggc tgagggcctc
     1081 cctgggctcc tgacccctcc tagtggggag ggagggagta gcggcgacgg cggagagttt
     1141 ctggccagta cgcagcctca gctttccccg ctgggccttc gcagcgccgc cgcggcggac
     1201 ttccctaaac ctctggtggc ggacatccct ggaagcagtg gcgtggctgc accacccgtg
     1261 ccgccgccgc cgcccacccc tttcccccag gccaaggcgc gacgcaaggg gcgccgcggc
     1321 ggcaaatgca gcacgcgctg cttctgcccg cggccgcacg ccaaggcctt cgcttgcccg
     1381 gtggagagtt gtgtgcggag ctttgcgcgc tccgacgagc tcaatcgcca cctgcgcatc
     1441 cacacgggcc acaaaccctt ccagtgccgc atctgcctcc gcaacttcag ccgcagcgac
     1501 cacctcacca cgcacgtgcg cacccacacc ggcgagaagc cttttgcttg cgacgtgtgc
     1561 ggccgccgct tcgcgcgcag cgatgagaag aaacggcaca gcaaggtgca cctcaagcag
     1621 aaggcgcgcg ccgaggagcg gctcaagggc ctcggctttt actcgctggg cctctccttc
     1681 gcttctctct gagcaagaga tgggtttatg ggttggggcg ccgccgttcg gcgcgcacga
     1741 gttccgggcc gttcccctcc ccgctcttct tccaactcct cctcgcacgc ccgagggccg
     1801 gcctccggtc ccgcttccag tttccttgaa gcgcccgccg cacacgccct attcagcacc
     1861 agctccgcgg acagttcccg cggtccaggc gctgtcaccc ttgtcagccg cgctttgggg
     1921 gaagtcttct gagaccaccc agtgaatagg cactaccctg ggattcaaga cagtcttttg
     1981 taactggcac acgccccacg ccttcctcta taacccccag agacaggctg gggcagcgcc
     2041 aaggcggtct cgcgcgggac tttgtacagc agtgtcttat ccagcagcca ttggatgtaa
     2101 cgttttgctt tgggtttttt ttccttttgt tgttgttaat ttttgtaaag cagacgctac
     2161 tctcaagcag ttgacaaaac tgtttatttt tgcaattaaa attattgtgc taaaagctta
//
