LOCUS       NM_005228               9905 bp    mRNA    linear   PRI 22-MAR-2025
DEFINITION  Homo sapiens epidermal growth factor receptor (EGFR), transcript
            variant 1, mRNA.
ACCESSION   NM_005228
VERSION     NM_005228.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Wang,X., Men,C., Shan,S., Yang,J., Zhang,S., Ji,X., Li,C. and
            Wang,Y.
  TITLE     EGFR upregulates miRNA subset to inhibit CYBRD1 and cause DDP
            resistance in gastric cancer
  JOURNAL   Gene 933, 149005 (2025)
   PUBMED   39419238
  REMARK    GeneRIF: EGFR upregulates miRNA subset to inhibit CYBRD1 and cause
            DDP resistance in gastric cancer.
REFERENCE   2  (bases 1 to 9905)
  AUTHORS   Li,H., Fang,R., Ma,R., Long,Y., He,R., Lyu,H., Chen,L. and Wen,Y.
  TITLE     Amphiregulin promotes activated regulatory T cell-suppressive
            function via the AREG/EGFR pathway in laryngeal squamous cell
            carcinoma
  JOURNAL   Head Face Med 20 (1), 62 (2024)
   PUBMED   39456084
  REMARK    GeneRIF: Amphiregulin promotes activated regulatory T
            cell-suppressive function via the AREG/EGFR pathway in laryngeal
            squamous cell carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9905)
  AUTHORS   Nie,X.M., Dong,D.F., Lin,J.F., Wu,B.Y. and Cai,G.
  TITLE     [TRAF4 promotes lung cancer development by activating tyrosine
            kinase of EGFR]
  JOURNAL   Zhonghua Zhong Liu Za Zhi 46 (10), 968-978 (2024)
   PUBMED   39414598
  REMARK    GeneRIF: [TRAF4 promotes lung cancer development by activating
            tyrosine kinase of EGFR].
REFERENCE   4  (bases 1 to 9905)
  AUTHORS   Ewoldt,J.K., Wang,M.C., McLellan,M.A., Cloonan,P.E., Chopra,A.,
            Gorham,J., Li,L., DeLaughter,D.M., Gao,X., Lee,J.H.,
            Willcox,J.A.L., Layton,O., Luu,R.J., Toepfer,C.N., Eyckmans,J.,
            Seidman,C.E., Seidman,J.G. and Chen,C.S.
  TITLE     Hypertrophic cardiomyopathy-associated mutations drive stromal
            activation via EGFR-mediated paracrine signaling
  JOURNAL   Sci Adv 10 (42), eadi6927 (2024)
   PUBMED   39413182
  REMARK    GeneRIF: Hypertrophic cardiomyopathy-associated mutations drive
            stromal activation via EGFR-mediated paracrine signaling.
REFERENCE   5  (bases 1 to 9905)
  AUTHORS   Gan,H.K., Cvrljevic,A.N. and Johns,T.G.
  TITLE     The epidermal growth factor receptor variant III (EGFRvIII): where
            wild things are altered
  JOURNAL   FEBS J 280 (21), 5350-5370 (2013)
   PUBMED   23777544
  REMARK    Review article
REFERENCE   6  (bases 1 to 9905)
  AUTHORS   Reiter,J.L., Threadgill,D.W., Eley,G.D., Strunk,K.E.,
            Danielsen,A.J., Sinclair,C.S., Pearsall,R.S., Green,P.J., Yee,D.,
            Lampland,A.L., Balasubramaniam,S., Crossley,T.D., Magnuson,T.R.,
            James,C.D. and Maihle,N.J.
  TITLE     Comparative genomic sequence analysis and isolation of human and
            mouse alternative EGFR transcripts encoding truncated receptor
            isoforms
  JOURNAL   Genomics 71 (1), 1-20 (2001)
   PUBMED   11161793
  REMARK    Erratum:[Genomics. 2010 Aug;96(2):128]
REFERENCE   7  (bases 1 to 9905)
  AUTHORS   Reiter,J.L. and Maihle,N.J.
  TITLE     A 1.8 kb alternative transcript from the human epidermal growth
            factor receptor gene encodes a truncated form of the receptor
  JOURNAL   Nucleic Acids Res 24 (20), 4050-4056 (1996)
   PUBMED   8918811
REFERENCE   8  (bases 1 to 9905)
  AUTHORS   Lowenstein,E.J., Daly,R.J., Batzer,A.G., Li,W., Margolis,B.,
            Lammers,R., Ullrich,A., Skolnik,E.Y., Bar-Sagi,D. and
            Schlessinger,J.
  TITLE     The SH2 and SH3 domain-containing protein GRB2 links receptor
            tyrosine kinases to ras signaling
  JOURNAL   Cell 70 (3), 431-442 (1992)
   PUBMED   1322798
REFERENCE   9  (bases 1 to 9905)
  AUTHORS   Chi,D.D., Hing,A.V., Helms,C., Steinbrueck,T., Mishra,S.K. and
            Donis-Keller,H.
  TITLE     Two chromosome 7 dinucleotide repeat polymorphisms at gene loci
            epidermal growth factor receptor (EGFR) and pro alpha 2 (I)
            collagen (COL1A2)
  JOURNAL   Hum Mol Genet 1 (2), 135 (1992)
   PUBMED   1301150
REFERENCE   10 (bases 1 to 9905)
  AUTHORS   Countaway,J.L., Nairn,A.C. and Davis,R.J.
  TITLE     Mechanism of desensitization of the epidermal growth factor
            receptor protein-tyrosine kinase
  JOURNAL   J Biol Chem 267 (2), 1129-1140 (1992)
   PUBMED   1309762
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC006977.3, BC094761.1,
            HQ912715.1, X00588.1, AF125253.1, AU137334.1, AC073324.6,
            DA371223.1 and AW450657.1.
            
            On Nov 22, 2018 this sequence version replaced NM_005228.4.
            
            Summary: The protein encoded by this gene is a transmembrane
            glycoprotein that is a member of the protein kinase superfamily.
            This protein is a receptor for members of the epidermal growth
            factor family. EGFR is a cell surface protein that binds to
            epidermal growth factor, thus inducing receptor dimerization and
            tyrosine autophosphorylation leading to cell proliferation.
            Mutations in this gene are associated with lung cancer. EGFR is a
            component of the cytokine storm which contributes to a severe form
            of Coronavirus Disease 2019 (COVID-19) resulting from infection
            with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
            [provided by RefSeq, Jul 2020].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X00588.1, SRR1803616.241434.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in cytokine storm inflammatory
                                      response
            MANE Ensembl match     :: ENST00000275493.7/ ENSP00000275493.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 AC006977.3         9124-9127
            5-45                BC094761.1         1-41
            46-112              HQ912715.1         31-97
            113-1880            X00588.1           38-1805
            1881-1890           AF125253.1         1865-1874
            1891-2621           X00588.1           1816-2546
            2622-2631           AU137334.1         238-247
            2632-2969           X00588.1           2557-2894
            2970-2970           AC073324.6         11033-11033
            2971-4188           X00588.1           2896-4113
            4189-5615           HQ912715.1         4045-5471
            5616-6161           DA371223.1         5-550
            6162-6357           AW450657.1         17-212              c
            6358-9905           AC073324.6         20390-23937
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p11.2"
     gene            1..9905
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /note="epidermal growth factor receptor"
                     /db_xref="GeneID:1956"
                     /db_xref="HGNC:HGNC:3236"
                     /db_xref="MIM:131550"
     exon            1..349
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     CDS             262..3894
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /EC_number="2.7.10.1"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; avian erythroblastic leukemia viral (v-erb-b)
                     oncogene homolog; cell proliferation-inducing protein 61;
                     cell growth inhibiting protein 40; proto-oncogene
                     c-ErbB-1; receptor tyrosine-protein kinase erbB-1; erb-b2
                     receptor tyrosine kinase 1; epidermal growth factor
                     receptor tyrosine kinase domain; EGFR vIII"
                     /codon_start=1
                     /product="epidermal growth factor receptor isoform a
                     precursor"
                     /protein_id="NP_005219.2"
                     /db_xref="CCDS:CCDS5514.1"
                     /db_xref="GeneID:1956"
                     /db_xref="HGNC:HGNC:3236"
                     /db_xref="MIM:131550"
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTF
                     EDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLE
                     NLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVE
                     SIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQ
                     CSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQ
                     MDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCR
                     KVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQEL
                     DILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGL
                     RSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHA
                     LCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQA
                     MNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNC
                     TYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRL
                     LQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKI
                     PVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCL
                     LDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDF
                     GLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKP
                     YDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMA
                     RDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTS
                     RTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLP
                     VPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTC
                     VNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQS
                     SEFIGA"
     exon            350..501
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            502..685
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            686..820
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            821..889
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1008
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1150
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1267
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1394
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1468
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1559
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1759
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1892
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            1893..1983
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2141
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            2142..2180
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            2181..2322
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            2323..2445
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            2446..2544
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            2545..2730
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            2731..2886
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            2887..2962
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            2963..3109
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            3110..3207
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            3208..3375
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            3376..3423
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            3424..3532
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     exon            3533..9905
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5589..5594
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /note="hexamer: AATAAA"
     polyA_site      5615
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /note="major polyA site"
     regulatory      9824..9829
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /note="hexamer: ATTAAA"
     polyA_site      9846
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /note="major polyA site"
     regulatory      9883..9888
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
                     /note="hexamer: AATAAT"
     polyA_site      9905
                     /gene="EGFR"
                     /gene_synonym="ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;
                     NNCIS; PIG61"
ORIGIN      
        1 agacgtccgg gcagcccccg gcgcagcgcg gccgcagcag cctccgcccc ccgcacggtg
       61 tgagcgcccg acgcggccga ggcggccgga gtcccgagct agccccggcg gccgccgccg
      121 cccagaccgg acgacaggcc acctcgtcgg cgtccgcccg agtccccgcc tcgccgccaa
      181 cgccacaacc accgcgcacg gccccctgac tccgtccagt attgatcggg agagccggag
      241 cgagctcttc ggggagcagc gatgcgaccc tccgggacgg ccggggcagc gctcctggcg
      301 ctgctggctg cgctctgccc ggcgagtcgg gctctggagg aaaagaaagt ttgccaaggc
      361 acgagtaaca agctcacgca gttgggcact tttgaagatc attttctcag cctccagagg
      421 atgttcaata actgtgaggt ggtccttggg aatttggaaa ttacctatgt gcagaggaat
      481 tatgatcttt ccttcttaaa gaccatccag gaggtggctg gttatgtcct cattgccctc
      541 aacacagtgg agcgaattcc tttggaaaac ctgcagatca tcagaggaaa tatgtactac
      601 gaaaattcct atgccttagc agtcttatct aactatgatg caaataaaac cggactgaag
      661 gagctgccca tgagaaattt acaggaaatc ctgcatggcg ccgtgcggtt cagcaacaac
      721 cctgccctgt gcaacgtgga gagcatccag tggcgggaca tagtcagcag tgactttctc
      781 agcaacatgt cgatggactt ccagaaccac ctgggcagct gccaaaagtg tgatccaagc
      841 tgtcccaatg ggagctgctg gggtgcagga gaggagaact gccagaaact gaccaaaatc
      901 atctgtgccc agcagtgctc cgggcgctgc cgtggcaagt cccccagtga ctgctgccac
      961 aaccagtgtg ctgcaggctg cacaggcccc cgggagagcg actgcctggt ctgccgcaaa
     1021 ttccgagacg aagccacgtg caaggacacc tgccccccac tcatgctcta caaccccacc
     1081 acgtaccaga tggatgtgaa ccccgagggc aaatacagct ttggtgccac ctgcgtgaag
     1141 aagtgtcccc gtaattatgt ggtgacagat cacggctcgt gcgtccgagc ctgtggggcc
     1201 gacagctatg agatggagga agacggcgtc cgcaagtgta agaagtgcga agggccttgc
     1261 cgcaaagtgt gtaacggaat aggtattggt gaatttaaag actcactctc cataaatgct
     1321 acgaatatta aacacttcaa aaactgcacc tccatcagtg gcgatctcca catcctgccg
     1381 gtggcattta ggggtgactc cttcacacat actcctcctc tggatccaca ggaactggat
     1441 attctgaaaa ccgtaaagga aatcacaggg tttttgctga ttcaggcttg gcctgaaaac
     1501 aggacggacc tccatgcctt tgagaaccta gaaatcatac gcggcaggac caagcaacat
     1561 ggtcagtttt ctcttgcagt cgtcagcctg aacataacat ccttgggatt acgctccctc
     1621 aaggagataa gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat
     1681 acaataaact ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat tataagcaac
     1741 agaggtgaaa acagctgcaa ggccacaggc caggtctgcc atgccttgtg ctcccccgag
     1801 ggctgctggg gcccggagcc cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg
     1861 gaatgcgtgg acaagtgcaa ccttctggag ggtgagccaa gggagtttgt ggagaactct
     1921 gagtgcatac agtgccaccc agagtgcctg cctcaggcca tgaacatcac ctgcacagga
     1981 cggggaccag acaactgtat ccagtgtgcc cactacattg acggccccca ctgcgtcaag
     2041 acctgcccgg caggagtcat gggagaaaac aacaccctgg tctggaagta cgcagacgcc
     2101 ggccatgtgt gccacctgtg ccatccaaac tgcacctacg gatgcactgg gccaggtctt
     2161 gaaggctgtc caacgaatgg gcctaagatc ccgtccatcg ccactgggat ggtgggggcc
     2221 ctcctcttgc tgctggtggt ggccctgggg atcggcctct tcatgcgaag gcgccacatc
     2281 gttcggaagc gcacgctgcg gaggctgctg caggagaggg agcttgtgga gcctcttaca
     2341 cccagtggag aagctcccaa ccaagctctc ttgaggatct tgaaggaaac tgaattcaaa
     2401 aagatcaaag tgctgggctc cggtgcgttc ggcacggtgt ataagggact ctggatccca
     2461 gaaggtgaga aagttaaaat tcccgtcgct atcaaggaat taagagaagc aacatctccg
     2521 aaagccaaca aggaaatcct cgatgaagcc tacgtgatgg ccagcgtgga caacccccac
     2581 gtgtgccgcc tgctgggcat ctgcctcacc tccaccgtgc agctcatcac gcagctcatg
     2641 cccttcggct gcctcctgga ctatgtccgg gaacacaaag acaatattgg ctcccagtac
     2701 ctgctcaact ggtgtgtgca gatcgcaaag ggcatgaact acttggagga ccgtcgcttg
     2761 gtgcaccgcg acctggcagc caggaacgta ctggtgaaaa caccgcagca tgtcaagatc
     2821 acagattttg ggctggccaa actgctgggt gcggaagaga aagaatacca tgcagaagga
     2881 ggcaaagtgc ctatcaagtg gatggcattg gaatcaattt tacacagaat ctatacccac
     2941 cagagtgatg tctggagcta cggggtgact gtttgggagt tgatgacctt tggatccaag
     3001 ccatatgacg gaatccctgc cagcgagatc tcctccatcc tggagaaagg agaacgcctc
     3061 cctcagccac ccatatgtac catcgatgtc tacatgatca tggtcaagtg ctggatgata
     3121 gacgcagata gtcgcccaaa gttccgtgag ttgatcatcg aattctccaa aatggcccga
     3181 gacccccagc gctaccttgt cattcagggg gatgaaagaa tgcatttgcc aagtcctaca
     3241 gactccaact tctaccgtgc cctgatggat gaagaagaca tggacgacgt ggtggatgcc
     3301 gacgagtacc tcatcccaca gcagggcttc ttcagcagcc cctccacgtc acggactccc
     3361 ctcctgagct ctctgagtgc aaccagcaac aattccaccg tggcttgcat tgatagaaat
     3421 gggctgcaaa gctgtcccat caaggaagac agcttcttgc agcgatacag ctcagacccc
     3481 acaggcgcct tgactgagga cagcatagac gacaccttcc tcccagtgcc tgaatacata
     3541 aaccagtccg ttcccaaaag gcccgctggc tctgtgcaga atcctgtcta tcacaatcag
     3601 cctctgaacc ccgcgcccag cagagaccca cactaccagg acccccacag cactgcagtg
     3661 ggcaaccccg agtatctcaa cactgtccag cccacctgtg tcaacagcac attcgacagc
     3721 cctgcccact gggcccagaa aggcagccac caaattagcc tggacaaccc tgactaccag
     3781 caggacttct ttcccaagga agccaagcca aatggcatct ttaagggctc cacagctgaa
     3841 aatgcagaat acctaagggt cgcgccacaa agcagtgaat ttattggagc atgaccacgg
     3901 aggatagtat gagccctaaa aatccagact ctttcgatac ccaggaccaa gccacagcag
     3961 gtcctccatc ccaacagcca tgcccgcatt agctcttaga cccacagact ggttttgcaa
     4021 cgtttacacc gactagccag gaagtacttc cacctcgggc acattttggg aagttgcatt
     4081 cctttgtctt caaactgtga agcatttaca gaaacgcatc cagcaagaat attgtccctt
     4141 tgagcagaaa tttatctttc aaagaggtat atttgaaaaa aaaaaaaagt atatgtgagg
     4201 atttttattg attggggatc ttggagtttt tcattgtcgc tattgatttt tacttcaatg
     4261 ggctcttcca acaaggaaga agcttgctgg tagcacttgc taccctgagt tcatccaggc
     4321 ccaactgtga gcaaggagca caagccacaa gtcttccaga ggatgcttga ttccagtggt
     4381 tctgcttcaa ggcttccact gcaaaacact aaagatccaa gaaggccttc atggccccag
     4441 caggccggat cggtactgta tcaagtcatg gcaggtacag taggataagc cactctgtcc
     4501 cttcctgggc aaagaagaaa cggaggggat ggaattcttc cttagactta cttttgtaaa
     4561 aatgtcccca cggtacttac tccccactga tggaccagtg gtttccagtc atgagcgtta
     4621 gactgacttg tttgtcttcc attccattgt tttgaaactc agtatgctgc ccctgtcttg
     4681 ctgtcatgaa atcagcaaga gaggatgaca catcaaataa taactcggat tccagcccac
     4741 attggattca tcagcatttg gaccaatagc ccacagctga gaatgtggaa tacctaagga
     4801 tagcaccgct tttgttctcg caaaaacgta tctcctaatt tgaggctcag atgaaatgca
     4861 tcaggtcctt tggggcatag atcagaagac tacaaaaatg aagctgctct gaaatctcct
     4921 ttagccatca ccccaacccc ccaaaattag tttgtgttac ttatggaaga tagttttctc
     4981 cttttacttc acttcaaaag ctttttactc aaagagtata tgttccctcc aggtcagctg
     5041 cccccaaacc ccctccttac gctttgtcac acaaaaagtg tctctgcctt gagtcatcta
     5101 ttcaagcact tacagctctg gccacaacag ggcattttac aggtgcgaat gacagtagca
     5161 ttatgagtag tgtggaattc aggtagtaaa tatgaaacta gggtttgaaa ttgataatgc
     5221 tttcacaaca tttgcagatg ttttagaagg aaaaaagttc cttcctaaaa taatttctct
     5281 acaattggaa gattggaaga ttcagctagt taggagccca ccttttttcc taatctgtgt
     5341 gtgccctgta acctgactgg ttaacagcag tcctttgtaa acagtgtttt aaactctcct
     5401 agtcaatatc caccccatcc aatttatcaa ggaagaaatg gttcagaaaa tattttcagc
     5461 ctacagttat gttcagtcac acacacatac aaaatgttcc ttttgctttt aaagtaattt
     5521 ttgactccca gatcagtcag agcccctaca gcattgttaa gaaagtattt gatttttgtc
     5581 tcaatgaaaa taaaactata ttcatttcca ctctattatg ctctcaaata cccctaagca
     5641 tctatactag cctggtatgg gtatgaaaga tacaaagata aataaaacat agtccctgat
     5701 tctaagaaat tcacaattta gcaaaggaaa tggactcata gatgctaacc ttaaaacaac
     5761 gtgacaaatg ccagacagga cccatcagcc aggcactgtg agagcacaga gcagggaggt
     5821 tgggtcctgc ctgaggagac ctggaaggga ggcctcacag gaggatgacc aggtctcagt
     5881 cagcggggag gtggaaagtg caggtgcatc aggggcaccc tgaccgagga aacagctgcc
     5941 agaggcctcc actgctaaag tccacataag gctgaggtca gtcaccctaa acaacctgct
     6001 ccctctaagc caggggatga gcttggagca tcccacaagt tccctaaaag ttgcagcccc
     6061 cagggggatt ttgagctatc atctctgcac atgcttagtg agaagactac acaacatttc
     6121 taagaatctg agattttata ttgtcagtta accactttca ttattcattc acctcaggac
     6181 atgcagaaat atttcagtca gaactgggaa acagaaggac ctacattctg ctgtcactta
     6241 tgtgtcaaga agcagatgat cgatgaggca ggtcagttgt aagtgagtca cattgtagca
     6301 ttaaattcta gtatttttgt agtttgaaac agtaacttaa taaaagagca aaagctattc
     6361 tagctttctt cttcatattt taattttcca ccataaagtt tagttgctaa attctattaa
     6421 ttttaagatt gtgcttccca aaatagttct cacttcatct gtccagggag gcacagttct
     6481 gtctggtaga agccgcaaag cccttagcct cttcacggat ctggcgactg tgatgggcag
     6541 gtcaggagag gagctgccca aagtcccatg attttcacct aacagccctg atcagtcagt
     6601 actcaaagct tggactccat ccctgaaggt cttcctgatt gatagcctgg ccttaatacc
     6661 ctacagaaag cctgtccatt ggctgtttct tcctcagtca gttcctggaa gaccttaccc
     6721 catgacccca gcttcagatg tggtctttgg aaacagaggt cgaaggaaag taaggagctg
     6781 agagctcaca ttcataggtg ccgccagcct tcgtgcatct tcttgcatca tctctaagga
     6841 gctcctctaa ttacaccatg cccgtcaccc catgagggat cagagaaggg atgagtcttc
     6901 taaactctat attcgctgtg agtccaggtt gtaaggggga gcactgtgga tgcatcctat
     6961 tgcactccag ctgatgacac caaagcttag gtgtttgctg aaagttcttg atgttgtgac
     7021 ttaccacccc tgcctcacaa ctgcagacat aaggggacta tggattgctt agcaggaaag
     7081 gcactggttc tcaagggcgg ctgcccttgg gaatcttctg gtcccaacca gaaagactgt
     7141 ggcttgattt tctcaggtgc agcccagccg tagggccttt tcagagcacc ccctggttat
     7201 tgcaacattc atcaaagttt ctagaacctc tggcctaaag gaagggcctg gtgggatcta
     7261 cttggcactc gctggggggc caccccccag tgccactctc actaggcctc tgattgcact
     7321 tgtgtaggat gaagctggtg ggtgatggga actcagcacc tcccctcagg cagaaaagaa
     7381 tcatctgtgg agcttcaaaa gaaggggcct ggagtctctg cagaccaatt caacccaaat
     7441 ctcgggggct ctttcatgat tctaatgggc aaccagggtt gaaaccctta tttctagggt
     7501 cttcagttgt acaagactgt gggtctgtac cagagccccc gtcagagtag aataaaaggc
     7561 tgggtagggt agagattccc atgtgcagtg gagagaacaa tctgcagtca ctgataagcc
     7621 tgagacttgg ctcatttcaa aagcgttcaa ttcatcctca ccagcagttc agctggaaag
     7681 gggcaaatac ccccacctga gctttgaaaa cgccctggga ccctctgcat tctctaagta
     7741 agttatagaa accagtctct tccctccttt gtgagtgagc tgctattcca cgtaggcaac
     7801 acctgttgaa attgccctca atgtctactc tgcatttctt tcttgtgata agcacacact
     7861 tttattgcaa cataatgatc tgctcacatt tccttgcctg ggggctgtaa aaccttacag
     7921 aacagaaatc cttgcctctt tcaccagcca cacctgccat accaggggta cagctttgta
     7981 ctattgaaga cacagacagg atttttaaat gtaaatctat ttttgtaact ttgttgcggg
     8041 atatagttct ctttatgtag cactgaactt tgtacaatat atttttagaa actcattttt
     8101 ctactaaaac aaacacagtt tactttagag agactgcaat agaatcaaaa tttgaaactg
     8161 aaatctttgt ttaaaagggt taagttgagg caagaggaaa gccctttctc tctcttataa
     8221 aaaggcacaa cctcattggg gagctaagct aggtcattgt catggtgaag aagagaagca
     8281 tcgtttttat atttaggaaa ttttaaaaga tgatggaaag cacatttagc ttggtctgag
     8341 gcaggttctg ttggggcagt gttaatggaa agggctcact gttgttacta ctagaaaaat
     8401 ccagttgcat gccatactct catcatctgc cagtgtaacc ctgtacatgt aagaaaagca
     8461 ataacatagc actttgttgg tttatatata taatgtgact tcaatgcaaa ttttattttt
     8521 atatttacaa ttgatatgca tttaccagta taaactagac atgtctggag agcctaataa
     8581 tgttcagcac actttggtta gttcaccaac agtcttacca agcctgggcc cagccaccct
     8641 agagaagtta ttcagccctg gctgcagtga catcacctga ggagctttta aaagcttgaa
     8701 gcccagctac acctcagacc gattaaacgc aaatctctgg ggctgaaacc caagcattcg
     8761 tagtttttaa agctcctgag gtcattccaa tgtgcggcca aagttgagaa ctactggcct
     8821 agggattagc cacaaggaca tggacttgga ggcaaattct gcaggtgtat gtgattctca
     8881 ggcctagaga gctaagacac aaagacctcc acatctgtcg ctgagagtca agaacctgaa
     8941 cagagtttcc atgaaggttc tccaagcact agaagggaga gtgtctaaac aatggttgaa
     9001 aagcaaagga aatataaaac agacacctct ttccatttcc taaggtttct ctctttatta
     9061 agggtggact agtaataaaa tataatattc ttgctgctta tgcagctgac attgttgccc
     9121 tccctaaagc aaccaagtag cctttatttc ccacagtgaa agaaaacgct ggcctatcag
     9181 ttacattaca aaaggcagat ttcaagagga ttgagtaagt agttggatgg ctttcataaa
     9241 aacaagaatt caagaagagg attcatgctt taagaaacat ttgttataca ttcctcacaa
     9301 attatacctg ggataaaaac tatgtagcag gcagtgtgtt ttccttccat gtctctctgc
     9361 actacctgca gtgtgtcctc tgaggctgca agtctgtcct atctgaattc ccagcagaag
     9421 cactaagaag ctccacccta tcacctagca gataaaacta tggggaaaac ttaaatctgt
     9481 gcatacattt ctggatgcat ttacttatct ttaaaaaaaa aggaatccta tgacctgatt
     9541 tggccacaaa aataatcttg ctgtacaata caatctcttg gaaattaaga gatcctatgg
     9601 atttgatgac tggtattaga ggtgacaatg taaccgatta acaacagaca gcaataactt
     9661 cgttttagaa acattcaagc aatagcttta tagcttcaac atatggtacg ttttaacctt
     9721 gaaagttttg caatgatgaa agcagtattt gtacaaatga aaagcagaat tctcttttat
     9781 atggtttata ctgttgatca gaaatgttga ttgtgcattg agtattaaaa aattagatgt
     9841 atattattca ttgttcttta ctcctgagta ccttataata ataataatgt attctttgtt
     9901 aacaa
//
