LOCUS       NM_001178130            6265 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens epidermal growth factor (EGF), transcript variant 2,
            mRNA.
ACCESSION   NM_001178130
VERSION     NM_001178130.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Chatterjee,P., Ghosh,D., Chowdhury,S.R. and Roy,S.S.
  TITLE     ETS1 drives EGF-induced glycolytic shift and metastasis of
            epithelial ovarian cancer cells
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1871 (8), 119805 (2024)
   PUBMED   39159682
  REMARK    GeneRIF: ETS1 drives EGF-induced glycolytic shift and metastasis of
            epithelial ovarian cancer cells.
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Hu,S., Yuan,Y., Yan,R., Xie,H., Cai,R. and Xu,J.
  TITLE     NRF2 regulates EGF stability through OTUD4 in lung adenocarcinoma
  JOURNAL   Biochem Biophys Res Commun 708, 149798 (2024)
   PUBMED   38518721
  REMARK    GeneRIF: NRF2 regulates EGF stability through OTUD4 in lung
            adenocarcinoma.
REFERENCE   3  (bases 1 to 6265)
  AUTHORS   Czerczak-Kwiatkowska,K., Kaminska,M., Fraczyk,J., Majsterek,I. and
            Kolesinska,B.
  TITLE     Searching for EGF Fragments Recreating the Outer Sphere of the
            Growth Factor Involved in Receptor Interactions
  JOURNAL   Int J Mol Sci 25 (3), 1470 (2024)
   PUBMED   38338748
  REMARK    GeneRIF: Searching for EGF Fragments Recreating the Outer Sphere of
            the Growth Factor Involved in Receptor Interactions.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6265)
  AUTHORS   Hommel,U., Harvey,T.S., Driscoll,P.C. and Campbell,I.D.
  TITLE     Human epidermal growth factor. High resolution solution structure
            and comparison with human transforming growth factor alpha
  JOURNAL   J Mol Biol 227 (1), 271-282 (1992)
   PUBMED   1522591
REFERENCE   5  (bases 1 to 6265)
  AUTHORS   Hernandez-Sotomayor,S.M. and Carpenter,G.
  TITLE     Epidermal growth factor receptor: elements of intracellular
            communication
  JOURNAL   J Membr Biol 128 (2), 81-89 (1992)
   PUBMED   1501243
  REMARK    Review article
REFERENCE   6  (bases 1 to 6265)
  AUTHORS   Bell,G.I., Fong,N.M., Stempien,M.M., Wormsted,M.A., Caput,D.,
            Ku,L.L., Urdea,M.S., Rall,L.B. and Sanchez-Pescador,R.
  TITLE     Human epidermal growth factor precursor: cDNA sequence, expression
            in vitro and gene organization
  JOURNAL   Nucleic Acids Res 14 (21), 8427-8446 (1986)
   PUBMED   3491360
REFERENCE   7  (bases 1 to 6265)
  AUTHORS   Morton,C.C., Byers,M.G., Nakai,H., Bell,G.I. and Shows,T.B.
  TITLE     Human genes for insulin-like growth factors I and II and epidermal
            growth factor are located on 12q22----q24.1, 11p15, and
            4q25----q27, respectively
  JOURNAL   Cytogenet Cell Genet 41 (4), 245-249 (1986)
   PUBMED   3486749
REFERENCE   8  (bases 1 to 6265)
  AUTHORS   Brissenden,J.E., Ullrich,A. and Francke,U.
  TITLE     Human chromosomal mapping of genes for insulin-like growth factors
            I and II and epidermal growth factor
  JOURNAL   Nature 310 (5980), 781-784 (1984)
   PUBMED   6382023
REFERENCE   9  (bases 1 to 6265)
  AUTHORS   Smith,J., Cook,E., Fotheringham,I., Pheby,S., Derbyshire,R.,
            Eaton,M.A., Doel,M., Lilley,D.M., Pardon,J.F., Patel,T., Lewis,H.
            and Bell,L.D.
  TITLE     Chemical synthesis and cloning of a gene for human beta-urogastrone
  JOURNAL   Nucleic Acids Res 10 (15), 4467-4482 (1982)
   PUBMED   6290982
REFERENCE   10 (bases 1 to 6265)
  AUTHORS   Gregory,H. and Preston,B.M.
  TITLE     The primary structure of human urogastrone
  JOURNAL   Int J Pept Protein Res 9 (2), 107-118 (1977)
   PUBMED   300079
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005509.1, BC143357.1,
            AC004050.1 and CA437074.1.
            
            On May 31, 2019 this sequence version replaced NM_001178130.2.
            
            Summary: This gene encodes a member of the epidermal growth factor
            superfamily. The encoded preproprotein is proteolytically processed
            to generate the 53-amino acid epidermal growth factor peptide. This
            protein acts a potent mitogenic factor that plays an important role
            in the growth, proliferation and differentiation of numerous cell
            types. This protein acts by binding with high affinity to the cell
            surface receptor, epidermal growth factor receptor. Defects in this
            gene are the cause of hypomagnesemia type 4. Dysregulation of this
            gene has been associated with the growth and progression of certain
            cancers. Alternative splicing results in multiple transcript
            variants, at least one of which encodes a preproprotein that is
            proteolytically processed. [provided by RefSeq, Jan 2016].
            
            Transcript Variant: This variant (2) lacks an in-frame exon in the
            coding region, compared to variant 1. The encoded isoform (2) is
            shorter than isoform 1. This isoform (2) may undergo proteolytic
            processing similar to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC143357.1 [ECO:0000332]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-358               AC005509.1         64439-64796
            359-2576            BC143357.1         1-2218
            2577-2577           AC005509.1         131598-131598
            2578-3169           BC143357.1         2220-2811
            3170-4000           BC143357.1         2813-3643
            4001-4867           AC004050.1         102948-103814       c
            4868-5461           CA437074.1         18-611              c
            5462-6265           AC004050.1         101550-102353       c
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q25"
     gene            1..6265
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /note="epidermal growth factor"
                     /db_xref="GeneID:1950"
                     /db_xref="HGNC:HGNC:3229"
                     /db_xref="MIM:131530"
     exon            1..580
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     CDS             454..3954
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; beta-urogastrone; pro-epidermal growth factor"
                     /codon_start=1
                     /product="pro-epidermal growth factor isoform 2 precursor"
                     /protein_id="NP_001171601.1"
                     /db_xref="CCDS:CCDS54794.1"
                     /db_xref="GeneID:1950"
                     /db_xref="HGNC:HGNC:3229"
                     /db_xref="MIM:131530"
                     /translation="MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGP
                     APFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVF
                     LNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYP
                     ANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQ
                     YNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHT
                     GKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDL
                     QSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDG
                     KRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQL
                     CVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLS
                     QQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIG
                     RRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIE
                     SSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQN
                     DVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKP
                     GADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKN
                     QVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQ
                     CLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDST
                     PPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQ
                     YRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPY
                     EESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGSMQPT
                     SWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLS
                     ANPLWQQRALDPPHQMELTQ"
     exon            581..780
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            781..962
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1190
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1393
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1519
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1642
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1765
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            1766..1891
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            1892..2028
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2177
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            2178..2282
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            2283..2506
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            2507..2674
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            2675..2824
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            2825..2944
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            2945..3061
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            3062..3187
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            3188..3335
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            3336..3503
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            3504..3621
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            3622..3700
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     exon            3701..6265
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4743..4748
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /note="hexamer: AATAAA"
     polyA_site      4765
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /note="major polyA site"
     regulatory      5442..5447
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /note="hexamer: AATAAA"
     polyA_site      5461
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
     regulatory      6244..6249
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
                     /note="hexamer: AATAAA"
     polyA_site      6265
                     /gene="EGF"
                     /gene_synonym="HOMG4; URG"
ORIGIN      
        1 caaaaagaga aactgttggg agaggaatcg tatctccata tttcttcttt cagccccaat
       61 ccaagggttg tagctggaac tttccatcag ttcttccttt ctttttcctc tctaagcctt
      121 tgccttgctc tgtcacagtg aagtcagcca gagcagggct gttaaactct gtgaaatttg
      181 tcataagggt gtcaggtatt tcttactggc ttccaaagaa acatagataa agaaatcttt
      241 cctgtggctt cccttggcag gctgcattca gaaggtctct cagttgaaga aagagcttgg
      301 aggacaacag cacaacagga gagtaaaaga tgccccaggg ctgaggcctc cgctcaggca
      361 gccgcatctg gggtcaatca tactcacctt gcccgggcca tgctccagca aaatcaagct
      421 gttttctttt gaaagttcaa actcatcaag attatgctgc tcactcttat cattctgttg
      481 ccagtagttt caaaatttag ttttgttagt ctctcagcac cgcagcactg gagctgtcct
      541 gaaggtactc tcgcaggaaa tgggaattct acttgtgtgg gtcctgcacc cttcttaatt
      601 ttctcccatg gaaatagtat ctttaggatt gacacagaag gaaccaatta tgagcaattg
      661 gtggtggatg ctggtgtctc agtgatcatg gattttcatt ataatgagaa aagaatctat
      721 tgggtggatt tagaaagaca acttttgcaa agagtttttc tgaatgggtc aaggcaagag
      781 agagtatgta atatagagaa aaatgtttct ggaatggcaa taaattggat aaatgaagaa
      841 gttatttggt caaatcaaca ggaaggaatc attacagtaa cagatatgaa aggaaataat
      901 tcccacattc ttttaagtgc tttaaaatat cctgcaaatg tagcagttga tccagtagaa
      961 aggtttatat tttggtcttc agaggtggct ggaagccttt atagagcaga tctcgatggt
     1021 gtgggagtga aggctctgtt ggagacatca gagaaaataa cagctgtgtc attggatgtg
     1081 cttgataagc ggctgttttg gattcagtac aacagagaag gaagcaattc tcttatttgc
     1141 tcctgtgatt atgatggagg ttctgtccac attagtaaac atccaacaca gcataatttg
     1201 tttgcaatgt ccctttttgg tgaccgtatc ttctattcaa catggaaaat gaagacaatt
     1261 tggatagcca acaaacacac tggaaaggac atggttagaa ttaacctcca ttcatcattt
     1321 gtaccacttg gtgaactgaa agtagtgcat ccacttgcac aacccaaggc agaagatgac
     1381 acttgggagc ctgagcagaa actttgcaaa ttgaggaaag gaaactgcag cagcactgtg
     1441 tgtgggcaag acctccagtc acacttgtgc atgtgtgcag agggatacgc cctaagtcga
     1501 gaccggaagt actgtgaaga tgttaatgaa tgtgcttttt ggaatcatgg ctgtactctt
     1561 gggtgtaaaa acacccctgg atcctattac tgcacgtgcc ctgtaggatt tgttctgctt
     1621 cctgatggga aacgatgtca tcaacttgtt tcctgtccac gcaatgtgtc tgaatgcagc
     1681 catgactgtg ttctgacatc agaaggtccc ttatgtttct gtcctgaagg ctcagtgctt
     1741 gagagagatg ggaaaacatg tagcggttgt tcctcacccg ataatggtgg atgtagccag
     1801 ctctgcgttc ctcttagccc agtatcctgg gaatgtgatt gctttcctgg gtatgaccta
     1861 caactggatg aaaaaagctg tgcagcttca ggaccacaac catttttgct gtttgccaat
     1921 tctcaagata ttcgacacat gcattttgat ggaacagact atggaactct gctcagccag
     1981 cagatgggaa tggtttatgc cctagatcat gaccctgtgg aaaataagat atactttgcc
     2041 catacagccc tgaagtggat agagagagct aatatggatg gttcccagcg agaaaggctt
     2101 attgaggaag gagtagatgt gccagaaggt cttgctgtgg actggattgg ccgtagattc
     2161 tattggacag acagagggaa atctctgatt ggaaggagtg atttaaatgg gaaacgttcc
     2221 aaaataatca ctaaggagaa catctctcaa ccacgaggaa ttgctgttca tccaatggcc
     2281 aagagattat tctggactga tacagggatt aatccacgaa ttgaaagttc ttccctccaa
     2341 ggccttggcc gtctggttat agccagctct gatctaatct ggcccagtgg aataacgatt
     2401 gacttcttaa ctgacaagtt gtactggtgc gatgccaagc agtctgtgat tgaaatggcc
     2461 aatctggatg gttcaaaacg ccgaagactt acccagaatg atgtaggtca cccatttgct
     2521 gtagcagtgt ttgaggatta tgtgtggttc tcagattggg ctatgccatc agtaatgaga
     2581 gtaaacaaga ggactggcaa agatagagta cgtctccaag gcagcatgct gaagccctca
     2641 tcactggttg tggttcatcc attggcaaaa ccaggagcag atccctgctt atatcaaaac
     2701 ggaggctgtg aacatatttg caaaaagagg cttggaactg cttggtgttc gtgtcgtgaa
     2761 ggttttatga aagcctcaga tgggaaaacg tgtctggctc tggatggtca tcagctgttg
     2821 gcaggtggtg aagttgatct aaagaaccaa gtaacaccat tggacatctt gtccaagact
     2881 agagtgtcag aagataacat tacagaatct caacacatgc tagtggctga aatcatggtg
     2941 tcagatcaag atgactgtgc tcctgtggga tgcagcatgt atgctcggtg tatttcagag
     3001 ggagaggatg ccacatgtca gtgtttgaaa ggatttgctg gggatggaaa actatgttct
     3061 gatatagatg aatgtgagat gggtgtccca gtgtgccccc ctgcctcctc caagtgcatc
     3121 aacaccgaag gtggttatgt ctgccggtgc tcagaaggct accaaggaga tgggattcac
     3181 tgtcttgact ctactccacc ccctcacctc agggaagatg accaccacta ttccgtaaga
     3241 aatagtgact ctgaatgtcc cctgtcccac gatgggtact gcctccatga tggtgtgtgc
     3301 atgtatattg aagcattgga caagtatgca tgcaactgtg ttgttggcta catcggggag
     3361 cgatgtcagt accgagacct gaagtggtgg gaactgcgcc acgctggcca cgggcagcag
     3421 cagaaggtca tcgtggtggc tgtctgcgtg gtggtgcttg tcatgctgct cctcctgagc
     3481 ctgtgggggg cccactacta caggactcag aagctgctat cgaaaaaccc aaagaatcct
     3541 tatgaggagt cgagcagaga tgtgaggagt cgcaggcctg ctgacactga ggatgggatg
     3601 tcctcttgcc ctcaaccttg gtttgtggtt ataaaagaac accaagacct caagaatggg
     3661 ggtcaaccag tggctggtga ggatggccag gcagcagatg ggtcaatgca accaacttca
     3721 tggaggcagg agccccagtt atgtggaatg ggcacagagc aaggctgctg gattccagta
     3781 tccagtgata agggctcctg tccccaggta atggagcgaa gctttcatat gccctcctat
     3841 gggacacaga cccttgaagg gggtgtcgag aagccccatt ctctcctatc agctaaccca
     3901 ttatggcaac aaagggccct ggacccacca caccaaatgg agctgactca gtgaaaactg
     3961 gaattaaaag gaaagtcaag aagaatgaac tatgtcgatg cacagtatct tttctttcaa
     4021 aagtagagca aaactatagg ttttggttcc acaatctcta cgactaatca cctactcaat
     4081 gcctggagac agatacgtag ttgtgctttt gtttgctctt ttaagcagtc tcactgcagt
     4141 cttatttcca agtaagagta ctgggagaat cactaggtaa cttattagaa acccaaattg
     4201 ggacaacagt gctttgtaaa ttgtgttgtc ttcagcagtc aatacaaata gatttttgtt
     4261 tttgttgttc ctgcagcccc agaagaaatt aggggttaaa gcagacagtc acactggttt
     4321 ggtcagttac aaagtaattt ctttgatctg gacagaacat ttatatcagt ttcatgaaat
     4381 gattggaata ttacaatacc gttaagatac agtgtaggca tttaactcct cattggcgtg
     4441 gtccatgctg atgattttgc aaaatgagtt gtgatgaatc aatgaaaaat gtaatttaga
     4501 aactgatttc ttcagaatta gatggcttat tttttaaaat atttgaatga aaacatttta
     4561 tttttaaaat attacacagg aggcttcgga gtttcttagt cattactgtc cttttcccct
     4621 acagaatttt ccctcttggt gtgattgcac agaatttgta tgtattttca gttacaagat
     4681 tgtaagtaaa ttgcctgatt tgttttcatt atagacaacg atgaatttct tctaattatt
     4741 taaataaaat caccaaaaac ataaacattt tattgtatgc ctgattaagt agttaattat
     4801 agtctaaggc agtactagag ttgaaccaaa atgatttgtc aagcttgctg atgtttctgt
     4861 ttttcgtttt tttttttttt ccggagagag gataggatct cactctgtta tccaggctgg
     4921 agtgtgcaat ggcacaatca tagctcagtg cagcctcaaa ctcctgggct caagcaatcc
     4981 tcctgcctca gcctcccgag taactaggac cacaggcaca ggccaccatg cctggctaag
     5041 gtttttattt ttattttttg tagacatggg gatcacacaa tgttgcccag gctggtcttg
     5101 aactcctggc ctcaagcaag gtcgtgctgg taattttgca aaatgaattg tgattgactt
     5161 tcagcctccc aacgtattag attataggca ttagccatgg tgcccagcct tgtaactttt
     5221 aaaaaaattt tttaatctac aactctgtag attaaaattt cacatggtgt tctaattaaa
     5281 tatttttctt gcagccaaga tattgttact acagataaca caacctgata tggtaacttt
     5341 aaattttggg ggctttgaat cattcagttt atgcattaac tagtcccttt gtttatcttt
     5401 catttctcaa ccccttgtac tttggtgata ccagacatca gaataaaaag aaattgaagt
     5461 acctgttttc aaatggatac tttataggaa ttttggtaaa gatttggtga tgggaggatg
     5521 acttgaggtt tgtggatatt agttaattat tcagtatgat acctcaccca gctaatttag
     5581 atttttctat attcggtttt gctttcattg acaatatcct ggaggatcag aagacttgtc
     5641 tatttctgct gagtcactgg cctcagaaaa ataataacca taatttcccc caaggttttc
     5701 tttacctaag tgtgaatatt ttttcttcct ccaaaagctc acttttgggt ttagattaaa
     5761 tttttgtatt ttagcacctt tttcttttag gggttcaatg atgacaaaag aaatgacatg
     5821 agaacacggc tacccataac ataccattat ctttgtacca gaaaaatcct tgtttccttc
     5881 ttaatgactc tggtacctta gaaactggga ccctgctaag tccttgacta ggctatctac
     5941 cagctcctgg tcggattaaa gaaaaaacac actttgtgtt ttttaatcac caaggcaccc
     6001 tgcagagata tcttcttctt gcaacttcac atctttatca gtaatgtcct ctttccttta
     6061 aaaattcaag ttttaagaac agcattttca tgtaaaaact tgatttgtgt tttttccaga
     6121 ctgaatactt ttcctcccta actctcatcg tctcattgcg cgcaacgcct gattgagctt
     6181 ctgtttgact aaatatcacc tactatgtaa aaaatgagca tattggcctc ttttctagca
     6241 tctaataaag gcttaataca ctgta
//
