LOCUS       NM_001962               5372 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ephrin A5 (EFNA5), transcript variant 1, mRNA.
ACCESSION   NM_001962
VERSION     NM_001962.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Jukonen,J., Moyano-Galceran,L., Hopfner,K., Pietila,E.A.,
            Lehtinen,L., Huhtinen,K., Gucciardo,E., Hynninen,J., Hietanen,S.,
            Grenman,S., Ojala,P.M., Carpen,O. and Lehti,K.
  TITLE     Aggressive and recurrent ovarian cancers upregulate ephrinA5, a
            non-canonical effector of EphA2 signaling duality
  JOURNAL   Sci Rep 11 (1), 8856 (2021)
   PUBMED   33893375
  REMARK    GeneRIF: Aggressive and recurrent ovarian cancers upregulate
            ephrinA5, a non-canonical effector of EphA2 signaling duality.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5372)
  AUTHORS   Xie,J., Xing,S., Shen,B.Y., Chen,H.T., Sun,B., Wang,Z.T., Wang,J.W.
            and Lu,X.X.
  TITLE     PIWIL1 interacting RNA piR-017061 inhibits pancreatic cancer growth
            via regulating EFNA5
  JOURNAL   Hum Cell 34 (2), 550-563 (2021)
   PUBMED   33389678
  REMARK    GeneRIF: PIWIL1 interacting RNA piR-017061 inhibits pancreatic
            cancer growth via regulating EFNA5.
REFERENCE   4  (bases 1 to 5372)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 5372)
  AUTHORS   Ricci,B., Millner,T.O., Pomella,N., Zhang,X., Guglielmi,L.,
            Badodi,S., Ceric,D., Gemma,C., Cognolato,E., Zhang,Y., Brandner,S.,
            Barnes,M.R. and Marino,S.
  TITLE     Polycomb-mediated repression of EphrinA5 promotes growth and
            invasion of glioblastoma
  JOURNAL   Oncogene 39 (12), 2523-2538 (2020)
   PUBMED   31988455
  REMARK    GeneRIF: Polycomb-mediated repression of EphrinA5 promotes growth
            and invasion of glioblastoma.
REFERENCE   6  (bases 1 to 5372)
  AUTHORS   Kozlosky,C.J., VandenBos,T., Park,L., Cerretti,D.P. and
            Carpenter,M.K.
  TITLE     LERK-7: a ligand of the Eph-related kinases is developmentally
            regulated in the brain
  JOURNAL   Cytokine 9 (8), 540-549 (1997)
   PUBMED   9245480
REFERENCE   7  (bases 1 to 5372)
  AUTHORS   Lackmann,M., Mann,R.J., Kravets,L., Smith,F.M., Bucci,T.A.,
            Maxwell,K.F., Howlett,G.J., Olsson,J.E., Vanden Bos,T.,
            Cerretti,D.P. and Boyd,A.W.
  TITLE     Ligand for EPH-related kinase (LERK) 7 is the preferred high
            affinity ligand for the HEK receptor
  JOURNAL   J Biol Chem 272 (26), 16521-16530 (1997)
   PUBMED   9195962
REFERENCE   8  (bases 1 to 5372)
  AUTHORS   Cerretti,D.P., Copeland,N.G., Gilbert,D.J., Jenkins,N.A.,
            Kuefer,M.U., Valentine,V., Shapiro,D.N., Cui,X. and Morris,S.W.
  TITLE     The gene encoding LERK-7 (EPLG7, Epl7), a ligand for the
            Eph-related receptor tyrosine kinases, maps to human chromosome 5
            at band q21 and to mouse chromosome 17
  JOURNAL   Genomics 35 (2), 376-379 (1996)
   PUBMED   8661153
REFERENCE   9  (bases 1 to 5372)
  AUTHORS   Gale,N.W., Holland,S.J., Valenzuela,D.M., Flenniken,A., Pan,L.,
            Ryan,T.E., Henkemeyer,M., Strebhardt,K., Hirai,H., Wilkinson,D.G.,
            Pawson,T., Davis,S. and Yancopoulos,G.D.
  TITLE     Eph receptors and ligands comprise two major specificity subclasses
            and are reciprocally compartmentalized during embryogenesis
  JOURNAL   Neuron 17 (1), 9-19 (1996)
   PUBMED   8755474
REFERENCE   10 (bases 1 to 5372)
  AUTHORS   Winslow,J.W., Moran,P., Valverde,J., Shih,A., Yuan,J.Q., Wong,S.C.,
            Tsai,S.P., Goddard,A., Henzel,W.J., Hefti,F. et al.
  TITLE     Cloning of AL-1, a ligand for an Eph-related tyrosine kinase
            receptor involved in axon bundle formation
  JOURNAL   Neuron 14 (5), 973-981 (1995)
   PUBMED   7748564
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC024587.5 and AC008952.7.
            
            On Nov 22, 2018 this sequence version replaced NM_001962.2.
            
            Summary: Ephrin-A5, a member of the ephrin gene family, prevents
            axon bundling in cocultures of cortical neurons with astrocytes, a
            model of late stage nervous system development and differentiation.
            The EPH and EPH-related receptors comprise the largest subfamily of
            receptor protein-tyrosine kinases and have been implicated in
            mediating developmental events, particularly in the nervous system.
            EPH receptors typically have a single kinase domain and an
            extracellular region containing a Cys-rich domain and 2 fibronectin
            type III repeats. The ephrin ligands and receptors have been named
            by the Eph Nomenclature Committee (1997). Based on their structures
            and sequence relationships, ephrins are divided into the ephrin-A
            (EFNA) class, which are anchored to the membrane by a
            glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB)
            class, which are transmembrane proteins. The Eph family of
            receptors are similarly divided into 2 groups based on the
            similarity of their extracellular domain sequences and their
            affinities for binding ephrin-A and ephrin-B ligands. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U26403.1, BQ277910.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000333274.11/ ENSP00000328777.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-449               AC024587.5         100803-101251
            450-742             AC008952.7         8821-9113
            743-808             AC008952.7         48559-48624
            809-889             AC008952.7         49015-49095
            890-5372            AC008952.7         54954-59436
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q21.3"
     gene            1..5372
                     /gene="EFNA5"
                     /gene_synonym="AF1; EFL5; EPLG7; GLC1M; LERK7; RAGS"
                     /note="ephrin A5"
                     /db_xref="GeneID:1946"
                     /db_xref="HGNC:HGNC:3225"
                     /db_xref="MIM:601535"
     exon            1..449
                     /gene="EFNA5"
                     /gene_synonym="AF1; EFL5; EPLG7; GLC1M; LERK7; RAGS"
                     /inference="alignment:Splign:2.1.0"
     CDS             325..1011
                     /gene="EFNA5"
                     /gene_synonym="AF1; EFL5; EPLG7; GLC1M; LERK7; RAGS"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; eph-related receptor tyrosine kinase ligand 7;
                     AL-1; LERK-7"
                     /codon_start=1
                     /product="ephrin-A5 isoform 1 precursor"
                     /protein_id="NP_001953.1"
                     /db_xref="CCDS:CCDS4097.1"
                     /db_xref="GeneID:1946"
                     /db_xref="HGNC:HGNC:3225"
                     /db_xref="MIM:601535"
                     /translation="MLHVEMLTLVFLVLWMCVFSQDPGSKAVADRYAVYWNSSNPRFQ
                     RGDYHIDVCINDYLDVFCPHYEDSVPEDKTERYVLYMVNFDGYSACDHTSKGFKRWEC
                     NRPHSPNGPLKFSEKFQLFTPFSLGFEFRPGREYFYISSAIPDNGRRSCLKLKVFVRP
                     TNSCMKTIGVHDRVFDVNDKVENSLEPADDTVHESAEPSRGENAAQTPRIPSRLLAIL
                     LFLLAMLLTL"
     exon            450..742
                     /gene="EFNA5"
                     /gene_synonym="AF1; EFL5; EPLG7; GLC1M; LERK7; RAGS"
                     /inference="alignment:Splign:2.1.0"
     exon            743..808
                     /gene="EFNA5"
                     /gene_synonym="AF1; EFL5; EPLG7; GLC1M; LERK7; RAGS"
                     /inference="alignment:Splign:2.1.0"
     exon            809..889
                     /gene="EFNA5"
                     /gene_synonym="AF1; EFL5; EPLG7; GLC1M; LERK7; RAGS"
                     /inference="alignment:Splign:2.1.0"
     exon            890..5372
                     /gene="EFNA5"
                     /gene_synonym="AF1; EFL5; EPLG7; GLC1M; LERK7; RAGS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2206..2211
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EFNA5"
                     /gene_synonym="AF1; EFL5; EPLG7; GLC1M; LERK7; RAGS"
                     /note="hexamer: AATAAA"
     polyA_site      2235
                     /gene="EFNA5"
                     /gene_synonym="AF1; EFL5; EPLG7; GLC1M; LERK7; RAGS"
                     /note="major polyA site"
     regulatory      4132..4137
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EFNA5"
                     /gene_synonym="AF1; EFL5; EPLG7; GLC1M; LERK7; RAGS"
                     /note="hexamer: AATAAA"
     polyA_site      4149
                     /gene="EFNA5"
                     /gene_synonym="AF1; EFL5; EPLG7; GLC1M; LERK7; RAGS"
     regulatory      5350..5355
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EFNA5"
                     /gene_synonym="AF1; EFL5; EPLG7; GLC1M; LERK7; RAGS"
                     /note="hexamer: ATTAAA"
     polyA_site      5372
                     /gene="EFNA5"
                     /gene_synonym="AF1; EFL5; EPLG7; GLC1M; LERK7; RAGS"
                     /note="major polyA site"
ORIGIN      
        1 acctcttccc ccgccctcgc cgccgccacc acacacacgc acgcttctct ccatcttgtg
       61 attccttttt cctcctgaac cctccagtgg gggtgcgagt ttgtctttat caccccccat
      121 cccaccgcct tcttttcttc tcgctctcct acccctcccc agcttggtgg gcgcctcttt
      181 cctttctcgc ccactttcat ttttatttat tcatatttat ttggcgcccg ctctctctct
      241 gtccctttgc ctgcctccct ccctccggat ccccgctctc tccccggagt ggcgcgtcgg
      301 gggctccgcc gctggccagg cgtgatgttg cacgtggaga tgttgacgct ggtgtttctg
      361 gtgctctgga tgtgtgtgtt cagccaggac ccgggctcca aggccgtcgc cgaccgctac
      421 gctgtctact ggaacagcag caaccccaga ttccagaggg gtgactacca tattgatgtc
      481 tgtatcaatg actacctgga tgttttctgc cctcactatg aggactccgt cccagaagat
      541 aagactgagc gctatgtcct ctacatggtg aactttgatg gctacagtgc ctgcgaccac
      601 acttccaaag ggttcaagag atgggaatgt aaccggcctc actctccaaa tggaccgctg
      661 aagttctctg aaaaattcca gctcttcact cccttttctc taggatttga attcaggcca
      721 ggccgagaat atttctacat ctcctctgca atcccagata atggaagaag gtcctgtcta
      781 aagctcaaag tctttgtgag accaacaaat agctgtatga aaactatagg tgttcatgat
      841 cgtgttttcg atgttaacga caaagtagaa aattcattag aaccagcaga tgacaccgta
      901 catgagtcag ccgagccatc ccgcggcgag aacgcggcac aaacaccaag gatacccagc
      961 cgccttttgg caatcctact gttcctcctg gcgatgcttt tgacattata gcacagtctc
     1021 ctcccatcac ttgtcacaga aaacatcagg gtcttggaac accagagatc cacctaactg
     1081 ctcatcctaa gaagggactt gttattgggt tttggcagat gtcagatttt tgttttcttt
     1141 ctttcagcct gaattctaag caacaacttc aggttggggg cctaaacttg ttcctgcctc
     1201 cctcacccca ccccgcccca cccccagccc tggcccttgg cttctctcac ccctcccaaa
     1261 ttaaatggac tccagatgaa aatgccaaat tgtcatagtg acaccagtgg ttcgtcagct
     1321 cctgtgcatt ctcctctaag aactcacctc cgttagcgca ctgtgtcagc gggctatgga
     1381 caaggaagaa tagtggcaga tgcagccagc gctggctagg gctgggaggg ttttgctctc
     1441 ctatgcaata tttatgcctt ctcattcaga actgtaagat gatcgcgcag ggcatcatgt
     1501 caccatgtca ggtccggagg ggaggtatta agaatagata cgatattaca ccatttccta
     1561 taggagtatg taaatgaaca ggcttctaaa aggttgagac actggttttt ttttttaata
     1621 tgactgtctt aaagcattct tgacagcaaa acttgtgctc tctaaaagaa gccttttttt
     1681 tttttctagg aggcaggttg ggtgtggaat gctaatacag agcaggtgtg aaaacagaga
     1741 aaactacagg tttgctgggg gtgtgtatgt gtgagtgcct ctaatttttt tggtgactgg
     1801 gcagtgcaca ccagatattt tttctttgaa tacagatcac catggtgcta caactttttt
     1861 tttttttttt tttttttttt tttttttttt taagaaactc aaagaggcat ttttatgaat
     1921 aaagtgacct tccccaaggc tgacaagcca gggttgatga gtgcatagtg gaatagcttt
     1981 ggatactcct ctgggggatg acatgtacca aggagaggac cgcagtggcc agaggagaca
     2041 tgatttggct ttgctggagc gccagtgtgc tgtggccttt ccccgcctcc caccctagta
     2101 cccacgtttt gctccacact ccttgaccgc aggggctcgg acacaaaccc ctgtcaccag
     2161 gagagtcagt cagcactact tgggagggct aaagggaaat ttggaaataa aattccaaag
     2221 tttggagtaa aaaaattcaa gtgttgattt tatattcttt ccctttctga cacagcctaa
     2281 agcgtagggg gaacatgtgt ttatctgtgg gagataaaca agatggagtc ccaaagactt
     2341 taacaaaata tttttttaaa aatccactag aatagaaaat acattattta gatatacttt
     2401 atgctgagag tgagtatata tgcttgtcct atttaaactt gtgagaaaaa gtggtatccc
     2461 ttgatacatt tagaaatatg ggggctatct tgtttcattg tgggggtggg gcagaaggag
     2521 aataaatgca ggatgaccct gttgaaggaa tcttagcatg gccaacaggg gacgtttcca
     2581 gtcgattacc aggaaatgca agccttgggg tttctactgg tggtggggct gtcatgaact
     2641 ttaaaatcca aagcctagac aaggaaaagt gttagaccaa ttgaaaagca atccagccct
     2701 tttttttttt ttttttttgg ctttgcacga catgtcaaca gaaaccatgc ctttcaatat
     2761 aagaaataaa tgtgatgatc atgtaaaatg tgaaaaattg aaagcattcc agcaaaataa
     2821 gaatttttta tatatttgtt ttttaagatg tatatgttaa aaaaagagaa ggtcgcatta
     2881 tggacagact tcgtgaatgg gaatttgctt agaattgtga gtagttctga attagaaaag
     2941 tatgtgaagg aaaggcagct gtaaacgtat tgtgccctgg agagttgtac acatgttgaa
     3001 atgtaatctg ggcttacctg atccatttgg agtggatgtc actgccgagt ctgttctcac
     3061 atggaaccat gtgtgtgggg ttgccagcct cacagataca atcaatccta ttcccctctg
     3121 acataaggaa ctcctctgga gtggcagagt cttatcacag aaggcagcca ccatttcacc
     3181 aaaacaaaag ttcacggcat tcaattcctt tttcctttag ctatttatat atgcagtact
     3241 ctcagtcata tgcagaaata cttttttttt tttaattaat agttacaggc ttgttggtcc
     3301 agtgggattt gggtaggggg agaaagatac cttctaaaat ggatcaatag aaccaaaata
     3361 atacagcatg ttctataacc acaaggaaat caaatgatcc tgtcatgatt ccagttagtc
     3421 ataactatgt tagcagtgct aaatgcattt tagaaatggt gacttctgtg gttttcctag
     3481 catttgtctc taacaaatgg tgaaataatt actcatggcc ctctctgcca ttgtctttca
     3541 ttttttcaca gtgaaattag acccctttac ttcaccattc tgccactgca aattaagtat
     3601 aaagaaaata gcaagagtgt ccacaccagt agacagtaag cttctctacc tgtaagtgat
     3661 gaaatcatag ctaatgcact tgccatggag ttttcaagat gattggtgtc agacagtttt
     3721 cactttgttt aaaaagtgtt ggtggccttt tgtggtggtg ttacaatcct ctgggggctt
     3781 aggaggatgt tgatgcaact tttagaagct tttaatttca aaaacaactc aaaaatctga
     3841 aggacagtca tagctgccac tcagccccag ttagtcaaac cccagtgacc tttgcccctg
     3901 gttgccaagg gctttgcaac atcaagcagg gaaataagga tctgtctgtt tagtggatac
     3961 cgtgtatcct ttaatagacc aggtaacagt tcgtgttagt ttagactatt gttttgtact
     4021 gtactttctt gggtggcaga ggaaagaaaa gtaaaacatt aaaaaaaaaa aaaaaactgc
     4081 gttctttaaa ttctgtatta ttagcaacct ctgttgtaca tagtgtttga taataaagta
     4141 ttaatttgat tcttatgtct tttgtaagtg agaacaatag actttcagga tacaaaacat
     4201 gcattgaggc tttgaaacat ccaatgtgta ccatggctga aaaaaagagt cacaagtggc
     4261 tgagacactg ctccatacag gactcatgtg tggtcattgc ccacccaatc ttatgactcc
     4321 atcatcttgg gacctggaac aacatgactt ttttgatcga tattttgtcc tcggtgtttt
     4381 caaggtctga tgttggagcc ctgtggccca ccctcccttc tccaccagca tgttggtttg
     4441 aaaaggatag ggaatttaga aacagttctg tactttggtt tggtttggtt ttctgtttgt
     4501 gattgttttc atggactgtt ttattttttc ccaggaagag tctttatcaa tatcatgtgc
     4561 agctcactca tggaaatggt tgcaaaccaa tcagtgtagg aagcttaaat ggggctgttt
     4621 ccctctctgt gtcgttgtga aaggaagagt cacacagtac ctgtggattt tcagggactc
     4681 tgtttttctc cggtgcctta gcaacggcag cagccatttg tattattttc aaataattag
     4741 aaaaacagtt ttcaactcct cctttccatt cattgctctc agagttgtgg tcactgactt
     4801 ttcttttgaa aaccgcctcc accaacaccc ccgtttgcct acaccacccc ccttttactt
     4861 agtatgttta ttttttgtgt gtctcttgcc ttcctcccac gttttatttc ccctcagagc
     4921 tgtgaatggg caggtctgtc tctggtttgg catcactgag tttttcccat gcattggccc
     4981 cagggctgct aggatgtgag acaaatctcc ctacaatggg cttgctccca ttgtctgtac
     5041 agtttaatag atgctggcat gtcggaggtt acccatgagt caaaatccgc tctccatgct
     5101 tactcttgac accccattga agccactcat tgtgtgtgcg tctgggtgtg aagtccagct
     5161 ccgtgtggtc ctgtgcttgt actgccctgc tttgcagttc ctttgcactt actcatcgag
     5221 tgctgttttg aaatgctgac attatataaa cgtaaaagaa aatgtaaaaa aaaaaaaccc
     5281 acacacaaac aaacccatac gatctgtatt tgtatataca cgtgtccgta caagtataac
     5341 taaataaaaa ttaaagattt tcatcatttt aa
//
