LOCUS       NM_025184               3257 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens EF-hand domain containing 2 (EFHC2), mRNA.
ACCESSION   NM_025184 NM_024822
VERSION     NM_025184.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Gui,M., Croft,J.T., Zabeo,D., Acharya,V., Kollman,J.M.,
            Burgoyne,T., Hoog,J.L. and Brown,A.
  TITLE     SPACA9 is a lumenal protein of human ciliary singlet and doublet
            microtubules
  JOURNAL   Proc Natl Acad Sci U S A 119 (41), e2207605119 (2022)
   PUBMED   36191189
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 3257)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3257)
  AUTHORS   Chen,S., Fragoza,R., Klei,L., Liu,Y., Wang,J., Roeder,K., Devlin,B.
            and Yu,H.
  TITLE     An interactome perturbation framework prioritizes damaging missense
            mutations for developmental disorders
  JOURNAL   Nat Genet 50 (7), 1032-1040 (2018)
   PUBMED   29892012
REFERENCE   5  (bases 1 to 3257)
  AUTHORS   Jia,B., Huang,L., Chen,Y., Liu,S., Chen,C., Xiong,K., Song,L.,
            Zhou,Y., Yang,X. and Zhong,M.
  TITLE     A novel contiguous deletion involving NDP, MAOB and EFHC2 gene in a
            patient with familial Norrie disease: bilateral blindness and
            leucocoria without other deficits
  JOURNAL   J Genet 96 (6), 1015-1020 (2017)
   PUBMED   29321361
  REMARK    GeneRIF: Our observations provide new information on the
            genotype-phenotype relations of MAOA/B and EFHC2 genes involved in
            the contiguous deletions of Norrie disease.Based on the case of our
            observation, contiguous deletion with only one of the MAO genes
            (MAOB) may not cause psychomotor disability, and deletion of
            EFHC2may not contribute to epilepsy.
REFERENCE   6  (bases 1 to 3257)
  AUTHORS   Zinn,A.R., Kushner,H. and Ross,J.L.
  TITLE     EFHC2 SNP rs7055196 is not associated with fear recognition in 45,X
            Turner syndrome
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 147B (4), 507-509 (2008)
   PUBMED   17948898
  REMARK    GeneRIF: no evidence of an association between rs7055196 genotype
            and fear recognition
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 3257)
  AUTHORS   de Brouwer,A.P., Yntema,H.G., Kleefstra,T., Lugtenberg,D.,
            Oudakker,A.R., de Vries,B.B., van Bokhoven,H., Van Esch,H.,
            Frints,S.G., Froyen,G., Fryns,J.P., Raynaud,M., Moizard,M.P.,
            Ronce,N., Bensalem,A., Moraine,C., Poirier,K., Castelnau,L.,
            Saillour,Y., Bienvenu,T., Beldjord,C., des Portes,V., Chelly,J.,
            Turner,G., Fullston,T., Gecz,J., Kuss,A.W., Tzschach,A.,
            Jensen,L.R., Lenzner,S., Kalscheuer,V.M., Ropers,H.H. and
            Hamel,B.C.
  TITLE     Mutation frequencies of X-linked mental retardation genes in
            families from the EuroMRX consortium
  JOURNAL   Hum Mutat 28 (2), 207-208 (2007)
   PUBMED   17221867
REFERENCE   8  (bases 1 to 3257)
  AUTHORS   Weiss,L.A., Purcell,S., Waggoner,S., Lawrence,K., Spektor,D.,
            Daly,M.J., Sklar,P. and Skuse,D.
  TITLE     Identification of EFHC2 as a quantitative trait locus for fear
            recognition in Turner syndrome
  JOURNAL   Hum Mol Genet 16 (1), 107-113 (2007)
   PUBMED   17164267
  REMARK    GeneRIF: EFHC2 shows genealogy and extended LD consistent with
            directional selection. This novel QTL may influence social
            cognition in the general population and in autism.
REFERENCE   9  (bases 1 to 3257)
  AUTHORS   Gu,W., Sander,T., Heils,A., Lenzen,K.P. and Steinlein,O.K.
  TITLE     A new EF-hand containing gene EFHC2 on Xp11.4: tentative evidence
            for association with juvenile myoclonic epilepsy
  JOURNAL   Epilepsy Res 66 (1-3), 91-98 (2005)
   PUBMED   16112844
  REMARK    GeneRIF: An association with the gonosomal gene EFHC2 would be in
            accordance with the observed preponderance of maternal inheritance
            in juvenile myoclonic epilepsy maternal inheritance of juvenile
            myoclonic epilepsy.
REFERENCE   10 (bases 1 to 3257)
  AUTHORS   Ross,M.T., Grafham,D.V., Coffey,A.J., Scherer,S., McLay,K.,
            Muzny,D., Platzer,M., Howell,G.R., Burrows,C., Bird,C.P.,
            Frankish,A., Lovell,F.L., Howe,K.L., Ashurst,J.L., Fulton,R.S.,
            Sudbrak,R., Wen,G., Jones,M.C., Hurles,M.E., Andrews,T.D.,
            Scott,C.E., Searle,S., Ramser,J., Whittaker,A., Deadman,R.,
            Carter,N.P., Hunt,S.E., Chen,R., Cree,A., Gunaratne,P., Havlak,P.,
            Hodgson,A., Metzker,M.L., Richards,S., Scott,G., Steffen,D.,
            Sodergren,E., Wheeler,D.A., Worley,K.C., Ainscough,R.,
            Ambrose,K.D., Ansari-Lari,M.A., Aradhya,S., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Ballabio,A., Banerjee,R., Barker,G.E.,
            Barlow,K.F., Barrett,I.P., Bates,K.N., Beare,D.M., Beasley,H.,
            Beasley,O., Beck,A., Bethel,G., Blechschmidt,K., Brady,N.,
            Bray-Allen,S., Bridgeman,A.M., Brown,A.J., Brown,M.J., Bonnin,D.,
            Bruford,E.A., Buhay,C., Burch,P., Burford,D., Burgess,J.,
            Burrill,W., Burton,J., Bye,J.M., Carder,C., Carrel,L., Chako,J.,
            Chapman,J.C., Chavez,D., Chen,E., Chen,G., Chen,Y., Chen,Z.,
            Chinault,C., Ciccodicola,A., Clark,S.Y., Clarke,G., Clee,C.M.,
            Clegg,S., Clerc-Blankenburg,K., Clifford,K., Cobley,V., Cole,C.G.,
            Conquer,J.S., Corby,N., Connor,R.E., David,R., Davies,J., Davis,C.,
            Davis,J., Delgado,O., Deshazo,D., Dhami,P., Ding,Y., Dinh,H.,
            Dodsworth,S., Draper,H., Dugan-Rocha,S., Dunham,A., Dunn,M.,
            Durbin,K.J., Dutta,I., Eades,T., Ellwood,M., Emery-Cohen,A.,
            Errington,H., Evans,K.L., Faulkner,L., Francis,F., Frankland,J.,
            Fraser,A.E., Galgoczy,P., Gilbert,J., Gill,R., Glockner,G.,
            Gregory,S.G., Gribble,S., Griffiths,C., Grocock,R., Gu,Y.,
            Gwilliam,R., Hamilton,C., Hart,E.A., Hawes,A., Heath,P.D.,
            Heitmann,K., Hennig,S., Hernandez,J., Hinzmann,B., Ho,S., Hoffs,M.,
            Howden,P.J., Huckle,E.J., Hume,J., Hunt,P.J., Hunt,A.R.,
            Isherwood,J., Jacob,L., Johnson,D., Jones,S., de Jong,P.J.,
            Joseph,S.S., Keenan,S., Kelly,S., Kershaw,J.K., Khan,Z.,
            Kioschis,P., Klages,S., Knights,A.J., Kosiura,A., Kovar-Smith,C.,
            Laird,G.K., Langford,C., Lawlor,S., Leversha,M., Lewis,L., Liu,W.,
            Lloyd,C., Lloyd,D.M., Loulseged,H., Loveland,J.E., Lovell,J.D.,
            Lozado,R., Lu,J., Lyne,R., Ma,J., Maheshwari,M., Matthews,L.H.,
            McDowall,J., McLaren,S., McMurray,A., Meidl,P., Meitinger,T.,
            Milne,S., Miner,G., Mistry,S.L., Morgan,M., Morris,S., Muller,I.,
            Mullikin,J.C., Nguyen,N., Nordsiek,G., Nyakatura,G., O'Dell,C.N.,
            Okwuonu,G., Palmer,S., Pandian,R., Parker,D., Parrish,J.,
            Pasternak,S., Patel,D., Pearce,A.V., Pearson,D.M., Pelan,S.E.,
            Perez,L., Porter,K.M., Ramsey,Y., Reichwald,K., Rhodes,S.,
            Ridler,K.A., Schlessinger,D., Schueler,M.G., Sehra,H.K.,
            Shaw-Smith,C., Shen,H., Sheridan,E.M., Shownkeen,R., Skuce,C.D.,
            Smith,M.L., Sotheran,E.C., Steingruber,H.E., Steward,C.A.,
            Storey,R., Swann,R.M., Swarbreck,D., Tabor,P.E., Taudien,S.,
            Taylor,T., Teague,B., Thomas,K., Thorpe,A., Timms,K., Tracey,A.,
            Trevanion,S., Tromans,A.C., d'Urso,M., Verduzco,D., Villasana,D.,
            Waldron,L., Wall,M., Wang,Q., Warren,J., Warry,G.L., Wei,X.,
            West,A., Whitehead,S.L., Whiteley,M.N., Wilkinson,J.E.,
            Willey,D.L., Williams,G., Williams,L., Williamson,A.,
            Williamson,H., Wilming,L., Woodmansey,R.L., Wray,P.W., Yen,J.,
            Zhang,J., Zhou,J., Zoghbi,H., Zorilla,S., Buck,D., Reinhardt,R.,
            Poustka,A., Rosenthal,A., Lehrach,H., Meindl,A., Minx,P.J.,
            Hillier,L.W., Willard,H.F., Wilson,R.K., Waterston,R.H., Rice,C.M.,
            Vaudin,M., Coulson,A., Nelson,D.L., Weinstock,G., Sulston,J.E.,
            Durbin,R., Hubbard,T., Gibbs,R.A., Beck,S., Rogers,J. and
            Bentley,D.R.
  TITLE     The DNA sequence of the human X chromosome
  JOURNAL   Nature 434 (7031), 325-337 (2005)
   PUBMED   15772651
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC018719.4, AL133344.28 and AL359744.17.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_025184.3.
            
            Summary: This gene encodes a protein which contains three DM10
            domains and three calcium-binding EF-hand motifs. A related protein
            is encoded by a gene on chromosome 6. It has been suggested that
            both proteins are involved in the development of epilepsy (PMID:
            15258581, 16112844) and that this gene may be associated with fear
            recognition in individuals with Turner syndrome. [provided by
            RefSeq, Aug 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031039.1, AK292967.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000420999.2/ ENSP00000404232.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-126               AC018719.4         48657-48782         c
            127-315             AL133344.28        150195-150383       c
            316-466             AL133344.28        110313-110463       c
            467-690             AL133344.28        98702-98925         c
            691-942             AL133344.28        87821-88072         c
            943-1056            AL133344.28        86430-86543         c
            1057-1195           AL133344.28        85899-86037         c
            1196-1364           AL133344.28        79748-79916         c
            1365-1507           AL133344.28        72932-73074         c
            1508-1704           AL133344.28        70108-70304         c
            1705-1835           AL133344.28        67276-67406         c
            1836-2033           AL133344.28        15994-16191         c
            2034-2126           AL133344.28        13919-14011         c
            2127-2232           AL133344.28        1549-1654           c
            2233-3257           AL359744.17        108249-109273       c
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp11.3"
     gene            1..3257
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /note="EF-hand domain containing 2"
                     /db_xref="GeneID:80258"
                     /db_xref="HGNC:HGNC:26233"
                     /db_xref="MIM:300817"
     exon            1..126
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..2334
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /note="EF-hand domain (C-terminal) containing 2"
                     /codon_start=1
                     /product="EF-hand domain-containing family member C2"
                     /protein_id="NP_079460.2"
                     /db_xref="CCDS:CCDS55405.1"
                     /db_xref="GeneID:80258"
                     /db_xref="HGNC:HGNC:26233"
                     /db_xref="MIM:300817"
                     /translation="MALPLLPGNSFNRNVGKEKFHKSQHWGFCNNVMMLVSDEKPGIG
                     GEPLLGQKIKPKCSIYPKGDGSDVPSWVAFDKQVLSFDAYLEEEVLDKSQTNYRIRYY
                     KIYFYPEDDTIQVNEPEVKNSGLLQGTSIRRHRITLPPPDEDQFYTVYHFNVGTEVVF
                     YGRTFKIYDCDAFTRNFLRKIGVKVNPPVQCPEDPYMKIRREVVEHVEPLRPYESLDT
                     LKQFLQYHGKILCFFCLWDDSVSMFGDRRELILHYFLCDDTIEIKELLPHSSGRDALK
                     MFLRRSKLPKNCPPRVYQPGQITDRAVLNSYGDFIKNQADGYLFDRYKLGKVDQEFYK
                     DSDLSLGVTINVWGRKVLLYDCDEFTKSYYKSKYGIENFTSVSCKPPSPPPKIERKFP
                     PYNGFGSEEDSLRNCIDLKPTPHRRNFKKFMEKDSYGSKSNILRFFAKLVTDKCVDLD
                     RMFVISYYLGDDTISVFEPIERNSGIAGGMFLKRSRVKKPGQEVFKSELSEYIKAEEL
                     YIGVTVNVNGYLFRLLNADEYTLNYMEQNTDKYPFSNLKLALQKLKQEEGKSRELKQV
                     FKAADSKHTNMVDYNTFRDILMSLTVGNLAEQEFVTIARHYRVPEGTCSDMDFLIALA
                     HEKFKKNMFENFDTFIYSCVYEDREKKNVLPTKDIKRLCKSSRLPLSDDLLESLLSRF
                     EDSEKQIDYKSFFSALNWRKNPVPELQPASYLKERCEDVWLGMPSPIPAKYIDYWTFL
                     KDAFGLEEE"
     exon            127..315
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     exon            316..466
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     exon            467..690
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     exon            691..942
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1056
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1195
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1364
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1507
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1704
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1835
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..2033
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     exon            2034..2126
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     exon            2127..2232
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..3257
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2439..2444
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /note="hexamer: ATTAAA"
     polyA_site      2464
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
     regulatory      2719..2724
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /note="hexamer: ATTAAA"
     polyA_site      2739
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /note="major polyA site"
     regulatory      3227..3232
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
                     /note="hexamer: ATTAAA"
     polyA_site      3257
                     /gene="EFHC2"
                     /gene_synonym="dJ1158H2.1; MRX74"
ORIGIN      
        1 gggggccaaa caacacggcc gggaggcgcc gctgcctgcc gggccctctc ttctgggacc
       61 ctggattttc ggacactgag cgccatggcc ctgcctctgc tgccgggcaa cagcttcaac
      121 cgcaacgtgg gaaaggagaa gtttcacaaa tcccaacatt ggggcttttg caacaatgtt
      181 atgatgttgg tgagtgatga aaagcctgga ataggtggag aaccacttct ggggcaaaag
      241 ataaagccta aatgtagcat atatcctaaa ggagatggaa gtgatgtacc atcatgggta
      301 gcctttgata aacaggtatt atcttttgat gcctatttgg aagaggaagt acttgataaa
      361 agccaaacca actacagaat aagatactat aaaatctact tctaccctga agatgacaca
      421 attcaagtaa atgaaccaga ggtgaaaaat agtggattac ttcaagggac ttctatccgg
      481 cgtcatcgga ttactcttcc gcctcctgat gaggatcagt tttatactgt gtatcatttt
      541 aatgtcggca cagaggttgt cttctatggc cggacattca agatttatga ctgtgatgca
      601 ttcacaagaa actttttgag gaaaataggg gtcaaagtga atcccccagt gcaatgtcca
      661 gaagatcctt acatgaagat tcggagagag gttgtagaac acgtagagcc cttacgtccc
      721 tacgaatccc tcgacaccct gaaacagttc ctccagtatc atggcaagat tttgtgtttc
      781 ttctgcctgt gggatgactc agtctcaatg tttggagacc gtagagaact catcctgcat
      841 tacttcttgt gtgatgatac tattgaaatc aaagaattgc ttccacacag ctcaggccga
      901 gatgctctaa aaatgttcct ccggaggagt aagctaccca agaattgccc acctagagtc
      961 tatcaaccag gccagataac agatcgagca gttctcaatt catatggtga ctttataaag
     1021 aaccaagcgg atggctacct gttcgataga tataagctag gaaaagtaga ccaagagttt
     1081 tacaaagata gtgacctgtc cctaggagtc accatcaatg tgtggggaag aaaagtgctc
     1141 ctttatgact gtgatgaatt tacgaagtct tattataagt ctaaatatgg aattgagaac
     1201 tttacctcag tttcatgcaa gcctccttct cctcctccaa aaatagaaag gaaatttcca
     1261 ccttacaacg gttttggttc tgaagaggat tctctccgta actgcataga cctcaagccc
     1321 acacctcatc ggaggaactt caagaagttt atggaaaaag acagctatgg ctccaaaagc
     1381 aatatactcc gtttttttgc aaaactagtc acagacaaat gtgttgactt ggacaggatg
     1441 tttgttattt catattatct cggtgatgac accatttcag tgtttgaacc tatagagagg
     1501 aattcaggaa ttgctggtgg gatgttcttg aaaagaagtc gcgttaagaa gcctggacaa
     1561 gaagtcttta aaagtgaact atctgaatat atcaaggccg aggagctgta cattggagtc
     1621 acggtgaatg tgaatggtta cctatttcgt ttgctcaatg ctgatgagta taccttaaac
     1681 tacatggagc agaatacaga taagtatcct ttcagtaacc tcaaacttgc cctacaaaag
     1741 ctgaagcaag aagaaggaaa atccagagag ctcaagcagg tatttaaagc tgctgactct
     1801 aagcacacaa atatggtgga ttataataca ttcagagaca tattgatgtc tttgactgtt
     1861 ggaaaccttg cagagcaaga atttgtaacc attgcacgtc actaccgtgt gcctgagggc
     1921 acatgttcag atatggattt cttaatcgca ctggcccacg aaaagttcaa gaaaaatatg
     1981 tttgagaatt tcgacacttt catttattcc tgtgtgtatg aagatcgaga aaaaaaaaat
     2041 gtattaccca ccaaagacat taaaaggctg tgcaaatcct ccagattacc tttgagtgat
     2101 gatcttctag aatccttatt gtcaaggttt gaagacagtg aaaaacaaat agattataag
     2161 tcatttttct ctgccctgaa ctggagaaag aatccagtgc ctgaattgca accagcatca
     2221 taccttaaag agagatgtga agatgtttgg cttggtatgc catcacctat tcctgcgaaa
     2281 tacattgact actggacctt tttgaaggac gcgtttggct tagaggagga ataaccatgc
     2341 cagttttggt caattctcta tgatttactt ctctcatttt gccacattta ctttagtaga
     2401 tataatttca ttaaaaacaa aaaagaaaca aggtttatat taaatggaaa tccataaacc
     2461 acaattaatc ctattttgtc atgttagttt tacagaacat catgttacat tgccgacgta
     2521 ctgttactat ttaaaatgcc atcttaaaac atgttgaata tttttattat atgtatggtt
     2581 tattaatacc aaaaaatctt ctgataattt ttgatccaac ataatttgat tcattactat
     2641 atgaacagaa aatgactttt gaaggttata catgacaata caaaatcaaa ttatatcaca
     2701 gcaataagtt gtgattctat taaacatttt tgaaaccttt ttgacacgtt tattacttac
     2761 ataattaagt tttgtagcaa cccacctcat caaaataatt cttaaacctc tctagagtaa
     2821 atatatttat cttttgaact tagtaaagct atgggaagaa aaagaacctc caacacacac
     2881 acacacacac acacacacgc acgtgcacac acactggaaa catatgcagt atttatattt
     2941 gtaatgccct tttttctatc taaagtcagc ttgttagctt aggaatatat gcatgtgtgt
     3001 ataattatag tttgaccaaa actttattat gtctaaaata tttacaaatg tgaaagctga
     3061 agattttgag atttttgttt tttttttggt ttgttttaca cacacatccc acgcgcccca
     3121 ccctagaggt ttaaatattc agaaactaca acatttttcc cctgcatttt tctagttcta
     3181 tttttatttt tcttcagcag aactcttgtc atgtagtgta catttgatta aaacccatag
     3241 ccatgtacct ctgggaa
//
