LOCUS       NM_018100               6849 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens EF-hand domain containing 1 (EFHC1), transcript
            variant A, mRNA.
ACCESSION   NM_018100
VERSION     NM_018100.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Aslan-Kara,K., Dundar-Yenilmez,E., Ates,E., Alparslan,M.M.,
            Pekoz,T., Bozdemir,H. and Tuli,A.
  TITLE     EFHC1 gene mutation profile of Turkish JME patients and its
            association with disease risk
  JOURNAL   Seizure 114, 79-83 (2024)
   PUBMED   38088014
  REMARK    GeneRIF: EFHC1 gene mutation profile of Turkish JME patients and
            its association with disease risk.
REFERENCE   2  (bases 1 to 6849)
  AUTHORS   Gui,M., Croft,J.T., Zabeo,D., Acharya,V., Kollman,J.M.,
            Burgoyne,T., Hoog,J.L. and Brown,A.
  TITLE     SPACA9 is a lumenal protein of human ciliary singlet and doublet
            microtubules
  JOURNAL   Proc Natl Acad Sci U S A 119 (41), e2207605119 (2022)
   PUBMED   36191189
REFERENCE   3  (bases 1 to 6849)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 6849)
  AUTHORS   Saleem,T., Mustafa,A., Sheikh,N., Mukhtar,M., Irfan,M. and
            Suqaina,S.K.
  TITLE     Mutational Analysis of Myoclonin1 Gene in Pakistani Juvenile
            Myoclonic Epilepsy Patients
  JOURNAL   Biomed Res Int 2021, 7509825 (2021)
   PUBMED   33969125
  REMARK    GeneRIF: Mutational Analysis of Myoclonin1 Gene in Pakistani
            Juvenile Myoclonic Epilepsy Patients.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6849)
  AUTHORS   Gonsales,M.C., Ribeiro,P.A.O., Betting,L.E., Alvim,M.K.M.,
            Guerreiro,C.M., Yasuda,C.L., Gitai,D.L.G., Cendes,F. and
            Lopes-Cendes,I.
  TITLE     Revisiting the clinical impact of variants in EFHC1 in patients
            with different phenotypes of genetic generalized epilepsy
  JOURNAL   Epilepsy Behav 112, 107469 (2020)
   PUBMED   33181902
  REMARK    GeneRIF: Revisiting the clinical impact of variants in EFHC1 in
            patients with different phenotypes of genetic generalized epilepsy.
REFERENCE   6  (bases 1 to 6849)
  AUTHORS   Norberg,A., Forsgren,L., Holmberg,D. and Holmberg,M.
  TITLE     Exclusion of the juvenile myoclonic epilepsy gene EFHC1 as the
            cause of migraine on chromosome 6, but association to two rare
            polymorphisms in MEP1A and RHAG
  JOURNAL   Neurosci Lett 396 (2), 137-142 (2006)
   PUBMED   16378686
  REMARK    GeneRIF: The combination of these polymorphisms could not be found
            in any control individuals, suggesting that they might be involved
            in genetic predisposition to migraine in this family.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 6849)
  AUTHORS   Suzuki,T., Delgado-Escueta,A.V., Aguan,K., Alonso,M.E., Shi,J.,
            Hara,Y., Nishida,M., Numata,T., Medina,M.T., Takeuchi,T.,
            Morita,R., Bai,D., Ganesh,S., Sugimoto,Y., Inazawa,J., Bailey,J.N.,
            Ochoa,A., Jara-Prado,A., Rasmussen,A., Ramos-Peek,J., Cordova,S.,
            Rubio-Donnadieu,F., Inoue,Y., Osawa,M., Kaneko,S., Oguni,H.,
            Mori,Y. and Yamakawa,K.
  TITLE     Mutations in EFHC1 cause juvenile myoclonic epilepsy
  JOURNAL   Nat Genet 36 (8), 842-849 (2004)
   PUBMED   15258581
  REMARK    GeneRIF: Mutation analyses identified five missense mutations in
            EFHC1 that cosegregated with epilepsy or EEG polyspike wave in
            affected members of six unrelated families with JME and did not
            occur in 382 control individuals
            GeneRIF: Observational study of genotype prevalence. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 6849)
  AUTHORS   Izzi,C., Barbon,A., Toliat,M.R., Heils,A., Becker,C., Nurnberg,P.,
            Sander,T. and Barlati,S.
  TITLE     Candidate gene analysis of the human metabotropic glutamate
            receptor type 4 (GRM4) in patients with juvenile myoclonic epilepsy
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 123B (1), 59-63 (2003)
   PUBMED   14582146
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 6849)
  AUTHORS   Sander,T., Bockenkamp,B., Hildmann,T., Blasczyk,R., Kretz,R.,
            Wienker,T.F., Volz,A., Schmitz,B., Beck-Mannagetta,G., Riess,O.,
            Epplen,J.T., Janz,D. and Ziegler,A.
  TITLE     Refined mapping of the epilepsy susceptibility locus EJM1 on
            chromosome 6
  JOURNAL   Neurology 49 (3), 842-847 (1997)
   PUBMED   9305351
REFERENCE   10 (bases 1 to 6849)
  AUTHORS   Liu,A.W., Delgado-Escueta,A.V., Gee,M.N., Serratosa,J.M.,
            Zhang,Q.W., Alonso,M.E., Medina,M.T., Cordova,S., Zhao,H.Z.,
            Spellman,J.M., Donnadieu,F.R., Peek,J.R., Treiman,L.J. and
            Sparkes,R.S.
  TITLE     Juvenile myoclonic epilepsy in chromosome 6p12-p11: locus
            heterogeneity and recombinations
  JOURNAL   Am J Med Genet 63 (3), 438-446 (1996)
   PUBMED   8737649
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK001328.1, BC020210.1,
            AL049611.24 and BQ270560.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_018100.3.
            
            Summary: This gene encodes an EF-hand-containing calcium binding
            protein. The encoded protein likely plays a role in calcium
            homeostasis. Mutations in this gene have been associated with
            susceptibility to juvenile myoclonic epilepsy and juvenile absence
            epilepsy. Alternatively spliced transcript variants have been
            described. [provided by RefSeq, Feb 2010].
            
            Transcript Variant: This variant (A) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.225459.1,
                                           SRR1660803.49357.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371068.11/ ENSP00000360107.4
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1263              AK001328.1         33-1295
            1264-1923           BC020210.1         1286-1945
            1924-2067           AK001328.1         1956-2099
            2068-5344           AL049611.24        16010-19286
            5345-5436           BQ270560.1         15-106              c
            5437-6849           AL049611.24        19379-20791
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p12.2"
     gene            1..6849
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /note="EF-hand domain containing 1"
                     /db_xref="GeneID:114327"
                     /db_xref="HGNC:HGNC:16406"
                     /db_xref="MIM:608815"
     exon            1..132
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..1992
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /note="isoform 1 is encoded by transcript variant A;
                     EF-hand domain-containing protein 1; myoclonin-1; EF-hand
                     domain (C-terminal) containing 1"
                     /codon_start=1
                     /product="EF-hand domain-containing protein 1 isoform 1"
                     /protein_id="NP_060570.2"
                     /db_xref="CCDS:CCDS4942.1"
                     /db_xref="GeneID:114327"
                     /db_xref="HGNC:HGNC:16406"
                     /db_xref="MIM:608815"
                     /translation="MVSNPVHGLPFLPGTSFKDSTKTAFHRSQTLSYRNGYAIVRRPT
                     VGIGGDRLQFNQLSQAELDELASKAPVLTYGQPKQAPPADFIPAHVAFDKKVLKFDAY
                     FQEDVPMSTEEQYRIRQVNIYYYLEDDSMSVIEPVVENSGILQGKLIKRQRLAKNDRG
                     DHYHWKDLNRGINITIYGKTFRVVDCDQFTQVFLESQGIELNPPEKMALDPYTELRKQ
                     PLRKYVTPSDFDQLKQFLTFDKQVLRFYAIWDDTDSMYGECRTYIIHYYLMDDTVEIR
                     EVHERNDGRDPFPLLMNRQRVPKVLVENAKNFPQCVLEISDQEVLEWYTAKDFIVGKS
                     LTILGRTFFIYDCDPFTRRYYKEKFGITDLPRIDVSKREPPPVKQELPPYNGFGLVED
                     SAQNCFALIPKAPKKDVIKMLVNDNKVLRYLAVLESPIPEDKDRRFVFSYFLATDMIS
                     IFEPPVRNSGIIGGKYLGRTKVVKPYSTVDNPVYYGPSDFFIGAVIEVFGHRFIILDT
                     DEYVLKYMESNAAQYSPEALASIQNHVRKREAPAPEAESKQTEKDPGVQELEALIDTI
                     QKQLKDHSCKDNIREAFQIYDKEASGYVDRDMFFKICESLNVPVDDSLVKELIRMCSH
                     GEGKINYYNFVRAFSN"
     exon            133..354
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /inference="alignment:Splign:2.1.0"
     exon            355..642
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /inference="alignment:Splign:2.1.0"
     exon            643..792
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /inference="alignment:Splign:2.1.0"
     exon            793..985
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1206
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1347
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1561
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1709
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..1920
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /inference="alignment:Splign:2.1.0"
     exon            1921..6849
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2045..2050
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /note="hexamer: AATACA"
     polyA_site      2072
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /note="major polyA site"
     regulatory      3783..3788
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /note="hexamer: AATAAA"
     polyA_site      3807
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
     regulatory      5403..5408
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /note="hexamer: AATAAA"
     polyA_site      5436
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
     regulatory      5982..5987
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /note="hexamer: AATAAA"
     polyA_site      6006
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
     regulatory      6815..6820
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
                     /note="hexamer: AATAAA"
     polyA_site      6849
                     /gene="EFHC1"
                     /gene_synonym="dJ304B14.2; EJM1; POC9; RIB72"
ORIGIN      
        1 actgcttgtc gcctgggcaa ccggagagga cgaagcagga cctaggtggc ggcggtggta
       61 ccggctgcaa tggtgtccaa tcccgtgcat ggcttgccct ttcttccggg cacgtccttt
      121 aaggactcta cgaaaacagc cttccacaga agtcagacgc tgagctacag gaacggctat
      181 gcaattgttc gacgtccaac agttgggata ggcggagacc ggctccagtt caaccagctg
      241 tcccaggctg agctggatga gttggccagt aaggcaccag tcttaactta tggccaacct
      301 aaacaagccc cacctgcgga ttttattcct gcgcatgtgg cctttgacaa aaaggtactg
      361 aaatttgatg cctatttcca agaagatgtt cctatgtcaa ctgaggaaca gtataggatc
      421 cgtcaggtga acatttacta ttatctagaa gatgacagca tgtctgtcat agagcctgtt
      481 gtagaaaatt ctggaatcct tcaaggcaag ttaataaaac gccagcggct agccaagaat
      541 gaccggggtg accattacca ttggaaagac ctaaatcgag gaataaacat cacaatttat
      601 ggcaaaactt tccgcgttgt tgactgtgac caattcacac aggtattttt agaaagccaa
      661 ggaattgagt taaatccacc agagaagatg gctcttgatc cttacactga actccgaaaa
      721 cagcctcttc gtaagtatgt caccccatca gactttgatc aactcaagca atttctcacc
      781 tttgacaaac aggtccttcg attctatgca atctgggatg atacagacag catgtatggt
      841 gaatgtcgga cctacatcat tcattactat cttatggatg atacggtgga aattcgagag
      901 gtccacgaac ggaatgatgg gagagatcct ttcccactcc taatgaaccg ccagcgtgtg
      961 cccaaagttt tggtggaaaa tgcaaagaac ttccctcagt gtgtgctaga aatctctgac
     1021 caagaagtgt tggaatggta tactgctaaa gacttcattg ttgggaagtc actcactatc
     1081 cttgggagaa ctttcttcat ttatgattgt gatccattta ctcgacggta ttacaaagag
     1141 aagtttggaa tcactgattt accacgtatt gatgtgagca agcgggaacc acctccagta
     1201 aaacaggagt tgcctcctta taacggtttt ggactagtgg aagattctgc tcagaattgt
     1261 tttgctctca ttccaaaagc tccaaaaaaa gacgttatta aaatgctggt gaatgataac
     1321 aaggtgcttc gttatttggc tgtactggaa tcccccatcc cagaagacaa agaccgcaga
     1381 tttgtcttct cttactttct agctaccgac atgatcagta tctttgagcc tcctgttcgc
     1441 aattctggta tcattggggg caagtacctt ggcaggacta aagttgttaa accatactct
     1501 acagtggaca accctgtcta ctatggcccc agtgacttct tcattggtgc tgtgattgaa
     1561 gtgtttggtc accggttcat catccttgat acagacgagt atgttttgaa atacatggag
     1621 agcaacgctg cccagtattc accagaagca ctcgcgtcaa ttcagaacca tgtccgaaag
     1681 cgagaagcgc ctgctccaga agcagaaagc aagcaaactg aaaaggatcc aggcgtgcag
     1741 gaattggaag cattaataga cacaattcag aagcaactga aagatcactc atgcaaagac
     1801 aacattcgtg aggcatttca aatttatgac aaggaagctt caggatatgt ggacagagac
     1861 atgttcttta aaatctgtga atcgcttaac gtcccagtgg atgactcctt ggttaaggag
     1921 ttaatcagga tgtgctctca tggagaaggc aaaattaact actataactt tgttcgtgct
     1981 ttctcaaact gacctgctga tgagaaaatg caagacaatt tttgatactg gaactatgct
     2041 ttgaaataca ccttacactc ttcatagagg catttacagg gttcctgaag ttttatttct
     2101 gttttggttc ttctttcact cctactgaag tcgaaactaa attggatcta ataggatcta
     2161 agattggtgc cttatttagg gtgatagggg tatagcaatg tctaattttg tgtgtcaaat
     2221 tgacttggcc acagggggcc caaatatttc ctttctttct ttttaaaaaa ataaattttt
     2281 ttagagatgg gatctcactc tgtcatacag gctggagtgt ggtggcacta tcctagttct
     2341 atgaagcctc agactcctgg gctcaagtga tcctcccact tcaacctccc cagtagctgg
     2401 gacaacaggc tcaagccacc atgcccagct aatttttaaa attattttgt agagacaagg
     2461 tcttgctagg ttgcctgaac ttgtctcaaa ctcatgagct ccagaaattc tcccacctca
     2521 gcctccaaaa gtgctgggat tataggcatg agccactgtg cccagcctca gatatttcat
     2581 tattctgggg gtttctctga aggtattttt cgatgagata aacatttgaa tcagaagact
     2641 aagtaaaaca gatagtcctc cctaatgtgg gtgggcctcc tccaatcatt tgaagacatg
     2701 agtagaataa aaaggctgac ctttccccag gtaagagaat tcctcctgcc tgactggctt
     2761 caaattggaa catcagcctt ttcttgcctt cagacacaaa tggaaacatc agctgttggt
     2821 ctggagcctg ctgaccttca gactggacct aaaccatcag ctctcctaag tcataggcct
     2881 ttggattcag actggaatta tacctttagc tctcctgggt ctccagcttg ccaatttacc
     2941 ctgcagatct tgggacttgt aagccttcat aattgtgtga gcaatttctt ataactctct
     3001 ctttctctct ctctacatat atatatatat atatatttta tatgtacaca ttcatacaca
     3061 cacacgctta tatgtataca tatagtatgt atatgtgtat atatattatg tatatacata
     3121 tatgtgcaca catctctact aaaaatagaa aaattagcca ggtgtggtgg cgtgtgcctg
     3181 tagtcccagc cactcgggag gcttaaatgg gagaatcact tgaaccctgg aggcagaggt
     3241 tgcagtgagc caagaccacg ccactgcact ccatccagcc tgggtgacag agcaagactc
     3301 catctcaaaa aaaaaaaggt tagaaaaatg ctgcttttag agagcctggc cttgagagag
     3361 gaaggaaaga ctagttgcag ttactattct ctggtgagct ctgactggct ttttacaaac
     3421 agggcttctc tttggttgaa gtcagccact aagttcatct ttaaacatgc tatctcaaat
     3481 tccccgaagc cacccaaaat gtggtatgaa tgaggataga gttacacaga attcagactg
     3541 ctttgccctc tcagaacttc tagggaactt cccttggtgt ttccttccct aacgagctca
     3601 gccacttgta accagcttat tgaaagggct gtgaatgtat tatgttgatt ccttttctgt
     3661 tccaggttat tatcttggga ataacccatg ttttgttttg ttttaccctc agtcgctcat
     3721 aactatttaa aacagtatct ctttcaagta cagatgctcc tctacttatg gggctatgtc
     3781 tcaataaact cacgtaagta aaaaatatca caagttgaaa acacatttaa taaccctgat
     3841 aagcccattg taaagtaggc aaataagttg gaccattgaa agttggggac tatctgtatt
     3901 ttagaagttt tttcagctta aaaattacca agctctatcc tacttggccc agctcagtcc
     3961 ttcccccagg cttagaagcc tgtggtaaag agtgtgtgta tactggggtg atgatagtgg
     4021 tttcttctta ctcccttctt tctgtcttct gctccctttg ttcctattca gccatgctgc
     4081 tgtttctagc ccatgtaggc tctgggaaag gttaagcggg tagaaaccag agaaaaaggg
     4141 caaagtctta tttctgtggc tagtaatgtc gtaatgtggg gccaggtctg agtgacatgt
     4201 gccgaaaggc tgactctttc tcccagagca ccttttctct ctttttggat tcagggggta
     4261 catgtgcagg tttgttacat gagtatattg cacccaggta gtgagcatag tgcccagtaa
     4321 gtagtttttc cacacatacc ccctccttcc cttcccactc tggtagtccc cagtgtctat
     4381 tgttcccatc attatgtcta tgtgtattca atgtttagct cccacttaga agtaagaaca
     4441 tgcaatattt ggtttcctat tcctgcatca attcacttta ggataatggc ctccagctgc
     4501 atccatgttg ctgcagaata catgatttca ttttttatgg ctgcgtagta ttccatggtg
     4561 tagatatact tcatttaaaa aatctaatcc accattgatt ggcacctagg ttgattccat
     4621 gtcctttgct cagagccccg ttttggtgag ttcttagaac tctttccaac cggaaactgc
     4681 ccagtgcacc actctcagat ggacgggacc cagtctgtac ctgatcaccc cggctctaat
     4741 ggtatggtct ccaaggctcc ttctgatctt cccaggaggc cctttcaggc tgacacacct
     4801 tccagggtgt gcactctcgc catgttctca cccagatggc tctcttggct gtgtaattag
     4861 gcagctcaat ccctttcctt tagactgttt caggggagaa ccaggtaccc caaatcttat
     4921 gctctccaaa ctccagggga cagacacatg tcaaaccctg agtcttttaa agtataccct
     4981 cacttagctt gcagacccaa ggcactccag cccctttcaa gaagcttgca ctttctgata
     5041 ttttctccat cactcttgcc tcctgtggta gaggagcttt gggctactcc ttaacaaatc
     5101 attcatggat cggcagcaaa tctgcaacat atggatatat ttgccaattt ttgtcctcag
     5161 ctttgggtct cagccaaaat ggagatttag gaaagtctca tttagcatcc tctagcctgc
     5221 ttttggctgt tttgttttgt ttttgtgttt gttttttaga gacagggtct tactctgttg
     5281 ccagactgga atgcggtggt gtgaccatag ctcactgcag cctcaaactc ctgggctcat
     5341 gcaatcctcc tgcctcagcc ttctgagtag ctaggcctgt agtctcagag ggaactgtct
     5401 tcaataaagt tggcactgtc cacatacact ccttaagttt gctgtccact tcttgccttg
     5461 catctccttt taagtttact taagactgag aagctgagat ctgacagcac caacacaagg
     5521 tataaaagag atttcatgaa agaccaaagc cagcaggcca tttctgtggt gtccaaacac
     5581 tcgctgctca cctgtttata caaagcagca agtataggag ccacaaacgt cagagcaaat
     5641 cactttttct actaaaatcc tgaatggcag tgcagccatt agatgtcact aattacctta
     5701 tgctacagtc atagctggca tgcatctgga gtgtcaggtt caggtggtgg aagggagctg
     5761 agcattaccc tgtagcaagc aatattgtag gaagcagaaa tgatcatcac ttcaaactgg
     5821 ccaccaggga gttgaaagat tctaatacac acacacaccc acacacacac acacacaaag
     5881 agagaatgag aattattaag attagcactg gtagctgaag ctatcaggtc taactgaagc
     5941 atttgactaa gactgcttat agtgcttacg gctttgggac taataaaaat ctgccttgcg
     6001 ggtaaagatg ggctgtgagt tagccactac tcagaaacct gggcctgtac cttgtctgca
     6061 ccatcagggg gccttgaaaa taccatccaa ttaaggtgta tcaaatccat atctataggc
     6121 tttgagggta tttgggacac ataggtagag aagacgtctc taaatgttgt ccagagaatc
     6181 cacttggtta agtgactcct gagtatcctg ctggcacata agttcatttt aaaaagccaa
     6241 tatgagtgct ttatatgtac catgaactta gcattcagta tctctaaatc tctcaccatg
     6301 actccccaca aaacatgaaa cccttttgac acagatgcac aaaaagactc ggaagggtta
     6361 actgcttgag ttcacctagc ttctcatggc agagcaggac ccaactctac aatgaagctt
     6421 gccatgcatc agaaccactc accttcacct caagcaaaca tttcctcttt ctcacacccc
     6481 atgggtgtcc ttgactaact gccacctgta actgcactca tcttttgaga tctttttcca
     6541 aagaaagatg gttctggtaa acacagcaag cagctacagc caacagcagc catgtaagtg
     6601 acaccagcaa tccccactat tactaatact tggatgtctt ctagtcagca ccaacatatt
     6661 tgttggcctt tacacaaacg aaaccccatt tcctccaagc taaataacat catagtatag
     6721 taggcatcaa ggatgatgtg cttgtttctg gagcttccta cccacaaacc ccatgttaag
     6781 aaaacctctt ttccacttta ctattaaaga gtcaaataaa aatgatgctt attgctatcc
     6841 agcacttga
//
