LOCUS       NM_144715               2860 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens EF-hand domain family member B (EFHB), transcript
            variant 1, mRNA.
ACCESSION   NM_144715
VERSION     NM_144715.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Gui,M., Croft,J.T., Zabeo,D., Acharya,V., Kollman,J.M.,
            Burgoyne,T., Hoog,J.L. and Brown,A.
  TITLE     SPACA9 is a lumenal protein of human ciliary singlet and doublet
            microtubules
  JOURNAL   Proc Natl Acad Sci U S A 119 (41), e2207605119 (2022)
   PUBMED   36191189
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Albarran,L., Lopez,J.J., Jardin,I., Sanchez-Collado,J.,
            Berna-Erro,A., Smani,T., Camello,P.J., Salido,G.M. and Rosado,J.A.
  TITLE     EFHB is a Novel Cytosolic Ca2+ Sensor That Modulates STIM1-SARAF
            Interaction
  JOURNAL   Cell Physiol Biochem 51 (3), 1164-1178 (2018)
   PUBMED   30481768
  REMARK    GeneRIF: EFHB is a new store-operated Ca2+ entry regulator that
            modulates STIM1-SARAF interaction.
            GeneRIF: EFHB is a Novel Cytosolic Ca2+ Sensor That Modulates
            STIM1-SARAF Interaction
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC104182.2, AK097644.1 and BC028198.1.
            
            On Nov 22, 2018 this sequence version replaced NM_144715.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC028198.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000295824.14/ ENSP00000295824.9
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AC104182.2         150464-150466       c
            4-200               AC104182.2         150267-150463       c
            201-1189            AK097644.1         200-1188
            1190-1195           AC104182.2         136082-136087       c
            1196-1343           BC028198.1         1159-1306
            1344-1376           AC104182.2         134481-134513       c
            1377-2860           BC028198.1         1340-2823
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p24.3"
     gene            1..2860
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /note="EF-hand domain family member B"
                     /db_xref="GeneID:151651"
                     /db_xref="HGNC:HGNC:26330"
     exon            1..988
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..2701
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /note="isoform 1 is encoded by transcript variant 1;
                     EF-hand domain-containing family member B; cilia and
                     flagella associated protein 21"
                     /codon_start=1
                     /product="EF-hand domain-containing family member B
                     isoform 1"
                     /protein_id="NP_653316.3"
                     /db_xref="CCDS:CCDS33715.2"
                     /db_xref="GeneID:151651"
                     /db_xref="HGNC:HGNC:26330"
                     /translation="MNMEIGHPHEGKDDLGDKRVIMGTKFPMELGIRVGLGKEDSRCG
                     ESPVVSNKCEGRMAPPETKFPLSKGLEMGLERQNISRTVMQRGSLGVDSVSASQGTKP
                     SLLPGRMGLENESLLAGYTHERIIQPPLGRVCGSSQAAGSRRAPLASGPEGVEELVGK
                     PAFVMEPRQEMEKESTCVLMKPNTEIKLPVEVDIGLTQAEGPDETKNTEPQMGLVIEP
                     PQCQFAQQHEQRKEAGNIESGVEPPDRIRPIYSGKFFDRTPCWPSAGKVIPVGYRVAT
                     CLTEKLPRLITPPEAKKYFNFRYPPAGVERVFYGRANDPQIAPYLTHGIRSKISVLAN
                     TLINPQPITTFQQKIKDKKESIYLSNRRAPLGKSHDQAPGLPKGMDTTNTTFGTAVIK
                     EYSAKDVVNPPKSYEEVFKEGNEGHDLYVVSHNDYYAGEAKNRKYNPSSFHRCSVYGV
                     PTPHFNDGRAMAKSLYWLHELQMKRGAKFVSKRADDFKEKFQHKLGRVLDPIAETMNV
                     PPDCTFGACLRPEEYGVGDLIHNRLPDEYLRGKDRQRALIAAVRHHLKKVNYQKFDTL
                     LAAFRHYDKKGDGMIDKDELQEACDQANLSLDDKLLDQLFDYCDVDNDGFINYLEFAN
                     FLNWKDKMLLKEYEERVIIKGRKPDCVNPTEANVEEPEQTLLIKPEDIVLKEAGSTEK
                     TLRTLLRPSDKVSNYYKTTSSEINAIVGAIPSTCYPICGVPTIRSDIPAPRIRRISDR
                     TNYGEEGSAYSLLYPTIFARKGVFERDFFKTRSKEEIAEILCNIGVKLSDEEFENVWN
                     LASKKHHRGEVCVENIRNVLDELRHADRIKCKTLM"
     exon            989..1051
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1195
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1376
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..1487
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /inference="alignment:Splign:2.1.0"
     exon            1488..1617
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /inference="alignment:Splign:2.1.0"
     exon            1618..1701
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /inference="alignment:Splign:2.1.0"
     exon            1702..1769
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /inference="alignment:Splign:2.1.0"
     exon            1770..1924
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /inference="alignment:Splign:2.1.0"
     exon            1925..2132
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2345
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..2527
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /inference="alignment:Splign:2.1.0"
     exon            2528..2860
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2835..2840
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /note="hexamer: AATAAA"
     polyA_site      2860
                     /gene="EFHB"
                     /gene_synonym="CFAP21"
                     /note="major polyA site"
ORIGIN      
        1 agactccatc ttttcctctg tgaactcggc taggtgttaa gaggcaggca ggaagcaatg
       61 gacagggagt ccgagtgagg ttgtgctctt tcctgagaga ggggttccgc aagggattgg
      121 aggcgtcttt gtagccaggt acagctttcc ccttagagat gagcgctctt ggagaaatga
      181 gaatggggag aaatcgtcca tgaacatgga gattggacat ccccacgaag gaaaggatga
      241 tttaggagac aagagggtca tcatgggaac aaaatttccc atggagttgg ggatcagagt
      301 aggattagga aaagaagatt cccgatgcgg ggagagccct gtggttagta ataagtgtga
      361 gggaaggatg gcaccaccag aaacaaaatt tccattgagc aaggggcttg aaatgggatt
      421 agaaagacag aatatttcta ggactgtcat gcagaggggt agtttaggag tcgacagtgt
      481 ctcagcttca cagggaacaa aaccttctct gttgccagga agaatggggt tagaaaatga
      541 gagtcttctt gcaggatata cccatgaacg gataatacag cctcctttgg gcagggtgtg
      601 tggaagttca caggctgcag ggagcaggag agctcctttg gctagtggcc ctgaaggggt
      661 agaggaatta gtgggaaagc ctgctttcgt tatggaacca agacaggaaa tggaaaaaga
      721 gtctacctgt gttttaatga aacccaacac agagattaag cttcctgtgg aggtggacat
      781 tggactaacc caagccgagg ggccagatga gactaagaat acagagcccc aaatgggctt
      841 ggtgatagaa cctccccaat gccagtttgc ccaacaacat gaacagagaa aggaggctgg
      901 aaacattgaa tcaggagtgg aacctccaga tcgcatcaga cccatatact ctgggaagtt
      961 ttttgatcgg accccttgct ggccaagtgc aggaaaagtt attccagttg gttacagagt
     1021 tgcaacctgc ttgactgaaa aacttcccag gctaattact ccacctgaag caaaaaagta
     1081 tttcaacttc agatatccac ctgctggagt agaaagagta ttttacggaa gagcaaatga
     1141 tccccagatt gcaccttatt tgacacatgg aattagatct aaaatttcag tactggcaaa
     1201 cacattgata aacccacagc ctattaccac atttcaacag aaaattaaag ataaaaaaga
     1261 atctatatat cttagcaatc gacgagcacc attaggaaaa tctcacgatc aagcaccagg
     1321 attaccaaaa ggcatggaca caaccaatac gacatttggg acagcagtca tcaaagaata
     1381 ctctgctaaa gatgtggtga atccaccaaa atcctatgaa gaagtattta aagaaggaaa
     1441 tgaaggacat gatttgtatg ttgtttctca caatgattat tatgcaggag aggcaaagaa
     1501 ccgaaagtat aacccatcaa gtttccatag gtgtagtgtg tatggagtac caacaccaca
     1561 ttttaatgat ggacgagcca tggcaaaatc tctatattgg ctccatgaac tacaaatgaa
     1621 aagaggagct aagtttgtat ccaaaagagc agatgatttc aaagaaaagt ttcaacataa
     1681 acttggaaga gttttagatc ccattgcaga aacaatgaat gttcccccag actgcacatt
     1741 tggagcttgt ctccgtcctg aggaatatgg agttggtgat ctcatccata atagacttcc
     1801 ggatgaatat cttcgaggca aggatagaca gcgagccctg attgcagcag ttcggcatca
     1861 cctgaagaaa gttaattacc aaaagtttga cactttgctg gcagccttca ggcactatga
     1921 caagaaggga gatgggatga tagataaaga cgagctgcag gaagcttgtg accaggccaa
     1981 cttgagttta gatgacaagc tcctggacca gctatttgac tactgtgatg tggataatga
     2041 tggcttcatt aactatctgg aattcgcaaa ttttcttaac tggaaagaca aaatgcttct
     2101 taaagagtat gaagagaggg tcattattaa aggtagaaaa ccagattgtg taaaccctac
     2161 tgaggctaat gttgaagaac ctgaacaaac tctcctcata aagccagaag atattgtctt
     2221 aaaagaagca ggaagcacag aaaagactct ccggacactt ctgagaccaa gtgataaagt
     2281 ttccaactac tataagacaa cttcttctga gatcaatgca attgtaggag ccattccttc
     2341 tacttgttac cccatttgtg gtgttccaac cattcgatct gacattcctg ctccccgaat
     2401 tcgtcgcatc agtgacagaa ctaattatgg tgaagaaggt agtgcatatt cactactata
     2461 tcctaccatt tttgcccgga aaggagtgtt tgaaagagac ttcttcaaga ccagatcaaa
     2521 agaagagatt gcagagatat tgtgtaacat tggtgtcaaa ctgtctgatg aagaatttga
     2581 aaatgtatgg aatcttgcat caaaaaagca tcacagagga gaagtttgtg ttgagaacat
     2641 cagaaatgtt ctagatgagc tacggcatgc agaccggatc aagtgtaaaa cactcatgtg
     2701 atatttttgg acttcattca ttcaagcaaa agaattatta actctgtgtt tatctaaaat
     2761 gttgaatcca ttctggtttt agatattatg ttagagttca cagtggtaag actcatatgc
     2821 ctgtatgtgt tgctaataaa ttagattttg gattttaaaa
//
