LOCUS       NM_021937               2205 bp    mRNA    linear   PRI 27-FEB-2025
DEFINITION  Homo sapiens eukaryotic elongation factor, selenocysteine-tRNA
            specific (EEFSEC), mRNA.
ACCESSION   NM_021937
VERSION     NM_021937.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Stolf,C.S., Taiete,T., Nascimento,P.A.D., Paz,H.E.S., Sallum,E.A.,
            Ruiz,K.G.S., Casati,M.Z. and Casarin,R.C.V.
  TITLE     Association of rs142548867 (EEFSEC) and periodontitis Grade C in a
            young Brazilian population
  JOURNAL   J Appl Oral Sci 31, e20230058 (2023)
   PUBMED   37466550
  REMARK    GeneRIF: Association of rs142548867 (EEFSEC) and periodontitis
            Grade C in a young Brazilian population.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Hilal,T., Killam,B.Y., Grozdanovic,M., Dobosz-Bartoszek,M.,
            Loerke,J., Burger,J., Mielke,T., Copeland,P.R., Simonovic,M. and
            Spahn,C.M.T.
  TITLE     Structure of the mammalian ribosome as it decodes the
            selenocysteine UGA codon
  JOURNAL   Science 376 (6599), 1338-1343 (2022)
   PUBMED   35709277
REFERENCE   3  (bases 1 to 2205)
  AUTHORS   Xu,B., Hao,J., Xie,Q., Sa,N., Wang,S., DU,X., Lu,H., Gao,P., Shi,G.
            and Dong,X.
  TITLE     [EEFSEC knockdown inhibits proliferation, migration and invasion of
            prostate cancer cells in vitro]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 41 (12), 1787-1794 (2021)
   PUBMED   35012909
  REMARK    GeneRIF: [EEFSEC knockdown inhibits proliferation, migration and
            invasion of prostate cancer cells in vitro].
REFERENCE   4  (bases 1 to 2205)
  AUTHORS   Zhang,G., Feenstra,B., Bacelis,J., Liu,X., Muglia,L.M.,
            Juodakis,J., Miller,D.E., Litterman,N., Jiang,P.P., Russell,L.,
            Hinds,D.A., Hu,Y., Weirauch,M.T., Chen,X., Chavan,A.R.,
            Wagner,G.P., Pavlicev,M., Nnamani,M.C., Maziarz,J.,
            Karjalainen,M.K., Ramet,M., Sengpiel,V., Geller,F., Boyd,H.A.,
            Palotie,A., Momany,A., Bedell,B., Ryckman,K.K., Huusko,J.M.,
            Forney,C.R., Kottyan,L.C., Hallman,M., Teramo,K., Nohr,E.A., Davey
            Smith,G., Melbye,M., Jacobsson,B. and Muglia,L.J.
  TITLE     Genetic Associations with Gestational Duration and Spontaneous
            Preterm Birth
  JOURNAL   N Engl J Med 377 (12), 1156-1167 (2017)
   PUBMED   28877031
  REMARK    GeneRIF: In this genomewide association study, we found that
            variants at the EBF1, EEFSEC, AGTR2, WNT4, ADCY5, and RAP2C loci
            were associated with gestational duration and variants at the EBF1,
            EEFSEC, and AGTR2 loci with preterm birth.
            GeneRIF: EEFSEC, which encodes selenocysteine-tRNA specific
            eukaryotic elongation factor, participates in the incorporation of
            selenocysteine into selenoproteins.  Selenoproteins serve critical
            cellular homeostatic functions in maintaining redox status and
            antioxidant defenses, as well as modulating inflammatory responses.
            These physiologic functions have been linked to the parturition
            process and preterm birth.
REFERENCE   5  (bases 1 to 2205)
  AUTHORS   Dobosz-Bartoszek,M., Pinkerton,M.H., Otwinowski,Z.,
            Chakravarthy,S., Soll,D., Copeland,P.R. and Simonovic,M.
  TITLE     Crystal structures of the human elongation factor eEFSec suggest a
            non-canonical mechanism for selenocysteine incorporation
  JOURNAL   Nat Commun 7, 12941 (2016)
   PUBMED   27708257
  REMARK    GeneRIF: eEFSec employs a non-canonical mechanism involving the
            distinct C-terminal domain 4 for the release of the
            selenocysteinyl-tRNA during decoding on the ribosome
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2205)
  AUTHORS   Gudmundsson,J., Sulem,P., Gudbjartsson,D.F., Blondal,T.,
            Gylfason,A., Agnarsson,B.A., Benediktsdottir,K.R.,
            Magnusdottir,D.N., Orlygsdottir,G., Jakobsdottir,M., Stacey,S.N.,
            Sigurdsson,A., Wahlfors,T., Tammela,T., Breyer,J.P.,
            McReynolds,K.M., Bradley,K.M., Saez,B., Godino,J., Navarrete,S.,
            Fuertes,F., Murillo,L., Polo,E., Aben,K.K., van Oort,I.M.,
            Suarez,B.K., Helfand,B.T., Kan,D., Zanon,C., Frigge,M.L.,
            Kristjansson,K., Gulcher,J.R., Einarsson,G.V., Jonsson,E.,
            Catalona,W.J., Mayordomo,J.I., Kiemeney,L.A., Smith,J.R.,
            Schleutker,J., Barkardottir,R.B., Kong,A., Thorsteinsdottir,U.,
            Rafnar,T. and Stefansson,K.
  TITLE     Genome-wide association and replication studies identify four
            variants associated with prostate cancer susceptibility
  JOURNAL   Nat Genet 41 (10), 1122-1126 (2009)
   PUBMED   19767754
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
REFERENCE   7  (bases 1 to 2205)
  AUTHORS   Donovan,J., Caban,K., Ranaweera,R., Gonzalez-Flores,J.N. and
            Copeland,P.R.
  TITLE     A novel protein domain induces high affinity selenocysteine
            insertion sequence binding and elongation factor recruitment
  JOURNAL   J Biol Chem 283 (50), 35129-35139 (2008)
   PUBMED   18948268
  REMARK    GeneRIF: SECIS binding induces a conformational change in SBP2 that
            recruits eEFSec, which in concert with the Sec incorporation domain
            gains access to the ribosomal A site
REFERENCE   8  (bases 1 to 2205)
  AUTHORS   Muzny,D.M., Scherer,S.E., Kaul,R., Wang,J., Yu,J., Sudbrak,R.,
            Buhay,C.J., Chen,R., Cree,A., Ding,Y., Dugan-Rocha,S., Gill,R.,
            Gunaratne,P., Harris,R.A., Hawes,A.C., Hernandez,J., Hodgson,A.V.,
            Hume,J., Jackson,A., Khan,Z.M., Kovar-Smith,C., Lewis,L.R.,
            Lozado,R.J., Metzker,M.L., Milosavljevic,A., Miner,G.R.,
            Morgan,M.B., Nazareth,L.V., Scott,G., Sodergren,E., Song,X.Z.,
            Steffen,D., Wei,S., Wheeler,D.A., Wright,M.W., Worley,K.C.,
            Yuan,Y., Zhang,Z., Adams,C.Q., Ansari-Lari,M.A., Ayele,M.,
            Brown,M.J., Chen,G., Chen,Z., Clendenning,J.,
            Clerc-Blankenburg,K.P., Chen,R., Chen,Z., Davis,C., Delgado,O.,
            Dinh,H.H., Dong,W., Draper,H., Ernst,S., Fu,G.,
            Gonzalez-Garay,M.L., Garcia,D.K., Gillett,W., Gu,J., Hao,B.,
            Haugen,E., Havlak,P., He,X., Hennig,S., Hu,S., Huang,W.,
            Jackson,L.R., Jacob,L.S., Kelly,S.H., Kube,M., Levy,R., Li,Z.,
            Liu,B., Liu,J., Liu,W., Lu,J., Maheshwari,M., Nguyen,B.V.,
            Okwuonu,G.O., Palmeiri,A., Pasternak,S., Perez,L.M., Phelps,K.A.,
            Plopper,F.J., Qiang,B., Raymond,C., Rodriguez,R.,
            Saenphimmachak,C., Santibanez,J., Shen,H., Shen,Y., Subramanian,S.,
            Tabor,P.E., Verduzco,D., Waldron,L., Wang,J., Wang,J., Wang,Q.,
            Williams,G.A., Wong,G.K., Yao,Z., Zhang,J., Zhang,X., Zhao,G.,
            Zhou,J., Zhou,Y., Nelson,D., Lehrach,H., Reinhardt,R., Naylor,S.L.,
            Yang,H., Olson,M., Weinstock,G. and Gibbs,R.A.
  TITLE     The DNA sequence, annotation and analysis of human chromosome 3
  JOURNAL   Nature 440 (7088), 1194-1198 (2006)
   PUBMED   16641997
REFERENCE   9  (bases 1 to 2205)
  AUTHORS   Mehta,A., Rebsch,C.M., Kinzy,S.A., Fletcher,J.E. and Copeland,P.R.
  TITLE     Efficiency of mammalian selenocysteine incorporation
  JOURNAL   J Biol Chem 279 (36), 37852-37859 (2004)
   PUBMED   15229221
REFERENCE   10 (bases 1 to 2205)
  AUTHORS   Fagegaltier,D., Hubert,N., Yamada,K., Mizutani,T., Carbon,P. and
            Krol,A.
  TITLE     Characterization of mSelB, a novel mammalian elongation factor for
            selenoprotein translation
  JOURNAL   EMBO J 19 (17), 4796-4805 (2000)
   PUBMED   10970870
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB454660.1, BC007933.2, AL449217.1 and AF268872.1.
            
            On Nov 23, 2018 this sequence version replaced NM_021937.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF268872.1, SRR3476690.956650.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000254730.11/ ENSP00000254730.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-119               DB454660.1         28-146
            120-683             BC007933.2         22-585
            684-684             AL449217.1         43922-43922         c
            685-2201            BC007933.2         587-2103
            2202-2205           AF268872.1         1965-1968
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.3"
     gene            1..2205
                     /gene="EEFSEC"
                     /gene_synonym="EFSEC; NEDPSB; SELB"
                     /note="eukaryotic elongation factor, selenocysteine-tRNA
                     specific"
                     /db_xref="GeneID:60678"
                     /db_xref="HGNC:HGNC:24614"
                     /db_xref="MIM:607695"
     exon            1..343
                     /gene="EEFSEC"
                     /gene_synonym="EFSEC; NEDPSB; SELB"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..1818
                     /gene="EEFSEC"
                     /gene_synonym="EFSEC; NEDPSB; SELB"
                     /note="elongation factor for selenoprotein translation;
                     elongation factor sec; selenocysteine (Sec)-specific
                     eukaryotic elongation factor"
                     /codon_start=1
                     /product="selenocysteine-specific elongation factor"
                     /protein_id="NP_068756.2"
                     /db_xref="CCDS:CCDS33849.1"
                     /db_xref="GeneID:60678"
                     /db_xref="HGNC:HGNC:24614"
                     /db_xref="MIM:607695"
                     /translation="MAGRRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRE
                     RGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRT
                     IIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAI
                     DKMTKKMQKTLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTR
                     DPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMP
                     ITSAMQGDRLGICVTQFDPKLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAK
                     FHITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLTPAVTDND
                     EADKKAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADIHTNTCRLAFHGIL
                     LHGLEDRNYADSFLPRLKVYKLKHKHGLVERAMDDYSVIGRSLFKKETNIQLFVGLKV
                     HLSTGELGIIDSAFGQSGKFKIHIPGGLSPESKKILTPALKKRARAGRGEATRQEESA
                     ERSEPSQHVVLSLTFKRYVFDTHKRMVQSP"
     exon            344..551
                     /gene="EEFSEC"
                     /gene_synonym="EFSEC; NEDPSB; SELB"
                     /inference="alignment:Splign:2.1.0"
     exon            552..648
                     /gene="EEFSEC"
                     /gene_synonym="EFSEC; NEDPSB; SELB"
                     /inference="alignment:Splign:2.1.0"
     exon            649..813
                     /gene="EEFSEC"
                     /gene_synonym="EFSEC; NEDPSB; SELB"
                     /inference="alignment:Splign:2.1.0"
     exon            814..1470
                     /gene="EEFSEC"
                     /gene_synonym="EFSEC; NEDPSB; SELB"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1627
                     /gene="EEFSEC"
                     /gene_synonym="EFSEC; NEDPSB; SELB"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..2205
                     /gene="EEFSEC"
                     /gene_synonym="EFSEC; NEDPSB; SELB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2175..2180
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EEFSEC"
                     /gene_synonym="EFSEC; NEDPSB; SELB"
                     /note="hexamer: AATAAA"
     polyA_site      2205
                     /gene="EEFSEC"
                     /gene_synonym="EFSEC; NEDPSB; SELB"
                     /note="major polyA site"
ORIGIN      
        1 gcgggtgtcc gaggcggcgg cggcggcatg gcagggcggc gggtgaacgt gaacgtgggc
       61 gtgctgggcc acatcgacag cggcaagacg gcgctggcgc gggcgctaag caccacagcc
      121 tccaccgccg cctttgacaa gcagccgcag agccgcgagc gcggcatcac gctcgatctg
      181 ggcttctcgt gcttctcggt gccgctgccc gcgcgcctgc ggtcgtcttt gcccgagttc
      241 caggcagcgc ccgaggccga gcccgagccc ggcgagccac tgcttcaggt cacgctggtc
      301 gactgccccg ggcacgcctc cctcatccgg accatcatcg gcggggccca gatcattgat
      361 ctgatgatgc tggtcatcga tgtgaccaag gggatgcaga cccagtcagc ggaatgcctt
      421 gtgatcggcc agattgcctg ccagaagctg gtcgtggtgc tgaacaaaat agacctctta
      481 cctgaaggaa agagacaggc agcaattgat aaaatgacca agaaaatgca gaagacccta
      541 gagaacacca agttccgagg tgcaccgatt atacccgtgg cggccaagcc ggggggacca
      601 gaggcccccg aaactgaagc tccacagggc attccagagc tcattgagct cctgacgtcc
      661 cagatttcca tcccaacgag agatccctcg ggaccgttcc tcatgtctgt ggaccactgt
      721 ttctccatca aaggccaagg cactgtgatg acagggacca tcctttcagg ctccatcagc
      781 ctcggtgaca gtgtggagat ccctgccctc aaggtggtga agaaggtgaa gtccatgcag
      841 atgttccaca tgcccatcac ttcagccatg caaggagacc ggctgggcat ctgcgtcacc
      901 cagtttgacc ctaagctgct ggagcgcggg ttggtgtgtg cccccgagtc cctgcacact
      961 gtccatgcgg ccctcatctc tgtggaaaag ataccgtatt tccgggggcc cctgcaaacc
     1021 aaggccaagt tccacattac agtgggccat gaaacagtca tgggccggtt gatgttcttc
     1081 agtcctgctc cagataactt tgaccaggag cctatactgg actctttcaa cttctctcaa
     1141 gaataccttt tccaggagca gtacctgtcc aaggatttga caccagcagt gacagacaat
     1201 gatgaggccg acaagaaggc cggccaggcc acagagggcc attgtcctcg gcagcagtgg
     1261 gccctggtgg agtttgagaa gcccgtcacc tgccctcggc tgtgcctggt gattggctcc
     1321 aggctagatg cggacattca caccaacacg tgccggctag ccttccatgg catcctgctc
     1381 cacgggctag aggacaggaa ctacgccgac agcttcctgc ccaggctgaa ggtgtacaag
     1441 ctgaagcaca agcatggcct tgtggagcgg gcgatggatg actacagtgt gatcggccgc
     1501 tccctgttca aaaaggaaac caacatccag ctcttcgtgg ggctcaaggt gcacttgtcc
     1561 actggggaac tgggcatcat cgacagtgcc ttcggccaga gcggcaagtt caagatccac
     1621 atcccaggtg gcctcagccc cgagtccaag aagatcctga cacccgccct caagaagcgg
     1681 gcccgggctg gccgtgggga ggccaccagg caggaggaga gcgccgagcg gagcgagccc
     1741 tcacagcatg tggtgctcag cctgactttc aagcgttatg tcttcgacac ccacaagcgc
     1801 atggttcagt ctccctgagt gtccggtgac ctcccccagg gcctccttgc ccagcccagt
     1861 ccaggctgct gtgccaaatc ccaaccagcc acgcctcagc ctctcccagt ctctccctgc
     1921 agtcctgcag cagcagcccc cacccccaag cttggtgctg agccctggtg aggagctgag
     1981 ggggatgggt tgctggggcc aggagggtct ctcctccagc ccctgcacac tcccacccag
     2041 gacagccccc agcccaacta ggaaagggcc atgggcagag ggctggtagc cagtatcttc
     2101 cactgcccca tctgttggcc acctgcaggc cagtctcaac cctcccccag gtgggcaggc
     2161 acttgatggc tacaaataaa tgtcccgtgg ccccagccca ctcta
//
