LOCUS       NM_013302               7398 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), mRNA.
ACCESSION   NM_013302
VERSION     NM_013302.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Ma,T.
  TITLE     Roles of eukaryotic elongation factor 2 kinase (eEF2K) in neuronal
            plasticity, cognition, and Alzheimer disease
  JOURNAL   J Neurochem 166 (1), 47-57 (2023)
   PUBMED   34796967
  REMARK    GeneRIF: Roles of eukaryotic elongation factor 2 kinase (eEF2K) in
            neuronal plasticity, cognition, and Alzheimer disease.
            Review article
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Piserchio,A., Isiorho,E.A., Dalby,K.N. and Ghose,R.
  TITLE     Structure of the complex between calmodulin and a functional
            construct of eukaryotic elongation factor 2 kinase bound to an
            ATP-competitive inhibitor
  JOURNAL   J Biol Chem 299 (6), 104813 (2023)
   PUBMED   37172726
  REMARK    GeneRIF: Structure of the complex between calmodulin and a
            functional construct of eukaryotic elongation factor 2 kinase bound
            to an ATP-competitive inhibitor.
REFERENCE   3  (bases 1 to 7398)
  AUTHORS   Chen,X., Wang,K., Jiang,S., Sun,H., Che,X., Zhang,M., He,J.,
            Wen,Y., Liao,M., Li,X., Zhou,X., Song,J., Ren,X., Yi,W., Yang,J.,
            Chen,X., Yin,M. and Cheng,Y.
  TITLE     eEF2K promotes PD-L1 stabilization through inactivating GSK3beta in
            melanoma
  JOURNAL   J Immunother Cancer 10 (3) (2022)
   PUBMED   35347072
  REMARK    GeneRIF: eEF2K promotes PD-L1 stabilization through inactivating
            GSK3beta in melanoma.
REFERENCE   4  (bases 1 to 7398)
  AUTHORS   Gurbuz,N., Kahraman,N., Sonmez,H.E., Mokhlis,H.A., Kosar,P.A. and
            Ozpolat,B.
  TITLE     miRNA-193b-5p Suppresses Pancreatic Cancer Cell Proliferation,
            Invasion, Epithelial Mesenchymal Transition, and Tumor Growth by
            Inhibiting eEF2K
  JOURNAL   Anticancer Agents Med Chem 22 (14), 2607-2618 (2022)
   PUBMED   35718922
  REMARK    GeneRIF: miRNA-193b-5p Suppresses Pancreatic Cancer Cell
            Proliferation, Invasion, Epithelial Mesenchymal Transition, and
            Tumor Growth by Inhibiting eEF2K.
REFERENCE   5  (bases 1 to 7398)
  AUTHORS   Jiang,M., Qi,L., Jin,K., Li,L., Wu,Y., Song,D., Gan,J., Huang,M.,
            Li,Y. and Song,C.
  TITLE     eEF2K as a novel metastatic and prognostic biomarker in gastric
            cancer patients
  JOURNAL   Pathol Res Pract 225, 153568 (2021)
   PUBMED   34352439
  REMARK    GeneRIF: eEF2K as a novel metastatic and prognostic biomarker in
            gastric cancer patients.
REFERENCE   6  (bases 1 to 7398)
  AUTHORS   Diggle,T.A., Subkhankulova,T., Lilley,K.S., Shikotra,N.,
            Willis,A.E. and Redpath,N.T.
  TITLE     Phosphorylation of elongation factor-2 kinase on serine 499 by
            cAMP-dependent protein kinase induces Ca2+/calmodulin-independent
            activity
  JOURNAL   Biochem J 353 (Pt 3), 621-626 (2001)
   PUBMED   11171059
REFERENCE   7  (bases 1 to 7398)
  AUTHORS   Pavur,K.S., Petrov,A.N. and Ryazanov,A.G.
  TITLE     Mapping the functional domains of elongation factor-2 kinase
  JOURNAL   Biochemistry 39 (40), 12216-12224 (2000)
   PUBMED   11015200
REFERENCE   8  (bases 1 to 7398)
  AUTHORS   Ryazanov,A.G., Ward,M.D., Mendola,C.E., Pavur,K.S., Dorovkov,M.V.,
            Wiedmann,M., Erdjument-Bromage,H., Tempst,P., Parmer,T.G.,
            Prostko,C.R., Germino,F.J. and Hait,W.N.
  TITLE     Identification of a new class of protein kinases represented by
            eukaryotic elongation factor-2 kinase
  JOURNAL   Proc Natl Acad Sci U S A 94 (10), 4884-4889 (1997)
   PUBMED   9144159
REFERENCE   9  (bases 1 to 7398)
  AUTHORS   Redpath,N.T., Price,N.T., Severinov,K.V. and Proud,C.G.
  TITLE     Regulation of elongation factor-2 by multisite phosphorylation
  JOURNAL   Eur J Biochem 213 (2), 689-699 (1993)
   PUBMED   8386634
REFERENCE   10 (bases 1 to 7398)
  AUTHORS   Nairn,A.C. and Palfrey,H.C.
  TITLE     Identification of the major Mr 100,000 substrate for
            calmodulin-dependent protein kinase III in mammalian cells as
            elongation factor-2
  JOURNAL   J Biol Chem 262 (36), 17299-17303 (1987)
   PUBMED   3693353
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009034.10.
            
            On May 21, 2020 this sequence version replaced NM_013302.4.
            
            Summary: This gene encodes a highly conserved protein kinase in the
            calmodulin-mediated signaling pathway that links activation of cell
            surface receptors to cell division. This kinase is involved in the
            regulation of protein synthesis. It phosphorylates eukaryotic
            elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The
            activity of this kinase is increased in many cancers and may be a
            valid target for anti-cancer treatment. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.3774843.1,
                                           SRR11853566.10028.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263026.10/ ENSP00000263026.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-402               AC009034.10        70070-70471
            403-724             AC009034.10        89446-89767
            725-825             AC009034.10        108422-108522
            826-886             AC009034.10        112547-112607
            887-924             AC009034.10        114446-114483
            925-1096            AC009034.10        114943-115114
            1097-1246           AC009034.10        120540-120689
            1247-1379           AC009034.10        121045-121177
            1380-1507           AC009034.10        121435-121562
            1508-1709           AC009034.10        122286-122487
            1710-1777           AC009034.10        124254-124321
            1778-1855           AC009034.10        126902-126979
            1856-1918           AC009034.10        128610-128672
            1919-2053           AC009034.10        130182-130316
            2054-2242           AC009034.10        130480-130668
            2243-2367           AC009034.10        137418-137542
            2368-2546           AC009034.10        143990-144168
            2547-7398           AC009034.10        147679-152530
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p12.2"
     gene            1..7398
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /note="eukaryotic elongation factor 2 kinase"
                     /db_xref="GeneID:29904"
                     /db_xref="HGNC:HGNC:24615"
                     /db_xref="MIM:606968"
     exon            1..402
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            403..724
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     CDS             479..2656
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /EC_number="2.7.11.20"
                     /note="calmodulin-dependent protein kinase III; elongation
                     factor-2 kinase; calcium/calmodulin-dependent eukaryotic
                     elongation factor-2 kinase; eEF-2 kinase; eukaroytic
                     elongation factor 2 kinase; alternative protein EEF2K"
                     /codon_start=1
                     /product="eukaryotic elongation factor 2 kinase"
                     /protein_id="NP_037434.2"
                     /db_xref="CCDS:CCDS10604.1"
                     /db_xref="GeneID:29904"
                     /db_xref="HGNC:HGNC:24615"
                     /db_xref="MIM:606968"
                     /translation="MADEDLIFRLEGVDGGQSPRAGHDGDSDGDSDDEEGYFICPITD
                     DPSSNQNVNSKVNKYYSNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEF
                     HLEDIATERATRHRYNAVTGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQ
                     WKGASNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELK
                     DRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQ
                     GVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICESMGLAPFDLSPRERD
                     AVNQNTKLLQSAKTILRGTEEKCGSPQVRTLSGSRPPLLRPLSENSGDENMSDVTFDS
                     LPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGE
                     LDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQR
                     LNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEA
                     IVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQ
                     NLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGL
                     EKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE"
     exon            725..825
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            826..886
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            887..924
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1096
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1246
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1379
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1507
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1709
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..1777
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            1778..1855
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            1856..1918
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            1919..2053
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            2054..2242
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            2243..2367
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            2368..2546
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     exon            2547..7398
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5272..5277
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /note="hexamer: AATAAA"
     polyA_site      5293
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /note="major polyA site"
     regulatory      7374..7379
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
                     /note="hexamer: AATAAA"
     polyA_site      7398
                     /gene="EEF2K"
                     /gene_synonym="CaMKIII; eEF-2K; HSU93850"
ORIGIN      
        1 gctcaaccta gaccagcccc agcttcagcc tcagctcccc tccttcctgg atcgagcgcc
       61 cgcactcccg gccctgcagc cacccgagtc ccgctcgctg tcgcctgcac gcgagtcccc
      121 cctggcacgc gctcccacat cccgggatcg tcccaacggc ccctgcgccc ttcctgggat
      181 cactccgact gccccgcgcg ccctgggatc ggtccatcta ccccgcgtgg ccccagctgc
      241 ttgcccggag cgccagctag cgctccccgc tctccgctcc ccggcactct cggggggccc
      301 gcccgccctg caccctggag ctccgggccg cgagcctctg ccaactcctc tggaccctcg
      361 cggccgtggg cagcggctgc cgcgcctgtc tgcccgaggg aggaccttcg cctctgcatt
      421 tgtccagtaa ctctggctgt gccggatact gcttgggtaa aacgggcacc ccaggaacat
      481 ggcagacgaa gatctcatct tccgcctgga aggcgttgat ggcggccagt ccccccgagc
      541 tggccatgat ggtgattctg atggggacag cgacgatgag gaaggttact tcatctgccc
      601 catcacggat gacccaagct cgaaccagaa tgtcaattcc aaggttaata agtactacag
      661 caacctaaca aaaagtgagc ggtatagctc cagcgggtcc ccggcaaact ccttccactt
      721 caaggaagcc tggaagcacg caatccagaa ggccaagcac atgcccgacc cctgggctga
      781 gttccacctg gaagatattg ccaccgaacg tgctactcga cacaggtaca acgccgtcac
      841 cggggaatgg ctggatgatg aagttctgat caagatggca tctcagccct tcggccgagg
      901 agcaatgagg gagtgcttcc ggacgaagaa gctctccaac ttcttgcatg cccagcagtg
      961 gaagggcgcc tccaactacg tggcgaagcg ctacatcgag cccgtagacc gggatgtgta
     1021 ctttgaggac gtgcgtctac agatggaggc caagctctgg ggggaggagt ataatcggca
     1081 caagcccccc aagcaggtgg acatcatgca gatgtgcatc atcgagctga aggacagacc
     1141 gggcaagccc ctcttccacc tggagcacta catcgagggc aagtacatca agtacaactc
     1201 caactctggc tttgtccgcg atgacaacat ccgcctgacg ccgcaggcct tcagccactt
     1261 cacttttgag cgttccggcc atcagctgat agtggtggac atccagggag ttggggatct
     1321 ctacactgac ccacagatcc acacggagac gggcactgac tttggagacg gcaacctagg
     1381 tgtccgcggg atggcgctct tcttctactc tcatgcctgc aaccggattt gcgagagcat
     1441 gggccttgct ccctttgacc tctcgccccg ggagagggat gcagtgaatc agaacaccaa
     1501 gctgctgcaa tcagccaaga ccatcttgag aggaacagag gaaaaatgtg ggagccccca
     1561 agtaaggacc ctctctggga gccggccacc cctgctccgt cccctttcag agaactctgg
     1621 agacgagaac atgagcgacg tgaccttcga ctctctccct tcttccccat cttcggccac
     1681 accacacagc cagaagctag accacctcca ttggccagtg ttcagtgacc tcgataacat
     1741 ggcatccaga gaccatgatc atctagacaa ccaccgggag tctgagaata gtggggacag
     1801 cggatacccc agtgagaagc ggggtgagct ggatgaccct gagccccgag aacatggcca
     1861 ctcatacagt aatcggaagt acgagtctga cgaagacagc ctgggcagct ctggacgggt
     1921 atgtgtagag aagtggaatc tcctcaactc ctcccgcctc cacctgccga gggcttcggc
     1981 cgtggccctg gaagtgcaaa ggcttaatgc tctggacctc gaaaagaaaa tcgggaagtc
     2041 cattttgggg aaggtccatc tggccatggt gcgctaccac gagggtgggc gcttctgcga
     2101 gaagggcgag gagtgggacc aggagtcggc tgtcttccac ctggagcacg cagccaacct
     2161 gggcgagctg gaggccatcg tgggcctggg actcatgtac tcgcagttgc ctcatcacat
     2221 cctagccgat gtctctctga aggagacaga agagaacaaa accaaaggat ttgattactt
     2281 actaaaggcc gctgaagctg gcgacaggca gtccatgatc ctagtggcgc gagcttttga
     2341 ctctggccag aacctcagcc cggacaggtg ccaagactgg ctagaggccc tgcactggta
     2401 caacactgcc ctggagatga cggactgtga tgagggcggt gagtacgacg gaatgcagga
     2461 cgagccccgg tacatgatgc tggccaggga ggccgagatg ctgttcacag gaggctacgg
     2521 gctggagaag gacccgcaga gatcagggga cttgtatacc caggcagcag aggcagcgat
     2581 ggaagccatg aagggccgac tggccaacca gtactaccaa aaggctgaag aggcctgggc
     2641 ccagatggag gagtaaccag gaaaatcact gccggctagt cccaagcaaa cgggctagga
     2701 ggaaagatta aaaaaacaac aacaacaact tatttagttt ggggagggga agcattttta
     2761 agtgtgttgt aaaatcaaat tttatatttc attttttgac tcttgaaaaa tgtctttgct
     2821 ccttggcagc taccagcaga gactctatag ctgtctctta gggcagtatt ttggggaagt
     2881 ggggcttgaa gaagcagcct aatgaaccaa cataccgttt tgtgtgtggt tttttttgtt
     2941 tgtttgtttg tttgttttga gacagagtct tgctctgtca cccaggctgg agtgcagtga
     3001 catgatctta gctcactgca acctccgcct cctgggttca agtgattctc ctgcctcagc
     3061 ctcccaagta gctgggatta ctggtgcaca ccaccacact cagctaattt ttgcattttt
     3121 agtagagatg gggtttcacc atgttggcca ggctggtctc gaactcctaa cctcaggtga
     3181 tccacctgcc tcagcctccc aaagtgctgg gattacaggt gtgagccacc atgcctgccc
     3241 attttgtggt tctattttca tttttatttc tttttttttt tttgtcacga gatataagaa
     3301 agtgcttttt gccttgaatg gacaatttta gggctgtgct cactagtctt ttcaggctgg
     3361 actgaaatgt cgggcccatg gagccctgtg ttttgtgcat cgggatgaga aatgaagcac
     3421 ttcacgctgg ctttcctaag tcacggggcg tgtattgccg tggcttagtg caaagcattc
     3481 tttctcagag catttagagg catgcgtggc attttttcag tgggtgtgag attgcacaat
     3541 acccaggctc ccttctactg tggggaaggg cctgcatgtt ggctgttttt taaacttcta
     3601 gttcaatttc cttccataat gctactgatt ttctggcata cagccgaatt ccatctttta
     3661 agcatgcttt ctacggtggg cttttcaaaa caggtttgag ttttgtatgc acacgtttac
     3721 tacctctaac tcctacatca gctagtgtgg aagagggtgc acctcaaagc ttttacacgt
     3781 aaggacagcg gcttggaatg tgagagcctt ttctccaagc agacccacac tctgcatctc
     3841 agtggcagct cccacaacgt gactgcaatg tctcttatac agtattcctt ggtgttttct
     3901 tagtgtctgg atgttcttac gtgaaatctg ctccccagcc ctggtccttg gcattttctg
     3961 cttgaagctg ggctgatttt cttgtaattt acagcaggac gctttcagca gcagtctctt
     4021 gggattttat ctaagatgtt tgaggatgag agggcaagaa ctataaacac tcattaattc
     4081 tagtagtctc cccatggcca gacaatggcg attgttattt aatgagcttt tcctttcaat
     4141 ggaattcagc tctcacatta gtatgatttc atttgatgtt tcaaatagca aagatgctag
     4201 gtgcggtggc tcccgcctgt aatctcagca ctttgaggag ggccaaggtg ggaggattgc
     4261 ttgagctcag gacttcaaga ccagcctggg taaacatggc gagaccctgt ctctaccaaa
     4321 acaacaaaaa aaagacagac tgctttgatc aaccctaaat gcaaaagcag cctatttttc
     4381 tttgtttaaa agtcaaaaca taaaaaagca gagtataaca tacaaaccat tcttaactat
     4441 tcattaaaat gggtccttca acaccttagt ggggtttgtt gttgttgctt atgcagagag
     4501 attattttct ttttattatt ttataatttt tgaaatagag atggggtctc actgtgttgc
     4561 ccaggctggt ctcgaactcc tggacttaag tgagcctccc gcctcagtct cccaaagcgc
     4621 tgggattaca ggcaggagcc actgagccca gccaagactt cagtgttgac tgctttggag
     4681 gcacaaaccc atgcaagcgt tagttccaaa gttcagtgtg tacccttaaa tgaacaatga
     4741 agcaggtaaa attacccttg aaaaaaatcc cttggaccac ccataaatga cagtgacttt
     4801 ttcaatatgg actcatcata gccagttttc cttttgaagt tggaactgat cacccttttg
     4861 tcatctgtac cagatcagta gttggcttgt gttacatttt gtgtgtgtgt gtgcgtgttt
     4921 taaaccagtg catataaatt gtatgttaaa tgtaagtaac tttaagttga cttatctctt
     4981 cacagtaatc aagcctcacg taattcatgc tttttaaatt cagccagccc cccctctctg
     5041 aaattttatt atgtaaataa tttgtgttcc ctgatcactc gtttaagttc ttagttgtat
     5101 gtcatctctt ctctagcagg aattggcaaa cttttttgta aaggggtaga aagtgaagat
     5161 tttaggcttt gcaggccata tagcctctgc tgcaaatgct cagccctgct gttgtaatgt
     5221 aaaagctgcc acagacacta catgaacacg aatgagtgtg gctggtgttc caataaaact
     5281 ttatttacac aaacgggtgg cccttttggg ctgtagtttg tcaacccttg ctctaaccct
     5341 tgactgagag ctactttatt aagccctgag ggcaggagct atcccaattt tgtgttcccc
     5401 agggcaccaa aacacagtgc tttggcatag agtaggcact caacaagtgt gtgaacagat
     5461 ggagagccag ccctagtcag tgcactcacc ctttgaggct ctggttcctc caaacaatga
     5521 ttcgttgtct ggattggctg gaactgtcac ccccgcaatt ctactcccca cccacccatg
     5581 tgaccttaat gtcagttgct ggtctgttgc tcttcgggga gggagagatg gcctggatac
     5641 agagctaagc aaatgcttct ttaagggccc ttaaaagtga aaagtaactt gcaagaggtt
     5701 gagatccttc tgagctagga gaacttattc caccttcaaa acctagtttg ggctgggtgc
     5761 agtggctcac gccactttgg gaggctgagg tgggcggatc acctgaagtc agctactcgg
     5821 gaggctgagg caggagaatc gcttgaaccc aggaggcaga ggttgcagtg agccgagatt
     5881 gcgtcactgc actccagcct gggcgacaga gtgagactcc gtctcgaaac caaaaacaag
     5941 aaaaacccct agttttgtgc cttctgaaga tagttaggac atcttctttt tccgcaaatg
     6001 ctggacatgc caaaaaccta acgcaaaacc caggacattc cagcccaact gggacgatat
     6061 cagagaccat cttcaagtgc aagaagcagg tgaaagccca ggaaattggc agagccagga
     6121 ttatcatcca gtactttcat ttcacaaatg gagaaaccga ggttctgcca gctagatttt
     6181 ttttcacagt gtcactgcta gcaagccact aagctggagc tgggatttga gagctgctgc
     6241 tatttagagg atcttgggaa taaaatttaa actggagttt aatggccctt tcagttttgc
     6301 tataggcaag agaataaaat gaatgaatgg ataggtggct ttatgggtgt aagaaagaag
     6361 cgaaaaaaac tcccaaaccc cagtgttcct gaatatctgt tctccccctg accactctgg
     6421 gaatttatca aatgcagctt tgacctccaa gccaagtaaa ctgctcttgt tgctatttta
     6481 gtggtttttg tttttttaag acacaaggtc tcactttgtt actcaggctg gagtgcagtg
     6541 gcatgatcat aactcattgt aggctcaacc tcctgggccc cagtgatcct cctgcctcag
     6601 cctctcaagt agctaaaact acagatgtgc accatcacat ctggctaatt tttttttttt
     6661 tttttttgag gtggagtctc gctctgttgc ccagattcaa gtgcaatggc acgttttggc
     6721 tcactgcaac ctctgcctcc caggttcaag cgattctccc tgtctcggcc tcccgagtag
     6781 ctgggactac aggcacctgc caccacgcgc agctaatgtt tgtattttta gtagagacgg
     6841 gggtttcacc atgttggcca ggctggtttc aaactcctga cattaggtga tccacctgcc
     6901 tcggcctccc aaagtgctgg gattacaggc atgagccacc gtgccaagcc acgcctggct
     6961 aattttttaa aattattttt tgcagagaca gagtttcact atgttgccca ggctggtctt
     7021 gaactccttg gcctcaagtg atcctcccac ctcagcctcc caaagcattg gggttacagg
     7081 cgtgagccac tgcacctggc cctgttttca tggcttttat ttcctttcat cccacacatt
     7141 tgtctgcagt actagacatg ttttacaaat caacaagttt acacaagtat atgcagcttg
     7201 ttttgggggg aaaggagaca aaatatgcat attttctgtg tggatttgtg cctcttaatt
     7261 gtgtttctag tctctaaggt gaccctttaa cctactcaag atggggccca gagaagtggc
     7321 ctgcgttaca gatttatttt ggcatatgta ctaagttcca ttttctcttt acaaataaag
     7381 tgttttcttt cttttctg
//
