LOCUS       NM_001961               3158 bp    mRNA    linear   PRI 07-OCT-2024
DEFINITION  Homo sapiens eukaryotic translation elongation factor 2 (EEF2),
            mRNA.
ACCESSION   NM_001961
VERSION     NM_001961.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Francis,J.W., Shao,Z., Narkhede,P., Trinh,A.T., Lu,J., Song,J. and
            Gozani,O.
  TITLE     The FAM86 domain of FAM86A confers substrate specificity to promote
            EEF2-Lys525 methylation
  JOURNAL   J Biol Chem 299 (7), 104842 (2023)
   PUBMED   37209825
  REMARK    GeneRIF: The FAM86 domain of FAM86A confers substrate specificity
            to promote EEF2-Lys525 methylation.
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Raspin,K., Marthick,J.R., Donovan,S., Blizzard,L., Malley,R.C.,
            Jung,C.H., Banks,A., Redwig,F., Skala,M., Dickinson,J.L. and
            FitzGerald,L.M.
  TITLE     Identification of a novel recurrent EEF2 gene amplification in
            familial prostate tumors
  JOURNAL   Genes Chromosomes Cancer 62 (5), 247-255 (2023)
   PUBMED   36520140
  REMARK    GeneRIF: Identification of a novel recurrent EEF2 gene
            amplification in familial prostate tumors.
REFERENCE   3  (bases 1 to 3158)
  AUTHORS   Chen,J., Wang,J., Wu,X., Simon,N., Svensson,C.I., Yuan,J.,
            Hart,D.A., Ahmed,A.S. and Ackermann,P.W.
  TITLE     eEF2 improves dense connective tissue repair and healing outcome by
            regulating cellular death, autophagy, apoptosis, proliferation and
            migration
  JOURNAL   Cell Mol Life Sci 80 (5), 128 (2023)
   PUBMED   37084140
  REMARK    GeneRIF: eEF2 improves dense connective tissue repair and healing
            outcome by regulating cellular death, autophagy, apoptosis,
            proliferation and migration.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3158)
  AUTHORS   Liu,S., Matsui,T.S., Kang,N. and Deguchi,S.
  TITLE     Analysis of senescence-responsive stress fiber proteome reveals
            reorganization of stress fibers mediated by elongation factor eEF2
            in HFF-1 cells
  JOURNAL   Mol Biol Cell 33 (1), ar10 (2022)
   PUBMED   34705524
  REMARK    GeneRIF: Analysis of senescence-responsive stress fiber proteome
            reveals reorganization of stress fibers mediated by elongation
            factor eEF2 in HFF-1 cells.
REFERENCE   5  (bases 1 to 3158)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 3158)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
REFERENCE   7  (bases 1 to 3158)
  AUTHORS   Hanes,J., Freudenstein,J., Rapp,G. and Scheit,K.H.
  TITLE     Construction of a plasmid containing the complete coding region of
            human elongation factor 2
  JOURNAL   Biol Chem Hoppe Seyler 373 (4), 201-204 (1992)
   PUBMED   1596361
REFERENCE   8  (bases 1 to 3158)
  AUTHORS   Nygard,O. and Nilsson,L.
  TITLE     Kinetic determination of the effects of ADP-ribosylation on the
            interaction of eukaryotic elongation factor 2 with ribosomes
  JOURNAL   J Biol Chem 265 (11), 6030-6034 (1990)
   PUBMED   2318846
REFERENCE   9  (bases 1 to 3158)
  AUTHORS   Rapp,G., Klaudiny,J., Hagendorff,G., Luck,M.R. and Scheit,K.H.
  TITLE     Complete sequence of the coding region of human elongation factor 2
            (EF-2) by enzymatic amplification of cDNA from human ovarian
            granulosa cells
  JOURNAL   Biol Chem Hoppe Seyler 370 (10), 1071-1075 (1989)
   PUBMED   2610926
REFERENCE   10 (bases 1 to 3158)
  AUTHORS   Rapp,G., Mucha,J., Einspanier,R., Luck,M. and Scheit,K.H.
  TITLE     Cloning and sequence analysis of a cDNA from human ovarian
            granulosa cells encoding the C-terminal part of human elongation
            factor 2
  JOURNAL   Biol Chem Hoppe Seyler 369 (4), 247-250 (1988)
   PUBMED   2840927
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Francesco Amaldi. The reference sequence was
            derived from AU118535.1, AY942181.1 and Z11692.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001961.3.
            
            Summary: This gene encodes a member of the GTP-binding translation
            elongation factor family. This protein is an essential factor for
            protein synthesis. It promotes the GTP-dependent translocation of
            the nascent protein chain from the A-site to the P-site of the
            ribosome. This protein is completely inactivated by EF-2 kinase
            phosporylation. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1087050.1,
                                           SRR3476690.957920.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2142670
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000309311.7/ ENSP00000307940.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-86                AU118535.1         1-86
            87-2218             AY942181.1         4-2135
            2219-3158           Z11692.1           2136-3075
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..3158
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /note="eukaryotic translation elongation factor 2"
                     /db_xref="GeneID:1938"
                     /db_xref="HGNC:HGNC:3214"
                     /db_xref="MIM:130610"
     exon            1..86
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..2660
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /note="polypeptidyl-tRNA translocase; epididymis secretory
                     sperm binding protein"
                     /codon_start=1
                     /product="elongation factor 2"
                     /protein_id="NP_001952.1"
                     /db_xref="CCDS:CCDS12117.1"
                     /db_xref="GeneID:1938"
                     /db_xref="HGNC:HGNC:3214"
                     /db_xref="MIM:130610"
                     /translation="MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAG
                     IIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGAGFLINL
                     IDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKM
                     DRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGL
                     HGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFSKS
                     ATSPEGKKLPRTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKP
                     LLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKG
                     PLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRT
                     ILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVA
                     VEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHA
                     CIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSA
                     RQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAG
                     FQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRL
                     MEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTA
                     DLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLD
                     KL"
     exon            87..301
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     exon            302..483
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     exon            484..695
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     exon            696..874
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     exon            875..980
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1094
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1233
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1429
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1688
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     exon            1689..1796
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..2150
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     exon            2151..2333
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     exon            2334..2466
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     exon            2467..3158
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3137..3142
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /note="hexamer: AATAAA"
     polyA_site      3158
                     /gene="EEF2"
                     /gene_synonym="EEF-2; EF-2; EF2; SCA26"
                     /note="major polyA site"
ORIGIN      
        1 ctcttccgcc gtcgtcgccg ccatcctcgg cgcgactcgc ttctttcggt tctacctggg
       61 agaatccacc gccatccgcc accatggtga acttcacggt agaccagatc cgcgccatca
      121 tggacaagaa ggccaacatc cgcaacatgt ctgtcatcgc ccacgtggac catggcaagt
      181 ccacgctgac agactccctg gtgtgcaagg cgggcatcat cgcctcggcc cgggccgggg
      241 agacacgctt cactgatacc cggaaggacg agcaggagcg ttgcatcacc atcaagtcaa
      301 ctgccatctc cctcttctac gagctctcgg agaatgactt gaacttcatc aagcagagca
      361 aggacggtgc cggcttcctc atcaacctca ttgactcccc cgggcatgtc gacttctcct
      421 cggaggtgac tgctgccctc cgagtcaccg atggcgcatt ggtggtggtg gactgcgtgt
      481 caggcgtgtg cgtgcagacg gagacagtgc tgcggcaggc cattgccgag cgcatcaagc
      541 ctgtgctgat gatgaacaag atggaccgcg ccctgctgga gctgcagctg gagcccgagg
      601 agctctacca gactttccag cgcatcgtgg agaacgtgaa cgtcatcatc tccacctacg
      661 gcgagggcga gagcggcccc atgggcaaca tcatgatcga tcctgtcctc ggtaccgtgg
      721 gctttgggtc tggcctccac gggtgggcct tcaccctgaa gcagtttgcc gagatgtatg
      781 tggccaagtt cgccgccaag ggggagggcc agttggggcc tgccgagcgg gccaagaaag
      841 tagaggacat gatgaagaag ctgtggggtg acaggtactt tgacccagcc aacggcaagt
      901 tcagcaagtc agccaccagc cccgaaggga agaagctgcc acgcaccttc tgccagctga
      961 tcctggaccc catcttcaag gtgtttgatg cgatcatgaa tttcaagaaa gaggagacag
     1021 caaaactgat agagaaactg gacatcaaac tggacagcga ggacaaggac aaagaaggca
     1081 aacccctgct gaaggctgtg atgcgccgct ggctgcctgc cggagacgcc ttgttgcaga
     1141 tgatcaccat ccacctgccc tcccctgtga cggcccagaa gtaccgctgc gagctcctgt
     1201 acgaggggcc cccggacgac gaggctgcca tgggcattaa aagctgtgac cccaaaggcc
     1261 ctcttatgat gtatatttcc aaaatggtgc caacctccga caaaggtcgg ttctacgcct
     1321 ttggacgagt cttctcgggg ctggtctcca ctggcctgaa ggtcaggatc atggggccca
     1381 actatacccc tgggaagaag gaggacctct acctgaagcc aatccagaga acaatcttga
     1441 tgatgggccg ctacgtggag cccatcgagg atgtgccttg tgggaacatt gtgggcctcg
     1501 tgggcgtgga ccagttcctg gtgaagacgg gcaccatcac caccttcgag cacgcgcaca
     1561 acatgcgggt gatgaagttc agcgtcagcc ctgttgtcag agtggccgtg gaggccaaga
     1621 acccggctga cctgcccaag ctggtggagg ggctgaagcg gctggccaag tccgacccca
     1681 tggtgcagtg catcatcgag gagtcgggag agcatatcat cgcgggcgcc ggcgagctgc
     1741 acctggagat ctgcctgaag gacctggagg aggaccacgc ctgcatcccc atcaagaaat
     1801 ctgacccggt cgtctcgtac cgcgagacgg tcagtgaaga gtcgaacgtg ctctgcctct
     1861 ccaagtcccc caacaagcac aaccggctgt acatgaaggc gcggcccttc cccgacggcc
     1921 tggccgagga catcgataaa ggcgaggtgt ccgcccgtca ggagctcaag cagcgggcgc
     1981 gctacctggc cgagaagtac gagtgggacg tggctgaggc ccgcaagatc tggtgctttg
     2041 ggcccgacgg caccggcccc aacatcctca ccgacatcac caagggtgtg cagtacctca
     2101 acgagatcaa ggacagtgtg gtggccggct tccagtgggc caccaaggag ggcgcactgt
     2161 gtgaggagaa catgcggggt gtgcgcttcg acgtccacga cgtcaccctg cacgccgacg
     2221 ccatccaccg cggagggggc cagatcatcc ccacagcacg gcgctgcctc tatgccagtg
     2281 tgctgaccgc ccagccacgc ctcatggagc ccatctacct tgtggagatc cagtgtccag
     2341 agcaggtggt cggtggcatc tacggggttt tgaacaggaa gcggggccac gtgttcgagg
     2401 agtcccaggt ggccggcacc cccatgtttg tggtcaaggc ctatctgccc gtcaacgagt
     2461 cctttggctt caccgctgac ctgaggtcca acacgggcgg ccaggcgttc ccccagtgtg
     2521 tgtttgacca ctggcagatc ctgcccggag accccttcga caacagcagc cgccccagcc
     2581 aggtggtggc ggagacccgc aagcgcaagg gcctgaaaga aggcatccct gccctggaca
     2641 acttcctgga caaattgtag gcggcccttc ctgcagcgcc tgccgccccg gggactcgca
     2701 gcacccacag caccacgtcc tcgaattctc agacgacacc tggagactgt cccgacacag
     2761 cgacgctccc ctgagaggtt tctggggccc gctgcgtgcc atcactcaac cataacactt
     2821 gatgccgttt ctttcaatat ttatttccag agtccggagg cagcagacac gccctcttag
     2881 tagggactta atgggccggt cggggagggg gaggcgggat gggacaccca acactttttc
     2941 catttcttca gagggaaact cagatgtcca aactaatttt aacaaacgca ttaagaggtt
     3001 tatttgggta catggcccgc agtggctttt gccccagaaa ggggaaagga acacgcgggt
     3061 agatgatttc tagcaggcag gaagtcctgt gcggtgtcac catgagcacc tccagctgta
     3121 ctagtgccat tggaataata aatttgataa ggtggtga
//
