LOCUS       NM_004280               1047 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens eukaryotic translation elongation factor 1 epsilon 1
            (EEF1E1), transcript variant 1, mRNA.
ACCESSION   NM_004280
VERSION     NM_004280.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ding,L., Fang,Y., Li,Y., Hu,Q., Ai,M., Deng,K., Huang,X. and Xin,H.
  TITLE     AIMP3 inhibits cell growth and metastasis of lung adenocarcinoma
            through activating a miR-96-5p-AIMP3-p53 axis
  JOURNAL   J Cell Mol Med 25 (6), 3019-3030 (2021)
   PUBMED   33538115
  REMARK    GeneRIF: AIMP3 inhibits cell growth and metastasis of lung
            adenocarcinoma through activating a miR-96-5p-AIMP3-p53 axis.
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Kim,C., Park,J.M., Song,Y., Kim,S. and Moon,J.
  TITLE     HIF1alpha-mediated AIMP3 suppression delays stem cell aging via the
            induction of autophagy
  JOURNAL   Aging Cell 18 (2), e12909 (2019)
   PUBMED   30706629
  REMARK    GeneRIF: The AIMP3 enhanced mitochondrial respiration and
            suppressed autophagic activity, indicating that the
            AIMP3-associated modulation of metabolism and autophagy is a key
            mechanism in the senescence of stem cells and further suggesting a
            novel target for interventions against aging.
REFERENCE   3  (bases 1 to 1047)
  AUTHORS   Tao,Y., Fang,P., Kim,S., Guo,M., Young,N.L. and Marshall,A.G.
  TITLE     Mapping the contact surfaces in the Lamin A:AIMP3 complex by
            hydrogen/deuterium exchange FT-ICR mass spectrometry
  JOURNAL   PLoS One 12 (8), e0181869 (2017)
   PUBMED   28797100
  REMARK    GeneRIF: LmnA binds AIMP3 via its extreme C-terminus. Together
            these findings provide a structural insight for understanding the
            interaction between AIMP3 and LmnA in AIMP3 degradation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1047)
  AUTHORS   Cho,H.Y., Maeng,S.J., Cho,H.J., Choi,Y.S., Chung,J.M., Lee,S.,
            Kim,H.K., Kim,J.H., Eom,C.Y., Kim,Y.G., Guo,M., Jung,H.S.,
            Kang,B.S. and Kim,S.
  TITLE     Assembly of Multi-tRNA Synthetase Complex via Heterotetrameric
            Glutathione Transferase-homology Domains
  JOURNAL   J Biol Chem 290 (49), 29313-29328 (2015)
   PUBMED   26472928
  REMARK    GeneRIF: analysis of the heterotetrameric complex structure of the
            glutathione transferase (GST) domains shared among the four MSC
            components, methionyl-tRNA synthetase (MRS), glutaminyl-prolyl-tRNA
            synthetase (EPRS), AIMP2 and AIMP3
REFERENCE   5  (bases 1 to 1047)
  AUTHORS   Lee,S., Yu,K.R., Ryu,Y.S., Oh,Y.S., Hong,I.S., Kim,H.S., Lee,J.Y.,
            Kim,S., Seo,K.W. and Kang,K.S.
  TITLE     miR-543 and miR-590-3p regulate human mesenchymal stem cell aging
            via direct targeting of AIMP3/p18
  JOURNAL   Age (Dordr) 36 (6), 9724 (2014)
   PUBMED   25465621
  REMARK    GeneRIF: AIMP3 was shown to be involved in the cellular aging of
            human mesenchymal stem cells.
REFERENCE   6  (bases 1 to 1047)
  AUTHORS   Shiba,K., Stello,T., Motegi,H., Noda,T., Musier-Forsyth,K. and
            Schimmel,P.
  TITLE     Human lysyl-tRNA synthetase accepts nucleotide 73 variants and
            rescues Escherichia coli double-defective mutant
  JOURNAL   J Biol Chem 272 (36), 22809-22816 (1997)
   PUBMED   9278442
REFERENCE   7  (bases 1 to 1047)
  AUTHORS   Lamour,V., Quevillon,S., Diriong,S., N'Guyen,V.C., Lipinski,M. and
            Mirande,M.
  TITLE     Evolution of the Glx-tRNA synthetase family: the glutaminyl enzyme
            as a case of horizontal gene transfer
  JOURNAL   Proc Natl Acad Sci U S A 91 (18), 8670-8674 (1994)
   PUBMED   8078941
REFERENCE   8  (bases 1 to 1047)
  AUTHORS   Shiba,K., Suzuki,N., Shigesada,K., Namba,Y., Schimmel,P. and
            Noda,T.
  TITLE     Human cytoplasmic isoleucyl-tRNA synthetase: selective divergence
            of the anticodon-binding domain and acquisition of a new structural
            unit
  JOURNAL   Proc Natl Acad Sci U S A 91 (16), 7435-7439 (1994)
   PUBMED   8052601
REFERENCE   9  (bases 1 to 1047)
  AUTHORS   Kaiser,E., Hu,B., Becher,S., Eberhard,D., Schray,B., Baack,M.,
            Hameister,H. and Knippers,R.
  TITLE     The human EPRS locus (formerly the QARS locus): a gene encoding a
            class I and a class II aminoacyl-tRNA synthetase
  JOURNAL   Genomics 19 (2), 280-290 (1994)
   PUBMED   8188258
REFERENCE   10 (bases 1 to 1047)
  AUTHORS   Escalante,C. and Yang,D.C.
  TITLE     Expression of human aspartyl-tRNA synthetase in Escherichia coli.
            Functional analysis of the N-terminal putative amphiphilic helix
  JOURNAL   J Biol Chem 268 (8), 6014-6023 (1993)
   PUBMED   8449960
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC005291.1, AF054186.1,
            BG183264.1 and AI374688.1.
            
            On Nov 22, 2018 this sequence version replaced NM_004280.4.
            
            Summary: This gene encodes a multifunctional protein that localizes
            to both the cytoplasm and nucleus. In the cytoplasm, the encoded
            protein is an auxiliary component of the macromolecular
            aminoacyl-tRNA synthase complex. However, its mouse homolog has
            been shown to translocate to the nucleus in response to DNA damage,
            and it plays a positive role in ATM/ATR-mediated p53 activation.
            Alternative splicing results in multiple transcript variants.
            Read-through transcription also exists between this gene and the
            neighboring downstream MUTED (muted homolog) gene. An
            EEF1E1-related pseudogene has been identified on chromosome 2.
            [provided by RefSeq, Dec 2010].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189655.45773.1,
                                           SRR1660805.40425.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2144835, SAMN03267753
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000379715.10/ ENSP00000369038.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-586               BC005291.1         1-586
            587-837             AF054186.1         588-838
            838-1045            BG183264.1         11-218              c
            1046-1047           AI374688.1         10-11               c
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p24.3"
     gene            1..1047
                     /gene="EEF1E1"
                     /gene_synonym="AIMP3; P18"
                     /note="eukaryotic translation elongation factor 1 epsilon
                     1"
                     /db_xref="GeneID:9521"
                     /db_xref="HGNC:HGNC:3212"
                     /db_xref="MIM:609206"
     exon            1..114
                     /gene="EEF1E1"
                     /gene_synonym="AIMP3; P18"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..552
                     /gene="EEF1E1"
                     /gene_synonym="AIMP3; P18"
                     /note="isoform 1 is encoded by transcript variant 1;
                     aminoacyl tRNA synthetase complex-interacting
                     multifunctional protein 3; ARS-interacting multifunctional
                     protein 3; p18 component of aminoacyl-tRNA synthetase
                     complex; multisynthase complex auxiliary component p18;
                     multisynthetase complex auxiliary component p18"
                     /codon_start=1
                     /product="eukaryotic translation elongation factor 1
                     epsilon-1 isoform 1"
                     /protein_id="NP_004271.1"
                     /db_xref="CCDS:CCDS4507.1"
                     /db_xref="GeneID:9521"
                     /db_xref="HGNC:HGNC:3212"
                     /db_xref="MIM:609206"
                     /translation="MAAAAELSLLEKSLGLSKGNKYSAQGERQIPVLQTNNGPSLTGL
                     TTIAAHLVKQANKEYLLGSTAEEKAIVQQWLEYRVTQVDGHSSKNDIHTLLKDLNSYL
                     EDKVYLTGYNFTLADILLYYGLHRFIVDLTVQEKEKYLNVSRWFCHIQHYPGIRQHLS
                     SVVFIKNRLYTNSH"
     exon            115..315
                     /gene="EEF1E1"
                     /gene_synonym="AIMP3; P18"
                     /inference="alignment:Splign:2.1.0"
     exon            316..411
                     /gene="EEF1E1"
                     /gene_synonym="AIMP3; P18"
                     /inference="alignment:Splign:2.1.0"
     exon            412..1047
                     /gene="EEF1E1"
                     /gene_synonym="AIMP3; P18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      818..823
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EEF1E1"
                     /gene_synonym="AIMP3; P18"
                     /note="hexamer: AATAAA"
     polyA_site      850
                     /gene="EEF1E1"
                     /gene_synonym="AIMP3; P18"
                     /note="major polyA site"
     regulatory      1026..1031
                     /regulatory_class="polyA_signal_sequence"
                     /gene="EEF1E1"
                     /gene_synonym="AIMP3; P18"
                     /note="hexamer: AGTAAA"
     polyA_site      1047
                     /gene="EEF1E1"
                     /gene_synonym="AIMP3; P18"
ORIGIN      
        1 gcgtctgcca ggagctacgg ccggaagatg gcggcggccg cagagttgtc gctactggag
       61 aagtccctgg gactgagtaa ggggaataaa tacagtgctc agggcgagcg acagattcca
      121 gttcttcaga caaacaatgg tccaagtcta acaggattga ctactatagc agctcatcta
      181 gtcaagcaag ccaacaaaga atatttgctg gggagtactg cagaagaaaa agcaatcgtt
      241 cagcagtggt tagaatacag ggtcactcaa gtagatgggc actccagtaa aaatgacatc
      301 cacacactgt tgaaggatct taattcatat cttgaagata aagtctacct tacagggtat
      361 aactttacat tagcagatat actattgtac tatggacttc atcgctttat agttgacctg
      421 acagttcaag aaaaggagaa atatcttaat gtgtctcgct ggttttgtca cattcagcat
      481 tatccaggca tcaggcaaca tctgtctagt gttgtcttca tcaagaacag actatatact
      541 aattcccact agaagctgtc catgccatac agaagatcta ttaaaaatgt tttaaatgga
      601 aaatgtactc tagaccacag gactaatgta aattaatata cagtcattca ttatttgttg
      661 aagttgatag aatttttgaa gtgtaaactt gtgtctgaat gttttatttg ttctttagct
      721 gaagttttgc aatttttatg tcaaaattca attgctatta aacaagttga gatccagtta
      781 taaattaacc ttgtttttag tagatgacat ttatttcaat aaaagttgca aatcgggctt
      841 aatcttaaaa ttggtggtca tttcaatggt tgacatattt ggctatttat taacctctct
      901 ttcatattct aaaattcatt ttccccttat ggatatttat ggtagtttgt taagaactga
      961 taaattgtgc caaggaagcc aaaagggaag acagatggat ttgttttaaa atatttatgt
     1021 gagctagtaa atgtggttga aaaaata
//
